Buffers for biochemical reactions
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This document discusses important considerations for choosing appropriate buffers for biochemical reactions. Effective buffers should not pass through biological membranes, minimally affect salt concentrations or be affected by changes in temperature or concentration. An optimal buffer will have a pKa between 6-8 to match most biochemical experiments, be water-soluble, and prepared at the correct temperature and concentration to avoid pH shifts. Choosing the right buffer is key to avoiding artifacts and ensuring experimental success.
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This document discusses how various factors affect the activity of enzymes. It examines how temperature, pH, and substrate concentration can impact the rate of enzymatic reactions. The text provides examples of how increasing temperature or adjusting pH can alter an enzyme's tertiary structure and influence its ability to catalyze reactions. It also explains that higher substrate concentrations generally correlate with increased reaction rates, as long as the enzyme amount remains constant. The document then describes an experiment that specifically tests the effects of temperature, pH, and substrate levels on the enzyme activity of peroxidase.
Bio bufferlab
Small changes in pH can distress organisms by altering chemical reaction rates in cells. The human blood pH range for survival is 7.0-7.8; outside this risks tiredness, trouble breathing, dizziness or disorientation. Buffers like carbonic acid in blood maintain pH homeostasis. Products like Alka-Seltzer use citric acid and sodium bicarbonate buffers to reduce stomach acidity. This lab investigates various materials' buffering capacities by measuring pH changes from acid and base additions.
pH, BUFFERS & ISOTONIC SOLUTIONS.pptx
This document discusses pH, buffers, and isotonic solutions. It begins by defining pH as a measure of hydrogen ion concentration in water using Sorensen's pH scale. It then describes two methods for determining pH: the calorimetric method which compares a solution's color to standard buffers and indicators, and the electrometric method which uses a pH meter. The document also discusses buffers and their importance in biological and pharmaceutical systems like blood, tears, and injections to maintain optimal pH levels. Factors that can influence a buffer's pH like temperature, dilution, and ionic strength are also covered.
Application of biopharmaceutics classification system in formulation developm...
Application of biopharmaceutics classification system in formulation developm...Surang Judistprasert
The document discusses the application of the Biopharmaceutics Classification System (BCS) in formulation development. It describes the four BCS classes based on a drug's solubility and permeability properties and how these properties impact bioequivalence studies and regulatory requirements. It also discusses factors that influence the predictability of in vivo performance based on the BCS class, such as first-pass metabolism and excipient effects. Recommendations are provided for designing successful bioequivalence studies using BCS principles.Unit First.pptx
This document discusses limitations of Lewis acid-base reactions, properties of buffer solutions, buffer capacity, the Henderson-Hasselbalch equation, and applications of buffers in pharmacy. It also covers general principles for adjusting solutions to isotonicity and its importance, types of impurities found in pharmaceutical substances, sources of impurities, effects of impurities, and limit tests for chloride, sulfate, iron, heavy metals, lead, and arsenic. Limit tests are used to identify and control small quantities of impurities that may be present in substances.
Measuring pKas, logP and Solubility by Automated titration
Presentation by Sirius Analytical covering measurement of pKa, LogP, LogD, Solubility, Supersaturation and precipitation kinetics.
For more details visit www.sirius-analytical.com
Problems with analysis of biological matrices
This document discusses factors affecting the stability of drugs and drug metabolites in biological matrices. It outlines various external factors like light, temperature, pH, oxidation, and enzymes that can impact stability. It also discusses structural factors such as ester pro-drugs and chiral interconversion. Specific examples are provided to illustrate how these different factors can influence stability and the importance of controlling for these factors during sample collection, storage, and processing. Approaches to improve stability are also described.
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Critical Success Factors for Optimization of Enzyme Linked Immunosorbent Assa...
Critical Success Factors for Optimization of Enzyme Linked Immunosorbent Assa...
Application of biopharmaceutics classification system in formulation developm...
Application of biopharmaceutics classification system in formulation developm...
