This document discusses various types of photon detectors, including vacuum phototubes, photomultiplier tubes, silicon photodiodes, photovoltaic cells, and multi-channel photon detectors. Photomultiplier tubes contain a cathode, anode, and dynodes that amplify the signal from incoming photons. Silicon photodiodes can operate in forward or reverse bias to detect photons. Photovoltaic cells use a semiconductor layer to generate a current from absorbed radiation. Multi-channel detectors like linear photodiode arrays allow simultaneous measurement of an entire light spectrum.
IR SPECTROSCOPY, INTRODUCTION, PRINCIPLE, THEORY, FATE OF ABSORBED RADIATION, FERMI RESONANCE, FINGERPRINT REGION, VIBRATIONS, FACTORS AFFECTING ABSORPTION OF IR RADIATION, SAMPLING TECHNIQUES, APPLICATIONS OF IR SPECTROSCOPY.
IR SPECTROSCOPY, INTRODUCTION, PRINCIPLE, THEORY, FATE OF ABSORBED RADIATION, FERMI RESONANCE, FINGERPRINT REGION, VIBRATIONS, FACTORS AFFECTING ABSORPTION OF IR RADIATION, SAMPLING TECHNIQUES, APPLICATIONS OF IR SPECTROSCOPY.
This presentation include the detailed explanation of various parts of a UV-Visible spectrophotometer and two types of UV-Visible spectrophotometers-Single beam and Doube beam. It also include the comparison between single beam and double beam spectrophotometers.
Detectors are the brain of any chromatograhic system. It help us to record the chromatogram based on certain characteristics of the analyte and help us in identifying that compound both qualitatively and quantitatively.
A method of obtaining an Infrared spectrum by measuring the interferogram of a sample using an interferometer, then performing a Fourier Transform upon the interferogram to obtain the spectrum.
In this slide contains Interference In Atomic Absorption Spectroscopy and applications.
Presented by: Shaik Gouse ul azam. ( department of pharmaceutical analysis.)
RIPER, anantpur.
describes the complete history, mechanisms, instrumentation(jablonski diagram), types, comparision and factors affecting, applications of fluorescence and phosphorescence and describes about quenching and stokes shift.
This presentation include the detailed explanation of various parts of a UV-Visible spectrophotometer and two types of UV-Visible spectrophotometers-Single beam and Doube beam. It also include the comparison between single beam and double beam spectrophotometers.
Detectors are the brain of any chromatograhic system. It help us to record the chromatogram based on certain characteristics of the analyte and help us in identifying that compound both qualitatively and quantitatively.
A method of obtaining an Infrared spectrum by measuring the interferogram of a sample using an interferometer, then performing a Fourier Transform upon the interferogram to obtain the spectrum.
In this slide contains Interference In Atomic Absorption Spectroscopy and applications.
Presented by: Shaik Gouse ul azam. ( department of pharmaceutical analysis.)
RIPER, anantpur.
describes the complete history, mechanisms, instrumentation(jablonski diagram), types, comparision and factors affecting, applications of fluorescence and phosphorescence and describes about quenching and stokes shift.
Photoelectric transducers and its classificationkaroline Enoch
The photoelectric transducer converts the light energy into electrical energy. It is made of semiconductor material. The photoelectric transducer uses a photosensitive element, which ejects the electrons when the beam of light absorbs through it.
spectrophotometer.pptx,DNA/RNA Quantification,clinical diagnosis, protein ana...Parthvi Soni
Spectrophotometry is a powerful analytical technique that plays a crucial role in scientific research and industrial applications. By measuring the absorbance of light by a sample, spectrophotometers provide valuable information about the concentration and characteristics of substances. Understanding the principles, components, and applications of spectrophotometry enables scientists and professionals to utilize this technique effectively for a wide range of analyses. As technology advances, spectrophotometry continues to evolve, offering greater precision, versatility, and efficiency in the pursuit of scientific knowledge and innovation.
