This document discusses bioremediation, which uses living organisms like microbes and plants to break down and consume environmental pollutants. It can be done through microbial remediation using intrinsic or engineered microbes, or phyto-remediation using plants. Methods include in-situ techniques like bioventing and biosparging as well as ex-situ ones like biopiles and landfarming. While bioremediation is natural and can control pollution, it is limited to biodegradable wastes and specific processes, and ex-situ methods may disperse pollutants.
This document discusses bioremediation, which uses microorganisms to remove pollution from soil, water, and air. There are two types of bioremediation - in situ, which treats pollution at the site, and ex situ, which treats pollution off site. In situ bioremediation can be intrinsic, using native microbes, or engineered, by adding nutrients or microbes. Ex situ involves removing contaminated material and treating it through methods like slurry phase bioremediation, which mixes soil and water, or solid phase bioremediation using land farming or piles. Bioremediation is effective but performance is difficult to evaluate and volatile organic compounds remain challenging to degrade.
• Bioremediation – process of cleaning up environmental sites contaminated with chemical pollutants by using living organisms to degrade hazardous materials into less toxic substances
This document discusses intrinsic in situ bioremediation. It explains that intrinsic bioremediation uses microorganisms already present in the environment to degrade contaminants, requiring no human intervention and being the cheapest form of bioremediation. Intrinsic bioremediation is tested at the lab and field levels before use to assess the microorganisms' ability to metabolize contaminants. Factors like moisture, pH, temperature, nutrients, electron acceptors, and toxin concentration affect the rate of intrinsic bioremediation. In situ bioremediation cleans up contaminated sites directly where pollution occurred, with options like biostimulation or bioaugmentation. It has advantages of being cost-effective with minimal exposure but
A Recent Technique for Contaminated Soils: BioremediationThe Funtasty
This document discusses bioremediation techniques for cleaning up contaminated soils. It defines bioremediation as using microorganisms, fungi or plants to restore a natural environment impacted by pollution. Methods include stimulating indigenous bacteria to break down oil spills or other contaminants, and augmenting soils with bacteria to degrade pollutants. Bioremediation can occur in situ or ex situ and examples provided are bioventing and phytoremediation using plants to extract heavy metals. The advantages highlighted are lower costs than traditional methods while preserving the natural environment.
In Situ Bioremediation;Types, Advantages and limitations Zohaib HUSSAIN
In situ bioremediation uses microorganisms to treat hazardous waste in place, without removing the contaminated material. It can be applied in both the unsaturated zone (e.g. bioventing) and saturated zones (groundwater). Intrinsic bioremediation relies on naturally occurring microbes, while engineered approaches accelerate degradation by supplying oxygen, nutrients, or other stimulants. Successful in situ bioremediation is evidenced by measuring increased microbial activity, growth of degrading populations, and production of degradation byproducts at the site.
This ppt contains all types of Microbial Bioremediation methods . Everyone can understand clearly . Explaining with neat pictures and animation . Useful for presentation about Microbes in bioremediation . At last it contains a small animated video which helps to get clear view .
This document discusses bioremediation, which uses living organisms like microbes and plants to break down and consume environmental pollutants. It can be done through microbial remediation using intrinsic or engineered microbes, or phyto-remediation using plants. Methods include in-situ techniques like bioventing and biosparging as well as ex-situ ones like biopiles and landfarming. While bioremediation is natural and can control pollution, it is limited to biodegradable wastes and specific processes, and ex-situ methods may disperse pollutants.
This document discusses bioremediation, which uses microorganisms to remove pollution from soil, water, and air. There are two types of bioremediation - in situ, which treats pollution at the site, and ex situ, which treats pollution off site. In situ bioremediation can be intrinsic, using native microbes, or engineered, by adding nutrients or microbes. Ex situ involves removing contaminated material and treating it through methods like slurry phase bioremediation, which mixes soil and water, or solid phase bioremediation using land farming or piles. Bioremediation is effective but performance is difficult to evaluate and volatile organic compounds remain challenging to degrade.
• Bioremediation – process of cleaning up environmental sites contaminated with chemical pollutants by using living organisms to degrade hazardous materials into less toxic substances
This document discusses intrinsic in situ bioremediation. It explains that intrinsic bioremediation uses microorganisms already present in the environment to degrade contaminants, requiring no human intervention and being the cheapest form of bioremediation. Intrinsic bioremediation is tested at the lab and field levels before use to assess the microorganisms' ability to metabolize contaminants. Factors like moisture, pH, temperature, nutrients, electron acceptors, and toxin concentration affect the rate of intrinsic bioremediation. In situ bioremediation cleans up contaminated sites directly where pollution occurred, with options like biostimulation or bioaugmentation. It has advantages of being cost-effective with minimal exposure but
A Recent Technique for Contaminated Soils: BioremediationThe Funtasty
This document discusses bioremediation techniques for cleaning up contaminated soils. It defines bioremediation as using microorganisms, fungi or plants to restore a natural environment impacted by pollution. Methods include stimulating indigenous bacteria to break down oil spills or other contaminants, and augmenting soils with bacteria to degrade pollutants. Bioremediation can occur in situ or ex situ and examples provided are bioventing and phytoremediation using plants to extract heavy metals. The advantages highlighted are lower costs than traditional methods while preserving the natural environment.
In Situ Bioremediation;Types, Advantages and limitations Zohaib HUSSAIN
In situ bioremediation uses microorganisms to treat hazardous waste in place, without removing the contaminated material. It can be applied in both the unsaturated zone (e.g. bioventing) and saturated zones (groundwater). Intrinsic bioremediation relies on naturally occurring microbes, while engineered approaches accelerate degradation by supplying oxygen, nutrients, or other stimulants. Successful in situ bioremediation is evidenced by measuring increased microbial activity, growth of degrading populations, and production of degradation byproducts at the site.
This ppt contains all types of Microbial Bioremediation methods . Everyone can understand clearly . Explaining with neat pictures and animation . Useful for presentation about Microbes in bioremediation . At last it contains a small animated video which helps to get clear view .
Bioremediation - prospects for the future application of innovative appliedIvan Vera Montenegro
1) Bioremediation uses biological processes to eliminate, attenuate, or transform polluting substances. Traditional techniques like biopiling and landfarming rely on microbial degradation of contaminants in soil. Phytoremediation uses plants and their rhizospheres to uptake or degrade contaminants.
2) Phytobial remediation combines phytoremediation and bioremediation by using microbes like Trichoderma harzianum colonized in plant roots to efficiently degrade toxicants while providing an energy source from plant root exudates.
