This document summarizes research on breeding for disease resistance in maize. It discusses the major maize diseases that affect production in different agro-ecological zones in sub-Saharan Africa. The need to manage diseases to prevent economic losses is explained. Constraints and focus diseases for the lowlands and mid-high altitudes are outlined. The objective of the research is to identify superior disease resistant germplasm for breeding programs using multi-location phenotyping and association mapping approaches. Methods for screening germplasm under natural and artificial epidemics are described. Promising sources of resistance identified for gray leaf spot, maize streak virus, northern corn leaf blight, and rust are presented.
The most troublesome pests of paddy along with their control measures
For more information :
visit the link below:
http://infentfun.blogspot.in/p/blog-page_17.html
The most troublesome pests of paddy along with their control measures
For more information :
visit the link below:
http://infentfun.blogspot.in/p/blog-page_17.html
Seed borne diseases are caused by micro-organisms infecting seeds. Seeds are attacked by various fungi, bacteria and viruses at various stages viz., in the field ,during processing, at the time of transportation, and during storage.
INTRODUCTION
OCCURENCE AND IMPORTANCE
DIFFERENT TYPES OF WHEAT RUST
BLACK RUST
BROWN RUST
YELLOW RUST
COMPARISION OF ALL THREE RUST
SYMPTOMS
SIGNIFICANCE
HISTORY
RUST CYCLE
STAGES OF PATHOGEN
EPIDEMIOLOGY
RUST CYCLE IN INDIA
UG99
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The Integrated Disease Management (IDM) involves the need based use of pesticide only when the disease incidence reach economic threshold levels and this will promote the build up of many bio-control agent in the crop ecosystems.
Thus IDM, a greener alternative to the conventional use of chemicals, is an attempt to promote natural, economic and sociological farming methods through the most effective combination of farming techniques and judicious and limited use of fungicide.
In other words, IDM programme implies all the available disease management approaches including cultural, biological and chemical control with the main objective to keep the disease incidence below economic threshold level.
Seed borne diseases are caused by micro-organisms infecting seeds. Seeds are attacked by various fungi, bacteria and viruses at various stages viz., in the field ,during processing, at the time of transportation, and during storage.
INTRODUCTION
OCCURENCE AND IMPORTANCE
DIFFERENT TYPES OF WHEAT RUST
BLACK RUST
BROWN RUST
YELLOW RUST
COMPARISION OF ALL THREE RUST
SYMPTOMS
SIGNIFICANCE
HISTORY
RUST CYCLE
STAGES OF PATHOGEN
EPIDEMIOLOGY
RUST CYCLE IN INDIA
UG99
Here is the brief description of guava wilt and cotton wilt. This presentatation was presented by Bipin karki,BIjendra pradhan, Bivek Gyawali and Dorna Giri at IAAS Paklihawa.
The Integrated Disease Management (IDM) involves the need based use of pesticide only when the disease incidence reach economic threshold levels and this will promote the build up of many bio-control agent in the crop ecosystems.
Thus IDM, a greener alternative to the conventional use of chemicals, is an attempt to promote natural, economic and sociological farming methods through the most effective combination of farming techniques and judicious and limited use of fungicide.
In other words, IDM programme implies all the available disease management approaches including cultural, biological and chemical control with the main objective to keep the disease incidence below economic threshold level.
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MLN status, Disease diagnosis and Management Kitale, Kenya 23rd June 2022.pptxSuresh, L.M
MLN diagnosis and disease management training is given to NPPO's across in ESA to help them for understanding MLN disease. This also helps them for disease diagnosis and produce disease free seeds and manage the disease. Thus it helps for good food security. Recently the training wasprovided. This shall help a large audians
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Breeding for disease resistance in maize new breeders course - lusaka zambia 25 aug 2015 final
1. Breeding for disease resistance in Maize
Stephen Mugo, George Mahuku, Dan Makumbi and C. Magorokosho, Suresh L.M.
