This document summarizes the synthesis, characterization, and in-vitro antimicrobial studies of a copper(II) complex derived from black pepper. Specifically, it details the isolation of 1-[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]piperidine from black pepper through solvent extraction. It then describes the synthesis of the copper(II) complex using the isolated compound as a ligand. Various spectroscopic techniques including FT-IR, NMR, and UV-Vis were used to characterize the ligand and complex. Finally, the antimicrobial activity of both the ligand and complex were evaluated against various bacteria and fungi, finding that the copper
ABSTRACT- The aim of this study was to investigate in vitro antioxidant activity and anti-bacterial activity of the petroleum ether, ethyl acetate
and methanol extract obtained from the whole part of Jurinea dolomiaea Boiss (Asteraceae). Total phenolic and flavonoid contents of these extracts
were determined as gallic acid and rutin equivalents, respectively. Total antioxidant activity, reducing power of these extract were evaluated as ascorbic
acid and gallic acid equivalents, respectively. ABTS free radical scavenging activity is expressed as trolox equivalent antioxidant capacity
(TEAC). The antibacterial activity of the extract was investigated by disc diffusion method. The ethyl acetate and methanol extracts showed moderate
activity against E. coli and S. aureus.
Key words: Jurinea dolomiaea; Total phenolic; Total flavonoid; Total antioxidant; Free radical scavenging activity; Antibacterial activity.
Effects of roasting on the total phenolic contents and radical scavenging act...Innspub Net
The purpose of the present study was to explore the influences roasting on the radical scavenging activity and total Phenolic content on selected seeds. Fresh seeds of Prunus domestice, Prunus armeniace and Prunus persica were selected from the local market. The selected seeds were heated on the hotpot at a temperature 160 °C for 1 to 3 hours, respectively and one group were remain irrespective of any treatment (control). It was observed that roasting of fruit seeds produce different effects on total phenolic contents and radical scavenging activity. Antioxidant capacity was measured against the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) whereas the reducing capacity was evaluated with the Folin-Ciocalteu reagent (FCR). Total phenolic content in Prunus domestica was highest at 160 °C when heated for 1 hour (554 mg/100g), similarly the total phenolic content in the Prunus armeniaca was highest when heated for 2 hour (684 mg/100g) while the Total phenolic content in the Prunus persica was highest when heated for 2 hour (684 mg/100g). Radical scavenging activity in the Prunus domestica was highest when heated for 1 hour (48 %). Similarly radical scavenging activity in the Prunus armeniaca was highest during heated for 1 hour (86 %) while radical scavenging activity in the Prunus persica was at maximum (43 %) at 2 hour treatment. It is suggested that different effect were produced when different fruit seeds were roasted at a single temperature, Therefore different optimum temperature and conditions are required for roasting different seeds.
Synthesis and Pharmacological evaluation of new Benzoxozole DerivativesSriramNagarajan15
Benzoxazoles1 are usually prepared by heating 2-Aminophenol with formic acids in the presence of Boric acid under reflux. Condensation of these two substances under milder conditions. Being a heterocyclic compound, benzoxazole finds use in research as a starting material for the synthesis of larger, usually bioactive structures. It is found within the chemical structures of pharmaceutical drugs such as flunoxaprofen. Benzoxazole derivatives are provided a protection against noxious UV radiation.Benzoxazole derivatives are also used in cosmotic compositions, such as for examples mainly cinnamic acid, 4-aminobenzoic acid.Benzoxazole derivatives are also used in the optical brighteners.These derivatives are used as Anticonvulsant and Neurotoxicity2 , Anti-inflammatory agents3, “Antibacterial activity”4, Cholesteryl ester transfer Protein inhibitors5, Antimicrobial activity6,7, Antifungal activity’8, Cyclooxygenase inhibitors9, hair treatment products and also used as a skin protectants.
ABSTRACT- The aim of this study was to investigate in vitro antioxidant activity and anti-bacterial activity of the petroleum ether, ethyl acetate
and methanol extract obtained from the whole part of Jurinea dolomiaea Boiss (Asteraceae). Total phenolic and flavonoid contents of these extracts
were determined as gallic acid and rutin equivalents, respectively. Total antioxidant activity, reducing power of these extract were evaluated as ascorbic
acid and gallic acid equivalents, respectively. ABTS free radical scavenging activity is expressed as trolox equivalent antioxidant capacity
(TEAC). The antibacterial activity of the extract was investigated by disc diffusion method. The ethyl acetate and methanol extracts showed moderate
activity against E. coli and S. aureus.
Key words: Jurinea dolomiaea; Total phenolic; Total flavonoid; Total antioxidant; Free radical scavenging activity; Antibacterial activity.
Effects of roasting on the total phenolic contents and radical scavenging act...Innspub Net
The purpose of the present study was to explore the influences roasting on the radical scavenging activity and total Phenolic content on selected seeds. Fresh seeds of Prunus domestice, Prunus armeniace and Prunus persica were selected from the local market. The selected seeds were heated on the hotpot at a temperature 160 °C for 1 to 3 hours, respectively and one group were remain irrespective of any treatment (control). It was observed that roasting of fruit seeds produce different effects on total phenolic contents and radical scavenging activity. Antioxidant capacity was measured against the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) whereas the reducing capacity was evaluated with the Folin-Ciocalteu reagent (FCR). Total phenolic content in Prunus domestica was highest at 160 °C when heated for 1 hour (554 mg/100g), similarly the total phenolic content in the Prunus armeniaca was highest when heated for 2 hour (684 mg/100g) while the Total phenolic content in the Prunus persica was highest when heated for 2 hour (684 mg/100g). Radical scavenging activity in the Prunus domestica was highest when heated for 1 hour (48 %). Similarly radical scavenging activity in the Prunus armeniaca was highest during heated for 1 hour (86 %) while radical scavenging activity in the Prunus persica was at maximum (43 %) at 2 hour treatment. It is suggested that different effect were produced when different fruit seeds were roasted at a single temperature, Therefore different optimum temperature and conditions are required for roasting different seeds.
