The document describes different biosafety levels and types of biosafety cabinets. It outlines 4 biosafety levels (BSL 1-4) with increasing containment requirements based on the risk of the pathogens handled. BSL1 requires standard practices while BSL4 requires the highest level of containment for dangerous exotic agents. It also describes 3 classes of biosafety cabinets (Class I-III). Class I provides personnel and environmental protection while Class II (types A1, A2, B1, B2) provides product, personnel and environmental protection. Class III provides the highest level of containment suitable for BSL3 and BSL4 work.
Biosafety is the application of safety precautions that reduce a Laboratory based risk of exposure to a potentially infectious material and limit contamination of the working and surrounding environment.
The primary principle of biosafety is “Containment”.
Containment
The action of keeping harmful things under control and within limits
Or
A series of safe methods for managing infectious bacteria in the laboratory.
Biosafety is the application of safety precautions that reduce a Laboratory based risk of exposure to a potentially infectious material and limit contamination of the working and surrounding environment.
The primary principle of biosafety is “Containment”.
Containment
The action of keeping harmful things under control and within limits
Or
A series of safe methods for managing infectious bacteria in the laboratory.
Safety cabinets are intended to protect a laboratory worker from aerosols and airborne particles.
They will not protect the person from spillages and the consequences of mishandling and poor technique.
Aerosol particles of less than 5 µm in diameter and small droplets of 5–100 µm in diameter are not visible to the naked eye.
The laboratory worker is generally not aware that such particles are being generated and may be inhaled or may cross contaminate work surface materials.
BSCs, when properly used, have been shown to be highly effective in reducing laboratory-acquired infections and cross-contaminations of cultures due to aerosol exposures. BSCs also protect the environment.
Most BSCs use high efficiency particulate air (HEPA) filters in the exhaust and supply systems.
The exception is a Class I BSC, which does not have HEPA filtered supply air.
Safety cabinets are intended to protect a laboratory worker from aerosols and airborne particles.
They will not protect the person from spillages and the consequences of mishandling and poor technique.
Aerosol particles of less than 5 µm in diameter and small droplets of 5–100 µm in diameter are not visible to the naked eye.
The laboratory worker is generally not aware that such particles are being generated and may be inhaled or may cross contaminate work surface materials.
BSCs, when properly used, have been shown to be highly effective in reducing laboratory-acquired infections and cross-contaminations of cultures due to aerosol exposures. BSCs also protect the environment.
Most BSCs use high efficiency particulate air (HEPA) filters in the exhaust and supply systems.
The exception is a Class I BSC, which does not have HEPA filtered supply air.
The application of knowledge, techniques and equipment to prevent a personal laboratory and environmental exposure to potentially infectious agents or biohazard is known as biosafety.
Biosafety defines the containment conditions under which infectious agents can be safely manipulated.
The objective of containment is to confine biohazard and to reduce the potential exposure of the laboratory worker, persons outside of the laboratory, and the environment to potentially infectious agents.
Workplace safety is an important aspect to protect personnel against injury or serious accident.In case of animal cell culture safety takes a front seat due to nature of work i.e. handling of human cells and tissues, viruses with high potential to cause infections to humans and other adventitious micro organisms. This presentation presents various methods of safety to protect lab personnel from infectious biological agents.
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Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
1. Biosafety levels
BSL 1:
Practices safety equipment and facilities appropriate for
work with defined and characterized strains of viable
microbes not known to cause disease in healthy adult
humans.
The laboratory is not necessarily separated from the
general building.
Work is generally conducted on open bench tops using
standard microbiological practices.
Special containments equipment or facility design is
neither required nor generally used.
Laboratory personal should have specific training in
procedure conducted.
Eating and drinking must be avoided.
Organisms- E.coli, B.subtilis, Saccharomyces cerevisiae.
BSL 2:
• Practices, safety equipment and facilities appropriate for
work done with a broad spectrum of indigenous
moderate-risk agents present in the community and
associated with human disease in varying severity.