Presentation made to the New Maize Breeder’s Training Course,
Lusaka, Zambia, 17 August – 4 Sept, 2015
2. Maize Diseases
Maize production in sub-Saharan Africa is affected
by a wide array of diseases
Environmental conditions prevalent in the different
agro-ecological zones are conducive to the growth
and spread of pathogens
Different disease complexes affect maize production
in the lowlands and mid-high altitudes
Diseases often reduce production and cause up to
100% yield loss under severe epidemics depending
on environmental conditions
3. Need to manage disease in maize
• Prevent economic losses
– Reduced yields
– Increased production
costs
– Poor quality grain
• Reduce or eliminate the
risks associated with
presence of a disease
• Guarantee food security
4. Constraints Lowlands Mid-altitude-highlands
Foliar diseases (reduce photosynthetic area)
Gray leaf spot (Cercospora zeae-maydis) x
Northern corn leaf blight (Exserohilum turcicum) x
Southern corn leaf blight (Bipolaris maydis) x
Common rust (Puccinia sorghi) x
Southern rust (Puccinia polysora) x
Downy mildew (Peronosclerospora sorghi) x
Maize streak virus disease x x
Ear rots (reduce quality of maize grain)
Diplodia ear rot (Stenocarpella maydis) x
Aspergillus ear rot (Aspergillus flavus) x x
Fusarium ear rots (Fusarium moniliforme) x x
Stalk rots (cause premature death of plants)
Diplodia stalk rot (Diplodis maydis) x
Fusarium ear rots (Fusarium moniliforme) x x
Charcoal rot (Macrophomina phaseoli) x
x =prevalent in the zone
Major maize diseases that significantly reduce maize
production in different ecological zones in SSA
8. Objective
• Identify superior disease resistant germplasm for
incorporation into breeding programs
• Collect good disease phenotypic data
• Use association mapping approaches to
understand the organization of disease resistance
genes in the maize genome
• Develop markers for marker assisted selection
breeding
9. Research Strategy
• High precision multi-location phenotyping:
– identify good sources of resistance for use as donors
– Validate stability of resistance genes
• Association mapping studies
– Leverage the DTMA AM set to identify chromosomal
regions involved in disease resistance
– Organization of disease resistance genes on maize
genome
• Fine mapping pipeline to develop functional
markers
– DH lines
– F2.3, BC1, BC2, BC3 populations
10. Breeding for resistance to diseases
Use of disease resistant cultivars is the most valuable and
practical means to control diseases
It is also inexpensive, effective, and simple to apply over a
wide area in a target production zone
Requirements for development of disease resistant maize
cultivars
1. Diverse germplasm
2. Screening tools
3. Test locations with consistently high disease pressure
11. Resistance is available for most of the economically important
diseases in maize
Resistance is controlled mainly by
One or a few genes (monogenic or oligogenic)
Many genes (polygenic)
With additive and dominance effects
1.Vertical resistance
Complete resistance of a host to a specific race of a pathogen
The host plant exhibit hypersensitive reaction that prevents
the establishment and multiplication of the pathogen
Controlled by one (monogenic) or a few (oligoginic) genes
Plants show distinct resistant and susceptible categories
Selection is thus easy in segregation populations
Transfer from source to other germplasm is also easy
It is less durable
Has been used to control very few disease in maize
Types of resistance
12. 2. Horizontal resistance
Effect of resistance on the survival and reproduction of he
pathogen is less complete
Provides less selection pressure on the pathogen
It retards the infection process and slows down the spread of
the disease
Controlled by many genes (polygenic) each with small effect
Resistance shows continuous variation
It is more durable and stable due to the buffering effect of
polygenes
Has been used for controlling most diseases in maize
The two types of resistance can co-exist
Types of resistance
13. Factors affecting disease occurrence
* Climate change impacts the host, agent (pathogen) and environment
Environment
(favorable)
Pathogen
(virulent)
Host (Susceptible)
Man
•Temperature
•Relative humidity
•Rainfall
•Dew
•Solar radiation
14. Mechanisms for disease resistance
1. Resistance to pathogen establishment
•Immunity
– Prevent pathogen from establishing itself due to innate
structural or functional properties of the host
•Hypersensitivity
– Prevents pathogen survival and reproduction due to rapid
death of the host plant cells
2. Resistance to an established pathogen
– Restricts the ability of the pathogen to spread and
reproduce after becoming established in a host
3. Tolerance
– The plant exhibits severe disease symptoms without a
serious loss in yield
15. Availability of diverse germplasm for screening as
sources of resistance
• Sufficient genetic variation exists for most of the
diseases in maize
• Locally adapted or introduced maize germplasm
• Old varieties and breeding stocks
• Landrace collections
• Resistance alleles in these genetic resources can occur
at low or high frequencies
• Resistance genes occurring at low frequencies can be
gradually increased
• Genes at high frequency are easy to transfer
16. Based on reliable identification of good sources of
resistance
This can be done through:
Development of standardized, highly efficient inoculation techniques and
disease rating systems for major maize diseases that include:
Fusarium ear rot (FSR); Fusarium stalk rot (FSR); Gray
leaf spot (GLS); Tar spot complex (TSC); and Turcicum
leaf blight (TLB)
Establishment of misting systems at key and crucial sites to create
microclimatic conditions suitable for disease development.