Synthesis and Pharmacological evaluation of new Benzoxozole DerivativesSriramNagarajan15
Benzoxazoles1 are usually prepared by heating 2-Aminophenol with formic acids in the presence of Boric acid under reflux. Condensation of these two substances under milder conditions. Being a heterocyclic compound, benzoxazole finds use in research as a starting material for the synthesis of larger, usually bioactive structures. It is found within the chemical structures of pharmaceutical drugs such as flunoxaprofen. Benzoxazole derivatives are provided a protection against noxious UV radiation.Benzoxazole derivatives are also used in cosmotic compositions, such as for examples mainly cinnamic acid, 4-aminobenzoic acid.Benzoxazole derivatives are also used in the optical brighteners.These derivatives are used as Anticonvulsant and Neurotoxicity2 , Anti-inflammatory agents3, “Antibacterial activity”4, Cholesteryl ester transfer Protein inhibitors5, Antimicrobial activity6,7, Antifungal activity’8, Cyclooxygenase inhibitors9, hair treatment products and also used as a skin protectants.
PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF VARIOUS SOLVENT EXTRAC...IJSIT Editor
The leaves of the plant Annonareticulata were collected and extracted using different ranges of polar
organic solvents like low (Ethyl acetate), medium (Butanol) and high (Methanol). Qualitative analysis and
antimicrobial activity was investigated. The phytochemical screening of the leaf extract revealed that the
presence of alkaloids, tannins, steroids, terpenoids and coumarins. The Ethyl acetate and Methanol extracts
showed better antibacterial activity, the significant inhibitory effect against Escherichia coli, Pseudomonas
putida and Lactobacillus acidophilus, and thus displayed highest inhibitory zone of 19.5mm, 19mm and 19mm
when compared to Butanol. FT-IR spectroscopic analysis of the Ethyl acetate, Butanol and Methanol extract of
A.reticulata revealed the presence of -CH, -OH, CH-OH and –NH2 bond stretching. The clinical isolates were
collected from patients suffered from different microbial infections. The antibacterial and antifungal activity
was determined by using leaf extracts.
Antimicrobial and antioxidant activities of the essentialChina
Antimicrobial and Antioxidant Activities of the Essential Oils of Some Aromatic Medicinal Plants (Pulicaria inuloides-Asteraceae and Ocimum forskolei-Lamiaceae)
Phytochemical screening and in vitro antioxidant activity of extracts of jasm...SriramNagarajan16
Objectives
The aims of this research were to carry out the preliminary phytochemical screening and antioxidant activity
of different extracts of J. sessiliflorum. The different anti-oxidant methods carried out were DPPH
scavenging method, NBT dye reduction method and nitric oxide scavenging method
Methods
Extracts were prepared by reflux method using different polarity solvents. The extracts were evap orated
using rotary evaporator. Antioxidant activities using DPPH, NBT dye reduction method and nitric oxide
scavenging methods and the correlation of their IC50 values with standards were carried out.
Results
The ethanolic herbs extract of J. sessiliflorum had the lowest IC50 values in all the anti-oxidant methods.
Moreover, the ethanolic extracts showed the presence greatest amount of phytochemical constituents. The
IC50 values were correlated with the IC50 values of standards in all the anti- oxidant activity determination
methods.
Conclusions
The results of the present study indicate that the extracts of J.sessiliflorum exhibited strong antioxidant
activity and thus it is a good source of antioxidant.
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
Annona squamosa as botanical pesticide;
Annonaceous acetogenins; annonin/squamocin;
Advancement in botanical research;
Structure activity relationship of squamocin/annonin; Chemistry and mode of action of squamocin/annonin
Isolation and Pharmacological Activities of Curcumin from Curcuma longa L.AnuragSingh1049
Curcuma longa commonly known as turmeric belongs to the family Zingiberaceae. It is mainly used for treating chronic diseases especially Diabetes mellitus. The present study aims at evaluating the anti-oxidant, anti-haemolytic, anti-angiogenesis and anti-bacterial activities of Curcumin fraction. Different antioxidant assays were carried out for evaluating the antioxidant capacity of aqueous extract of Curcuma longa. The maximum1, 1- diphenyl 2-picrylhydrazyl (DPPH) radical scavenging activity of curcumin fraction was 30.62±0.87 % at 60 µg/mL concentration. The inhibitory concentration (IC50) values of DPPH˙ radical scavenging activity was 97.97 µg/mL concentration. The maximum superoxide radical scavenging activity of Curcuma longa extract was 57.01±0.17 % at 60 µg/mL concentration and the inhibitory concentration (IC50) was 46.91 µg/mL concentration. The maximum anti-haemolytic activity of curcumin fraction was 44.53±0.84 and the IC50 was 67.37 µg/mL. The maximum Fe3+ reduction and Mo6+ reduction were 54.85±0.35 % and 61.13±0.21 % at 60 µg/mL concentration and the reduction concentration (RC50) were 51.59 µg/mL and 28.69 µg/mL concentration respectively.
ABSTRACT
Azadirachta indica common name Neem is a very useful traditional medicinal plant in the sub-continent and each part of the tree has some medicinal properties. It has many therapeutic properties as it boosts the immune system, beneficial in treating acne, provides relief from bad breath, helps to protect against diabetes, effective in treating malaria symptoms, lowers the risk of cancer and cardiovascular disease. The plant is native to Asia and its sub-continents. Therefore, the aim of the present study was to investigate the phytochemical constituents present in leaves and bark of neem. The antioxidant activity, total phenolic, and flavonoid contents were also evaluated. Neem leaves and bark were collected from the Bundelkhand University Campus, Jhansi. It was cleaned with tap water and finally with distilled water and was air dried at room temperature and crushed. TLC was done for the determination of bioactive compounds present in the leaves. A qualitative phytochemical analysis was performed and we found that alkaloids, reducing sugar, flavonoids, glycosides, tannins, phenolic compounds, saponins are present in neem leaves and bark. TLC results shown total 8 spots in the methanolic leaves extract having different Rf values. The total antioxidant capacity of A. indica leaves shown the dose dependent activities. The mean values of total phenolic contents and flavonoids are 70 mg GAE/g & 119 mg QE/g respectively. Thus in the present study, the presence of flavonoids and phenolic compounds in the neem leaves extract could be considered responsible for conferring antioxidant ability.