3) Initial experiments found T. harzianum could detoxify cyanides and metallocyanides in soil, allowing plant
This document discusses bioremediation, which is a process that uses microorganisms or plant enzymes to treat contaminated media like water or soil. There are two main types of bioremediation: in-situ, which remediates contamination at the site, and ex-situ, which treats contaminated materials after removing them from the site. Selection of bioremediation technologies depends on factors like the type of microorganisms, pollutant concentration, environment, and nutrient availability. Common in-situ technologies include biostimulation, bioaugmentation, bioventing, and biosparging.
Bioremediation uses microorganisms like bacteria and fungi to degrade contaminants in soil and water. It works by stimulating natural microbial activity to break down harmful pollutants into harmless substances. Various technologies can be used including treating excavated soil in biopiles or bioreactors, injecting nutrients and oxygen into contaminated groundwater and soil, and planting vegetation that helps remove toxins from the environment. The microbes metabolize the pollutants for food and energy through aerobic or anaerobic processes, transforming contaminants into less toxic or non-toxic forms.
This document discusses bioremediation, which uses microorganisms to degrade environmental pollutants. It defines bioremediation and describes how it works to break down hazardous substances. There are three main types of bioremediation: biostimulation adds nutrients to stimulate microbial growth; bioaugmentation adds microbes to degrade specific contaminants; and intrinsic bioremediation relies on natural attenuation. Methods of bioremediation include in-situ and ex-situ approaches. In-situ techniques like bioventing treat pollution on-site, while ex-situ methods involve removing material to above-ground bioreactors for treatment. Bioremediation has applications in controlling water, soil and air
This document provides an overview of bioremediation presented by Md. Shoyeb. It defines bioremediation as using organisms or their enzymes to return polluted environments to their original condition. The mechanisms, principles, strategies (in situ and ex situ), types (bioventing, biosparging, bioaugmentation), advantages and disadvantages are summarized. Key contaminants amenable to bioremediation are identified along with persistent pollutants that are difficult to degrade.
This document discusses bioremediation and the enzymes used in the process. It begins with background information on bioremediation and enzymes. Major enzymes that aid in bioremediation are then outlined, including peroxidases, oxygenases, and dioxygenases. An example is given of lignin peroxidase and its effectiveness in bioremediating pollutants. The advantages of bioremediation include it being relatively inexpensive and allowing toxic waste to naturally break down. Limitations include difficulty controlling bacteria and potential to spread illness. In conclusion, bioremediation offers a safer, more cost-effective cleaning method for contaminated sites.
Bioremediation is the process in which the micro-organisms are used to degrade the pollutants from the environment. Plants and micro-organisms are used to clean up the environment. Bioremediation is carried out by microbes and their metabolisms are used to remove the contaminants. Microbes have the ability to resolve the issue of contaminated ecosystem1. To improve or better living style the degradation of contaminated areas is very important. Importance of the biodegradation is increasing due to the expensiveness of the chemicals. So bioremediation is the best choice. The effluents should be degraded from the environment because they are very dangerous and have a bad impact on human beings. These pollutants sink into the water and cause pollution. These pollutants are treated with the help of microbes in bioremediation process. It is the best method because it is cost effective and eco-friendly. Different techniques of bioremediation are used to convert toxic substances into less toxic substances.
Bioremediation is the use of microorganisms (e.g., bacteria, fungi), plants (termed phytoremediation), or biological enzymes to achieve treatment of hazardous waste. Treatment can target a variety of media (wastewater, groundwater, soil/sludge, gas) with several possible objectives (e.g., mineralization of organic compounds, immobilzation of contaminants). In situ bioremediation (ISB) is the application of bioremediation in the subsurface – as compared to ex situ bioremediation, which applies to media readily accessible aboveground (e.g., in treatment cells/soil piles or bioreactors). In situ bioremediation may be applied in the unsaturated/vadoze zone (e.g., bioventing) or in saturated soils and groundwater (Sharma S. 2012).
The document discusses bioremediation techniques for treating fish processing waste. It provides background on the large quantities of solid waste and wastewater generated by fish processing plants. Both aerobic and anaerobic bioremediation techniques can be used, including intrinsic and accelerated bioremediation which use indigenous or added microorganisms. Specific in situ techniques mentioned are bioventing, biostimulation, and bioaugmentation. Essential factors for effective microbial bioremediation include suitable microbial populations, oxygen, water, nutrients, temperature, and pH. Bioremediation is seen as a cost effective and environmentally friendly way to treat fish processing waste and other pollutants.
Phytoremediation is defined as the use of higher plants for the cost-effective, environmentally friendly rehabilitation of soil and groundwater contaminated by toxic metals and organic compounds.
Dissertation ppt biostimulation- a potential practice for wastewater treaat...Sumer Pankaj
Phycoremediation is a green technology that supports the direct use of living green microalgae for in situ, or in place removal, degradation, of contaminants in soils, sludge, sediments, surface water and ground waters by the mechanisms of bio-transformation, bio-accumulation, bio-concentration, bio-sparging.
It can be said by the current study that microalgae has a great potential for the treatment of industrial and municipal wastewaters as compared to the chemical treatments available commercially. Biological systems are much more efficient in cleaning the excess nutrients from the waste water followed by generation of valuable biomass which can be applied in the food, fertilizer, energy production as use of inorganic chemicals like lime and ferrous sulphate generates huge amount of sludge in textile industries, but on the other hand static anaerobic treatment using acclimatized MLSS gives better colour reduction with zero sludge generation. Microalgal cells can be used in free form to treat waste waters containing high C.O.D., high ammonical nitrogen and high TDS. It not only provides a better reduction of chemicals from wastewaters but it also helps to reduce the operational cost of ETP. Microalgaes not only helps to remediate industrial waste waters but also to treat sweage water and to restore natural water bodies like lakes and ponds. As they are active in remediating the chemicals but also it shows an antagonistic effect against some pathogenic germs like total coliforms and fecal coliforms.
These microalgal cells can also be combined with bacterial biomass of activated sludge process to develop an Algal-Bacterial consortium (ALBA) for better enhancement in the reduction of chemicals from the wastewaters as this symbiotic relation of algae and bacteria provides high satiability of the microalgae along with MLSS and faceable in terms of price and economy for instance the bacterial biomass provides carbon dioxide to algal cells for photosynthesis and in return the bacteria acquires oxygen from algae. The harvested biomass from the ETP’s can be used as bio-fertilizers as it consists of appropriate ratio of vital macro and micro nutrients like N,P,K etc. which enhance the growth of plantlets. It can also be used as aqua feeds for shrimps, fishes and molluscs. Furthermore these microlgal cells are non-toxic in the environment as it becomes a part of food chain and do not cause eutrophication. Therefore, micro-algal based treatment is most suitable for the treating the waste waters and restoring the natural water bodies as compared to other chemical treatments.
