1) The study determined the total phenolic, flavonoid, and tannin content in chloroform, ethyl acetate, ethanol, and aqueous extracts of Pyrus communis fruit.
2) The results showed that the ethyl acetate and ethanol extracts contained significantly higher levels of phenolics, flavonoids, and tannins compared to the aqueous and chloroform extracts.
3) Specifically, the ethyl acetate extract had the highest concentration of total phenolics, while the ethyl acetate and ethanol extracts had similar high levels of total flavonoids.
การวิเคราะห์ฤทธิ์ต้านอนุมูลอิสระที่ระยะต่างกันในกล้วยเล็บมือนาง
Analysis of Antioxidant Activity at Different Stage in Musa (AA group) ‘Kluai Leb Mu Nang’
อดิศร จำรูญ
Herbal remedies are plants used as medicine.
People use herbal remedies to help prevent or cure disease.
They use them to get relief from symptoms, boost energy, relax, or lose weight.
Herbal remedies are a type of dietary supplement. They are not medicines.
Herbals are not regulated like medicines.
Herbals do not need to be rigidly tested before they are sold.
Herbals may not work as claimed.
Labels do not need to be approved.
It may not list the correct amount of an ingredient.
Drug discovery is the process through which potential new medicines are identified.
It involves a wide range of scientific disciplines, including biology, chemistry and pharmacology.
Natural products and their structural analogues have historically made a major contribution to pharmacotherapy, especially for cancer and infectious diseases.
Natural products also have challenges for drug discovery, such as technical barriers to screening, isolation, characterization and optimization.
INDUSTRIAL PHARMACOGNOSTICAL TECHNOLOGY
Herbal drug industry: Infrastructure of herbal drug industry
involved in production of standardized extracts and various
dosage forms. Current challenges in upgrading and
modernization of herbal formulations. Entrepreneurship
Development, Project selection, project report, technical
knowledge, Capital venture, plant design, layout and construction.
Pilot plant scale –up techniques, case studies of herbal extracts.
Formulation and production management of herbals.
In Ayurveda, the leaf juice of Adhatoda vasica, a shrub native to Asia is incorporated in
many traditional herbal formulations. However, suitable solvent and a suitable extraction
method for phytochemical profiling are not well established, and there is no published mass
spectra structural interpretation of the identified compounds. This has caused a few
problems in herbal formulation research due to the bias derived from different extraction
methods. Therefore, this study used polar and non polar extraction for phytochemical
analysis on Adhatoda vasica, aiming to assess the potential impact of different solvents. This
study included extractive value, total phenol and alkaloid content of the leaves in different
preparations. Gas Chromatography coupled with Mass Spectrometry (GC-MS) was used to
study the phytochemical profile of different solvents. Significant differences were observed in
all the parameters such as extract yield, total phenol, total alkaloid and phytochemical
composition. The ethanol extract stood out most for effective extraction of phytochemicals,
especially for the alkaloids. The results highlight the necessity for comparative analyses of
chemical composition in different solvent extractions and careful choice and validation of
analytical methodology in herbal formulation research.
การวิเคราะห์ฤทธิ์ต้านอนุมูลอิสระที่ระยะต่างกันในกล้วยเล็บมือนาง
Analysis of Antioxidant Activity at Different Stage in Musa (AA group) ‘Kluai Leb Mu Nang’
อดิศร จำรูญ
Herbal remedies are plants used as medicine.
People use herbal remedies to help prevent or cure disease.
They use them to get relief from symptoms, boost energy, relax, or lose weight.
Herbal remedies are a type of dietary supplement. They are not medicines.
Herbals are not regulated like medicines.
Herbals do not need to be rigidly tested before they are sold.
Herbals may not work as claimed.
Labels do not need to be approved.
It may not list the correct amount of an ingredient.
Drug discovery is the process through which potential new medicines are identified.
It involves a wide range of scientific disciplines, including biology, chemistry and pharmacology.