Key-words: Phytochemical, Azadirachta indica, Antioxidant Activity, Total Phenolic Content (TPC), Total Flavonoids Content (TFC)
Catalysis of SO2 to SO3 by Ocimum Sanctum Mediated Potassium Vanadate (KV2O5)...Arvind Singh Heer
Eco friendly and cost effective methods of green mediated synthesis of nanoparticles are the present research in the limb of nanotechnology. The present work leads to the synthesis of potassium-deficient (KV2O5) nanocuboid from 0.008 moles of Ammonium Meta-Vanadate and 0.002 mole of Potassium Oxalate added to aqueous leaf extract of Ocimum Sanctum which acts as reducing as well as capping agent. The characteristics of Potassium Vanadate nanoparticles were studied by using UV-VIS spectroscopy, XRD, FTIR, SEM and EDS. Ultraviolet scanning spectroscopy was used to detect the distinct absorption spectrum of KV2O5 nanoparticles. The peak value observed at 345 nm. The EDS spectrum of the KV2O5 nanoparticles confirmed the presence of elemental Potassium and Vanadium and pentavalent Vanadium was also determined by EDS in its oxide form. The size of synthesized nanocuboid lies between 50-200 nm. The catalytic activity of prepared nanoparticles was studied for the oxidation of SO2 to SO3.
PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF VARIOUS SOLVENT EXTRAC...IJSIT Editor
The leaves of the plant Annonareticulata were collected and extracted using different ranges of polar
organic solvents like low (Ethyl acetate), medium (Butanol) and high (Methanol). Qualitative analysis and
antimicrobial activity was investigated. The phytochemical screening of the leaf extract revealed that the
presence of alkaloids, tannins, steroids, terpenoids and coumarins. The Ethyl acetate and Methanol extracts
showed better antibacterial activity, the significant inhibitory effect against Escherichia coli, Pseudomonas
putida and Lactobacillus acidophilus, and thus displayed highest inhibitory zone of 19.5mm, 19mm and 19mm
when compared to Butanol. FT-IR spectroscopic analysis of the Ethyl acetate, Butanol and Methanol extract of
A.reticulata revealed the presence of -CH, -OH, CH-OH and –NH2 bond stretching. The clinical isolates were
collected from patients suffered from different microbial infections. The antibacterial and antifungal activity
was determined by using leaf extracts.
Antimicrobial and antioxidant activities of the essentialChina
Antimicrobial and Antioxidant Activities of the Essential Oils of Some Aromatic Medicinal Plants (Pulicaria inuloides-Asteraceae and Ocimum forskolei-Lamiaceae)
Phytochemical screening and in vitro antioxidant activity of extracts of jasm...SriramNagarajan16
Objectives
The aims of this research were to carry out the preliminary phytochemical screening and antioxidant activity
of different extracts of J. sessiliflorum. The different anti-oxidant methods carried out were DPPH
scavenging method, NBT dye reduction method and nitric oxide scavenging method
Methods
Extracts were prepared by reflux method using different polarity solvents. The extracts were evap orated
using rotary evaporator. Antioxidant activities using DPPH, NBT dye reduction method and nitric oxide
scavenging methods and the correlation of their IC50 values with standards were carried out.
Results
The ethanolic herbs extract of J. sessiliflorum had the lowest IC50 values in all the anti-oxidant methods.
Moreover, the ethanolic extracts showed the presence greatest amount of phytochemical constituents. The
IC50 values were correlated with the IC50 values of standards in all the anti- oxidant activity determination
methods.
Conclusions
The results of the present study indicate that the extracts of J.sessiliflorum exhibited strong antioxidant
activity and thus it is a good source of antioxidant.
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
Annona squamosa as botanical pesticide;
Annonaceous acetogenins; annonin/squamocin;
Advancement in botanical research;
Structure activity relationship of squamocin/annonin; Chemistry and mode of action of squamocin/annonin
Isolation and Pharmacological Activities of Curcumin from Curcuma longa L.AnuragSingh1049
Curcuma longa commonly known as turmeric belongs to the family Zingiberaceae. It is mainly used for treating chronic diseases especially Diabetes mellitus. The present study aims at evaluating the anti-oxidant, anti-haemolytic, anti-angiogenesis and anti-bacterial activities of Curcumin fraction. Different antioxidant assays were carried out for evaluating the antioxidant capacity of aqueous extract of Curcuma longa. The maximum1, 1- diphenyl 2-picrylhydrazyl (DPPH) radical scavenging activity of curcumin fraction was 30.62±0.87 % at 60 µg/mL concentration. The inhibitory concentration (IC50) values of DPPH˙ radical scavenging activity was 97.97 µg/mL concentration. The maximum superoxide radical scavenging activity of Curcuma longa extract was 57.01±0.17 % at 60 µg/mL concentration and the inhibitory concentration (IC50) was 46.91 µg/mL concentration. The maximum anti-haemolytic activity of curcumin fraction was 44.53±0.84 and the IC50 was 67.37 µg/mL. The maximum Fe3+ reduction and Mo6+ reduction were 54.85±0.35 % and 61.13±0.21 % at 60 µg/mL concentration and the reduction concentration (RC50) were 51.59 µg/mL and 28.69 µg/mL concentration respectively.
ABSTRACT
Azadirachta indica common name Neem is a very useful traditional medicinal plant in the sub-continent and each part of the tree has some medicinal properties. It has many therapeutic properties as it boosts the immune system, beneficial in treating acne, provides relief from bad breath, helps to protect against diabetes, effective in treating malaria symptoms, lowers the risk of cancer and cardiovascular disease. The plant is native to Asia and its sub-continents. Therefore, the aim of the present study was to investigate the phytochemical constituents present in leaves and bark of neem. The antioxidant activity, total phenolic, and flavonoid contents were also evaluated. Neem leaves and bark were collected from the Bundelkhand University Campus, Jhansi. It was cleaned with tap water and finally with distilled water and was air dried at room temperature and crushed. TLC was done for the determination of bioactive compounds present in the leaves. A qualitative phytochemical analysis was performed and we found that alkaloids, reducing sugar, flavonoids, glycosides, tannins, phenolic compounds, saponins are present in neem leaves and bark. TLC results shown total 8 spots in the methanolic leaves extract having different Rf values. The total antioxidant capacity of A. indica leaves shown the dose dependent activities. The mean values of total phenolic contents and flavonoids are 70 mg GAE/g & 119 mg QE/g respectively. Thus in the present study, the presence of flavonoids and phenolic compounds in the neem leaves extract could be considered responsible for conferring antioxidant ability.