Identification of disease hot spot sites, known for consistent, uniform and
reliable disease incidence and pressure.
Approach to minimizing production losses from
diseases
17. Resistance screening methods
• Field, greenhouse (screenhouse) and laboratory-based
screening techniques are available for the major
diseases of maize
• Use established screening techniques
Effective
Cheap
Easy to handle depending on available facilities and
personnel
High throughput for screening a large number of
breeding materials
• Field screening of breeding nurseries at hot-spot
locations with consistently high disease pressure is
also effective
• Evaluate selected resistant genetic materials in one
18. Test entries are exposed to adequate and uniform
disease pressure.
Guarantee greatest differentiation of genotypes.
Objective of disease evaluations
19. Rate of progress to develop stable and durable
disease resistance or marker development depends
on:
the use of reliable screening techniques
use of as wide a spectrum of the pathogen as possible and at an
appropriate disease pressure
Take note that:
Low disease pressure
Unreliable results that slow down rate of genetic
gain
High or severe disease pressure
Eliminate low level resistance inherent in adapted
germplasm and may drastically narrow the
germplasm base
Disease screening methods
20. Two major groups:
1. Naturally occurring epidemics
2. Artificially created epidemics
Disease screening techniques
21. Naturally occurring epidemics
Hot spots
Use of a location known for its high level of infection for a
particular disease
Used for a pathogen with a local concentration of alternate
hosts
Advantages
Cheap and easy to manage
Test materials are exposed to all pathogen races
Disadvantages
Success depends on year– to– year consistent expression of
epiphytotics
adequate and uniform natural infections can rarely be
achieved in most locations
Disease might not be evenly distributed within the field
22. Naturally occurring epidemics
Enhanced natural infections to ensure adequate
disease levels
Manipulation of planting dates
Create favorable environmental conditions (e.g.
irrigation, enhanced drought etc)
Use of spreader rows & use susceptible checks every
few rows (e.g. every tenth row)
Sufficient replications (minimum of three)
Multiple locations
24. Parameter Batan
(2008)
Batan
(2009)
Batan
(2010)
Combined
location
Entry Variance 0.92 0.69 1.28 0.45
Residual variance 0.39 0.31 0.17 0.28
Heritability 0.82 0.87 0.96 0.85
LSD -05 1.24 0.92 0.66 0.91
CV 22.53 25.50 12.12 20.40
Resistant Susceptible
Replications =3
Number of entries = 300 genotypes
Good phenotyping data across locations –
Common Rust (Puccinia sorghi)
Oxalis sp. – an alternative host for Puccinia sorghi
25. Artificially created epidemics
Environmental conditions favorable for optimal
disease development rarely occur every year
Great variation in the severity of the disease within a
location in a year
Ensure adequate epidemic development
Versatile - Can be done in laboratory, greenhouse
and field
26. Inoculum production in
the maize pathology
laboratory in CIMMYT.
The inoculum is
produced on colonized
sorghum grain and
used for artificial
inoculations of leaf
diseases of maize (TLB,
MLB).
Inoculations done at
the 6-8 leaf stage.
Colonized sorghum
seed serves as sources
of inoculum for 7
weeks under field.
27. In the case of Fusarium ear rot -
Steps for preparing inoculum
29. Disease phenotyping hubs
Harare
Kakamega / Embu
Misting system
Environment conditions that
inherently favors expression of
plant diseases
Protocols
Well developed and available
for many disease
Issues
Lack of standardization
Common checks
Limits harmonization of data
from different organizations
Protocols for reliable disease phenotyping
30. Evaluation – Resistance vs. susceptible
Data loggers Inoculation technique
Fieldbook and fieldlog
Parameters for reliable disease phenotyping
31. Various degree of TLB infection on
different genotypes of maize despite of
using same inoculum, same inoculation
and observation time. Therefore, it
requires standardized disease rating
scale.