•Introduction of bioremediation: Bioremediation refers to the process of using microorganisms to remove the environmental pollutants i.e. toxic wastes found in soil, water, air etc.
•In situ bioremediation:
It involves a direct approach for the microbial
degradation of xenobiotics at the sites of pollution
(soil, ground water).
•Types of in situ bioremediation:
Natural attenuation.
Engineered in situ bioremediation.
- Bioventing, biosparging, bioslurping,
phytoremediation.
•Ex situ bioremediation:
Waste or toxic pollutants can be collected from the polluted sites and bioremediation can be carried out at a designated place or site.
• Types of ex situ bioremediation
Land farming, windrow, biopiles, bioreactors.
•Microorganisms use in bioremediation:
A number of naturally occurring marine microbes
such as Pseudomonas sp. is capable of degrading oil and other hydrocarbons.
•Factors affecting bioremediation:
Nutrient availability, moisture content, pH, temperature, contaminant availability.
•References:
Satyanarayana U. Biotechnology. BOOKS AND ALLIED (P) Ltd.
Sharma P.D. Environmental Microbiology. RASTOGI PUBLICATIONS.
Gupta P.K. Biotechnology and Genomics. RASTOGI PUBLICATIONS.
Dubey R.C. A Textbook of Biotechnology. S Chand And Company Ltd.
Dubey R.C. A Textbook of Microbiology. S Chand And Company Ltd.
Willey/Sherwood/Woolverton. Prescott’s Microbiology. McGRAW-HILL INTERNATIONAL EDITION.
www.sciencedirect.com/bioremediation.
The document discusses bioremediation, which uses microorganisms to degrade environmental pollutants. It describes different types of bioremediation including in situ and ex situ methods. In situ bioremediation occurs on-site and can be intrinsic or engineered, while ex situ involves removing contaminated material for treatment using methods like land farming, composting, or biopiles. The document also outlines factors influencing bioremediation and lists some advantages and limitations.
This document provides information about bioremediation. It begins with an introduction defining bioremediation as using microorganisms to degrade hazardous chemicals into less toxic forms. It then discusses the types of microorganisms involved, including Pseudomonas genus and Xenobiotics-degrading microorganisms. Several examples of pollutants and degrading microorganisms are given. The mechanisms of bioremediation include aerobic and anaerobic transformations such as respiration, fermentation, and methane fermentation. Factors affecting bioremediation like moisture, nutrients, oxygen levels, pH, temperature, and pollutant characteristics are outlined. Methods of bioremediation include in-situ and ex-situ techniques
Bioremediation uses microorganisms to break down hazardous substances into less toxic forms. There are two main techniques: in-situ treats contaminated soil and groundwater in place without excavation, while ex-situ excavates soil prior to treatment using methods like biopiles, bioreactors, land farming, and windrows that optimize conditions for microorganisms. The document discusses various enhanced bioremediation methods for both in-situ and ex-situ treatment.
The document discusses bioremediation, which uses microorganisms to break down environmental pollutants and clean contaminated sites. It describes different types of bioremediation including microbial remediation, which uses bacteria and fungi, and phytoremediation, which uses plants. The goals, methods, applications, advantages and limitations of bioremediation are summarized. Key bioremediation techniques mentioned are bioventing, land-farming, bioaugmentation, and biopiles.
This document discusses bioremediation, which uses microorganisms like bacteria and fungi to degrade environmental pollutants. It defines bioremediation and describes how it works by stimulating existing microbes or adding specialized microbes. The key factors for effective bioremediation like nutrients, water, oxygen and temperature are outlined. In-situ and ex-situ bioremediation methods are compared, and applications to treat soil, groundwater, marine spills and air are reviewed. Advantages like low cost are balanced with longer timescales. Related technologies like phytoremediation and bioventing are also mentioned.
This document discusses the roles of microbes in bioremediation. It defines bioremediation as using microorganisms such as bacteria and fungi to degrade contaminants in soils, groundwater, and sediments. The key factors that affect microbial bioremediation are discussed, including the microbial population, oxygen, water, nutrients, temperature, and pH. Different types of bioremediation techniques are described, as well as examples of bioremediation applications in soils, groundwater, marine oil spills, and air pollution control.
Bioremediation - prospects for the future application of innovative appliedIvan Vera Montenegro
1) Bioremediation uses biological processes to eliminate, attenuate, or transform polluting substances. Traditional techniques like biopiling and landfarming rely on microbial degradation of contaminants in soil. Phytoremediation uses plants and their rhizospheres to uptake or degrade contaminants.
2) Phytobial remediation combines phytoremediation and bioremediation by using microbes like Trichoderma harzianum colonized in plant roots to efficiently degrade toxicants while providing an energy source from plant root exudates.
3) Initial experiments found T. harzianum could detoxify cyanides and metallocyanides in soil, allowing plant
This document discusses bioremediation, which is a process that uses microorganisms or plant enzymes to treat contaminated media like water or soil. There are two main types of bioremediation: in-situ, which remediates contamination at the site, and ex-situ, which treats contaminated materials after removing them from the site. Selection of bioremediation technologies depends on factors like the type of microorganisms, pollutant concentration, environment, and nutrient availability. Common in-situ technologies include biostimulation, bioaugmentation, bioventing, and biosparging.
Bioremediation uses microorganisms like bacteria and fungi to degrade contaminants in soil and water. It works by stimulating natural microbial activity to break down harmful pollutants into harmless substances. Various technologies can be used including treating excavated soil in biopiles or bioreactors, injecting nutrients and oxygen into contaminated groundwater and soil, and planting vegetation that helps remove toxins from the environment. The microbes metabolize the pollutants for food and energy through aerobic or anaerobic processes, transforming contaminants into less toxic or non-toxic forms.