High performance liquid chromatography (HPLC) head points:
HPLC Advantages Vs GC
Instrumentation
HPLC System
Separations
Mobile Phase Reservoirs
Degasser
Aim of Gradient system
High/Low pressure gradient system
HPLC Pump Criteria
HPLC Pumps: Types
Reciprocating Pumps
Sample introduction
Manual Injector
Auto Injector
HPLC Modes
The Mobile Phase
Hydrophobic interaction
Common reverse phase solvents
Detectors
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Gas chromatography head points:
Invention of Chromatography
original chromatography Experiment
Common types of chromatography
Paper and Thin layer chromatography
How does chromatography work?
Theoretical Plate
gas chromatography
schematic of GC
carrier gas-supply
Injection port
sample Injection system
split/spitless Injection
sample valves
GC columns
open tubular columns
Temperature Control
Solid Support Materials
Particle size of Supports
The stationary Phase
Detection systems
Characteristics of the Ideal Detector
Flame Ionization Detectors
Thermal Conductivity Detector
Electron-capture Detectors
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Theory and Principle of FTIR head points:
What is Infrared Region?
Infrared Spectroscopy
What is FTIR?
Superiority of FTIR
FTIR optical system diagram
sampling techniques
The sample analysis process
advantage of FTIR
References
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Scanning Probe microscopy (AFM and STM) head point
AFM: Configuration of AFM
Parts of AFM system and Principle of AFM
Three Modes of AFM
AFM Instrument
Advantage and disadvantage
STM
Schematic Diagram
AFM and STM
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X-Ray Diffraction head points:
Introduction
History
How Diffraction Works
Demonstration
Analyzing Diffraction Patterns
Solving DNA
Applications
Summary and Conclusions
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Photovoltaics: Fundamental Concepts and novel systems
Energy levels -bands
Doping of semiconductors
Energy band alignments between different phases
Space charge layers
p-n junctions, Schottky barriers
p-n cells, Si cells, thin film cells
Schottky cells (solid and liquid junction)
p-i-n cells
Fundamental limits of photovoltaic cells
How to overcome/ bypass these limits
New generation cells (brief survey)
PV stability, efficiencies and economics
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What is Automobile??
Brief History of Automobile
Changes over the years...
Indian Automobile Industry
Main parts of an Automobile
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Nanoimprint Lithography head points:
Approaches: thermal and UV NIL
Properties of NIL
Overview. of NIL
Thermal NIL resists.
Residual layer after NIL.
NIL for large features (more difficult than small one).
Room temperature NIL, reverse NIL, inking.
NIL of bulk resist (polymer sheet, pellets).
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Presenting a topic based on introduction to nanoscience and nanotechnology.
what is nano?
certain nomenclature like nanotechnology, nanoscience, nanomaterial, nanoscale, nanometer and so on.
surface to volume ratio and quantum effect related concepts.
future applications.
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Top down and bottom up method, processing, flow chart of top down and bottom up approach, Application.
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Different types of Nanolithography technique.
Types: Electron beam lithography, Photolithography, electron-beam writing, ion- lithography, X-ray lithography, and related images, concepts and graphical views.
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Infrared Spectroscopy and UV-Visible spectroscopyPreeti Choudhary
Instrumentation of Infrared Spectroscopy and UV-Vis spectroscopy
Discuss the fundamentals and concepts behind Infrared and UV-Vis spectroscopy.
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Presenting a presentation on the topic of Column chromatography with including basics of chromatography, principles, equations, graphs and data related to it.
Topics which covered in this ppt is
Principle of chromatography
classification of chromatography
partition coefficient
chromatogram
Resolution
plate theory
determination of N
band zone broadening
rate theory
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Column Chromatography: basics of chromatography and principle of chromatography, Classification, partition coefficient , chromatogram, retention time and volume, capacity and selectivity factors, plate theory, band broadening, rate theory, mass transfer, packed GLC column, open tubular column capillary columns, liquid chromatography column resolution.
