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Biosafety LeVeLs &Biosafety LeVeLs &
Biosafety CaBinetsBiosafety CaBinets
 Bio safety Levels
 Air Filtration in Biosafety cabinets
 Standards
 Hood , Laminar flow & Bio safety Cabinets
 Classification –Bio safety Cabinets
TopicsTopics
Biosafety LevelsBiosafety Levels
 Bio Safety Level (BSL)-I:
Well-characterized agents not known to consistently cause disease in healthy adult
humans, and of minimal potential hazard to laboratory personnel and the
Environment.
Standard practices required:
 frequent hand washing
 door that can be kept closed when working
 limits on access to the lab space when working
 no eating, drinking, storage of food in laboratory
 care to minimize splashes and actions that may create aerosols (tiny droplets)
 decontamination of work surfaces after every use
Primary Safety Barriers- Not Required
Biosafety LevelsBiosafety Levels

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The document discusses the history and development of biosafety levels for handling biological agents. It notes that the first biosafety cabinet was created in 1943 and that the CDC began specifying biosafety levels in 1964, establishing levels 1 through 4. It provides details on the standard practices, safety equipment, and facilities required for biosafety levels 1 through 4, with level 1 requiring the fewest containment procedures and level 4 the most stringent for dangerous infectious agents.

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Biosafety in microbiology laboratory
Biosafety  in  microbiology laboratoryBiosafety  in  microbiology laboratory
Biosafety in microbiology laboratory

This document discusses biosafety in microbiology laboratories. It describes the different biosafety levels from 1 to 4, with level 1 requiring the fewest precautions for agents that do not consistently cause disease, and level 4 requiring the strictest methods for dealing with highly dangerous agents. For each level, it outlines the standard practices, personal protective equipment, facility requirements and examples of agents worked with at that level. Biosafety levels help ensure the safe handling of pathogens to protect laboratory workers from acquiring infections.

 Bio Safety Level (BSL)-II:
• Suitable for work involving agents of moderate potential hazard to personnel
and the environment.
• Generally associated with human disease
Safety Equipment (Primary barriers):
• Class I or II Bio safety Cabinets or other physical containment devices (eg….
Aerosol tight lid in Centrifuges) are used
• Personnel Protection Equipment: Laboratory coats, gloves, face protection as
needed
Facilities (Secondary barriers):
• Provide lockable doors for facilities that house restricted agents
• Locating new laboratories away from public areas.
• Furniture used in laboratory work should be covered with a non-fabric material that
can be easily be decontaminated.
• An eye wash station is readily available.
Biosafety LevelsBiosafety Levels
 Bio Safety Level (BSL)-III:
Applicable where work is done with indigenous or exotic agents which may cause
serious or potentially lethal disease as a result of exposure by the inhalation
route.
Safety Equipment (Primary barriers):
• Class I or II Bio safety Cabinets
• Personnel Protection Equipment: Laboratory coats; gloves; face protection as
needed
• Facilities (Secondary barriers):
• The laboratory has special engineering and design feature . The exhaust air from the
laboratory room is discharged to the outdoors
• The ventilation to the laboratory is balanced to provide directional airflow into the
room
Biosafety LevelsBiosafety Levels
 Bio Safety Level (BSL)-IV:
Dangerous/exotic agents which pose high risk of life-threatening disease, aerosol-
transmitted lab infections or related agents with unknown risk of transmission.
Safety Equipment (Primary barriers):
All procedures conducted in Class III Bio safety Cabinets
or Class I or II Bio Safety Cabinets in combination with full-body, air-supplied, positive
pressure personnel suit.
Facilities (Secondary barriers):
BSL-3 plus:
• Separate building or isolated zone
• Dedicated supply and exhaust and decontamination systems
Biosafety LevelsBiosafety Levels
Air Filtration in Biosafety cabinetsAir Filtration in Biosafety cabinets