Key-words: Phytochemical, Azadirachta indica, Antioxidant Activity, Total Phenolic Content (TPC), Total Flavonoids Content (TFC)
Catalysis of SO2 to SO3 by Ocimum Sanctum Mediated Potassium Vanadate (KV2O5)...Arvind Singh Heer
Eco friendly and cost effective methods of green mediated synthesis of nanoparticles are the present research in the limb of nanotechnology. The present work leads to the synthesis of potassium-deficient (KV2O5) nanocuboid from 0.008 moles of Ammonium Meta-Vanadate and 0.002 mole of Potassium Oxalate added to aqueous leaf extract of Ocimum Sanctum which acts as reducing as well as capping agent. The characteristics of Potassium Vanadate nanoparticles were studied by using UV-VIS spectroscopy, XRD, FTIR, SEM and EDS. Ultraviolet scanning spectroscopy was used to detect the distinct absorption spectrum of KV2O5 nanoparticles. The peak value observed at 345 nm. The EDS spectrum of the KV2O5 nanoparticles confirmed the presence of elemental Potassium and Vanadium and pentavalent Vanadium was also determined by EDS in its oxide form. The size of synthesized nanocuboid lies between 50-200 nm. The catalytic activity of prepared nanoparticles was studied for the oxidation of SO2 to SO3.
QQM4 new portable device for Quick Yarn Quality Measuring
QQM4 is the unique portable equipment designed for a direct and quick quality control of yarn in
process – no interruption or any adjustment of yarn is needed. It enables to analyse yarn quality very
quickly and directly on the machine in operation.
QQM technology is based on optical principle and its application in spinning process eliminates defects
visible after weaving and dyeing (e.g. moiré effect, naps).
• Jaonori fiber of karagenan, which carragenan alone have been tested clinically show result of seething with excitement to blood lipid profile control especially cholesterol, HDL and triglyceride.
• Jaonori represent fiber of carragenan which originate from seaweed of Euchema cottoni have benefit like :
a. Controlling cholesterol
b. Preventing coronary heart disease and of stroke
c. Overcoming constipation
d. Preventing haemorrhoid
e. Preventing large bowel cancer
f. Controlling body weight
g. Preventing fatness
Lessons learnt from CIFOR research for PFES in VietnamCIFOR-ICRAF
This presentation was given by Pham Thu Thuy on the “Regional workshop on Payment for Environmental Services” on November 19 2014 in Hanoi, Vietnam. The overall aim of the workshop was to enhance the understanding and capacity of policy makers, PES practioners, and researcher communities on the topic of payments for ecosystem services and ecosystem-based approaches and also to increase dialogue between them on latest lessons learned and recommendations for effective, efficient and equitable implementation of PES.
Antibacterial Property of Essential Oil and its vibrational analysisIOSR Journals
In recent years there has been an increasing interest in the use of natural substances, and some questions concerning the safety of synthetic compounds have encouraged more detailed studies of plant resources. Essential oils, odorous and volatile products of plant secondary metabolism, have a wide application in folk medicine, food flavoring and preservation as well as in fragrance industries. In our present work with the vibrational spectroscopy of quantum chemical calculations of Eugenol,Geronial have been carried out. The compounds are responsible for most of the characteristic aroma .DFT analysis is performed before and after application of clove oil on Asbergillus fungi..Vibrational analysis on Eugenol( 4-allyl-2-methoxyphenol is also carried out.The fluorescent property of the material was also discussed.
In-vitro antioxidant and GC-MS analysis ethanolic extract of poly herbal drugSkyfox Publishing Group
Antioxidants play an important role in inhibiting and scavenging free radicals, thus providing protection to human against
infections and degenerative diseases. Current research is now directed towards natural antioxidants originated from plants due to safe
therapeutics. Poly herbal drugs is used in Indian traditional medicine for a wide range of various ailments. To understand the mechanism
of pharmacological actions, antioxidant properties of the Poly herbal drugs extract were tested using standard in vitro models. The
ethanolic extract of Poly herbal drugs exhibited strong scavenging effect on superoxide, nitric oxide radical and reducing power radical
scavenging assay. The free radical scavenging effect of Poly herbal drugs extract was comparable with that of the reference antioxidants.
The data obtained in the present study suggests that the extract of Poly herbal drugs have potent Invitro antioxidant and Anti Diabetic
activity against free radicals, prevent oxidative damage to major biomolecules and afford significant protection against oxidative damage.
Synthesis, Characterization, Biological Evaluation of Some Heterocyclic Oxaze...JohnJulie1
The new serious of pentyloxy and aryloxy benzaldehyde (3ae) were synthesized from the substitution reaction of 4-hydroxy benzaldehyde with different aryl bromide and alkyl bromide, on the basis of Williamson ether synthesis in the presence of Potassium carbonate using absolute ethanol as a solvent. The second step was the synthesis of 2-amino-5-(p-tolyl)1,3,4-thiadiazole.
Synthesis, Characterization, Biological Evaluation of Some Heterocyclic Oxaze...JapaneseJournalofGas
The new serious of pentyloxy and aryloxy benzaldehyde (3ae) were synthesized from the substitution reaction of 4-hydroxy benzaldehyde with different aryl bromide and alkyl bromide, on the basis of Williamson ether synthesis in the presence of Potassium carbonate using absolute ethanol as a solvent. The second step was the synthesis of 2-amino-5-(p-tolyl)1,3,4-thiadiazole.