Disease evaluation
36. Multi-location Disease Phenotyping
Phenotyping Site MSV GLS Et Ear rots Ps PP BM
Harare, Zimbabwe X X X
Mpongwe, Zambia X
Kakamega, Kenya X X X
Embu, Kenya X X X
Kibos, Kenya X X
Catalina, Colombia X X
El Batan, Mexico X X X
Agua Fria, Mexico X X X X
San Pedro , Mexico X
Acatic, Mexico X
= Natural condition; = Artificial condition
37. P = 0.0001
EM-KN BA-Mex1 BA-Mex2 SL-Mex
EM-KN 1
BA-Mex1 0.86 1
BA-Mex2 0.87 0.96 1
SL-Mex 0.99 0.99 0.98 1
Phenotypic correlations between sites for
common rust
42. Conclusions
• A large number of inbred lines, open-pollinated
varieties, hybrids, and source population with
resistance to the major diseases are available for
use
– As sources of alleles to breed maize for resistance to the
major diseases
• Other disease for which artificial inoculations are
conducted / protocols available
– Ear rots (Fusarium and Aspergillus), Stalk Rots, turcicum
blight, southern corn leaf blight, common rust.
43. Conclusions
Information on sources of disease resistance on
CIMMYT website & available to collaborators
Establishing diseases phenotyping network comprised
of different institutions and seed companies etc.
Build capacity of collaborators on use of harmonized
disease evaluation protocols
Poor grain quality can be from direct damage or production of mycotoxins
Eliminate or reduce the risk that is associated with the occurrence of diseases. This will prevent you from proper planning, as production would flatuate from year to year, and prevent farmers from proper planning. For example, when a cultivar yields 9
Clearly distinguish resistant from susceptible. Failure to do this, reduces the rate of genetic gain to development of stable hybrids or cultivars.
Oxalis – alternate host for Puccinia sorghi
High genetic variability of the pathogen, so materials are exposed to the full spectrum of pathogen variability. Therefore, resistant germplasm holds across environments, as they will have been exposed to the greatest genetic variability available.
There are several good hot spots for diseases i.e. areas where the disease pressure and conducive environments for disease development are available year after year. For example, El Batan in Mexico is a good hot spot for common rust, as the alternate host is available so genetic variability of the pathogen is maintained.
Materials that are resistant under these conditions are usually resistant in all other locations. Use of infected crop residue as source of inoculum
Artificial inoculations requires the isolation and characterization of the pathogen, production of inoculum and inoculations in the field. Although very useful, it is a cumbersome technique requiring expertise in pathology.
Artificial inoculations depends on the pathogen to be inoculated, the stage of plant growth and availability of conducive environmental conditions.
This requires knowledge of both the host, pathogen and prevailing conditions for success.
Inoculations at the right time is key to success.
Degree of mode of infection is completely depend on host genotypes whether compatible or incompatible to the pathogen
Proper rating is important to correctly identify or distinguish resistant from susceptible germplasm.
Use of common scales is important for harmonizing data coming from different scientists. This will increase the rate of genetic gains in developing resistant varieties. When different scales are used, the data can not be combined into a single dataset from multiple environments.
Most researchers use different scales which means different things, so it is not possible to combine the data. In CIMMYT, we are developing common protocols to share with our partners so that when we receive a data set with a certain rating, we know exactly what this means.
Example of a rating protocol that we are developing for common rust, to be used as a reference guide and reduce bias.
The most abused system
Almost every scientist uses their own scales, at times not even harmonize within institutions (unlike major companies eg Pioneer)
How then do we harmonize our data and make it more powerful if we use different scales?
An example of the rating scale showing the different scales and what they mean, from 1 = no disease to 5 the plant is >80% diseased and is completely blighted.
As mentioned before, proper disease evaluations is key. If you get poor data, your conclusions will be compromised and this will reduce the rate of genetic gains to develop resistant maize varieties and hybrids.
High genetic correlations for phenotypic data across locations revealing that germplasm resistant in one location is also resistant in other locations.
Therefore, very little genetic variability in common rust pathogen or the variability is sufficiently sampled.
Selection and characterization of disease hot spots is crucial. If a good site is identified, the disease data is highly repeatable, and increases confidence in selection of resistant germplasm.
Example of GLS hot spots in Nayarit and Jalisco and Colombia. The repeatability of the trial with 3 replication is >80% showing that the disease is uniformly distributed, and germplasm performed consistently across replications.