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The document discusses primary containment and other biological hazards. It outlines a hierarchy of controls to prevent exposure to biological hazards, including engineering controls, administrative controls, and personal protective equipment (PPE). Primary containment equipment like biosafety cabinets (BSCs) and centrifuges contain hazards at the source. Secondary containment provides barriers around primary containment through facilities and room design. Tertiary containment establishes barriers beyond containment laboratories. PPE like gloves, gowns, and respirators are used to protect workers. Proper use and decontamination of equipment minimizes exposure risks from biological hazards in laboratories.

biosafety
Bio-safety Cabinet
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Lecture 3: biosafety levels
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The document discusses biosafety levels (BSL) which are used to classify biological agents based on risk. There are four biosafety levels, with BSL-1 posing the lowest risk (ex. E. coli bacteria) and BSL-4 posing the highest risk (ex. Ebola virus). Each level has specific containment controls for laboratory practices, safety equipment, and facility construction required to safely work with the biological agents in that risk group. The summary outlines some of the key containment controls like personal protective equipment, biological safety cabinets, and facility access restrictions that distinguish the different biosafety levels.

•High efficiency particulate air (HEPA) filters
•Ultra low particulate air (ULPA) filters
Air Filtration in Biosafety cabinetsAir Filtration in Biosafety cabinets
Variations in HEPA
filter efficiency from
99.95 % to 99.99 % are
usually due to
manufacturing
techniques.
Depending upon its quality, a HEPA
filter is able to trap from 9,995 to 9,999
of every 10,000 particles
An ULPA filter is able to
trap from 99,995 to 99,999 of
every 100,000 particles
ULPA - this is at least 10 times
better than HEPA filtration !!!
International Standards on Biosafety CabinetInternational Standards on Biosafety Cabinet
Why a standard ?Why a standard ?
International Standards on Biosafety CabinetInternational Standards on Biosafety Cabinet
International Standards are mainly issued by two Organisations:
International Standard Organisation (ISO), based in Geneva
(Switzerland),
Comitee Europeen de Normalisation (CEN) based in Brussels
(Belgium).
These standards replace all other local standards on the same
subject.
The aim of the standards is to construct a mechanism to provide
manufacturer and user of a common site to specify technical
specifications and features.
Both construction and testing of the cabinet are covered

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This document discusses different types of biosafety cabinets and compares them to laminar air flow cabinets. It defines biosafety cabinets as enclosed, ventilated workspaces for safely handling potentially hazardous biological materials. There are several classes of biosafety cabinets that provide different levels of protection for personnel, products, and the environment. Class I cabinets protect personnel and the environment but not products, while Class II and III cabinets protect all three through inward air flow and HEPA filtration of exhaust. The key differences between biosafety cabinet classes and laminar air flow cabinets are also reviewed.

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Biosafety - History, Levels and issues
Biosafety - History, Levels and issuesBiosafety - History, Levels and issues
Biosafety - History, Levels and issues

This document provides an introduction to biosafety. It defines biosafety as safety from exposure to infectious agents according to the CDC. It then discusses the history of biosafety, including the first biosafety conference in 1955 and the establishment of biosafety levels 1 through 4 by the CDC. The document outlines the need for biosafety in laboratories working with infectious agents and describes the scope of biosafety across fields like medicine, agriculture and exobiology. It also covers biosafety issues in academic research, regulations, signage, hazardous materials, and provides details on the four biosafety levels based on pathogen risk.

Clinical Laboratory Biosafety
Clinical Laboratory BiosafetyClinical Laboratory Biosafety
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The application of knowledge, techniques and equipment to prevent a personal laboratory and environmental exposure to potentially infectious agents or biohazard is known as biosafety. Biosafety defines the containment conditions under which infectious agents can be safely manipulated. The objective of containment is to confine biohazard and to reduce the potential exposure of the laboratory worker, persons outside of the laboratory, and the environment to potentially infectious agents.