This document discusses bioremediation, which uses microorganisms to degrade environmental pollutants. It defines bioremediation and describes how it works to break down hazardous substances. There are three main types of bioremediation: biostimulation adds nutrients to stimulate microbial growth; bioaugmentation adds microbes to degrade specific contaminants; and intrinsic bioremediation relies on natural attenuation. Methods of bioremediation include in-situ and ex-situ approaches. In-situ techniques like bioventing treat pollution on-site, while ex-situ methods involve removing material to above-ground bioreactors for treatment. Bioremediation has applications in controlling water, soil and air
This document provides an overview of bioremediation presented by Md. Shoyeb. It defines bioremediation as using organisms or their enzymes to return polluted environments to their original condition. The mechanisms, principles, strategies (in situ and ex situ), types (bioventing, biosparging, bioaugmentation), advantages and disadvantages are summarized. Key contaminants amenable to bioremediation are identified along with persistent pollutants that are difficult to degrade.
This document discusses bioremediation and the enzymes used in the process. It begins with background information on bioremediation and enzymes. Major enzymes that aid in bioremediation are then outlined, including peroxidases, oxygenases, and dioxygenases. An example is given of lignin peroxidase and its effectiveness in bioremediating pollutants. The advantages of bioremediation include it being relatively inexpensive and allowing toxic waste to naturally break down. Limitations include difficulty controlling bacteria and potential to spread illness. In conclusion, bioremediation offers a safer, more cost-effective cleaning method for contaminated sites.
Bioremediation is the process in which the micro-organisms are used to degrade the pollutants from the environment. Plants and micro-organisms are used to clean up the environment. Bioremediation is carried out by microbes and their metabolisms are used to remove the contaminants. Microbes have the ability to resolve the issue of contaminated ecosystem1. To improve or better living style the degradation of contaminated areas is very important. Importance of the biodegradation is increasing due to the expensiveness of the chemicals. So bioremediation is the best choice. The effluents should be degraded from the environment because they are very dangerous and have a bad impact on human beings. These pollutants sink into the water and cause pollution. These pollutants are treated with the help of microbes in bioremediation process. It is the best method because it is cost effective and eco-friendly. Different techniques of bioremediation are used to convert toxic substances into less toxic substances.
Bioremediation is the use of microorganisms (e.g., bacteria, fungi), plants (termed phytoremediation), or biological enzymes to achieve treatment of hazardous waste. Treatment can target a variety of media (wastewater, groundwater, soil/sludge, gas) with several possible objectives (e.g., mineralization of organic compounds, immobilzation of contaminants). In situ bioremediation (ISB) is the application of bioremediation in the subsurface – as compared to ex situ bioremediation, which applies to media readily accessible aboveground (e.g., in treatment cells/soil piles or bioreactors). In situ bioremediation may be applied in the unsaturated/vadoze zone (e.g., bioventing) or in saturated soils and groundwater (Sharma S. 2012).
The document discusses bioremediation techniques for treating fish processing waste. It provides background on the large quantities of solid waste and wastewater generated by fish processing plants. Both aerobic and anaerobic bioremediation techniques can be used, including intrinsic and accelerated bioremediation which use indigenous or added microorganisms. Specific in situ techniques mentioned are bioventing, biostimulation, and bioaugmentation. Essential factors for effective microbial bioremediation include suitable microbial populations, oxygen, water, nutrients, temperature, and pH. Bioremediation is seen as a cost effective and environmentally friendly way to treat fish processing waste and other pollutants.
Phytoremediation is defined as the use of higher plants for the cost-effective, environmentally friendly rehabilitation of soil and groundwater contaminated by toxic metals and organic compounds.
Dissertation ppt biostimulation- a potential practice for wastewater treaat...Sumer Pankaj
Phycoremediation is a green technology that supports the direct use of living green microalgae for in situ, or in place removal, degradation, of contaminants in soils, sludge, sediments, surface water and ground waters by the mechanisms of bio-transformation, bio-accumulation, bio-concentration, bio-sparging.
It can be said by the current study that microalgae has a great potential for the treatment of industrial and municipal wastewaters as compared to the chemical treatments available commercially. Biological systems are much more efficient in cleaning the excess nutrients from the waste water followed by generation of valuable biomass which can be applied in the food, fertilizer, energy production as use of inorganic chemicals like lime and ferrous sulphate generates huge amount of sludge in textile industries, but on the other hand static anaerobic treatment using acclimatized MLSS gives better colour reduction with zero sludge generation. Microalgal cells can be used in free form to treat waste waters containing high C.O.D., high ammonical nitrogen and high TDS. It not only provides a better reduction of chemicals from wastewaters but it also helps to reduce the operational cost of ETP. Microalgaes not only helps to remediate industrial waste waters but also to treat sweage water and to restore natural water bodies like lakes and ponds. As they are active in remediating the chemicals but also it shows an antagonistic effect against some pathogenic germs like total coliforms and fecal coliforms.
These microalgal cells can also be combined with bacterial biomass of activated sludge process to develop an Algal-Bacterial consortium (ALBA) for better enhancement in the reduction of chemicals from the wastewaters as this symbiotic relation of algae and bacteria provides high satiability of the microalgae along with MLSS and faceable in terms of price and economy for instance the bacterial biomass provides carbon dioxide to algal cells for photosynthesis and in return the bacteria acquires oxygen from algae. The harvested biomass from the ETP’s can be used as bio-fertilizers as it consists of appropriate ratio of vital macro and micro nutrients like N,P,K etc. which enhance the growth of plantlets. It can also be used as aqua feeds for shrimps, fishes and molluscs. Furthermore these microlgal cells are non-toxic in the environment as it becomes a part of food chain and do not cause eutrophication. Therefore, micro-algal based treatment is most suitable for the treating the waste waters and restoring the natural water bodies as compared to other chemical treatments.
•Introduction of bioremediation: Bioremediation refers to the process of using microorganisms to remove the environmental pollutants i.e. toxic wastes found in soil, water, air etc.
•In situ bioremediation:
It involves a direct approach for the microbial
degradation of xenobiotics at the sites of pollution
(soil, ground water).
•Types of in situ bioremediation:
Natural attenuation.
Engineered in situ bioremediation.
- Bioventing, biosparging, bioslurping,
phytoremediation.
•Ex situ bioremediation:
Waste or toxic pollutants can be collected from the polluted sites and bioremediation can be carried out at a designated place or site.
• Types of ex situ bioremediation
Land farming, windrow, biopiles, bioreactors.
•Microorganisms use in bioremediation:
A number of naturally occurring marine microbes
such as Pseudomonas sp. is capable of degrading oil and other hydrocarbons.
•Factors affecting bioremediation:
Nutrient availability, moisture content, pH, temperature, contaminant availability.
•References:
Satyanarayana U. Biotechnology. BOOKS AND ALLIED (P) Ltd.
Sharma P.D. Environmental Microbiology. RASTOGI PUBLICATIONS.