Evaluation of Anti-oxidant Activity of Elytraria acaulis Aerial ExtractsIJERA Editor
Elytraria acaulis, a stem less perennial herb of Acantheceae family has many medicinal and therapeutic properties. Anti oxidative activity of the aerial parts of this Elytraria acaulis were assessed in the present study. The aerial parts of the plant (Stem & Leaves) were extracted in different organic solvents such as n-Hexane, Ethanol, Methanol, Ethyl Acetate and Chloroform. Initially, Total Phenolic & Total Flavonoids content in different solvent plant extracts were estimated. The free radical scavenging and antioxidant activity of the Elytraria acaulis aerial extracts in different organic solvents were also assayed by DPPH assay, FRAP assay. The aerial extracts of Elytraria acaulis have shown significant anti oxidant activity. Hence, further studies on this plant will enable elucidation of its therapeutic properties and medicinal applications
Phytochemical Screening, Antioxidant, and Antibacterial Activity of Dioon spi...BRNSS Publication Hub
The present study was aimed to investigate the phytochemical, antioxidant, and antibacterial studies of leaf and rachis of Dioon spinulosum Dyer ex Eichl. The phytochemical screening of the plant extracts revealed the presence of alkaloids, flavonoids, tannins, terpenoids, carbohydrate, and phenols, whereas saponin was absent. The phenolic content expressed as mg/g gallic acid equivalent was determined and was more in methanolic extract of leaf (29.40 mg) than rachis (8.76 mg). Flavonoid contents were also greater in leaves than in rachis and methanol extract contained higher content (2.812 mg/g) than water (1.923 mg/g). Terpenoids were more in the aqueous extracts of both leaf and rachis when compared to methanol extracts. Antioxidant activity of both leaf and rachis extracts was conducted using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power assay. Leaf extract showed more DPPH radical scavenging activity with IC50 value of 130 μg/ml when compared to the rachis of D. spinulosum Dyer ex Eichl. The reducing capability of the leaf extract was found to be more when compared to rachis. The antibacterial potential was evaluated with Staphylococcus aureus and Streptococcus mutans by agar well diffusion method. Antibacterial activity was observed only at higher concentration (1000 μg/ml) with inhibition zones of 12 mm and 13 mm.
Resorcinol is widely used in manufacturing of several drugs and pharmaceutical products that are mainly used
for topical ailments. The main objective of this study is to use an alternative strategy i.e., biofield treatment to alter
the physical, spectral and thermal properties of resorcinol.
Membrane Stabilizing And Antimicrobial Activities Of Caladium Bicolor And Che...IOSR Journals
The crude methanol extracts of whole plant of Caladium bicolor (Aiton) Vent. and leaf of Chenopodium album L. as well as their pet-ether, carbon tetrachloride, chloroform and aqueous soluble fractions were evaluated for membrane stabilizing and antimicrobial activities. At concentration 1.0 mg/ml, the carbon tetrachloride soluble fraction of C. bicolor inhibited 43.92±1.63% and 38.08±0.83 % hypotonic solution and heat induced haemolysis of RBCs, respectively. Among the extractives of C. album, the aqueous soluble fraction inhibited 47.11±0.49 % and 36.73±0.76 % hypotonic solution and heat induced haemolysis of RBCs as compared to 72.79 % and 42.12 % by acetyl salicylic acid (0.10 mg/ml), respectively. C. bicolor test samples demonstrated zone of inhibition ranging from 6.0 to 20.0 mm. The chloroform soluble fraction showed the highest zone of inhibition (20.0 mm) against Staphylococcus aureus. The test samples of C. album displayed zone of inhibition ranging from 7.0 to 13.0 mm. The highest zone of inhibition (13.0 mm) was showed by the chloroform soluble fraction against Salmonella paratyphi
Assessment of Antimicrobial, Anti-Inflammatory Activity and Docking Study of ...ijtsrd
A novel series of 2- arylimino -5- indole-2-yl-methylidene -1,3-thiazolidine-4-onederivatives were synthesized and screened for their anti-inflammatory and analgesic potential. The structures of newly synthesized compounds were confirmed by their analytical and spectroscopic data using IR and 1H-NMR. The novel compounds were evaluated for their anti-inflammatory potential using carrageenan induced paw edema model and analgesic activity using acetic acid induced abdominal writhing test. Three compounds 4g,4m and 4o alleviated inflammation more than the standard drug Diclofenac Sodium. The synthesized compounds also showed significant analgesic activity. In-silicomoleculardocking studies of the synthesized compounds were done on crystal structure of Cyclooxygenase-2using Glide version 5.0 following the standard procedure recommended by Schrodinger to study their observed activity, which revealed a significant correlation between the binding score and biological activity for these compounds. Maximum Glide score was obtained for compound 4o having a value of -7.42. This compound showed one interaction with the enzyme. Neetu Chopra | Kiranpreet Kaur | Sandeep Kaur "Assessment of Antimicrobial, Anti-Inflammatory Activity and Docking Study of Novel 2-(Arylimino)-5-(Indole-2-Yl-Methylidene)-1, 3-Thiazolidine-4-one Derivatives" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-6 , October 2018, URL: http://www.ijtsrd.com/papers/ijtsrd18822.pdf
Synthesis, Molecular Docking and Antimicrobial Evaluation of New Tetrahydrobe...ijtsrd
A series of novel derivatives of Tetrahydrobenzothienopyrimidine hydrazone were synthesized and product structure was elucidated by 1NMR, C13NMR and mass spectroscopy. The synthesized compounds were evaluated against fungal and bacterial strains. The synthesized compounds showed significant antibacterial activity against Staphylococcus aureus MTCC 96, Staphylococcus pyrogenes MTCC 442, and Escherichia coli MTCC 443, Pseudomonas aeruginosa MTCC 1688 and against fungal strains Candida albicans MTCC 227, Aspergillus niger MTCC 282, Aspergillus clavatus MTCC 1323. Some derivatives showed promising result against gram positive, gram negative bacterial and fungal strains than standard drug ampicillin and grieseofulvin. In- silico molecular docking studies of the synthesized compounds was done by using GRIP batch docking method of Vlife MDS 3.0 software to study their observed activity which showed a significant correlation between the binding score and biological activity for synthesized compounds. Neetu Chopra | Kiranpreet kaur | Sanjeev Kumar "Synthesis, Molecular Docking and Antimicrobial Evaluation of New Tetrahydrobenzothienopyrimidine Derivatives" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-6 , October 2018, URL: http://www.ijtsrd.com/papers/ijtsrd18756.pdf
International Journal of Engineering and Science Invention (IJESI)inventionjournals
International Journal of Engineering and Science Invention (IJESI) is an international journal intended for professionals and researchers in all fields of computer science and electronics. IJESI publishes research articles and reviews within the whole field Engineering Science and Technology, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Phytochemical Analysis of some Macrophytes of Lake Kondakarla, Visakhapatnam ...iosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Synthesis, Characterization and Biological evaluation of substituted Pyrazole...SriramNagarajan15
The work presented in this article consists of synthesis, characterization and biological evaluation of substituted pyrazole derivatives. Pyrazole derivatives have been shown to have wide variety of pharmacological activities like antimicrobial, antiviral, antihistaminic, antitumor, antipyretic, anti-inflammatory, antidepressant and anticonvulsant. As combination of biologically active moieties into one molecule and synthesis of totally newer moieties have been the methods of research, we present here synthesis of some novel pyrazole derivatives incorporating various biologically active aryl/aryloxy acid derivatives such as ibuprofen, diclofenac, aceclofenac as well as potent antibacterial quinolones, norfloxacin and ciprofloxacin. All the compounds synthesized were evaluated for their antimicrobial activity.
