The document discusses primary containment and other biological hazards. It outlines a hierarchy of controls to prevent exposure to biological hazards, including engineering controls, administrative controls, and personal protective equipment (PPE). Primary containment equipment like biosafety cabinets (BSCs) and centrifuges contain hazards at the source. Secondary containment provides barriers around primary containment through facilities and room design. Tertiary containment establishes barriers beyond containment laboratories. PPE like gloves, gowns, and respirators are used to protect workers. Proper use and decontamination of equipment minimizes exposure risks from biological hazards in laboratories.
Biosafety is the application of safety precautions that reduce a Laboratory based risk of exposure to a potentially infectious material and limit contamination of the working and surrounding environment.
The primary principle of biosafety is “Containment”.
Containment
The action of keeping harmful things under control and within limits
Or
A series of safe methods for managing infectious bacteria in the laboratory.
Biosafety is the application of safety precautions that reduce a Laboratory based risk of exposure to a potentially infectious material and limit contamination of the working and surrounding environment.
The primary principle of biosafety is “Containment”.
Containment
The action of keeping harmful things under control and within limits
Or
A series of safe methods for managing infectious bacteria in the laboratory.
Workplace safety is an important aspect to protect personnel against injury or serious accident.In case of animal cell culture safety takes a front seat due to nature of work i.e. handling of human cells and tissues, viruses with high potential to cause infections to humans and other adventitious micro organisms. This presentation presents various methods of safety to protect lab personnel from infectious biological agents.
Basics of BioSafety
This lesson will define and present information on
methods used to provide biosafety in facilities
where potentially infectious agents are used.
These include:
Containment
Biological safety cabinets
Personal protection equipment
The facility as barrier
Secondary barriers
Safety cabinets are intended to protect a laboratory worker from aerosols and airborne particles.
They will not protect the person from spillages and the consequences of mishandling and poor technique.
Aerosol particles of less than 5 µm in diameter and small droplets of 5–100 µm in diameter are not visible to the naked eye.
The laboratory worker is generally not aware that such particles are being generated and may be inhaled or may cross contaminate work surface materials.
BSCs, when properly used, have been shown to be highly effective in reducing laboratory-acquired infections and cross-contaminations of cultures due to aerosol exposures. BSCs also protect the environment.
Most BSCs use high efficiency particulate air (HEPA) filters in the exhaust and supply systems.
The exception is a Class I BSC, which does not have HEPA filtered supply air.
The document provides a detailed overview on the basic principles of operating a biotech or micro laboratory along with basic techniques with which to handle organisms, chemicals &equipment and ensuring your own, your colleagues and your environment's safety.
Workplace safety is an important aspect to protect personnel against injury or serious accident.In case of animal cell culture safety takes a front seat due to nature of work i.e. handling of human cells and tissues, viruses with high potential to cause infections to humans and other adventitious micro organisms. This presentation presents various methods of safety to protect lab personnel from infectious biological agents.
Basics of BioSafety
This lesson will define and present information on
methods used to provide biosafety in facilities
where potentially infectious agents are used.
These include:
Containment
Biological safety cabinets
Personal protection equipment
The facility as barrier
Secondary barriers
Safety cabinets are intended to protect a laboratory worker from aerosols and airborne particles.
They will not protect the person from spillages and the consequences of mishandling and poor technique.
Aerosol particles of less than 5 µm in diameter and small droplets of 5–100 µm in diameter are not visible to the naked eye.
The laboratory worker is generally not aware that such particles are being generated and may be inhaled or may cross contaminate work surface materials.
BSCs, when properly used, have been shown to be highly effective in reducing laboratory-acquired infections and cross-contaminations of cultures due to aerosol exposures. BSCs also protect the environment.
Most BSCs use high efficiency particulate air (HEPA) filters in the exhaust and supply systems.
The exception is a Class I BSC, which does not have HEPA filtered supply air.
The document provides a detailed overview on the basic principles of operating a biotech or micro laboratory along with basic techniques with which to handle organisms, chemicals &equipment and ensuring your own, your colleagues and your environment's safety.
Barrier technique personal protective equipment [compatibility mode]drnahla
Infection Control Guidelines for appropriate use of personal protective equipment Barrier technique personal protective equipment
Dr. Nahla Abdel Kader.MD, PhD. Infection Control Consultant, MOH Infection Control Surveyor, CBAHI Infection Control Director,KKH.