biosafety levelslaboratory biosafetyrisk group organisms handling in the laboratory
International Standards on Biosafety CabinetInternational Standards on Biosafety Cabinet
Standards:
BS-5726 (U.K.)
NF X44-201 (France)
DIN 12950 (Germany)
Now Replaced by EN 12469
EN 12469
European Standards on Design, Construction & Testing of Bio safety Cabinets
In addition to comply DIN 12980 for safety handling of cytotoxic drugs
NSF-49 (National Sanitation Foundation)
In the USA the NSF-49, was one of the first standards on this subject and was the
reference point for a lot of manufacturers. NSF is an independent organisation that
works as neutral agency to help on the problems that could affect to public health
and environment protection.
Was published in 1976.
Difference between Hood and Safety CabinetDifference between Hood and Safety Cabinet
Exhaust of
vapors and
aerosols from
Laboratory to
outside
No validatable
product or
personnel
protection
Validatable
personnel, product
& environmental
protection
Filters and
blower(s)
incorporated
HoodHood Safety CabinetSafety Cabinet
A fume hood is a ventilation device that is designed to
limit exposure to hazardous fumes vapors or dust. A fume
hood is typically a large piece of equipment enclosing
five sides of a work area, the bottom of which is most
commonly located at a standing work height.
Two main types exist, ducted and recalculating. The
principle is the same for both types: air is drawn in from
the front (open) side of the cabinet, and either expelled
outside the building or made safe through filtration and
fed back into the room.
Fume HoodFume Hood
Fume Hood
Only for Product Protection
No Personnel or Environment Protection
Laminar FlowLaminar Flow
A. front opening
B. sash
C. supply HEPA filter
D. blower
Vertical Laminar Flow

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Biosafety levels range from 1 to 4 based on the hazards posed by infectious agents, with level 1 posing minimal risk and level 4 the highest. Biosafety level 1 involves standard precautions for microbes not known to cause disease in healthy adults. Level 2 requires restricted access and personal protective equipment for work with moderate hazards. Level 3 involves serious diseases transmitted through respiratory routes and requires medical surveillance, immunizations, respirators and controlled lab access. Biosafety level 4 is the highest level involving dangerous exotic microbes and requires change of clothes, showering and separate containment facilities. Adherence to biosafety guidelines and regulations helps reduce laboratory risks.

Laminar FlowLaminar Flow
A. front opening
B. supply grille
C. supply HEPA filter
D. supply plenum
E. blower
F. Grille
Horizontal Laminar Flow
Classification of Bio Safety CabinetClassification of Bio Safety Cabinet
Biological Safety Cabinet
Class I Class II
Class
III
Type A Type B
Type A
I
Type A
II
Type B
I
Type B
II
Classification of Biosafety CabinetClassification of Biosafety Cabinet
Class I:
Personnel and Environment protection.
Product is not protected.
Class II:
Personnel, Product and Environment are protected.
Class III:
Maximum protection to Personnel, Product and Environment.
Classification of Biosafety CabinetClassification of Biosafety Cabinet
A. Front opening
B. Sash
C. Exhaust HEPA
Filter
D. Exhaust plenum
Class I Biosafety Cabinets
 This is a negative-pressure,
ventilated cabinet usually
operated with an open front.
 All of the air from the cabinet is
exhausted through a HEPA filter
either into the laboratory or to
the outside.
 The Class I BSC is designed for
general microbiological research
with low- and moderate-risk
agents.

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Biosafety Level 4
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Biosafety level 4 requires the highest level of containment precautions and is required for work with dangerous and exotic agents that can cause severe or fatal disease for which there are no vaccines or treatments. Strict protocols must be followed, including requiring two people to work at all times, full protective clothing and respiratory equipment, and decontamination of all materials. The laboratory facilities for biosafety level 4 include primary containment like class III cabinets and sealed suit laboratories, emergency power, controlled access and air systems, and sterilization of all waste before disposal.