Natural products and their structural analogues have historically made a major contribution to pharmacotherapy, especially for cancer and infectious diseases.
Natural products also have challenges for drug discovery, such as technical barriers to screening, isolation, characterization and optimization.
INDUSTRIAL PHARMACOGNOSTICAL TECHNOLOGY
Herbal drug industry: Infrastructure of herbal drug industry
involved in production of standardized extracts and various
dosage forms. Current challenges in upgrading and
modernization of herbal formulations. Entrepreneurship
Development, Project selection, project report, technical
knowledge, Capital venture, plant design, layout and construction.
Pilot plant scale –up techniques, case studies of herbal extracts.
Formulation and production management of herbals.
In Ayurveda, the leaf juice of Adhatoda vasica, a shrub native to Asia is incorporated in
many traditional herbal formulations. However, suitable solvent and a suitable extraction
method for phytochemical profiling are not well established, and there is no published mass
spectra structural interpretation of the identified compounds. This has caused a few
problems in herbal formulation research due to the bias derived from different extraction
methods. Therefore, this study used polar and non polar extraction for phytochemical
analysis on Adhatoda vasica, aiming to assess the potential impact of different solvents. This
study included extractive value, total phenol and alkaloid content of the leaves in different
preparations. Gas Chromatography coupled with Mass Spectrometry (GC-MS) was used to
study the phytochemical profile of different solvents. Significant differences were observed in
all the parameters such as extract yield, total phenol, total alkaloid and phytochemical
composition. The ethanol extract stood out most for effective extraction of phytochemicals,
especially for the alkaloids. The results highlight the necessity for comparative analyses of
chemical composition in different solvent extractions and careful choice and validation of
analytical methodology in herbal formulation research.
Phytochemical Analysis of some Macrophytes of Lake Kondakarla, Visakhapatnam ...iosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Total Phenol and Antioxidant from Seed and Peel of Ripe and Unripe of Indones...iosrphr_editor
Study on total phenol and antioxidantactivity ofsugar apple fruits of various solvent, part of fruits, and level of ripening. Solvent extraction used were 80% (v/v) methanol, 50% (v/v) acetone, boiling water, and 50% (v/v) ethanol. Part of fruits thatbeen used for samples were seed and peel which are normally by products of sugar apple processing, level of ripening were unripe, and ripe sugar apple fruits. Total phenol was determined by Folin-ciocalteau method. Total antioxidant was quantified by 1,1-diphenyl-2-picrylhydrazyl(DPPH) method.Therewas a difference in type of solvent, part of fruits, and level of ripeningon total phenol and antioxidant concentration of sugar apple fruits. Seeds have higher total phenol concentration than peels of this fruits. Unripe sugar apple fruits have higher total phenol and antioxidant than ripe fruit. The best solvent for phenol extraction was ethanol 50%butthe best solvent for antioxidant extraction was acetone 50%.
The word “banana” is a general term comprising a number of species or hybrids in the genus Musa of family Musaceae. Many studies reported that banana is a good source of phenolic compounds, which are having potential health benefits in human beings. The phytochemical composition of eight banana varieties was investigated. Quantitative estimation of phytochemicals revealed that highest alkaloid and tannin content was noticed in variety Nendran (3.76% and 4.40 mg/100g respectively). Whereas, variety Rasakadali exhibited highest flavonoid content (9.49 mg/100). Total phenol and saponin content was found higher in variety Kadali (0.82% and 11.6 mg/100g respectively).
