The document summarizes cell theory and the key discoveries leading to its development. It describes the three main tenets of cell theory proposed by Schleiden, Schwann, and Virchow: 1) all organisms are composed of one or more cells, 2) the cell is the basic unit of life, and 3) all cells come from preexisting cells. It also discusses techniques like cell fractionation and chromatography used to isolate and study cellular components like organelles and proteins.
Centrifugation principle and types by Dr. Anurag YadavDr Anurag Yadav
concept of cnetrifugation,
basic Principle
centrifugal force
types of centrifugation based on use and rotor type
application of the each type of centrifuge
Ultracentrifuge in detail
application in general
Biological treatment is an important and integral part of any wastewater treatment plant that treats wastewater from either municipality or industry having soluble organic impurities or a mix of the two types of wastewater sources.
The four processes are: (1) Preliminary Treatment (2) Primary Treatment (3) Secondary or Biological Treatment and (4) Tertiary or Advanced Treatment
Centrifugation principle and types by Dr. Anurag YadavDr Anurag Yadav
concept of cnetrifugation,
basic Principle
centrifugal force
types of centrifugation based on use and rotor type
application of the each type of centrifuge
Ultracentrifuge in detail
application in general
Biological treatment is an important and integral part of any wastewater treatment plant that treats wastewater from either municipality or industry having soluble organic impurities or a mix of the two types of wastewater sources.
The four processes are: (1) Preliminary Treatment (2) Primary Treatment (3) Secondary or Biological Treatment and (4) Tertiary or Advanced Treatment
In his PPT you will come to know about the TREATMENT OF SOLID WASTE, ITS MANAGEMENT and MICROORGANISMS INVOLVED IN THE TREATMENT OF SOLID WASTE. do like, share and follow me to get more such PPT to be uploaded.
A fimbria (Latin for 'fringe', plural fimbriae), also referred to as an "attachment pilus" by some scientists, is an appendage that can be found on many Gram-negative and some Gram-positive bacteria, that is thinner and shorter than a flagellum. This appendage ranges from 3–10 nanometers in diameter and can be up to several micrometers long. Fimbriae are used by bacteria to adhere to one another and to adhere to animal cells and some inanimate objects. A bacterium can have as many as 1,000 fimbriae. Fimbriae are only visible with the use of an electron microscope. They may be straight or flexible.
A pilus (Latin for 'hair'; plural: pili) is a hair-like appendage found on the surface of many bacteria and archaea.[1] The terms pilus and fimbria (Latin for 'fringe'; plural: fimbriae) can be used interchangeably, although some researchers reserve the term pilus for the appendage required for bacterial conjugation. All pili in the latter sense are primarily composed of pilin proteins, which are oligomeric.
FOLLOW US ON YOUTUBE # BIOTECH SIMPLIFIED #
In his PPT you will come to know about the TREATMENT OF SOLID WASTE, ITS MANAGEMENT and MICROORGANISMS INVOLVED IN THE TREATMENT OF SOLID WASTE. do like, share and follow me to get more such PPT to be uploaded.
A fimbria (Latin for 'fringe', plural fimbriae), also referred to as an "attachment pilus" by some scientists, is an appendage that can be found on many Gram-negative and some Gram-positive bacteria, that is thinner and shorter than a flagellum. This appendage ranges from 3–10 nanometers in diameter and can be up to several micrometers long. Fimbriae are used by bacteria to adhere to one another and to adhere to animal cells and some inanimate objects. A bacterium can have as many as 1,000 fimbriae. Fimbriae are only visible with the use of an electron microscope. They may be straight or flexible.
A pilus (Latin for 'hair'; plural: pili) is a hair-like appendage found on the surface of many bacteria and archaea.[1] The terms pilus and fimbria (Latin for 'fringe'; plural: fimbriae) can be used interchangeably, although some researchers reserve the term pilus for the appendage required for bacterial conjugation. All pili in the latter sense are primarily composed of pilin proteins, which are oligomeric.