Classification of Biosafety CabinetClassification of Biosafety Cabinet
A. front opening
B. sash
C. exhaust HEPA filter
D. rear plenum
E. supply HEPA filter
F. blower
Class II Type A-I Biosafety Cabinets
 It is designed with inward air
flow to protect personnel,
Product & Environment
 HEPA-filtered downward
vertical laminar airflow for
product protection, and HEPA-
filtered exhaust air for
environmental protection
Classification of Biosafety CabinetClassification of Biosafety Cabinet
 Face Velocity 75 fpm
 70% re circulated through HEPA Filter
 30% Exhausted Through HEPA Filter
 Cannot be used for Radio Nucleotides and Toxic Chemicals
 Biosafety Levels 2 & 3
 User, Product, & Environment Protection
Class II Type A-I Biosafety Cabinets
Classification of Biosafety CabinetClassification of Biosafety Cabinet
Class II Type A-II Biosafety Cabinets
A. front opening
B. sash
C. exhaust HEPA filter
D. supply HEPA filter
E. positive pressure plenum
F. negative pressure plenum
Note: The cabinet exhaust
needs to be connected to the
building exhaust system.
Classification of Biosafety CabinetClassification of Biosafety Cabinet
Class II Type A-II Biosafety Cabinets
 Downward Velocity 100 fpm
 70% re circulated through HEPA
 Filter30% Exhausted Through HEPA Filter
 Can be used for Radio Nucleotides and Toxic Chemicals
 Biosafety Levels 2 & 3User, Product, & Environment Protection
 Under Negative Pressure to room
 Exhaust Air is Hard Ducted

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The document describes different biosafety levels and types of biosafety cabinets. It outlines 4 biosafety levels (BSL 1-4) with increasing containment requirements based on the risk of the pathogens handled. BSL1 requires standard practices while BSL4 requires the highest level of containment for dangerous exotic agents. It also describes 3 classes of biosafety cabinets (Class I-III). Class I provides personnel and environmental protection while Class II (types A1, A2, B1, B2) provides product, personnel and environmental protection. Class III provides the highest level of containment suitable for BSL3 and BSL4 work.

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Classification of Biosafety CabinetClassification of Biosafety Cabinet
Class II Type B-I Biosafety Cabinets
A. front opening
B. Sash
C. exhaust HEPA filter
D. supply plenum
E. supply HEPA filter
F. Blower
G. negative pressure exhaust plenum
Note: The cabinet exhaust needs to be
connected to the building exhaust
system.
Classification of Biosafety CabinetClassification of Biosafety Cabinet
Class II Type B-I Biosafety Cabinets
 Downward Velocity 100 fpm
 30% re circulated through HEPA Filter
 70% Exhausted Through HEPA Filter
 Can be used for Radio Nucleotides and Toxic Chemicals
(Low Levels / Volatility)
 Biosafety Levels 2 & 3User, Product, & Environment
Protection
Classification of Biosafety CabinetClassification of Biosafety Cabinet
Class II Type B-II Biosafety Cabinets
A. front opening
B. Sash
C. exhaust HEPA filter
D. supply HEPA filter
E. negative pressure exhaust plenum
F. supply blower
G. filter screen
Note: The carbon filter in the building
exhaust is not shown. The cabinet
exhaust needs to be connected to
the building exhaust system
Classification of Biosafety CabinetClassification of Biosafety Cabinet
Class II Type B-II Biosafety Cabinets
 Downward Velocity 100 fpm
 No Re-circulation100% Exhausted Through HEPA Filter
 Can be used for Radio Nucleotides and Toxic Chemicals
 Biosafety Levels 2 & 3User, Product, & Environment
Protection
 Exhaust HEPA Filtered
 Exhaust Hard Ducted out.

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The document discusses the four classes of biological safety cabinets (BSCs). It provides details on each class: Class I BSCs provide personnel and environmental protection but no product protection. Airflow is inward and exhausted through a HEPA filter. Class II BSCs provide personnel, environmental and product protection. They maintain laminar airflow and exhaust air through HEPA filters, with options for recirculation or external exhaust. Class III BSCs are fully enclosed and gas-tight, providing maximum containment. All supply and exhaust air is HEPA filtered before being externally exhausted under negative pressure. Access is through dunk tanks or double-door pass-through boxes.