Measuring pKas, logP and Solubility by Automated titration
Measuring pKas, logP and Solubility by Automated titration
More from Dr. sreeremya S
Offf shore wind park
Trends in the geometric size and rated power capacity of offshore wind turbines, the main components of an OWT system, encompassing a typical monopile foundation, the substructure, transition piece, the tower, rotor blades and nacelle (hub). Modern OWTs are installed with either pitch-regulated blades or variable rotational speed systems in order to allow optimisation of the power production over a wide range of prevailing wind speeds. The rotational speed of the main rotor shaft is typically between about 10 and 20 rp. Key electromechanical components of the wind turbine, including the gearbox and the generator (Roberts et al.,2007). The gearbox may cause efficiency losses for the wind turbine and is the particular source of the noise. Recent developments in the design of permanent magnet generators have made it possible to construct some types of wind turbines without the requirement for a gearbox. In this case, the rotor is mainly connected directly to a low-speed multi-pole generator that rotates at the same speed, termed the direct-drive unit. Removing the gearbox mainly removes one of the key components requiring more maintenance, and that is mainly prone to failure. This simplification of the mechanical part allows reductions in size and the mass of the nacelle (Gilling, 2009).
Plant based bifuel
The source of energy captured by plants is the sun, which will be the constant source of energy for the next few billion years. The carbon released from the burning of biofuels is continually cycled rather than being released from the ancient fixed carbon sources, as is the case for fossil petroleum and natural gas. The problem is that the cost of the production of fuels from lignocellulose and plant oils is high and this nascent industry cannot compete with the oil prices. Current progress: For the past two decades, ethanol has been synthesized primarily from cornstarch and cane sugar. Fourteen billion gallons of ethanol were synthesized in the USA from cornstarch in 2014. Approximately 40% of the current USA corn crop is availed to produce ethanol and is not likely to expand anymore, because the remainder of the crop is being availed for animal feed and human food. Ethanol is produced from cane sugar in Brazil at a level of 7.2 billion gallons in the year 2014. The renewable energy source is the major terrain to be considered (Sreeremya, 2019).
Agroforestry
Agroforestry has a high potential for simultaneously satisfying three important objectives viz., protecting and also stabilizing the ecosystems; producing a high level of output of economic goods; and improving the income and basic materials to the rural population. It has helped in the rehabilitation of the degraded lands on the one hand and has increased farm productivity on the other. At present, agroforestry meets almost half of the demand for fuelwood, 2/3 of the small timber, approx. 70-80 per cent wood for plywood, 60 per cent raw material for paper pulp and approx. 9-11 per cent of the green fodder requirement of livestock, besides meeting the subsistence needs of the households for food, fruit, fiber, medicine etc.
Phenylthiocarbamide (ptc)
Phenylthiocarbamide (PTC) is a bitter tasting chemical compound that some people can taste while others cannot. The ability to taste PTC is determined by a single gene and is inherited in a dominant fashion, with tasters having the genotype TT and non-tasters having tt. Studies have found differences in the frequency of the tasting allele between different human populations. Characterizing the exact PTC gene could help further examine its associations with food preferences and certain medical disorders.
Initial corrosion behavior of element copper in atmospheric environment
Copper and copper-based alloys encompassing brasses (Cu-Zn) and bronzes (Cu-Sn)are widely used in different industrial and the societal applications. They are common engineering materials in modern architecture and primarily availed for roofing and facade cladding due to their significance of visual appearance (important from an architectural perspective in terms of the design or during renovation of modern or ancient cultural building), ductility, malleability, the atmospheric corrosion resistance and long-term performance. When exposed to air, the copper forms a brownish-green or greenish blue corrosion layer, often denoted as the patina. Copper patina is commonly known as an aesthetically pleasing surface, and one reason for the extensive use of the copper metal and copper-based alloys in both the ancient and modern architecture. One of the most famous examples is the Statue of Liberty in the harbor of the New York, US.
Bronze alloys are the family of copper-based alloys traditionally alloyed with tin. Bronze alloys are of the exceptional historic interest and still finds wide applications.