FOLLOW US ON YOUTUBE # BIOTECH SIMPLIFIED #
this presentation providing about the cell .Cell is the basic living, structural, and functional unit of the body.
Cells are grouped together to form tissues, each of which has a specialized function, e.g.- Bone and blood tissue.
Different tissues are grouped together to form a organs, e.g. liver, stomach, and kidney etc.
Organs are grouped together to form a system, each of which performs a particular function responsible for maintaining homeostasis .
e.g. Urinary system, Respiratory system etc.
Coronavirus disease 2019 (COVID-19). Complete information on coronavirus. Introduction, history, symptoms, covid19 structure, S protein of coronavirus, M proteins of coronavirus, spreading variations of coronavirus, vaccines, drugs to control coronavirus.
FOXP2 gene mutated in a speech and language disorder.
In humans, mutation of ‘FOXP2’ gene, results in a severe developmental disorder that significantly disrupts speech and language skills.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
Embracing GenAI - A Strategic ImperativePeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
Acetabularia Information For Class 9 .docxvaibhavrinwa19
Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
2. In 1838, Matthias Schleiden, a German botanist, concluded that,
plants were made of cells and that the plant embryo arose from a single
cell.
In 1839, Theodor Schwann, a German zoologist and colleague of
Schleiden’s, concluded that the cells of plants and animals are similar
structures.
Schleiden and Schwann proposed the cell theory:
The three tenets to the cell theory are as described below:
All organisms are composed of one or more cells.
The cell is the structural unit of life.
Cells arise from pre-existing cells.
3. Discovery of cells:
The cell was first discovered by Robert Hooke in 1665.
However, Hooke did not know their real structure or function.
During this time microscopes having a low magnification, Hooke
had observed the empty cell walls of plant tissue.
Hooke was unable to see that internal components to the cells.
4. Anton van Leeuwenhoek is another scientist who saw these cells
soon after Hooke.
He was made microscope containing improved lenses that could
magnify objects almost 300 (X) fold.
Under these microscopes, Leeuwenhoek found motile objects.
He states that motility is a quality of life therefore these were
living organisms.
5. Credit for developing cell theory is usually given to two scientists:
Matthias Jakob Schleiden AND Theodor Schwann.
1. All living organisms are composed of one or more cells
2. The cell is the most basic unit of life.
In 1855, Rudolf Virchow added the third tenet to cell theory.
3. All cells arise only from pre-existing cells
6. The modern version of the cell theory:
The modern version of the cell theory includes the ideas that:
1. Heredity information (DNA) is passed on from cell to cell.
1. Energy flow occurs within cells.
2. All cells have the same basic chemical composition.
8. There are two basic classes of cells prokaryotic and eukaryotic:
The eukaryotic cells almost certainly evolved from prokaryotic
ancestors.
Because of their common ancestry, both types of cells share an
identical genetic language.
a common set of metabolic pathways,
and many common structural features.
and both types of cells are bounded by plasma membranes.
9. Prokaryotic cell Eukaryotic cell
The size is 0.1- 5.0 µm The size is 5-100 µm
Cell wall, if present, contains
peptidoglycan
Cell wall, if present, contains
cellulose, pectin and chitin.
A typical nucleus is absent. A typical nucleus made of nuclear
envelope, chromatin, nucleoplasm,
nuclear matrix and nucleoli
DNA is generally circular. DNA is commonly linear
DNA is naked or without any
association with histone
proteins.
DNA is associated with histones.
10. Prokaryotic cell Eukaryotic cell
Introns are commonly absent in
DNA, RNA, therefore, does not
require splicing.
Introns are quite common. RNA,
therefore, requires spicing
process.
Cell membrane may have
infolding called Mesosomes.
Mesosomes absent
Mitochondria's are absent Mitochondria's are present
Ribosomes are 70 S Ribosomes are 80 S
Cell organelles absent Cell organelles present
11. Prokaryotic cell Eukaryotic cell
Sexual reproduction is
absent.
Sexual reproduction is present.
It may have pili and
fimbriae.
Pili and fimbriae are absent
Transcription occurs in the
cytoplasm
Transcription occurs inside the
nucleus.