Bacterial pigments have many applications in current day to day life. The pigments produced by chromobacteria can be used for various applications like dairy, pharmaceutical, and food etc. In this study, three types of pigments were isolated i.e. yellow from Xanthomonas sp., pinkish Red from Rhodotorula sp., and orange from Sarcina sp. Pigmented bacterial isolates were obtained from the soil samples and used for the pigment extraction study. We studied that the pigment producing bacteria and identified the color producing pigments. Soil samples from Pondicherry, Cuddalore, Chennai, and Andhra sea coast were collected and used for isolation of microbes producing pigments. Purification of extracted pigments were done by column chromatography, whereas identification and characterization of purified pigment done by UV-Visible spectrophotometry and GC/MS analysis etc. The pigment isolated from bacterial sp. were used for the antimicrobial activity, antioxidant, and anticancer & transformation studies. The bacterial extracts of carotenoid pigment extracted and used as natural colorants for food products and dying of cloth.
Key-words: - Soil samples, GC/MS analysis, UV-Visible spectrophotometry, Carotenoid, Pigment extraction
Isolation of Alkaloid from a Medical Plant (A Case Study of Morinda Lucida)iosrjce
The isolation and detection of alkaloids content of Morinda lucida (Ezeogwu) from Rubiceace family,
a medicinal plant was carried out using solvent extraction process. The dried powdered leaves of the plant were
divided into batches. Different solvents were used on them. After 6 days of occasional shaking, it was filtered.
The filtrates were used for testing the presence of alkaloids in Morida Lucida. Mayer’s reagents Wagner and
Lugol’s reagents and 5m sodium hydroxide were used as detecting reagents. Mayer’s reagent yields cream
precipitate in both acidic and alkaline extracts. Wagner and Lugol’s reagents yield reddish brown precipitate in
both acidic and alkaline extracts. 5ml sodium hydroxide gave white swirling precipitate. Other coloured
precipitate like orange and pale orange was gotten as a result of difference in solvents used for isolation. The
presence of the above precipitate indicates the presence of alkaloids in Morinda Lucida.
Synthesis, Characterization and Biological Evaluation of Substituted Thiazoli...paperpublications3
Abstract: A new series of substituted thiazolidin-4-ones were synthesized and evaluated for antimicrobial activity by means of zone of inhibition by cup plate method. The structures of these compounds were established by means by IR, H NMR analysis. All the compounds were evaluated for their antibacterial activity against gram positive and gram negative species and antifungal activity .Compounds TH09 & TH17 were found most active due to presence of electron withdrawing groups at appropriate position.
The Role of Cell Wall-Degrading Enzymes in the Development of Anthracnose Dis...Agriculture Journal IJOEAR
— The ability of Colletotrichumtruncatum CP2 in producing pectinolytic and cellulolytic enzymes was evaluated by shake flask fermentations. The results of enzymatic activity experiment indicated that PG was the first cell wall-degrading enzymes detected and the activities obtained were higher (0.24±0.10 U/mL) than other enzymes, which appeared later and in lower amount. After the cell wall was degraded by the action of PG, further degradation of the cell wall was affected by pectin methylesterases, pectin lyase, pectate lyase and cellulases. The disparity in enzymatic activity at different intervals may suggest their specific role for pathogenesis at proper timings.
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
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Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
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Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
Basavarajeeyam is an important text for ayurvedic physician belonging to andhra pradehs. It is a popular compendium in various parts of our country as well as in andhra pradesh. The content of the text was presented in sanskrit and telugu language (Bilingual). One of the most famous book in ayurvedic pharmaceutics and therapeutics. This book contains 25 chapters called as prakaranas. Many rasaoushadis were explained, pioneer of dhatu druti, nadi pareeksha, mutra pareeksha etc. Belongs to the period of 15-16 century. New diseases like upadamsha, phiranga rogas are explained.
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New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
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Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...
B036105011
1. International Journal of Pharmaceutical Science Invention
ISSN (Online): 2319 – 6718, ISSN (Print): 2319 – 670X
www.ijpsi.org Volume 3 Issue 6 ‖ June 2014 ‖ PP.05-11
www.ijpsi.org 5 | Page
Synthesis,Characterization And In-Vitro Antimicrobial Studies Of
Cu(Ii)Complex With 1-[5-(1,3-Benzodioxol-5-Yl)-1-Oxo-2,4-
Pentadienyl]Piperidine Derived From Black Pepper
S.Radha1*
,S.Murugan1
,Dr.K.K.Mothilal2
,Dr.A.Thamarai selvan3
1
Department Of Chemistry,S.B.K.College,Aruppukottai,Tamilnadu.INDIA.
2
Department Of Chemistry,Saraswathi Narayanan College,Perungudi,Madurai,Tamilnadu,INDIA.
3
Research Department Of Chemistry,Thiyagarajar College,Madurai,Tamilnadu,INDIA.
