This document discusses bacterial transformation, which is the process of transferring foreign DNA into bacterial host cells. It describes how the pGLO plasmid containing GFP and beta-lactamase genes is used to transform E. coli HB101 host cells. The process involves cutting the plasmid with restriction enzymes, joining it with DNA fragments using DNA ligase, making the host cells competent, and selecting transformed cells on antibiotic-containing media. GFP is used as a reporter to identify transformed cells expressing the plasmid genes.