Bacterial conjugation involves the transfer of genetic material between bacteria through direct contact. It was first discovered in 1946 by Lederberg and Tatum in E. coli K12 strains. The process involves a donor bacterium containing an F plasmid transferring it to a recipient bacterium. The F plasmid encodes for sex pili that allow the transfer of genetic material. During conjugation, the recipient bacterium receives part of the donor's DNA and may gain traits like antibiotic resistance.

1. MICROBIOLOGY
CONJUGATION
Meghna V
III Semester
MGMCH

2. BACTERIAL CONJUGATION WAS FIRST
DISCOVERED BY
Lederberg And Tatum
IN 1946
IN Esch.coliK12 STRAINS

3. CONJUGATION
BACTERIA A
BACTERIA B
TRANSFER OF GENETIC MATERIAL FROM BACTERIA A
TO BACTERIA B BY MATING OR CONTACT IS CALLED
CONJUGATION.

4. WHO IS A DONOR?
F PLASMID
DONOR
F+
MALE CELL
ABSENT
RECIPIENT
F–
FEMALE

5. F PLASMID
FERTILITY FACTOR
SEX FACTOR
TRANSFER FACTOR

6. THIS F PLASMID ENCODES FOR THE SEX PILUS

7. WHAT HAPPENS DURING
CONJUGATION?

8. THE ONCE FEMALE RECIPIENT CELL NOW
BECOMES THE MALE DONOR CELL
THIS CHARACTERISTIC OF MALENESS (F+) IN
BACTERIA IS TRANSMISSIBLE OR
INFECTIOUS

9. What is Hfr cell?
When F factor/ plasmid exists in
an integrated state with the host
chromosome

10. Hfr CONJUGATION

11. F+ (free plasmid)
Hfr ( integrated )
REVERSIBLE
TURNS A (F-)
INTO A (F+) UPON
CONJUGATION
CONJUGATION WITH A
Hfr , AN (F-) RARELY
BECOMES (F+) BUT IT
RECEIVES CHROMOSOMAL
DNA FROM THE
DONOR

12. REVERSIBLE
(F+)
Hfr

13. SEXDUCTION

14. F+
Hfr
REVERSIBLE
WHEN THE F FACTOR REVERTS FROM
INTEGRATED
FREE STATE IT MAY
SOMETIMES CARRY WITH IT SOME
CHROMOSOMAL DNA FROM ADJACENT SITE OF
ITS ATTACHMENT
SUCH AN F FACTOR IS KNOWN AS F’ FACTOR

15. ( F’) + ( F-)
SEXDUCTION
WHEN (F’) CONJUGATES WITH A RECIPIENT (F), IT TRANSFERS, ALONG WITH THE F
FACTOR, THE HOST DNA INCORPORATED
WITH IT.

16. MEDICALLY IMPORTANT FACTORS
WHICH CAN BE TRANSFERRED BY
CONJUGATION
1. COLICOGENIC FACTOR (COL)
2. RESISTANCE TRANSFER FACTOR (RTF)

17. THANKYOU