Green chemistry
These approaches encompass new synthesis and processes as well as new tools for instructing aspiring chemists how to do the chemistry in a more environmentally benign manner. The pros to industry as well as the environment are all a part of the positive impact that Green Chemistry is having in the chemistry community and in the society in general. It is important that chemists develop novel Green Chemistry options even on an incremental basis. While all the elements of the lifecycle of a new chemical or process may not be environmentally benign, it is nonetheless pivotal to improve those stages where improvements can be made. The next phase of assessment can then focus on the elements of the lifecycle that are still in need of the improvement. Even though a new Green Chemistry methodology does not solve at once every problem allied with the lifecycle of a particular chemical or process, the advances that it does make are nonetheless very key. Green Chemistry that mainly possesses the spirit of sustainable development was booming in the 1990s
Thermoluminescence
Thermoluminescence is light emission from materials when they are heated after being exposed to radiation. This phenomenon was discovered in photosynthetic materials in the 1950s. The document discusses the use of thermoluminescence to study the effects of various chemicals on photosystem II activity in Chlorella cells. It finds good correlation between reduced photosystem II activity and algal growth inhibition for most chemicals. The document provides an overview of the history and development of using thermoluminescence to study photosynthesis, including identification of components involved in generating the glow curves observed. It describes how cooling and heating photosynthetic membranes in the dark produces light emission at distinct temperatures.
Corona virus current scenario
Corona virus was first identified as the cause of the common cold in 1960. In one study carried out in Canada in 2001, more than 510 patients presented with flu-like symptoms. Virological analyses showed that 3.7% of these cases were positive for the HCoV-NL63 strain by polymerase chain reaction (PCR). Until 2002, the corona virus was considered a relatively simple, nonfatal virus; however, an outbreak in 2002–2003 in the Guangdong province in China, which resulted in spread to many other countries, encompassing Thailand, Vietnam, Taiwan, Hong Kong Singapore, and the United States of America, causes severe acute respiratory syndrome (SARS) and high mortality rates in over 1000 -1100 patients.
Air purifying plants
In the modern times pollution has become the biggest menace for the survival of the biological species. There are different types of pollution e.g. air, water, soil, sound and mental pollution. Earth was the beautiful landscape but man has ruthlessly exploited for his greed specially, in the last century. With sporadic industrialization and random urbanization environmental pollution has become the serious problem. Over exploitation of open spaces, ever-increasing number of automobiles and the demographic pressure has further aggravated the problem. There are different ways and means to mitigate the urban environmental pollution. Plan-ting of trees and the shrubs for abatement of pollution and improvement of environment is a much effective way and well recognized throughout the world. Earlier, the purpose of planting trees in the urban areas was purely aesthetic (Sheldon et al., 1988a). The incessant increase of urban environmental pollution has necessitated to typically reconsider the whole approach of urban landscaping and its orientation in the order to achieve duel effect i.e. bio-aesthetics and mitigation of pollution.
Relation between haemorrhoids and constipation
Patients frequently complain of bleeding with or without the defecation, a swelling, mild discomfort or irritation. Other symptoms may include soilage or the mucous discharge, pruritis, difficulties with the hygiene, and a sense of incomplete evacuation. Internal hemorrhoids are otherwise painless unless they are mainly thrombosed, prolapsed with edema, or strangulated. External hemorrhoids result in pain when the thrombosis occurs and bleeding if ulceration occurs from pressure necrosis. Skin tags may form from the prior acutely edematous or the thrombosed external haemorrhoids (Johanson et al., 1990
Local food environment and obesity
Given the rapid aggrandize, it has been postulated the environment has a key role in the causation of obesity. Factors such as the neighborhood-scale features (referred to as the built environment) have been mainly identified as influencing diet and physical activity (2). Of particular the importance to this framework are features of the food environment (FE), thus constituting barriers and opportunities to food sources of the varying nutritional quality and energy density (3). The FE is delineated as the physical presence of food that can mainly influence a person’s diet (4). Contributing to the FE is the proximity to food store locations such as mainly the restaurants (fast-food, full service, and bars and pubs) and the retail outlets (food markets/grocery stores and liquor stores). Fast- food establishments generally have foods of lower nutritional quality and higher caloric density than the home-prepared foods (3). Early studies have reported fast-food restaurants to be more common in the neighborhoods with a higher prevalence of obesity. However, more recent studies have been melanged,
Adverse conditon provided for influenza virus (mutated
THIS MUTATED INFLUENZA VIRUS CAN BE TREATED IN PH1-2 . TO CHECK THE LYSIS OF THE VIRUS
SIMULATING THE INTERFERON ACTIVITY HAS TO BE DONE SIMULTANEOUSLY.