Translation occurs in the
cytoplasm
Translation in the cytoplasm
13. Cell fractionation:
Cell fractionation is the process used to separate cellular
components.
Most cells contain a variety of different organelles, proteins and
enzymes.
To study a particular function of organelle or protein or enzyme,
first isolate the relevant organelle or protein or enzyme in a purified
state.
14. Isolation of a particular organelle in bulk quantity is generally
accomplished by the technique of differential centrifugation.
In centrifugation process particles of different size and shape
travel toward the bottom of a centrifuge tube at different rates
when placed in a centrifugal field.
15. To carry out this technique, cells are first broken by mechanical
disruption using a mechanical homogenizer.
The homogenate is then subjected to a series of sequential
centrifugations at increasing centrifugal forces.
Initially, the homogenate is subjected to low centrifugal forces for a
short period of time.
so that only the largest cellular organelles, such as nuclei, cell wall
debris (and any remaining whole cells), are sediment into a pellet.
17. At greater centrifugal forces, relatively large cytoplasmic
organelles (mitochondria, chloroplasts, lysosomes, and
peroxisomes) can be spun out of suspension.
In subsequent steps, microsomes and ribosomes are removed
from suspension.
This last step requires the ultracentrifuge, which can generate
speeds of 75,000 revolutions per minute, producing forces
equivalent to 500,000 times that of gravity.
18. Purification of a protein:
Chromatography techniques generally used for the separation of
proteins or enzymes from the homogenate.
Ion-exchange chromatography: Separate the proteins based on charge.
Gel Filtration Chromatography: Separate the proteins based on size.
19. Ion-exchange chromatography:
Ion-exchange chromatography depends on the ionic bonding of
proteins to an inert matrix material.
Two of the most commonly employed ion-exchange resins (inert
matrix material) are Diethylaminoethyl cellulose (DEAE) and
Carboxymethyl cellulose (CM).
DEAE-cellulose is positively charged and therefore binds
negatively charged molecules; it is an anion (-) exchanger.
CM-cellulose is negatively charged and acts as a cation (+)
exchanger.
21. The resin is packed into a column, and the protein solution is
allowed through the column in a buffer whose composition
promotes the binding of some or all of the proteins to the resin.
Proteins are bound to the resin reversibly and can be displaced
by increasing or changing the ionic strength (or pH) of the
buffer. (which adds small ions to compete with the charged
groups of the macromolecules for sites on the resin).
Proteins are eluted from the column in order from the least
strongly bound to the most strongly bound.
22. Gel Filtration Chromatography or size exclusion
chromatography :
Gel filtration separates proteins (or nucleic acids) primarily on the
basis of their effective size.
Like ion-exchange chromatography, the separation material consists
of gel beads that are packed into a column through which the
protein solution slowly passes.
The materials used in gel filtration are composed of cross-linked
polysaccharides (agarose or Sephadex G-150 beads) of different
porosity, which allow proteins to diffuse in and out of the beads.
23. For example if a solution consists of three different proteins such as 75 kDa and
25 kDa and 120 kDa.,
24. For example if a solution consists of three different proteins such as
120 kDa, 75 kDa and 25 kDa.
To purify 120 kDa protein form mixture, the sample pass through a
column of Sephadex G-150 beads.
When the protein mixture passes through the column bed, the 120
kDa protein is unable to enter the beads and remains dissolved in the
moving solvent phase.
The gel beads allows only the entry of proteins that are less than
about 100 kDa size.
25. As a result, the 120 kDa protein is eluted as soon as the preexisting
solvent in the column (the bed volume) has dripped out.
In contrast, the other two proteins can diffuse into the interstices
within the beads and are retarded in their passage through the
column.
As more and more solvent moves through the column, these proteins
move down its length and out the bottom, but they do so at different
rates.
Among those proteins that enter the beads, smaller species are
retarded to a greater extent than larger ones.
Consequently, the 120-kDa protein is eluted in a purified state, while
the 75-kDa and 25 kDa protein remains in the column.