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MICROBIOLOGY
CONJUGATION
Meghna V
III Semester
MGMCH
BACTERIAL CONJUGATION WAS FIRST
DISCOVERED BY
Lederberg And Tatum
IN 1946
IN Esch.coliK12 STRAINS
CONJUGATION
BACTERIA A

BACTERIA B

TRANSFER OF GENETIC MATERIAL FROM BACTERIA A
TO BACTERIA B BY MATING OR CONTACT IS CALLED
CONJUGATION.
WHO IS A DONOR?

F PLASMID
DONOR
F+
MALE CELL

ABSENT
RECIPIENT
F–
FEMALE
F PLASMID
FERTILITY FACTOR
SEX FACTOR
TRANSFER FACTOR
THIS F PLASMID ENCODES FOR THE SEX PILUS
WHAT HAPPENS DURING
CONJUGATION?
THE ONCE FEMALE RECIPIENT CELL NOW
BECOMES THE MALE DONOR CELL

THIS CHARACTERISTIC OF MALENESS (F+) IN
BACTERIA IS TRANSMISSIBLE OR
INFECTIOUS
What is Hfr cell?
When F factor/ plasmid exists in
an integrated state with the host
chromosome
Hfr CONJUGATION
F+ (free plasmid)

Hfr ( integrated )

REVERSIBLE

TURNS A (F-)
INTO A (F+) UPON
CONJUGATION

CONJUGATION WITH A
Hfr , AN (F-) RARELY
BECOMES (F+) BUT IT
RECEIVES CHROMOSOMAL
DNA FROM THE
DONOR
REVERSIBLE

(F+)

Hfr
SEXDUCTION
F+

Hfr
REVERSIBLE

WHEN THE F FACTOR REVERTS FROM
INTEGRATED
FREE STATE IT MAY
SOMETIMES CARRY WITH IT SOME
CHROMOSOMAL DNA FROM ADJACENT SITE OF
ITS ATTACHMENT

SUCH AN F FACTOR IS KNOWN AS F’ FACTOR
( F’) + ( F-)

SEXDUCTION

WHEN (F’) CONJUGATES WITH A RECIPIENT (F), IT TRANSFERS, ALONG WITH THE F
FACTOR, THE HOST DNA INCORPORATED
WITH IT.
MEDICALLY IMPORTANT FACTORS
WHICH CAN BE TRANSFERRED BY
CONJUGATION
1. COLICOGENIC FACTOR (COL)
2. RESISTANCE TRANSFER FACTOR (RTF)
THANKYOU

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Conjugation Microbiology powerpoint presentation