ABSTRACT : Piperine is used in many different ways to influence our lives.The piperine in that spicy food has
a habit of stimulating perspiration, which in effect causes a cooling of the body. Thus very helpful in the south
where summer, temperatures can reach into the 100's.While piperine may sound like a pleasant spice, it can also
be a deadly killer. Piperine can be found in most insecticides, particularly those that killed the common
housefly. But perhaps piperine's most important use is in the medical field. For thousand of years people have
been using piperine to cure many small medical ailment.Instead of piperine,piperine complex has wide
biological application and also it act as more bioactive complex.This paper discussed the,Isolation of
(piperine) 1-[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]piperidine compound from pepper using suitable
solvent.Synthesis of Cu(II) complex with 1-[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]piperidine as a
ligand.Characterisation of prepared complex using UV, FT-IR, H1
-NMR Spectroscopy,Measurements molar
conductance of the above ligand and complex and investigation of anti-microbial activity of Cu(II) complex by
disc diffusion method.
KEY WORDS:Piperine,Spectroscopy,Molar conductance,Anti-microbial activity.
I. INTRODUCTION
Medicinal plants are important substance for the study of their traditional uses through the verification
of pharmacological effects and can be natural composite sources that act as new anti-infectious, anti-oxidant(
chien-chang et al.,1993).In order to find out new sources of drugs, a number of medicinal plants have been
screened for wide range of biological activities. About 3000 materials from 2,764 plant species have been
screened for their pharmacological and chemotherapeutic properties (Anon, 1988). Traditionally used
medicinal plants produce a veriety of compounds of known therapeutic properties(Lyengar,1976; Harborne,
1989; Chopra et al.,1992). Plants used in ethno medicine for the production of bioactive compounds are used
and rationalize the use of these medicinal plants in health care (Morales et al.,2008). Most of their properties are
due to secondary metabolites produced by medicinal plants. Transition metal complexes derived from herbal
medicines have been among the most widely studied coordination compounds in recent years (farrel et al.,
1984; Hondrellist et al.,1988;Bontchev et al.,2001), since they are becoming increasingly important as
biochemical (Apostolova et al., 1998), analytical (Golcu et al., 2001) and antimicrobial (Tumer,2000).Dried
ground pepper has been used since antiquity for both its flavor and as a medicine. Black pepper is the world's
most traded spice. It is one of the most common spices added to European cuisine and its descendants. The
spiciness of black pepper is due to the chemical piperine.This paper deals about synthesis,characterisation and
antimicrobial studies of Copper(II)complexes of 1-[5-( 1,3-Benzodioxol-5-yl)-1-oxo-2,4-pentadienyl ]
piperidine derived from black pepper.In piperine compound,active sites (N,O)donate electrons to copper metal
to form complex which was characterised by UV,FT-IR,NMR studies.
II. EXPERIMENTAL SECTIONS
SYNTHESIS OF of 1-[5-( 1,3-Benzodioxol-5-yl)-1-oxo-2,4-pentadienyl ] piperidine
EXTRACTION : About 10gm of pure ground pepper was taken in 100ml Round bottom flask and added 20ml
of Methylene dichloride with a magnetic stirrer. Attached a water condenser to the top of the flask and allowed
water to run through it to condense the methylene dichloride vapours. While refluxing the solution for 20mins.
After cooling the flask, used vaccum filtration with buchner funnel and filter paper to filtered out the pepper
grounds. Washed the grounds with 10ml Methylene dichloride and saved two drops of the filtrate for TLC
analysis.
2. Synthesis,Characterization and In-Vitro…
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ISOLATION : Transfered the filtrate to a 50ml RB flask and using a sand bath, removed the methylene
dichloride until a dark brown oil is left. Cooled the oil in an ice bath and added 6ml of cold ether. After stirring
for 5mins, removed the solvent again via sand bath heating. Cool the oil in an ice bath and added 6ml of cold
ether. Allow the flask to sit for 15mins in an ice bath with an occasional stirring. 1-[5-(1,3-benzodioxol-5-yl)-1-
oxo-2,4-pentadienyl]piperidine should precipitated out. Using the Buchner funnel, vaccum filter the yellow 1-
[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]piperidine crystal washed them with cold ether (2×4 ml).
III. PURIFICATION :
To crystallize, place the 1,3-benzodioxol-5-yl-1-oxo-2,4-pentadienyl in a test tube and dissolve it in
5ml of hot 3:2 acetone : hexane solution. stand for 15mins at room temperature and then 30mins in an ice bath,
vaccum filter the crystal using the Buchner funnel and washed with 4ml of cold ether. It may be necessary to
get a second crop of pale yellow crystals from the mother liquor to improve yield.
The structure of the 1-[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]piperidine are given below
Yield = 0.160gm
Melting Point = 1250
C
SYNTHESIS OF Cu(II) COMPLEX OF 1-[5-(1,3BENZODIOXOL-5-YL)-1-OXO-2,4-
PENTADIENYL]PIPERIDINE
About 0.285gm of 1-[5-(1,3-Benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]piperidine was taken in the
400ml beaker. Then it was dissolved with 15ml of methanol . About 0.121gm of the metal solution was added
to the beaker. It was stirred with magnetic stirrer for 4hours, which was covered with filter paper, After the
complete evaporation of methanol, the green crystal was appeared.
yield of the copper complex = 0.282gm
Melting point = 1460
C
FOURIER – TRANSFORMS INFRARED SPECTRASCOPIC STUDIES
Infrared spectroscopy gives information on molecular vibrations or more precisely on transitions
between vibrational and rotational energy level in molecules.
The SCHMADZHU Fourier Transform infrared spectrometer was used to record the FT-IR spectra 1-[5-(1,3-
benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]piperidine investigated the spectrum which was recorded in the range
from 400-4000 cm-1
.An Infrared spectrum is obtained when the frequency of molecule vibration correspond to
the frequency of the infrared radiation absorbed.
NUCLEAR MAGNETIC RESONANCE SPECTRASCOPIC STUDIES : The H1
-NMR spectra for the
samples were recorded in a Bruker 300MHZ NMR spectrometer using DMSO as the solvent. Two properties
of nuclear particles pertinent to an understanding of Nuclear Magnetic Resonance Spectrascopy are the net spin
associated with the protons and neutrons and the distribution of positive charge.