Gupta P.K. Biotechnology and Genomics. RASTOGI PUBLICATIONS.
Dubey R.C. A Textbook of Biotechnology. S Chand And Company Ltd.
Dubey R.C. A Textbook of Microbiology. S Chand And Company Ltd.
Willey/Sherwood/Woolverton. Prescott’s Microbiology. McGRAW-HILL INTERNATIONAL EDITION.
www.sciencedirect.com/bioremediation.
The document discusses bioremediation, which uses microorganisms to degrade environmental pollutants. It describes different types of bioremediation including in situ and ex situ methods. In situ bioremediation occurs on-site and can be intrinsic or engineered, while ex situ involves removing contaminated material for treatment using methods like land farming, composting, or biopiles. The document also outlines factors influencing bioremediation and lists some advantages and limitations.
This document provides information about bioremediation. It begins with an introduction defining bioremediation as using microorganisms to degrade hazardous chemicals into less toxic forms. It then discusses the types of microorganisms involved, including Pseudomonas genus and Xenobiotics-degrading microorganisms. Several examples of pollutants and degrading microorganisms are given. The mechanisms of bioremediation include aerobic and anaerobic transformations such as respiration, fermentation, and methane fermentation. Factors affecting bioremediation like moisture, nutrients, oxygen levels, pH, temperature, and pollutant characteristics are outlined. Methods of bioremediation include in-situ and ex-situ techniques
Bioremediation uses microorganisms to break down hazardous substances into less toxic forms. There are two main techniques: in-situ treats contaminated soil and groundwater in place without excavation, while ex-situ excavates soil prior to treatment using methods like biopiles, bioreactors, land farming, and windrows that optimize conditions for microorganisms. The document discusses various enhanced bioremediation methods for both in-situ and ex-situ treatment.
The document discusses bioremediation, which uses microorganisms to break down environmental pollutants and clean contaminated sites. It describes different types of bioremediation including microbial remediation, which uses bacteria and fungi, and phytoremediation, which uses plants. The goals, methods, applications, advantages and limitations of bioremediation are summarized. Key bioremediation techniques mentioned are bioventing, land-farming, bioaugmentation, and biopiles.
This document discusses bioremediation, which uses microorganisms like bacteria and fungi to degrade environmental pollutants. It defines bioremediation and describes how it works by stimulating existing microbes or adding specialized microbes. The key factors for effective bioremediation like nutrients, water, oxygen and temperature are outlined. In-situ and ex-situ bioremediation methods are compared, and applications to treat soil, groundwater, marine spills and air are reviewed. Advantages like low cost are balanced with longer timescales. Related technologies like phytoremediation and bioventing are also mentioned.
This document discusses the roles of microbes in bioremediation. It defines bioremediation as using microorganisms such as bacteria and fungi to degrade contaminants in soils, groundwater, and sediments. The key factors that affect microbial bioremediation are discussed, including the microbial population, oxygen, water, nutrients, temperature, and pH. Different types of bioremediation techniques are described, as well as examples of bioremediation applications in soils, groundwater, marine oil spills, and air pollution control.
Bioremediation
Bioremediation refers to the use of either naturally occurring or
deliberately introduced microorganisms to consume and break down
environmental pollutants, in order to clean a polluted site.
The process of bioremediation enhances the rate of the natural
microbial degradation of contaminants by supplementing the
indigenous microorganisms (bacteria or fungi) with nutrients, carbon
sources, or electron donors (biostimulation, biorestoration) or by
adding an enriched culture of microorganisms that have specific
characteristics that allow them to degrade the desired contaminant at
a quicker rate (bioaugmentation).
It is a cleaning process that degrades dangerous contaminants using
naturally existing microbes. These bacteria may consume and
degrade organic chemicals as a source of food and energy, degrade
organic substances that are dangerous to living creatures, including
humans, and degrade the organic pollutants into inert products.
Because the bacteria already exist in nature, they offer no pollution
concern
Bioremediation is the use of
microorganisms or microbial processes
to detoxify and degrade environmental
contaminants.
Microorganisms have been used for the
routine treatment and transformation
of waste products for several decades
Bioremediation strategies rely on
having the correct microorganisms in
the right location at the right time in the
right environment for degradation to
occur. The appropriate microorganisms
are bacteria and fungi that have the
physiological and metabolic
competence to breakdown pollutants
Objective of Bioremediation
The objective of bioremediation is to decrease pollutant levels to
undetectable, nontoxic, or acceptable levels, i.e., within regulatory
limits, or, ideally, to totally mineralize organopollutants to carbon
dioxide
BIOREMEDIATION AND THEIR IMPORTANCE IN ENVIRONMENT
PROTECTION
Bioremediation is defined as ‘the process of using microorganisms to remove
the environmental pollutants where microbes serve as scavengers’.
• The removal of organic wastes by microbes leads to environmental clean-up.
The other names/terms used for bioremediation are biotreatment,
bioreclamation, and biorestoration.
• The term “Xenobiotics” (xenos means foreign) refers to the unnatural, foreign
and synthetic chemicals, such as pesticides, herbicides, refrigerants, solvents
and other organic compounds.
• The microbial degradation of xenobiotics also helps in reducing the
environmental pollution. Pseudomonas which is a soil microorganism
effectively degrades xenobiotics.
• Different strains of Pseudomonas that are capable of detoxifying more than
100 organic compounds (e.g. phenols, biphenyls, organophosphates,
naphthalene, etc.) have been identified.
• Some other microbial strains are also known to have the capacity to degrade
xenobiotics such as Mycobacterium, Alcaligenes, Norcardia, etc.
Factors affecting biodegradation
The factors that affect the
biodegradation are:
• the chemical nature of
xenobiotics,
• the conc
This document discusses bioremediation of contaminated soils. It defines bioremediation as using microorganisms or their enzymes to degrade soil contaminants like heavy metals, pesticides, and hydrocarbons. Two main types are discussed - intrinsic bioremediation which uses native microbes, and engineered bioremediation which introduces microbes. Engineered techniques include biostimulation to improve conditions for native microbes, bioventing to add oxygen, and bioaugmentation to add specific degrading microbes. The document provides details on various bioremediation processes and criteria for their effective use.
This document discusses various types of bioremediation techniques used to clean up contaminated soil and groundwater. It defines bioremediation as using living microorganisms to degrade environmental pollutants or prevent pollution. The two main types of bioremediation are in situ, which treats contaminants in place, and ex situ, which involves removing contaminated material to be treated elsewhere. Specific techniques discussed include bioaugmentation, bioslurping, biosparging, natural attenuation, bioventing, and biostimulation. The advantages and limitations of bioremediation are also summarized.