• Differs from BSL 1 in that:
2. a) Laboratory personal have specific training in handling
pathogenic agents and/or directed by competant scientist.
b) Access to the lab is limited when work is being
conducted.
c) Extreme precautions are taken with contaminated sharp
items.
d) Certain procedures in which infectious aerosols maybe
created are conducted in BSC.
e) Class I & II BSC are used.
f) Eg. HAV, HIV, HBV, HCV, pathogenic E.coli,
staphylococcus aureus, salmonella, P. falciparum and
Toxoplasma gondii.
BSL 3:
• Practices, safety equipment and facilities appropriate for
work done with indigenous or exotic agents with a
potential for respiratory transmission which may cause
serious and potentially lethal infection.
• More emphasis is placed on primary and secondary
barriers to protect personal in the contagious area, the
community, and the environment from exposure to
potentially infecious aerosols.
• A class I or class II BSC is required for work involving
these agents.
• Eg. M. tuberculosis, Francisella tularensis, Chlamydia
psittaci, Venezuelan equine encephalitis virus, SARS
corona virus, Coxiella burnetti, Ricketsia rickettsi.
3. BSL 4:
• Practices, safety equipment and facilities appropriate for
work done with dangerous and exotic agents which pose
a high risk of life threatening disease.
• Materials leaving the cabinet must be decontaminated
and the cabinet must also be disinfected.
• Workers must pass through a chemical shower.
• Entry is strictly restricted.
• The entrance is air locked.
• Eg. Viruses that cause viral haemorrhagic fever, lassa
virus, crimean-congo haemorrahagic fever. Pathogens
like hendra virus, nipoh virus, flavi virus, variola virus.
4. Biosafety cabinets
Biosafety cabinets are used as the primary means of
containment for working safely with infectious
microorganisms.
Biosafety cabinets are designed to prevent biological exposure
to personnel and the environment and may also protect
experimental material from being contaminated when
appropriate practices and procedures are followed.
Biological safety cabinets use high efficiency particulate air
(HEPA) filters in their exhaust and/or supply systems. These
filtered cabinets are primarily designed to protect against
exposure to particulates, including biological agents used in
the cabinet.
The Class I Biosafety Cabinet
Provides personnel and environmental protection, but no
product protection.
It has a negative pressure ventilated cabinet.
It has similar turbulent air flow as a chemical fume hood,
but has a HEPA filter in the exhaust system to provide
containment and environmental protection with front
opening.
This older class of biosafety cabinet is rarely seen in
MUSC's biomedical and microbiological laboratories.
Low and moderate risk agents can be handled using this
type.
All the air from the cabinet is exhausted through HEPA
filter into the lab or outside
5. The Class II Biosafety Cabinet
A1 type:
It is designed with inward air flow to provide protection
to the user, the experimental material and the
environment.
The Class II biological safety cabinet is the type most
commonly used in MUSC's biomedical and
microbiological laboratories.
It provides product, environmental and personal
protection.
The air is mostly filtered.
All the air from the cabinet is exhausted through HEPA
filter into the lab or outside
It has a positive pressured plenum
No toxic chemical is handled in this type.
70% of the air is recirculated and 30% of the air is
exhausted through HEPA filter.
A2 type:
It has both positive and negative pressured plenum. In
case of the air leaking out of positive pressured plenum,
it is trapped by negative pressured plenum.
70% of the air is recirculated and 30% of the air is
exhausted through HEPA filter.
Exhaust air is hard ducted.
B1 type:
30% of the air is recirculated and 70% of the air is
exhausted through HEPA filter.
Products of BSL 2 and 3 are used in this type.
6. The cabinet exhaust system is connected to the building
exhaust system and the air is ventilated out.
B2 type:
There is no recirculation in this type and so 100% of the
air is exhausted.
Radio nucleotides and toxic chemicals can be handled in
this type.
Exhaust hard ducted out.
Products of BSL 2 and 3 are used in this type.
The Class III Biosafety Cabinet
The Class III biological safety cabinet is most suitable
for work with biohazardous agents requiring high contain
(biosafety level 3 or 4).
The Class III cabinet is completely enclosed, HEPA
filter-ventilated cabinet fitted with glove ports and
decontamination capabilities for entry and exit of
material.
It offers the highest degree of personnel and
environmental protection from infectious aerosols.