ULTRA-VIOLET SPECTROSCOPIC STUDIES :Ultra Violet absorption spectroscopy deals with the
measurement of energy absorbed when electrons are promoted to higher energy levels. The ultra-violet
spectrum is simply a plot of wavelength of light absorbed versus the absorption intensity absorbance or
transmittance and conveniently recorded by plotting molar asborptivity (E) against wavelength (nm) since (E)
values range in practice from a low as 10 to as high as 100000. It is convenient to use log ( ) as the abscissa of
UV spectrum.
ANTIMICROBIAL SCREENING PROCEDURE :The anti-microbial study of the ligand and their complex
were investigated using the procedure related to (Carr, et al 1972). 7gm of nutrient agar was measured and put
into 250ml of sterilized H2O. The mixture was heated for 15mins and placed in an autoclave at a temperature of
1210
C for about 24 hours. The prepared agar was poured into petri dish which was also sterilized. It was
3. Synthesis,Characterization and In-Vitro…
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allowed to sit in which holes were drilled by the use of sterilized cork borer. The organisms were rubbed on the
surface of the petri dish containing the nutrient agar. Various concentration of complex was introduced into the
hole with the use of the pipette . It was incubated for 24hours at a temperature of 370
C. A well cleared zone
around the nutrient agar indicates the antimicrobial effect of the complexes on the tested organisms.
IV. RESULT AND DISCUSSION
The functional group present in the molecule 1-[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-
pentadienyl]piperidine and its Copper (II)complex was identified with the help of FT-IR Spectrum and NMR
Spectrum and UV Spectrum.
FT-IR CHARACTERIZATION OF 1-[5-(1,3-BENZODIOXOL-5-YL)-1-OXO-2,4-
PENTADIENYL]PIPERIDINE
FUNCTIONAL GROUP ABSORPTION BANDS
(cm-1
)
C-H stretching vibration (Aromatic methylene dioxy
compounds) 2856
C=O Sretching vibration 1703
C=C stretching vibration 1634
C-N stretching vibration 1364
C-O stretching vibration (Aromatic methylene dioxy
compounds) 1252
Tri substituted benzene 1193
Table :1
The functional group present in 1-[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]piperidine was
identified with the help of the FT-IR spectrum.
FT-IR CHARACTERIZATION OF 1-[5-(1,3-BENZODIOXOL-5-YL)-1-OXO-2,4-
PENTADIENYL]PIPERIDINE Cu(II) COMPLEX
FUNCTIONAL GROUP ABSORPTION BANDS
(cm-1
)
C-H stretching vibration (Aromatic methylene dioxy
compounds) 2857
C=O Stretching vibration 1745
C=C stretching vibration 1631
C-N stretching vibration 1385
C-O stretching vibration (Aromatic methylene dioxy
compounds) 1251
Tri substituted benzene 1192
Table :2
FT-IR absorption bands (cm-1
) of the synthesized complex shown higher than their parent ligand . This
probably indicated the formation of complex UV Spectrum of 1-[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-
pentadienyl]piperidine Cu(II) complex From UV spectra it is known that the absorption of UV corresponding
to the copper complex was shown. The maximum absorption range is 342nm. This range is indicated copper
present in this complex. From this evidence we can elucidate the geometry of the complex may be tetrahedral.
4. Synthesis,Characterization and In-Vitro…
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NMR SPECTRUM OF 1-[5-(1,3-BENZODIOXOL-5-YL)-1-OXO-2,4-PENTADIENYL]PIPERIDINE
Cu (II) COMPLEX
From the H1
NMR spectral data for the complex exhibits all the expected signal C6H6 protons appeared at the
range 7.260ppm and heterocyclic ring appeared at the range 1.35ppm, aliphatic protons appeared at the range
4.854ppm and 3.35ppm, respectively.
MOLAR CONDUCTIVITY MEASUREMENT: Small quantity(0.01mol) of ligand / complex was taken in
the 100ml beaker then it was dissolved in 10ml of DMSO. The conductivity cell was dipped into the solution
and the conductance was measured by using systronics conductivity meter.Minimum Conductance(0.07mho cm2
mol-1
)showed that the complex might be non- electrolyte.
5. Synthesis,Characterization and In-Vitro…
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ANTI-MICROBIAL STUDIES OF 1-[5-(1,3-BENZODIOXOL-5-YL)-1-OXO-2,4-
PENTADIENYL]PIPERIDINE AND ITS COMPLEX
Ligand/
Complex
ZONE OF INHIBITION (mm)
BACTERIA FUNGI
Bacillusceres Stephylococcusaureus
Candida
Albicans
SML R R 12
SMCU 10 12 16
Table :3
Standard disc – Bacteria (Amikacin) Fungi (Ketokonazole)
SML – Ligand , SMCU – Metal complex , R – Resistant.
BACTERIAL STUDIES
STAPHYLOCOCCUS AUREUS
BACILLUS CEREUS
FUNGICIDALACTIVITY
CANDIDAALBICANS
6. Synthesis,Characterization and In-Vitro…
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From table 3,We can infer that the complex would be more biologically active than the Parent ligand.
V. CONCLUSION
1-[5-(1,3-Benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]piperidine ligand was isolated from black pepper
then it was binded with Cu(II) metal by using conventional method. The functional groups in the ligand and
complex were confirmed by the Fourier – Transforms infrared spectroscopy (FT-IR), Nuclear Magnetic
Resonance spectroscopy (NMR) , Ultra – Violet spectroscopy (UV) .The Infrared spectrum of both ligand and
complex were presented in table1&2 in which comparisons were made between the ligand and complexes.The
melting point of the synthesized complex shown higher than their parent ligand. This is probably indicates the
formation of complex.The result obtained from conductivity measurement showed that the complex is non
electrolyte.The antimicrobial activities were evaluated on both the ligand and the complexe by determining
the measurements of the zone of inhibition using Bacilluscereus and Stephylococcusaureus, Candida Albicans
which was indicated in table 3. From the result obtained, comparisons were made between the ligand and the
complex, the complex had higher antimicrobial activities than their parent ligand.
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