This document discusses bioremediation, which uses microorganisms to degrade environmental contaminants into less toxic forms. It describes various methods of bioremediation including bioaugmentation, biostimulation, and intrinsic bioremediation. Bioaugmentation involves adding microbes that can degrade specific contaminants, biostimulation provides nutrients to promote existing microbial growth, and intrinsic bioremediation relies on natural microbial activity. The document also outlines the types of microbes used in bioremediation such as bacteria, fungi, algae, and plants. It concludes that bioremediation is an effective technique for reducing environmental toxicity and discusses using algae to treat wastewater and metal-hyperaccumulating plants for ph
Micro-organisms are well known for their ability to break down a huge range of organic compounds and absorb inorganic substances. Currently, microbes are used to clean up pollution treatment in processes known as ‘bioremediation’.
This document provides an overview of bioremediation, which uses microorganisms like bacteria and fungi to degrade contaminants in soil, water, and other environments. It discusses how bioremediation works, essential factors for effective microbial bioremediation, different bioremediation methods, microbes involved, advantages and disadvantages, applications, and related technologies. The key points are that bioremediation relies on microbes breaking down pollutants into less toxic forms, various methods can be used like biostimulation or bioaugmentation, and it has benefits of being low-cost and minimizing site disruption while drawbacks include being time-consuming and not working for all compounds.
The document discusses bioremediation, which uses microorganisms to break down environmental pollutants. It can be used to treat sites contaminated with substances like oil, solvents, and pesticides. There are two main types - microbial remediation which uses bacteria and fungi, and phytoremediation which uses various plant species. The goal is to reduce pollutant levels to safe levels set by regulatory agencies through stimulating microbial growth and degradation of contaminants.
Bioremediation is a process that uses microorganisms to degrade contaminants in various media like water, soil, and subsurface materials. There are three main types of bioremediation: biostimulation adds nutrients to stimulate microbial growth; bioaugmentation adds specialized microbes to sites where indigenous microbes cannot fully degrade contaminants; and intrinsic bioremediation relies on natural microbial attenuation in soils and waters. Bioremediation depends on microbial metabolism, where microbes use contaminants for energy and building cell materials through catabolic and anabolic processes.
Bioremediation uses microorganisms like bacteria and fungi to degrade environmental pollutants into less toxic forms. It can occur in situ, with the contaminants treated on site, or ex situ by removing the contaminated soil or water for treatment. The mechanisms involve microbes breaking down pollutants for food and energy. Key types are bacterioremediation using bacteria, phytoremediation using plants, and mycoremediation using fungi. Bioremediation can transform or mineralize organic wastes into innocuous substances like carbon dioxide and water.
The document discusses various methods of bioremediation and biodegradation to remediate contaminated soil and groundwater. It defines bioremediation as using biological organisms such as bacteria and fungi to solve environmental problems through technological innovation. Biodegradation is the natural breakdown of materials by microorganisms. The document then describes various in situ and ex situ bioremediation techniques in detail, including bioventing, biosparging, bioslurping, phytoremediation, land farming, biopiles, and windrows. The key factors in selecting a bioremediation method are the contaminants present, their accessibility to microbes, and any environmental conditions that could inhibit microbial activity.
Bioremediation uses microorganisms to break down contaminants in soil and water. There are three main types: biostimulation adds nutrients to encourage microbial growth; bioaugmentation adds microbes that degrade specific contaminants; and intrinsic bioremediation relies on naturally occurring microbes. Microbes metabolize contaminants through anabolism and catabolism, using contaminants for energy and building cell structures. Factors like microbial populations, contaminant availability, temperature, and nutrients influence bioremediation effectiveness.
This document provides an overview of bioremediation and phytoremediation. It defines bioremediation as using biological organisms like microbes and plants to treat contaminated soil and water. The document discusses the history of bioremediation and categorizes different bioremediation techniques. It also outlines the pros and cons of various in-situ and ex-situ bioremediation methods like bioventing, bioaugmentation, biostimulation, biosparging, land farming and composting. Finally, it introduces the concept of phytoremediation and notes that it involves using plants to remediate environmental contaminants.
Biodegradation is very fruitful and attractive option to remediating, cleaning, managing and recovering technique for solving polluted environment through microbial activity. The speed of undesirable waste substances degradation is determined in competition within biological agents like fungi, bacterial, algae inadequate supply with essential nutrient, uncomfortable external abiotic conditions (aeration, moisture, pH, temperature), and low bioavailability. Bioremediation depending on several factors, which include but not limited to cost, site characteristics, type and concentration of pollutants. The leading step to a successful bioremediation is site description, which helps create the most suitable and promising bioremediation technique (ex-situ or in-situ).
Bioremediation uses microorganisms such as bacteria, fungi, and plants to degrade environmental pollutants into non-toxic matter. It can be used to treat contaminated soil and groundwater in situ (where it is found) or ex situ by removing the contaminated material and treating it elsewhere. There are various types of bioremediation depending on the technique used such as bioaugmentation, biostimulation, and phytoremediation. It is an effective and often low-cost approach to cleanup, but can be difficult to control and may not reduce contaminant concentrations to regulatory levels.
This document discusses bioremediation, which is the process of using microorganisms to break down hazardous contaminants in soil and water into nontoxic substances. It notes that rapid industrialization has contaminated the environment through emissions, spills, and effluents. Bioremediation can be used to naturally break down pollutants like petroleum hydrocarbons, chlorinated compounds, and nitroaromatics. There are two main types of bioremediation: in situ treats contaminants on-site, while ex situ involves removing and treating contaminated materials elsewhere. Technologies include bioventing, bioaugmentation, biosparging, land farming, composting, and bioreactors. Bioremediation has
A detailed presentation on current hot emerging topic BIOREMEDIATION explaining the process and the needs with advantages and disadvantages of the same
Applications of Environmental BiotechnologySamaunParvez1
This document provides an overview of various applications of environmental biotechnology including bioremediation, biomining, biomarkers, biodegradation, sewage treatment, biosorption, biofiltration, biosensors, and more. Environmental biotechnology uses biological processes like microorganisms and plants to solve environmental problems and protect ecosystems in a sustainable way. It summarizes key concepts and methods within each application area.