Bio safety (1).pptx
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This document discusses biosafety in microbiology laboratories. It defines biosafety and explains why it is important to protect laboratory workers from infection. The document outlines standard biosafety practices like restricting access, hand washing, and personal protective equipment. It describes various routes of infection and lists pathogens of special concern. Different biosafety levels and containment procedures are discussed based on the hazard group of pathogens. The roles of engineering controls, standard practices and biosafety cabinets are explained.

biosafetycabinetsbiosafety levels
Biosafety
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Biosafety

This document discusses biosafety levels for working with microorganisms. It describes the four biosafety levels from BSL-1 to BSL-4, providing more stringent safety protocols for higher levels that involve more dangerous pathogens. BSL-1 involves well-characterized microbes and requires standard practices like handwashing. BSL-2 adds personal protective equipment, waste decontamination, and controlled access. BSL-3 further restricts access and requires respiratory protection due to airborne risks. BSL-4 is for dangerous exotic pathogens and requires the most stringent containment and protective equipment, including full body isolation suits.

Classification of Biosafety CabinetClassification of Biosafety Cabinet
Class III Biosafety Cabinets
A. glove ports with O-ring for attaching
arm-length gloves to cabinet
B. Sash
C. exhaust HEPA filter
D. supply HEPA filter
E. double-ended autoclave or pass-
through box
Note: A chemical dunk tank may be
installed which would be located
beneath the work surface of the BSC
with access from above. The cabinet
exhaust needs to be connected to the
building exhaust system.
Classification of Biosafety CabinetClassification of Biosafety Cabinet
Class III Biosafety Cabinets
 This is a totally enclosed, ventilated cabinet
 Gas-tight construction
 Offers the highest degree of personnel and environmental protection
from infectious aerosols as well as protection of research materials
from microbiological contaminants.
Cytotoxic Drug Safety CabinetsCytotoxic Drug Safety Cabinets
Hepa filter exhaust #2
Hepa filter (pre-filter) #1
Laminar flow filter
Carbon filter exhaust #3 (option)
Dedicated exhaust flow
blower
All contaminated areas under
NEGATIVE pressure
Dedicated Laminar flow
blower
70% re circulated HEPA
filtered laminar flow
Provides sample
protection
30% inflow provides operator
Safety barrier
Can you see the problems ?Can you see the problems ?
Window
Air vent
Ceiling clearance
or wall clearance
Front
Venting
Exhaust
BEWAR
E

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2018 biosafety-shaghlil ahmad, jennifer nammoura, taha ali, massry ali
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The document discusses biological safety cabinets, which are enclosed ventilated workspaces that protect operators, the laboratory environment, and work materials from exposure to infectious aerosols and splashes. Biological safety cabinets are classified into three classes based on their level of protection and designated biosafety level. Class I cabinets provide protection for the environment but not products, Class II cabinets provide protection for personnel, environment, and products through HEPA filtration of inflow and downflow air, and Class III cabinets provide the highest level of containment and filtration for work with hazardous pathogens. Selection of the appropriate biological safety cabinet depends on the needed type of protection against various risk groups of microorganisms or other hazards such as radionuclides or toxic chemicals

Laminar Flow Hoods Biological Safety Cabinets.pdf
Laminar Flow Hoods  Biological Safety Cabinets.pdfLaminar Flow Hoods  Biological Safety Cabinets.pdf
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need

biosafety
Lab. operations
Lab. operationsLab. operations
Lab. operations

The document provides guidelines for designing and operating a microbiology laboratory. It discusses objectives for laboratory design which are to provide a safe environment for personnel and allow flexibility for research and teaching while anticipating health and safety hazards. Standards are outlined for containment of hazardous biological agents classified into four groups. Considerations for laboratory design include building design, internal structure and services, work flow, equipment used, and operation practices like documentation, standard operating procedures, and ensuring aseptic techniques.

