3. Learning Outcomes On successful completion of the course, the participant will be able to: Discuss the components of the BMBL 5th edition Discuss characteristics of BSL- 1, BSL-2, BSL-3, and BSL-4 laboratories Discuss and demonstrate standard laboratory practices as applied in BSL- 1, BSL-2, BSL-3, and BSL-4 laboratories
4. What Do You Think? Is the laboratory a hazardous work environment? 2. Do you work with hazardous materials, i.e. biological, chemical? Then, why do people choose to work without gloves, safety glasses, buttoned lab coats etc. in the laboratory?
51. CDC/NIH Guidelines Biohazard An agent of biological origin that has the capacity to produce deleterious effects on humans, i.e. microorganisms, toxins and allergens derived from those organisms; and allergens and toxins derived from higher plants and animals.
52. CDC/NIH Guidelines Biohazard Classification: Biosafety Levels BSL1 Agents not known to cause disease BSL2 Agents associated with human disease BSL3 Indigenous/exotic agents associated with human disease and with potential for aerosol transmission BSL4 Dangerous/exotic agents of life threatening nature
53. CDC/NIH Guidelines Biosafety The application of combinations of laboratory practice and procedure, laboratory facilities, and safety equipment when working with potentially infectious microorganisms.
54. CDC/NIH Guidelines Laboratory Classification: Biosafety Levels Combinations of laboratory practice and procedure, laboratory facilities, and safety equipment to provide containment Low Containment Biosafety Level 1 Biosafety Level 2 Biosafety Level 3 Biosafety Level 4 High Containment
103. Animal Facilities ABSL 1-4 Standard practices Special practices Safety equipment Facilities
104. Standard Lab Practices Standard Practices? Those practices which you should perform whenever you enter/exit the lab/animal area and during your work while in the lab/animal area.
118. Exercise #2 Jason was working alone on Sunday with Neisseriameningitidis, concentrating it with a bench-top centrifuge prior to aliquoting it into cryovials. Several days later, he developed a high fever and chills, reported to the ER, and was admitted. He lapsed into a coma and came within two hours of dying from an acute case of meningitis. Jason was working alone on Sunday with Neisseriameningitidis, concentrating it with a bench-top centrifuge prior to aliquotingit into cryovials. Several days later, he developed a high fever and chills, reported to the ER, and was admitted. He lapsed into a coma and came within two hours of dying from an acute case of meningitis.
128. Class I Class III Class II Class II Type A1 Class II Type A2 Class II Type B2 Class II Type B1 Aerosol/fume Containment Devices Biological Safety Cabinet Fume Hood
130. Biological Safety Cabinets Class I Single pass air Worker protection; no product protection Exhausts to outside (w/wo HEPA filter) Class II Re-circulated air Worker/product/environmental protection Laminar flow Exhausts inside/outside (w/wo HEPA filter) Suitable for work with BSL2-4 agents Class III Totally enclosed; air-tight Exhausted outside thru HEPA filters Suitable for work with BSL3/4 agents
