4. Genetic recombination
• Genetic variability – ESSENTIAL!!!
• Survival of the species
• Diploid Eukaryotes
• Crossing over
• Meiosis
• Haploid Prokaryotes
• Transduction
• Transformation
• Conjugation
5. Transduction
• Genetic material exchanged via Viruses
• E.g. bacteriophage lambda
• No physical contact b/w bacteria
• Promotes drug resistance
• Horizontal gene transfer
6. Transformation
• Acquisition of exogenous DNA fragments from environment
• E.g. Plasmids
• Antibiotic resistance
• Pesticide resistance in crops
• E.g. Agrobacterium tumefaciens
• Horizontal gene transfer
7. Conjugation
• Exchange of genetic material b/w bacteria
• Physical contact required
• DNA exchanged via Pilli
• Drug resistance!!
• Horizontal Gene Transfer
8. Conjugation
Bacteria A Bacteria B
Transfer of genetic material from Bacteria A to Bacteria B by matting or
contact is called conjugation
9. Who Is Donor
F Plasmid Absent
Donor recipient
F+ F-
Male cell Female cell
12. Terminology
The Donor:- F+ , Fertility factor - Contains genetic material for gene
exchange “male” cell
The Recipient:- F- No fertility factor Receives genetic material
“Female” cell
The Conjugate:- HFR -High frequency recombinant , Resulting
genetic recombinant ,Transfers entire genomic DNA when mixed with F-
cells
13. 1. Cell – Cell Contact
Formation of “sex pilus” or F-bridge between cells