Location In LabLocation In Lab
Location A, E and F
are well selected.
As well influencing
air flows through
the Laboratory
personnel traffic is
avoided.
Location B is often
influenced from air
flows in the Room.
Location C is
influenced from
personnel traffic
and Room air
flows.
Location D is also very often influenced
from personnel traffic and air flows.
Safety Cabinets should only be
placed in quiet areas!
Only validated tests can show placement in the
laboratory is correct.
In this instance the tests are invaluable!
THANK YOUTHANK YOU

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Biosafety level and biosafety cabinets

  • 1. Biosafety LeVeLs &Biosafety LeVeLs & Biosafety CaBinetsBiosafety CaBinets
  • 2.  Bio safety Levels  Air Filtration in Biosafety cabinets  Standards  Hood , Laminar flow & Bio safety Cabinets  Classification –Bio safety Cabinets TopicsTopics
  • 4.  Bio Safety Level (BSL)-I: Well-characterized agents not known to consistently cause disease in healthy adult humans, and of minimal potential hazard to laboratory personnel and the Environment. Standard practices required:  frequent hand washing  door that can be kept closed when working  limits on access to the lab space when working  no eating, drinking, storage of food in laboratory  care to minimize splashes and actions that may create aerosols (tiny droplets)  decontamination of work surfaces after every use Primary Safety Barriers- Not Required Biosafety LevelsBiosafety Levels
  • 5.  Bio Safety Level (BSL)-II: • Suitable for work involving agents of moderate potential hazard to personnel and the environment. • Generally associated with human disease Safety Equipment (Primary barriers): • Class I or II Bio safety Cabinets or other physical containment devices (eg…. Aerosol tight lid in Centrifuges) are used • Personnel Protection Equipment: Laboratory coats, gloves, face protection as needed Facilities (Secondary barriers): • Provide lockable doors for facilities that house restricted agents • Locating new laboratories away from public areas. • Furniture used in laboratory work should be covered with a non-fabric material that can be easily be decontaminated. • An eye wash station is readily available. Biosafety LevelsBiosafety Levels
  • 6.  Bio Safety Level (BSL)-III: Applicable where work is done with indigenous or exotic agents which may cause serious or potentially lethal disease as a result of exposure by the inhalation route. Safety Equipment (Primary barriers): • Class I or II Bio safety Cabinets • Personnel Protection Equipment: Laboratory coats; gloves; face protection as needed • Facilities (Secondary barriers): • The laboratory has special engineering and design feature . The exhaust air from the laboratory room is discharged to the outdoors • The ventilation to the laboratory is balanced to provide directional airflow into the room Biosafety LevelsBiosafety Levels
  • 7.  Bio Safety Level (BSL)-IV: Dangerous/exotic agents which pose high risk of life-threatening disease, aerosol- transmitted lab infections or related agents with unknown risk of transmission. Safety Equipment (Primary barriers): All procedures conducted in Class III Bio safety Cabinets or Class I or II Bio Safety Cabinets in combination with full-body, air-supplied, positive pressure personnel suit. Facilities (Secondary barriers): BSL-3 plus: • Separate building or isolated zone • Dedicated supply and exhaust and decontamination systems Biosafety LevelsBiosafety Levels
  • 8. Air Filtration in Biosafety cabinetsAir Filtration in Biosafety cabinets
  • 9. •High efficiency particulate air (HEPA) filters •Ultra low particulate air (ULPA) filters Air Filtration in Biosafety cabinetsAir Filtration in Biosafety cabinets Variations in HEPA filter efficiency from 99.95 % to 99.99 % are usually due to manufacturing techniques. Depending upon its quality, a HEPA filter is able to trap from 9,995 to 9,999 of every 10,000 particles An ULPA filter is able to trap from 99,995 to 99,999 of every 100,000 particles ULPA - this is at least 10 times better than HEPA filtration !!!
  • 10. International Standards on Biosafety CabinetInternational Standards on Biosafety Cabinet