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Operational amplifier: inverting and non-inverting amplifier, Power bandwidth, slew rate: slew rate distortion, noise gain, band width product. cascade amplifiers- bandwidth, CMRR, PSRR, Open loop op amp characteristics.
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Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
2. After the light has passed through the sample, we want to be able to
detect and measure the resulting light.
These types of detectors come in the form of transducers that are able to
take energy and convert it into an electrical signal that can be recorded,
and if necessary, amplified.
4. • This detector is a vacuum tube with a cesium-coated
photocathode- photoemissive in nature
•Photons of sufficiently high energy hitting the cathode
can dislodge electrons, which are collected at the wire
anode.
•Photon flux is measured by the current flow in the
system.
Phototube Detector
7. • Photomultiplier tube consists of a cathode, anode a few dynodes.
• Cathode supports a layer of photo emissive material such as an alkali metal
or metal oxide, that tends to emit electrons upon being irradiated.
• The emitted electrons are accelerated toward a dynode maintained at a
potential 90V more positive than the cathode.
• Upon striking the dynode surface, each accelerated photoelectron
produces several additional electron, all of which are then accelerated to
dynode 2, which is 90V more positive than dynode 1.
• Here again amplification occurs.
• By the time this process has been repeated at each of the remaining
dynodes, 10^6 to 10^7 electrons have been produced for each photon.
• This cascade is finally collected to the anode.
• The resulting current is then further amplified electronically and
measured.
8. Silicon Photodiodes
Forward biasing
• conduction takes place.
• small depletion region formed.
Reverse biasing
• non-conducting depletion layer is
formed.
• negligible flow of current.
10. • A reverse biased diode can serve as a radiation detector because UV
and visible photons are sufficiently energetic to create additional
electrons and holes when they strike the depletion layer of a pn junction.
•The resulting increase in conductivity is readily measured and is directly
proportional to radiant power.
• A silicon detector is more sensitive than a simple vacuum phototube but
less sensitive than a photomultiplier.
13. • It consists of a flat copper or iron electrode upon which is
deposited a layer of a semiconducting material (selenium oxide).
• The outer surface of the semiconductor is coated with a thin
transparent film of gold, silver, or lead, which serves as the collector
electrode.
• When radiation is absorbed on the surface of the semiconductor,
electron and holes are formed and migrate in opposite directions,
thus creating a current.
• If the two electrodes are connected with a low resistance circuit,
the current produced is directly proportional to the power of the
incident beam.
• The current can be measured through a simple microammeter.
15. MULTI CHANNEL PHOTON
DETECTORS
Consists of an array of tiny photosensitive detectors that are arranged in
such a pattern that all elements of a beam of radiation that has been
dispersed by a grating can be measured simultaneously
Three types of such devices-
1. Linear photodiode arrays
2. Vidicons
3. Charge transfer detectors
16. LINEAR PHOTODIODE ARRAYS
The flexibility, reliability, and cost-effectiveness of photodiode arrays have
taken these devices into an incredibly diverse range of applications, from
NASA satellites to clinical lab tests
OTHER APPLICATIONS:
X-ray baggage scanners
Position encoders
Particle measurement and counting systems
17.
18. Photodiode array
•PDA detector consists of a linear array of several hundred
photodiodes that have been formed along the length of silicon chip,
1-6cm in length.
•The individual widths of diodes are 15 to 50 µm
•The chip contains a capacitor and an electronic switch for each
diode
•Each capacitor is charged to -5V and the radiation impinging on
any diode surface causes partial discharge of its capacitor
•Resulting charging currents are proportional to the radiant power.
•Data for entire spectra are obtained in 1s or less
19. ADVANTAGES OF MULTI-
CHANNEL PHOTON DETECTORS
Simultaneous Multi-wavelength measurement
Fast scan speed
High signal to noise ratio
Wavelength precision
Minimal stray light effects
Ruggedness