Screening of antioxidant phytoextracts of Canarium odontophyllum (Miq.) leave...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
Phytochemical Screening, Antioxidant, and Antibacterial Activity of Dioon spi...BRNSS Publication Hub
The present study was aimed to investigate the phytochemical, antioxidant, and antibacterial studies of leaf and rachis of Dioon spinulosum Dyer ex Eichl. The phytochemical screening of the plant extracts revealed the presence of alkaloids, flavonoids, tannins, terpenoids, carbohydrate, and phenols, whereas saponin was absent. The phenolic content expressed as mg/g gallic acid equivalent was determined and was more in methanolic extract of leaf (29.40 mg) than rachis (8.76 mg). Flavonoid contents were also greater in leaves than in rachis and methanol extract contained higher content (2.812 mg/g) than water (1.923 mg/g). Terpenoids were more in the aqueous extracts of both leaf and rachis when compared to methanol extracts. Antioxidant activity of both leaf and rachis extracts was conducted using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power assay. Leaf extract showed more DPPH radical scavenging activity with IC50 value of 130 μg/ml when compared to the rachis of D. spinulosum Dyer ex Eichl. The reducing capability of the leaf extract was found to be more when compared to rachis. The antibacterial potential was evaluated with Staphylococcus aureus and Streptococcus mutans by agar well diffusion method. Antibacterial activity was observed only at higher concentration (1000 μg/ml) with inhibition zones of 12 mm and 13 mm.
Pharmacognostic, antioxidant and antimicrobial activity of fractions of the l...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
Phytochemical and antioxidant studies of methanol and chloroform extract from...shailendradhakal
This research was carried out with the aim of phytochemical analysis and determining antioxidant activity present in methanol and chloroform leaf extracts of Azadirachta indica. Due to its potential in curing various ailments as well as wide spread application of antioxidant activity such as in the field of cosmetology, the plant was selected for the study. The total phenolics contained in the plant extracts were also studied which are responsible for the antioxidant activity.
Patient compliance: Challenges in management of cardiac diseases in Kuala Lum...pharmaindexing
Background
The objective of this study was to investigate the degree of compliance among cardiac patients who attend the health facilities in Kuala Lumpur and Perak, Malaysia. The reasons for non-compliance and recommendations from healthcare professionals were also evaluated.
Method
A cross-sectional study of 400 patients and 100 healthcare professionals was carried out. This study utilizes variables on external factors and internal factors as the measurement tools. The questionnaire which consists of Morisky self-reported medication adherence questions was administered to patients and causes for non-compliance sought. Questionnaire for healthcare professionals was used to determine strategies that can improve compliance rate.
Results
The study revealed a 15.8% of high adherence rate, 54.3% of moderate adherence rate and 30% of poor adherence to cardiovascular disease medications. The chi-square tests showed the strong association between dependent and independent variables. The model chosen for testing the patient compliance through external and internal factors gives an R2 value of 85.0% with an adjusted R2 of 84.7%. The F value (317.187) was also significant (p=0.000) which means that the variables have better fit in the multivariate model. The major reasons determined for non-adherence were attitudes and beliefs, lifestyle, side effects and cost of medications. The study recommends that pharmacists and dispensing technicians should be adequately qualified to provide proper counselling to cardiac patients on their medicines and disease conditions.
Conclusion
The result of this study is of value to health care providers. Compliance to cardiovascular medications will avoid treatment failures encountered in therapy.
Overview on Recurrence Pregnancy Loss etiology and risk factorspharmaindexing
Recurrent pregnancy loss (RPL) can be defined as more than two to three consecutive miscarriages before 20 weeks’ gestation; it affects approximately 1% to 2% of women. RPL is a multifactorial disease. It is very important to study the etiology and risk factors of RPL to find the best diagnostic tests and suitable therapeutic intervention. This article will discuss the current understanding etiologies and risk factors of RPL.
Novel treatments for asthma: Corticosteroids and other anti-inflammatory agents.pharmaindexing
Asthma management is a challenge due to the prevalence of disease in the world. Based on the immunological and inflammatory mechanisms of asthma, corticosteroids and anti-inflammatory participate greatly in the treatment plan. Due to different reasons, there is still an unmet need to develop new agents in this field. A lot of compounds with anti-inflammatory effect are investigated in both pre-clinical and clinical studies.