Cellulosic bmw
The document discusses cellulosic biomedical waste. It notes that while 76-90% of biomedical waste is non-hazardous, the remaining 10-26% is hazardous to humans, animals, and the environment if not handled properly. Major hospitals generate substantial amounts of biomedical waste, as do smaller facilities like nursing homes and clinics. Common disposal methods like incineration and landfilling are expensive and less environmentally friendly. The Government of India established rules in 1998 to regulate the handling and disposal of biomedical waste, including categories and proper procedures. Key steps for management include segregation by color, storage, transportation, and final treatment or disposal.
Corona virus current scenario (theoretical outlook)
This document discusses the coronavirus (COVID-19) pandemic. It provides background on coronaviruses, noting they were first identified in 1960 and can cause respiratory illnesses like SARS. The current coronavirus emerged in China in late 2019 and has since spread globally, killing hundreds daily. While studies on animals are limited, research aims to develop treatments like RNA silencing. Prevention efforts are led by CDC and WHO. Coronaviruses can spread through airborne droplets and contact with infected animals or humans. Further research is needed to fully understand transmission and develop effective treatments.
Kite turbine for wind energy
INTRODUCTION
As people have seen, the giant industrial wind farms, small household wind turbines, and also everything in between are erecting all over the places. In 2012, the electricity capacity from the wind power in the total electric capacity is about 7.2% in EU, 2.0% in China, and 3.6% in US. Nevertheless, most people agree that wind energy should claim much greater share of the future energy supply. For eg:-, the United States is working on an ambitious target of 21% total electricity capacity by 2030. Indeed, wind energy is one of the fastest growing energy sources today and also in the foreseeable future. However, findings a suitable site to build the wind farm or simply set up the wind turbine is not always easy. The wind must be strong and must be consistent; the tower cannot obstruct the view; and the noise cannot disturb the local residence. Due to the fast growth of the population, it is aggrandizingly difficult to find a suitable place that is proper for a wind turbine yet will not interfere with the people’s everyday life (Srensen et al., 2002).
Electro hybrid system
INTRODUCTION
A lot of basic concepts, technologies and pioneering solutions aiming on very high power densities were mainly developed within the last 20 years. Sometimes this is denoted as the mechatronic system integration or simply as mechatronics. Besides many of the technical aspects, the movings within the value chain represent the great challenge in this process. This is because traditionally defined between electronic device and the power module manufacturers, electronics assemblers, and the metalworking industry will blur or slowly disappear. However, the pressure to go this path increases. If one thinks, e.g., about the hybrid drive as an upgrade option for conventional passenger cars, one is directly faced with the lack of construction space as one of the main serious problems (Alkuhayli et al., 2012).
Excess maize in diet and pellagra
Pellagra may also occur as the result of a variety of (relatively rare) conditions affecting tryptophan metabolism and as a side-effect of the number of drugs that inhibit tryptophan metabolism. In alcoholics, it is unclear whether pellagra is the result of an impairment of tryptophan and the niacin metabolism directly attributable to alcohol, or whether it reflects general under nutrition among the people who obtain a considerable proportion on their energy needs from alcohol, and hence have the low intake of (nutrient rich) foods(Goldberger et al.,1922a).
SYMPTOMS
The major symptoms of pellagra are dermatitis, dementia, and diarrhea. This is because niacin deficiency is most noticeable in the body parts with high rates of cell turnover, such as the skin or gastrointestinal tract (Goldberger et al., 1923).
Combined effect of walking and forest
The growing interest in the environmental stress has been accompanied by a rapid accumulation of evidence indicating that the environment can elicit substantial stress in people living in urban environments. Furthermore, it is widely conceived that the natural environment can enhance human health. There have been several questionnaire studies and investigations on the psychological effects of forest environments. A previous study found an enhancement of the positive emotions among subjects who were shown pictures of natural environments. Moreover, other researches have also found that forest environments improve the psychological wellbeing of people
Liquid biopsies
As the techniques that have enabled us to analyses and assess a biopsy become ever more sophisticated, we have realised the limitations of looking at the single snap-shot of the tumour. This single-biopsy bias was highlighted in which it was mainly demonstrated that a portion taken from different parts of a primary tumour and its metastases showed and proved extensive intertumoural and intratumoural evolution. This tumoural heterogeneity mainly highlights the difficulty of dictating a therapeutic course of action based on the single biopsy, as it is likely to underestimate the ramification of the genomic landscape of the tumour (Chomczynski et al., 2016).Having established that there is mainly considerable tumour heterogeneity, taking multiple biopsies from the patients‘ primary tumour and the metastases would seem to be the most obvious next step. There are so much difficulties in obtaining the tissue biopsy— including the discomfort suffered by the patient, the inherent clinical risks to the patient
Rivivna humilis berry extract as fungal source
The introduction of the mankind had marked the continuous exploitation of the plants as organisms and their products for the pharmacological prospects and key features (Guha et al., 1999). In most of the agronomic domains around the globe, the conventional modes of the treatment are still in exercise and also in upcoming research (Srivastava et al., 2003).