Chlorinated paraffin (CP) is a complicated chemical that has a wide range of uses in a variety of industries. It is frequently used in rubber, paints, adhesives, caulks, sealants, and plastics as a secondary plasticizer and flame retardant. Another application is as a lubricant or coolant in the cutting or shaping of metal.
Elchemy is trusted chlorinated paraffin supplier, and exporter, offering high-quality and innovative chemical products to a wide range of industries. For more details, visit our website now!
https://www.elchemy.com/
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
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These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
2. Biological hazard Prevention and
Control
The first and the best strategy is to control and contain
the hazard at the source
Step in Biological Hazard Prevention & control
1. Substitute hazardous material with non-hazardous material
2. Design facility ( engineering control), use containment
equipment (primary containment), or design process to remove
the hazard or enclose the hazard to prevent exposure in
normal operations
3. Biological Hazard Prevention
and Control cont….
3. Where complete enclosure it not feasible,
establish barriers or local ventilation to reduce
exposure to the hazard in normal operations (PPE)
4. Work environment and the job itself should be
designed to eliminate hazards or reduce exposure
to hazards
4. Primary Barrier
Primary Containment Equipment
Primary containment contain the agent at the source
Biological Safety Cabinet, Fume Hood, Glove Box,
Animal Housing, Centrifuge
Personal Protective equipment (PPE)
PPE (Gloves, laboratory clothing, respirator etc..
PPE do not contain the hazard but protect the person
from exposure the hazard
5. Secondary containment / Barrier
Engineering control
Structure surrounding the primary barrier
Room, facilities…
Basic Laboratory
Containment Laboratory
Tertiary containment / Barrier
Area beyond the containment laboratory
Fence, corridor, building etc.
Significant and only high risk work
6. Hierarchy of Controls
TERTIARY CONTAINMENT
Area beyond the containment laboratory
Fence, corridor, building
SECONDARY CONTAINMENT
Laboratory, Facilities, containment laboratory
Engineering control
PRIMARY CONTAINMENT
BSC, Fume hood,
Centrifuge cups, room etc
PPE
1° Barrier
Gloves, Gown,
work practices etc
7. Personal Protective Equipment
To protect the wearer from
hazards, e.g. chemicals, dust, noise,
infectious agents
To prevent contamination of
wearer and the product
Minimize risk of exposure to
aerosols, splashes and
accidental inoculation
8. PPE- Laboratory Clothing
BSL-1 & 2
Front fastened lab coats
allowed
BSL-3
Wrap around gowns with
tight cuffs
BSL-4
Positive pressure suits
9. PPE-Footwear
Open-shoes and sandals not
acceptable for work with
infectious materials including
BSL-1
Use shoes with good non-slip
soles
Shoe covers add protection
Rubber boots with good soles
required if area is wet
10. PPE-Gloves
Vinyl gloves- Sensitive touch. Excellent
gloves for handing chemicals and
specimens during fixation and embedding
Nitrile gloves- made from a synthetic
latex. For users who are extremely allergic
to the protein found in natural latex.
Latex gloves (powdered or powdered
free)- Provides sensitive touch
Choose the right size for a good feel and grip
11. Removing Gloves Safety
3
2
You can get contaminated while removing gloves!
1. Grab the first glove
on the outer surface at
the wrist
2. Pull the glove back onto
itself so that when it is
completely removed it will
be inside out
3. Remove the other glove
by slipping your bare fingers
inside the glove, and pulling
it off so that it is also inside
out when completely
removed
Wash your hand after removing gloves!!!
Wash your hand after removing gloves!!!
13. Eyes and Face protection
• Goggles, safety glasses to protect the eyes
• Full face shield to protect facial skin protect
eyes from splashes
14. Respirators
Air Purifying and reduces aerosol
inhalation
Must have a proper fit lest for N-95 mask
Powered –PAPR
Air supplying
Air supplied from safe area
Supplied Air Respirator (SAR)
Air line and + pressure
Self Contained Breathing Apparatus
Consider for use in BSL-3 lab animal
facilities (rabbit, etc)
15. Primary Containment & Ventilation
Equipment
Personnel Product Environment
Chemical Fume
Hood
X
Laminar Flow
Clean Bench
X
Class I BSCs X X
Class II BSCs X X X
Class III BSCs X X X
Isolators X X X
21. Factor to consider when using
centrifuge
Risk group agent?
Route of transmission of
agent?
Volume to be centrifuged?
Where will the tubes be filled?
How will the rotor be loaded /
unloaded from the centrifuge?