The document discusses selectable marker genes that are commonly used in plant transformation systems. Selectable marker genes are included in transformation vectors along with the target gene of interest. They confer resistance to transformed cells when grown on media containing toxic substances like antibiotics, herbicides, or antimetabolites. This allows transformed cells to survive while non-transformed cells die. There are three main categories of selectable marker genes: antibiotic resistance genes, antimetabolite marker genes, and herbicide resistance genes. Common examples of genes used include nptII for kanamycin resistance, pat/bar for phosphinothricin/glufosinate resistance, and epsps/aroA for glyphosate resistance.
This document discusses the essential components and formulation of microbial growth media. An ideal media provides nutrients for microbial growth and metabolite production while being non-toxic, avoiding foaming, and allowing for easy product recovery. Key components include a carbon source, nitrogen source, minerals, buffers, and sometimes precursors. Common carbon sources are saccharine materials like molasses, starchy materials, cellulosic wastes, and hydrocarbons. Media can be natural using agricultural byproducts or synthetic using purified compounds. Proper media formulation is important for successful experiments, processes, and economical production.
This document provides an overview of genome sequencing. It discusses that genome sequencing involves revealing the order of bases in an entire genome rather than sequencing genes one by one. Several methods of genome sequencing are described, including Sanger sequencing, automated sequencing, and ABE. Sanger sequencing was an early method that involved chain termination with dye-labeled dideoxynucleotides. Automated sequencing improved on this by running multiple reactions simultaneously in a single tube. Genome sequencing provides a wealth of genetic information and helps understand gene functions and interactions on a full genomic scale.
Lifestyle diseases are chronic non-communicable diseases that are primarily caused by modifiable behavioral risk factors like unhealthy diet, physical inactivity, tobacco and alcohol use. Some of the major lifestyle diseases include cardiovascular diseases, diabetes, cancer, and chronic respiratory diseases. Controlling behavioral risk factors through a healthy diet, exercise, avoiding tobacco and alcohol is key to preventing and managing lifestyle diseases. A comprehensive multi-sectoral approach involving healthcare, education, and policy can help minimize risk factors and ensure early detection and treatment of lifestyle diseases.
Lipofection is a chemical transfection method that uses liposomes to introduce nucleic acids into cells. Liposomes are lipid vesicles that can fuse with cell membranes and release their contents. In lipofection, nucleic acids bind to cationic liposomes to form lipoplexes, which are taken up by cells via endocytosis. Once inside endosomes, the lipoplexes destabilize the endosomal membranes through their cationic properties, allowing the nucleic acids to enter the cytoplasm and be expressed. Calcium chloride transformation is a common method for transforming competent bacterial cells with plasmid DNA. It involves treating cells with calcium chloride to increase membrane permeability, then exposing the cells to plasmid DNA and subjecting them to a heat shock to facilitate
Sickle cell anemia is a genetic blood disorder caused by a mutation in the beta-globin gene that results in abnormal hemoglobin. The red blood cells take on a sickle shape, which can cause them to block small blood vessels and obstruct blood flow. This document discusses the inheritance pattern, genetics, mechanism of sickling, signs and symptoms, complications, diagnosis, and treatment of sickle cell anemia. Treatment aims to prevent crises and complications through medications, blood transfusions, and potentially a bone marrow transplant to cure the disease.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...Sérgio Sacani
Context. With a mass exceeding several 104 M⊙ and a rich and dense population of massive stars, supermassive young star clusters
represent the most massive star-forming environment that is dominated by the feedback from massive stars and gravitational interactions
among stars.
Aims. In this paper we present the Extended Westerlund 1 and 2 Open Clusters Survey (EWOCS) project, which aims to investigate
the influence of the starburst environment on the formation of stars and planets, and on the evolution of both low and high mass stars.
The primary targets of this project are Westerlund 1 and 2, the closest supermassive star clusters to the Sun.
Methods. The project is based primarily on recent observations conducted with the Chandra and JWST observatories. Specifically,
the Chandra survey of Westerlund 1 consists of 36 new ACIS-I observations, nearly co-pointed, for a total exposure time of 1 Msec.
Additionally, we included 8 archival Chandra/ACIS-S observations. This paper presents the resulting catalog of X-ray sources within
and around Westerlund 1. Sources were detected by combining various existing methods, and photon extraction and source validation
were carried out using the ACIS-Extract software.
Results. The EWOCS X-ray catalog comprises 5963 validated sources out of the 9420 initially provided to ACIS-Extract, reaching a
photon flux threshold of approximately 2 × 10−8 photons cm−2
s
−1
. The X-ray sources exhibit a highly concentrated spatial distribution,
with 1075 sources located within the central 1 arcmin. We have successfully detected X-ray emissions from 126 out of the 166 known
massive stars of the cluster, and we have collected over 71 000 photons from the magnetar CXO J164710.20-455217.
When I was asked to give a companion lecture in support of ‘The Philosophy of Science’ (https://shorturl.at/4pUXz) I decided not to walk through the detail of the many methodologies in order of use. Instead, I chose to employ a long standing, and ongoing, scientific development as an exemplar. And so, I chose the ever evolving story of Thermodynamics as a scientific investigation at its best.
Conducted over a period of >200 years, Thermodynamics R&D, and application, benefitted from the highest levels of professionalism, collaboration, and technical thoroughness. New layers of application, methodology, and practice were made possible by the progressive advance of technology. In turn, this has seen measurement and modelling accuracy continually improved at a micro and macro level.
Perhaps most importantly, Thermodynamics rapidly became a primary tool in the advance of applied science/engineering/technology, spanning micro-tech, to aerospace and cosmology. I can think of no better a story to illustrate the breadth of scientific methodologies and applications at their best.
Travis Hills' Endeavors in Minnesota: Fostering Environmental and Economic Pr...Travis Hills MN
Travis Hills of Minnesota developed a method to convert waste into high-value dry fertilizer, significantly enriching soil quality. By providing farmers with a valuable resource derived from waste, Travis Hills helps enhance farm profitability while promoting environmental stewardship. Travis Hills' sustainable practices lead to cost savings and increased revenue for farmers by improving resource efficiency and reducing waste.
Current Ms word generated power point presentation covers major details about the micronuclei test. It's significance and assays to conduct it. It is used to detect the micronuclei formation inside the cells of nearly every multicellular organism. It's formation takes place during chromosomal sepration at metaphase.