These approaches most commonly exploit the various dynamics and much specific potential and applications of plant extracts (Bonde et al., 2007) . The distinctions of the first and foremost large-scale screening of the green plants evaluate and also assess the antimicrobial activity of 2,300 plants (Nautiyal et al., 1984).
Dyeing is an ancient and traditional art which predates written records. It was practised specifically during the Bronze Age in Europe (Sreeremya, 2016). Conventional dyeing techniques encompassed sticking plants to fabric or rubbing crushed pigments into cloth.
More from Dr. sreeremya S (20)
Initial corrosion behavior of element copper in atmospheric environment
Initial corrosion behavior of element copper in atmospheric environment
Adverse conditon provided for influenza virus (mutated
Adverse conditon provided for influenza virus (mutated
Corona virus current scenario (theoretical outlook)
Corona virus current scenario (theoretical outlook)
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Bob Reedy - Nitrate in Texas Groundwater.pdf
Presented at June 6-7 Texas Alliance of Groundwater Districts Business Meeting
Randomised Optimisation Algorithms in DAPHNE
Slides from talk:
Aleš Zamuda: Randomised Optimisation Algorithms in DAPHNE .
Austrian-Slovenian HPC Meeting 2024 – ASHPC24, Seeblickhotel Grundlsee in Austria, 10–13 June 2024
https://ashpc.eu/
Immersive Learning That Works: Research Grounding and Paths Forward
We will metaverse into the essence of immersive learning, into its three dimensions and conceptual models. This approach encompasses elements from teaching methodologies to social involvement, through organizational concerns and technologies. Challenging the perception of learning as knowledge transfer, we introduce a 'Uses, Practices & Strategies' model operationalized by the 'Immersive Learning Brain' and ‘Immersion Cube’ frameworks. This approach offers a comprehensive guide through the intricacies of immersive educational experiences and spotlighting research frontiers, along the immersion dimensions of system, narrative, and agency. Our discourse extends to stakeholders beyond the academic sphere, addressing the interests of technologists, instructional designers, and policymakers. We span various contexts, from formal education to organizational transformation to the new horizon of an AI-pervasive society. This keynote aims to unite the iLRN community in a collaborative journey towards a future where immersive learning research and practice coalesce, paving the way for innovative educational research and practice landscapes.
Equivariant neural networks and representation theory
Or: Beyond linear.
Abstract: Equivariant neural networks are neural networks that incorporate symmetries. The nonlinear activation functions in these networks result in interesting nonlinear equivariant maps between simple representations, and motivate the key player of this talk: piecewise linear representation theory.
Disclaimer: No one is perfect, so please mind that there might be mistakes and typos.
dtubbenhauer@gmail.com
Corrected slides: dtubbenhauer.com/talks.html
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
The technology uses reclaimed CO₂ as the dyeing medium in a closed loop process. When pressurized, CO₂ becomes supercritical (SC-CO₂). In this state CO₂ has a very high solvent power, allowing the dye to dissolve easily.
8.Isolation of pure cultures and preservation of cultures.pdf
Isolation of pure culture, its various method.
Applied Science: Thermodynamics, Laws & Methodology.pdf
When I was asked to give a companion lecture in support of ‘The Philosophy of Science’ (https://shorturl.at/4pUXz) I decided not to walk through the detail of the many methodologies in order of use. Instead, I chose to employ a long standing, and ongoing, scientific development as an exemplar. And so, I chose the ever evolving story of Thermodynamics as a scientific investigation at its best.
Conducted over a period of >200 years, Thermodynamics R&D, and application, benefitted from the highest levels of professionalism, collaboration, and technical thoroughness. New layers of application, methodology, and practice were made possible by the progressive advance of technology. In turn, this has seen measurement and modelling accuracy continually improved at a micro and macro level.