  • 11. Why a standard ?Why a standard ?
  • 12. International Standards on Biosafety CabinetInternational Standards on Biosafety Cabinet International Standards are mainly issued by two Organisations: International Standard Organisation (ISO), based in Geneva (Switzerland), Comitee Europeen de Normalisation (CEN) based in Brussels (Belgium). These standards replace all other local standards on the same subject. The aim of the standards is to construct a mechanism to provide manufacturer and user of a common site to specify technical specifications and features. Both construction and testing of the cabinet are covered
  • 13. International Standards on Biosafety CabinetInternational Standards on Biosafety Cabinet Standards: BS-5726 (U.K.) NF X44-201 (France) DIN 12950 (Germany) Now Replaced by EN 12469 EN 12469 European Standards on Design, Construction & Testing of Bio safety Cabinets In addition to comply DIN 12980 for safety handling of cytotoxic drugs NSF-49 (National Sanitation Foundation) In the USA the NSF-49, was one of the first standards on this subject and was the reference point for a lot of manufacturers. NSF is an independent organisation that works as neutral agency to help on the problems that could affect to public health and environment protection. Was published in 1976.
  • 14. Difference between Hood and Safety CabinetDifference between Hood and Safety Cabinet Exhaust of vapors and aerosols from Laboratory to outside No validatable product or personnel protection Validatable personnel, product & environmental protection Filters and blower(s) incorporated HoodHood Safety CabinetSafety Cabinet
  • 15. A fume hood is a ventilation device that is designed to limit exposure to hazardous fumes vapors or dust. A fume hood is typically a large piece of equipment enclosing five sides of a work area, the bottom of which is most commonly located at a standing work height. Two main types exist, ducted and recalculating. The principle is the same for both types: air is drawn in from the front (open) side of the cabinet, and either expelled outside the building or made safe through filtration and fed back into the room. Fume HoodFume Hood Fume Hood
  • 16. Only for Product Protection No Personnel or Environment Protection Laminar FlowLaminar Flow A. front opening B. sash C. supply HEPA filter D. blower Vertical Laminar Flow
  • 17. Laminar FlowLaminar Flow A. front opening B. supply grille C. supply HEPA filter D. supply plenum E. blower F. Grille Horizontal Laminar Flow
  • 18. Classification of Bio Safety CabinetClassification of Bio Safety Cabinet Biological Safety Cabinet Class I Class II Class III Type A Type B Type A I Type A II Type B I Type B II
  • 19. Classification of Biosafety CabinetClassification of Biosafety Cabinet Class I: Personnel and Environment protection. Product is not protected. Class II: Personnel, Product and Environment are protected. Class III: Maximum protection to Personnel, Product and Environment.
  • 20. Classification of Biosafety CabinetClassification of Biosafety Cabinet A. Front opening B. Sash C. Exhaust HEPA Filter D. Exhaust plenum Class I Biosafety Cabinets  This is a negative-pressure, ventilated cabinet usually operated with an open front.  All of the air from the cabinet is exhausted through a HEPA filter either into the laboratory or to the outside.  The Class I BSC is designed for general microbiological research with low- and moderate-risk agents.
  • 21. Classification of Biosafety CabinetClassification of Biosafety Cabinet A. front opening B. sash C. exhaust HEPA filter D. rear plenum E. supply HEPA filter F. blower Class II Type A-I Biosafety Cabinets  It is designed with inward air flow to protect personnel, Product & Environment  HEPA-filtered downward vertical laminar airflow for product protection, and HEPA- filtered exhaust air for environmental protection
  • 22. Classification of Biosafety CabinetClassification of Biosafety Cabinet  Face Velocity 75 fpm  70% re circulated through HEPA Filter  30% Exhausted Through HEPA Filter  Cannot be used for Radio Nucleotides and Toxic Chemicals  Biosafety Levels 2 & 3  User, Product, & Environment Protection Class II Type A-I Biosafety Cabinets