Emergency response what
happens if a tube breaks in
centrifuge?
22. Procedures for Safety use of the
centrifuge
Always use safety cups and rotors
Load/unload rotor in BSC
Don’t overfill tubes
Clean and maintain gaskets and O
rings
Decontaminate centrifuge and
buckets it leakage occurs
If tube breaks in cycle wait minutes
for aerosols to clear before opening
23. Use the right centrifuge tubes, rotors, rotor cover,
o-ring etc..
Check for visible crack
Accident can happen
26. Managing fire safety
Develop fire safety policy
Define role and responsibilities
Develop and implement fire safety guidelines
Training and refresher course (theory and
practical)
Encourage external fire safety qualifications
and certifications
27. Managing fire safety
Evacuation procedures
Fire safety signage and notice
Regular maintenance of fire fighting
equipment
Regular audit and inspection
Good housekeeping to prevent
obstruction
29. General Principles
Faulty electrical appliance is one
of the leading causes of fire in
workplaces
Each electrical point has maximum
capacity for loading
Check the equipment before use
Understand the functions of the
equipment
Do not overload the electrical
circuits
biohazards, refer to organisms or organic matters produced by these organisms that are harmful to human health
So the best strategy to control and contain the hazard at the source
They have 4 strep to prevent & control
1. Substitute; some mechanism that can replacing to reproduce the hazards eg; work with big a mouth of volume of chemical bottle that we could aliquot in small bottle for in use
2. Design facility; avoided to used material produce or cause the hazard, because the air bone can effect worker eg; fume hood or BSC could remove air bone contamination
3. The PPE methods can protect you from biohazard.
Some PPE measure that can protect you for eg proper mask, latex gloves and eye protection
But the most informant the PPE to be effective it must be worn correctly and must be comfortable and fitted for each person.
4. Prevention and control systems should be designed to protect both workers’ health and the general environment.
1. It provides protection for personnel and the environment.
This equipment is important for hazardous material handling processes.
Including all equipment sub as BSC, FH,..they are have HEPA filters are used to in primary containments when microbial agents exists as aerosols.
2. PPE refers to protective clothing, helmets, goggles, or other equipment designed to protect the wearer's body from injury or infection.
Engineering control
Protection of the environment external to immediate laboratory through facility design and construction
structural aspects of the laboratory that make working environment safer against infection
Tertiary containment / Barrier
Area beyond the containment laboratory, need to think about risk assessment sub as fence, corridor or security
Selection the right of PPE as a barrier against skin, mucous membrane or respiratory exposure to infectious agents in the workplace
This for eg for Laboratory Clothing, the importan think the lab clothing should be long enough to cover knee or longer and long sleeve
Type of gloves also important if we could selected right type would comfortable to work
Vinyl gloves, Vinyl is less elastic than nitrile and latex, no powder
Nitrile is also a popular choice in the medical industry and for who are extremely allergic to the protein found in natural latex
latex gloves are more elastic
Respirators. protect the wearer from inhaling particulate matter, including airborne microorganisms, fumes, vapours
Must have a proper fit lest for N-95 mask
Air supply it the supply the clean air directly to the worker suit
Fire hazards it the potential to cause harm in terms of injury, ill health, or damage and include all types of live flames, causes of sparks, hot objects, and chemicals that are potential for ignition
It refers to planning and infrastructure in an organization that's designed to reduce fires and their effects.
Electrical hazard are another common laboratory hazard, which can occur when incorrect or unsafe cords and plugs are used. Any electrical appliances used in the laboratory should be fit for purpose, up-to-date and correspond to connected devices before they are implemented. Any electrical apparatus, from adaptors to cables, not safety-tested could compromise the safety of the lab and research team.
Who are at risk? 1) agricultural workers 2) business establishments workers 3) construction workers 4) transport workers
Low flash point, fuel source, may ignite spontaneously from spark, flame or high temperature
Cylinders of compressed gas are under intense pressure, sudden release
Strong acids and bases can cause visible destruction of living cells upon contract, or property damage
Many substances will react violently if allowed contract with air, water, other chemicals or with light or heat
Many back injuries are completely avoidable by using the proper techniques for lifting.
reminds people to never pick up a heavy item on their own because it's far better to get assistance and lift as a team.
It is caused by prolonged exposure to noise at work which results in the damage of the nerve cells of the inner ear. Who are at risk? Farmers, civil engineers, construction workers, factory workers, mining engineers, musicians and music teachers, airline workers, police, soldiers, pilots