Perhaps most importantly, Thermodynamics rapidly became a primary tool in the advance of applied science/engineering/technology, spanning micro-tech, to aerospace and cosmology. I can think of no better a story to illustrate the breadth of scientific methodologies and applications at their best.
ESR spectroscopy in liquid food and beverages.pptx
With increasing population, people need to rely on packaged food stuffs. Packaging of food materials requires the preservation of food. There are various methods for the treatment of food to preserve them and irradiation treatment of food is one of them. It is the most common and the most harmless method for the food preservation as it does not alter the necessary micronutrients of food materials. Although irradiated food doesn’t cause any harm to the human health but still the quality assessment of food is required to provide consumers with necessary information about the food. ESR spectroscopy is the most sophisticated way to investigate the quality of the food and the free radicals induced during the processing of the food. ESR spin trapping technique is useful for the detection of highly unstable radicals in the food. The antioxidant capability of liquid food and beverages in mainly performed by spin trapping technique.
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Thornton ESPP slides UK WW Network 4_6_24.pdf
ESPP presentation to EU Waste Water Network, 4th June 2024 “EU policies driving nutrient removal and recycling
and the revised UWWTD (Urban Waste Water Treatment Directive)”
Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...
The pygmy halfbeak Dermogenys colletei, is known for its viviparous nature, this presents an intriguing case of relatively low fecundity, raising questions about potential compensatory reproductive strategies employed by this species. Our study delves into the examination of fecundity and the Gonadosomatic Index (GSI) in the Pygmy Halfbeak, D. colletei (Meisner, 2001), an intriguing viviparous fish indigenous to Sarawak, Borneo. We hypothesize that the Pygmy halfbeak, D. colletei, may exhibit unique reproductive adaptations to offset its low fecundity, thus enhancing its survival and fitness. To address this, we conducted a comprehensive study utilizing 28 mature female specimens of D. colletei, carefully measuring fecundity and GSI to shed light on the reproductive adaptations of this species. Our findings reveal that D. colletei indeed exhibits low fecundity, with a mean of 16.76 ± 2.01, and a mean GSI of 12.83 ± 1.27, providing crucial insights into the reproductive mechanisms at play in this species. These results underscore the existence of unique reproductive strategies in D. colletei, enabling its adaptation and persistence in Borneo's diverse aquatic ecosystems, and call for further ecological research to elucidate these mechanisms. This study lends to a better understanding of viviparous fish in Borneo and contributes to the broader field of aquatic ecology, enhancing our knowledge of species adaptations to unique ecological challenges.
Sharlene Leurig - Enabling Onsite Water Use with Net Zero Water
Sharlene Leurig - Enabling Onsite Water Use with Net Zero WaterTexas Alliance of Groundwater Districts
Presented at June 6-7 Texas Alliance of Groundwater Districts Business MeetingThe debris of the ‘last major merger’ is dynamically young
The Milky Way’s (MW) inner stellar halo contains an [Fe/H]-rich component with highly eccentric orbits, often referred to as the
‘last major merger.’ Hypotheses for the origin of this component include Gaia-Sausage/Enceladus (GSE), where the progenitor
collided with the MW proto-disc 8–11 Gyr ago, and the Virgo Radial Merger (VRM), where the progenitor collided with the
MW disc within the last 3 Gyr. These two scenarios make different predictions about observable structure in local phase space,
because the morphology of debris depends on how long it has had to phase mix. The recently identified phase-space folds in Gaia
DR3 have positive caustic velocities, making them fundamentally different than the phase-mixed chevrons found in simulations
at late times. Roughly 20 per cent of the stars in the prograde local stellar halo are associated with the observed caustics. Based
on a simple phase-mixing model, the observed number of caustics are consistent with a merger that occurred 1–2 Gyr ago.
We also compare the observed phase-space distribution to FIRE-2 Latte simulations of GSE-like mergers, using a quantitative
measurement of phase mixing (2D causticality). The observed local phase-space distribution best matches the simulated data
1–2 Gyr after collision, and certainly not later than 3 Gyr. This is further evidence that the progenitor of the ‘last major merger’
did not collide with the MW proto-disc at early times, as is thought for the GSE, but instead collided with the MW disc within
the last few Gyr, consistent with the body of work surrounding the VRM.