  • 23. Classification of Biosafety CabinetClassification of Biosafety Cabinet Class II Type A-II Biosafety Cabinets A. front opening B. sash C. exhaust HEPA filter D. supply HEPA filter E. positive pressure plenum F. negative pressure plenum Note: The cabinet exhaust needs to be connected to the building exhaust system.
  • 24. Classification of Biosafety CabinetClassification of Biosafety Cabinet Class II Type A-II Biosafety Cabinets  Downward Velocity 100 fpm  70% re circulated through HEPA  Filter30% Exhausted Through HEPA Filter  Can be used for Radio Nucleotides and Toxic Chemicals  Biosafety Levels 2 & 3User, Product, & Environment Protection  Under Negative Pressure to room  Exhaust Air is Hard Ducted
  • 25. Classification of Biosafety CabinetClassification of Biosafety Cabinet Class II Type B-I Biosafety Cabinets A. front opening B. Sash C. exhaust HEPA filter D. supply plenum E. supply HEPA filter F. Blower G. negative pressure exhaust plenum Note: The cabinet exhaust needs to be connected to the building exhaust system.
  • 26. Classification of Biosafety CabinetClassification of Biosafety Cabinet Class II Type B-I Biosafety Cabinets  Downward Velocity 100 fpm  30% re circulated through HEPA Filter  70% Exhausted Through HEPA Filter  Can be used for Radio Nucleotides and Toxic Chemicals (Low Levels / Volatility)  Biosafety Levels 2 & 3User, Product, & Environment Protection
  • 27. Classification of Biosafety CabinetClassification of Biosafety Cabinet Class II Type B-II Biosafety Cabinets A. front opening B. Sash C. exhaust HEPA filter D. supply HEPA filter E. negative pressure exhaust plenum F. supply blower G. filter screen Note: The carbon filter in the building exhaust is not shown. The cabinet exhaust needs to be connected to the building exhaust system
  • 28. Classification of Biosafety CabinetClassification of Biosafety Cabinet Class II Type B-II Biosafety Cabinets  Downward Velocity 100 fpm  No Re-circulation100% Exhausted Through HEPA Filter  Can be used for Radio Nucleotides and Toxic Chemicals  Biosafety Levels 2 & 3User, Product, & Environment Protection  Exhaust HEPA Filtered  Exhaust Hard Ducted out.
  • 29. Classification of Biosafety CabinetClassification of Biosafety Cabinet Class III Biosafety Cabinets A. glove ports with O-ring for attaching arm-length gloves to cabinet B. Sash C. exhaust HEPA filter D. supply HEPA filter E. double-ended autoclave or pass- through box Note: A chemical dunk tank may be installed which would be located beneath the work surface of the BSC with access from above. The cabinet exhaust needs to be connected to the building exhaust system.
  • 30. Classification of Biosafety CabinetClassification of Biosafety Cabinet Class III Biosafety Cabinets  This is a totally enclosed, ventilated cabinet  Gas-tight construction  Offers the highest degree of personnel and environmental protection from infectious aerosols as well as protection of research materials from microbiological contaminants.
  • 31. Cytotoxic Drug Safety CabinetsCytotoxic Drug Safety Cabinets Hepa filter exhaust #2 Hepa filter (pre-filter) #1 Laminar flow filter Carbon filter exhaust #3 (option) Dedicated exhaust flow blower All contaminated areas under NEGATIVE pressure Dedicated Laminar flow blower 70% re circulated HEPA filtered laminar flow Provides sample protection 30% inflow provides operator Safety barrier
  • 32. Can you see the problems ?Can you see the problems ? Window Air vent Ceiling clearance or wall clearance Front Venting Exhaust BEWAR E
  • 33. Location In LabLocation In Lab Location A, E and F are well selected. As well influencing air flows through the Laboratory personnel traffic is avoided. Location B is often influenced from air flows in the Room. Location C is influenced from personnel traffic and Room air flows. Location D is also very often influenced from personnel traffic and air flows. Safety Cabinets should only be placed in quiet areas! Only validated tests can show placement in the laboratory is correct. In this instance the tests are invaluable!