The debris of the ‘last major merger’ is dynamically youngSérgio Sacani
The Milky Way’s (MW) inner stellar halo contains an [Fe/H]-rich component with highly eccentric orbits, often referred to as the
‘last major merger.’ Hypotheses for the origin of this component include Gaia-Sausage/Enceladus (GSE), where the progenitor
collided with the MW proto-disc 8–11 Gyr ago, and the Virgo Radial Merger (VRM), where the progenitor collided with the
MW disc within the last 3 Gyr. These two scenarios make different predictions about observable structure in local phase space,
because the morphology of debris depends on how long it has had to phase mix. The recently identified phase-space folds in Gaia
DR3 have positive caustic velocities, making them fundamentally different than the phase-mixed chevrons found in simulations
at late times. Roughly 20 per cent of the stars in the prograde local stellar halo are associated with the observed caustics. Based
on a simple phase-mixing model, the observed number of caustics are consistent with a merger that occurred 1–2 Gyr ago.
We also compare the observed phase-space distribution to FIRE-2 Latte simulations of GSE-like mergers, using a quantitative
measurement of phase mixing (2D causticality). The observed local phase-space distribution best matches the simulated data
1–2 Gyr after collision, and certainly not later than 3 Gyr. This is further evidence that the progenitor of the ‘last major merger’
did not collide with the MW proto-disc at early times, as is thought for the GSE, but instead collided with the MW disc within
the last few Gyr, consistent with the body of work surrounding the VRM.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
ANAMOLOUS SECONDARY GROWTH IN DICOT ROOTS.pptxRASHMI M G
Abnormal or anomalous secondary growth in plants. It defines secondary growth as an increase in plant girth due to vascular cambium or cork cambium. Anomalous secondary growth does not follow the normal pattern of a single vascular cambium producing xylem internally and phloem externally.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
The binding of cosmological structures by massless topological defectsSérgio Sacani
Assuming spherical symmetry and weak field, it is shown that if one solves the Poisson equation or the Einstein field
equations sourced by a topological defect, i.e. a singularity of a very specific form, the result is a localized gravitational
field capable of driving flat rotation (i.e. Keplerian circular orbits at a constant speed for all radii) of test masses on a thin
spherical shell without any underlying mass. Moreover, a large-scale structure which exploits this solution by assembling
concentrically a number of such topological defects can establish a flat stellar or galactic rotation curve, and can also deflect
light in the same manner as an equipotential (isothermal) sphere. Thus, the need for dark matter or modified gravity theory is
mitigated, at least in part.
BREEDING METHODS FOR DISEASE RESISTANCE.pptxRASHMI M G
Plant breeding for disease resistance is a strategy to reduce crop losses caused by disease. Plants have an innate immune system that allows them to recognize pathogens and provide resistance. However, breeding for long-lasting resistance often involves combining multiple resistance genes
3. Bioremediation
Defined as,
Bioremediation is a means of cleaning up
contaminated environments by exploiting the diverse
metabolic abilities of microorganisms to convert
contaminants to harmless products by
mineralization, generation of carbon dioxide and
water, or by conversion into microbial biomass
4. Principle
• It's main role is to return polluted environments to
their natural state.
• The pollutants that can be sequestered or removed
by microorganisms include toxic heavy metals such
as lead, cadmium, arsenic and radioactive metals.
5. Cont'd....
In general, Bioremediation methodologies focus on-
1. Enhancing the abundance of certain species or
groups of microorganisms that can metabolize toxic
chemicals (Bioagumentation)
2. Optimizing environmental conditions for the actions
of these organisms. It focuses on rapidly increasing the
abundance of naturally occurring ubiquitous
microorganisms.
6. Types of Bioremediation
On the basis of place where wastes are removed,
there are principally two ways of bioremediation:
1. Insitu bioremediation
2. Exsitu bioremediation
7.
8. Insitu bioremediation
• Treating polluted substance at the site of pollution
• Doesn't require excavation
• This technique is less expensive
• Intrinsic or natural bioremediation is a process
where no enhancement is require ment
• It can be used to treat successfully chlorinated
solvents, dyes, heavy metal & hydrocarbon
9. Two types of in situ bioremediation are distinguished
based on the origin of the microorganisms applied as
bioremediants:
(i) Intrinsic bioremediation-
• Is carried out without direct microbial amendment
• Through intermediation in ecological conditions of
the contaminated region
• Fortification of the natural populations and the
metabolic activities of indigenous or naturally existing
microfauna by improving nutritional and ventilation
conditions.
10. (ii) Engineered in situ bioremediation—
• Is performed through the introduction of certain
microorgansims to contamination site.
• The conditions of contamination sites are most
often unfavorable for the establishment and
bioactivity of the exogenously amended
microorganisms
• therefore, the environment is modified in a way
that improved physico-chemical conditions are
provided.
11. Extrinsic bioremediation
• It is done somewhere out from Contaminated site
i.e., excavating contaminated soil or pumping out the
ground water to sit of bioremediation
• Techniques based on - the cost of treatment ;depth
of pollution ;geology of polluted site.
• Depending on the state of the contaminant in the
step of bioremediation, ex situ bioremediation is
classified as:
12. • Solid phase system —The system is used in order to
bioremediate organic wastes and problematic
domestic and industrial wastes, sewage sludge, and
municipal solid wastes.
• Slurry phase systems - Slurry phase
bioremediation is a relatively more rapid process
compared to the other treatment processes.
14. Bioventing
It's a process of stimulating the natural
onsite bioremediation of contaminants in
soul by providing air or oxygen to existing
soil microorganisms.
Biovenrung useslow air flow rates to
provide oxygen to sustain mictibial
activity in the vadise zone
This technique is used in restoring sites
pollutted with Petroleum products to bit
light
15. Biosparging
• involves injection of air
under pressure below the
water level to increase
ground water concentration
• To enhance rate of biological
degradation of contaminants
by naturally occurring
bacteria
16. Biopile
• Are hybrid of land n composting
• Typically used for treatment of surface
contaminants
• It provides a favorable environment for indigenous
aerobic and anaerobic microorganisms
17.
18. Land farming
• Is a simple technique
• Here contaminated soil is excavated and spread
over a bed prepared bed and periodically tilled until
pollutants are degraded
• The practice is limited to treatment of superficial 10
- 35cm of soil
19.
20. Phytoremediation remediation
• Phytoremediation is a technology that uses plants
to degrade, assimilate, metabolize, or detoxify
metal and organic chemical contamination.
• Phytoremediation can provide a low-cost and
sustainable way to improve the economies of
developing countries.
21.
22. • Phytoremediation basically refers to the use of
plants and associated soil microbes to reduce the
concentrations or toxic effects of contaminants in
the environment. Phytoremediation is widely
accepted as a cost-effective environmental
restoration technology.