THEMATIC APPERCEPTION TEST(TAT) cognitive abilities, creativity, and critic...
THEMATIC APPERCEPTION TEST(TAT) cognitive abilities, creativity, and critic...Abdul Wali Khan University Mardan,kP,Pakistan
hematic appreciation test is a psychological assessment tool used to measure an individual's appreciation and understanding of specific themes or topics. This test helps to evaluate an individual's ability to connect different ideas and concepts within a given theme, as well as their overall comprehension and interpretation skills. The results of the test can provide valuable insights into an individual's cognitive abilities, creativity, and critical thinking skillsMicronuclei test.M.sc.zoology.fisheries.
Current Ms word generated power point presentation covers major details about the micronuclei test. It's significance and assays to conduct it. It is used to detect the micronuclei formation inside the cells of nearly every multicellular organism. It's formation takes place during chromosomal sepration at metaphase.
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mô tả các thí nghiệm về đánh giá tác động dòng khí hóa sau đốt
mô tả các thí nghiệm về đánh giá tác động dòng khí hóa sau đốt
GBSN - Biochemistry (Unit 6) Chemistry of Proteins
GBSN - Biochemistry (Unit 6) Chemistry of Proteins
Immersive Learning That Works: Research Grounding and Paths Forward
Immersive Learning That Works: Research Grounding and Paths Forward
Equivariant neural networks and representation theory
Equivariant neural networks and representation theory
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
8.Isolation of pure cultures and preservation of cultures.pdf
8.Isolation of pure cultures and preservation of cultures.pdf
Applied Science: Thermodynamics, Laws & Methodology.pdf
Applied Science: Thermodynamics, Laws & Methodology.pdf
ESR spectroscopy in liquid food and beverages.pptx
ESR spectroscopy in liquid food and beverages.pptx
Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...
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Buffers for biochemical reactions
- 1. Buffers for Biochemical Reactions BY SREEREMYA.S
- 2. Buffers often are overlooked and taken for granted by laboratory scientists until the day comes when a bizarre artifact is observed and its origin is traced to a bad buffer. Although mistakes in the composition of buffers have led occasionally discoveries such as the correct number of human chromosomes (Arduengo, 2010), using the proper buffer, correctly prepared, can be key to success in the laboratory
- 3. Exclusion by biological membranes. This is not important for all biochemical reactions. However, if this is an important criterion for your particular experiment, it is helpful to remember that zwitterionic buffers (positive and negative charges on different atoms within the molecule) do not pass through biological membranes. Examples of zwitterionic buffers include MOPS and HEPES; Tris and phosphate buffers do not isomerize into zwitterions.
- 4. Minimal salt effects. In other words, the buffer components should not interact or affect ions involved in the biochemical reactions being explored.
- 5. Minimal effects on the dissociation from changes in temperature and concentration. Usually there is some change in the dissociation with a change in concentration. If this change is small, stock solutions usually can be diluted without changing the pH. However, with some buffers, changes in concentration have more effect on dissociation, and stock solutions cannot be diluted without significantly affecting pH. For instance, the pH of Tris decreases approximately 0.1 pH unit per tenfold dilution, and the pH could change dramatically if you dilute a working solution and are at the limits of the optimal buffering range of the Tris (optimal buffering range pH 7.3–9.3 at 20°C). Note that Tris is not one of Good’s buffers.
- 6. A pKa between 6 and 8. Most biochemical experiments have an optimal pH in the range of 6–8. The optimal buffering range for a buffer is the dissociation constant of the weak acid component of the buffer (pKa) plus or minus pH unit. • Solubility in water. Biological reactions, for the most part, occur in aqueous environments, and the buffer should be water-soluble for this reason.
- 7. Prepare Buffers at the Appropriate Temperature and Concentration Because changes in temperature can be associated with a shift in dissociation, prepare your buffers at the temperature at which you will be performing your experiments. If your experiment involves a change in temperature, choose a buffer with a pKa that accommodates it. Changes in concentration also can be associated with a shift in dissociation, so if you plan to maintain buffer stock solutions, make sure that the pH adjustment is made after you have diluted the stock to the desired concentration and equilibrated it at the appropriate temperature. Or, at the very least, check the pH after dilution.