A review on liver disorders and screening models of hepatoprotective agentspharmaindexing
The liver is a vital organ present in vertebrates and some other animals. It has a wide range of functions, including detoxification, protein synthesis, and production of bio chemicals necessary for digestion. The liver is necessary for survival; there is currently no way to compensate for the absence of liver function long term, although liver dialysis can be used short term.
Carbamazepine induced Steven Johnson syndrome: A case reportpharmaindexing
Drugs are the most common cause that induces Steven Johnson syndrome (SJS) and includes antiepileptic drugs, antiretroviral drugs, anti-tuberculosis drugs, Sulphonamides, fluoroquinolones, penicillins, non-Steroidal anti-inflammatory drugs, Multivitamins. The genetic markers are also the cause for carbamazepine induced Steven Johnson Syndrome. In our study, the antiepileptic drug (Carbamazepine) is the cause for Steven Johnson Syndrome. A female patient aged 25 years came to the hospital with the complaints of bubbling over the skin and all over the body with papillary vesicles associated with pain and irritation, fever, myalgia, and nausea. The patient is known case of Phenytoin induced Steven Johnson Syndrome. In this case the patient developed the Steven Johnson Syndrome approximately after one month after starting the carbamazepine.By the withdrawal of the drug, the condition of the patient was improved.
Monoherbal formulation development for laxative activitypharmaindexing
The Ayurvedic Pharmacopoeia specifically approves flaxseed as a poultice for boils externally and demulcent or laxative internally. In this study monoherbal formulation development for laxative activity of flaxseed was undertaken. The plantLinumusitatissimumhasshowed higher percentage of total ash as well as alcohol soluble extractive values. The aqueous extract of Linumusitatissimumwas prepared by using pilot scale extraction plant and spray drying unit. The qualitative phytochemical studies reveal the presence of amino acids, carbohydrates, vitamins and proteins. From the available literatures it was found that Linumusitatissimum contains more number of amino acids. The formulated tablets showed acceptable pharmacopoeial limits and complies with specifications for thickness, hardness, friability and weight variation. The formulation has showed better laxative activity indicating additive property of the combined phytoconstituents of the plant.
Monoherbal formulation development for laxative activitypharmaindexing
The Ayurvedic Pharmacopoeia specifically approves flaxseed as a poultice for boils externally and demulcent or laxative internally. In this study monoherbal formulation development for laxative activity of flaxseed was undertaken. The plantLinumusitatissimumhasshowed higher percentage of total ash as well as alcohol soluble extractive values. The aqueous extract of Linumusitatissimumwas prepared by using pilot scale extraction plant and spray drying unit. The qualitative phytochemical studies reveal the presence of amino acids, carbohydrates, vitamins and proteins. From the available literatures it was found that Linumusitatissimum contains more number of amino acids. The formulated tablets showed acceptable pharmacopoeial limits and complies with specifications for thickness, hardness, friability and weight variation. The formulation has showed better laxative activity indicating additive property of the combined phytoconstituents of the plant.
Pneumonia and respiratory failure from swine origin influenza H1n1pharmaindexing
Swine influenza (swine flu) became alarming health concern when World Health Organization declared as “public health emergency of international concern” on April 25, 2009. After documentation of human-to-human transmission of the virus in at least three countries of two WHO regions, the WHO raised the pandemic level to 6.1 During the 1918, flu pandemic infected one-third of the world's population (an estimated 500 million people) and caused approximately 50 million deaths.2 In 1976, an outbreak of swine influenza occurred in New Jersey, USA, which involved more than 200 cases, some of them severe, resulting in one death.3 In 1988, another fatality was reported as a complication of swine influenza.
A descriptive study on newborn care among postnatal mothers in selected mater...pharmaindexing
The newborn health challenge faced by India is more formidable than that experienced by any other country in the world. The newborn health is inevitably affected by the traditional care practices of the mothers causing high infant morbidity and mortality.The aim of the study were determine the knowledge, attitude and practice of postnatal mothers regarding new born care and find out the association between knowledge, attitude and practice of postnatal mothers regarding new born care and to determine the association between these as well as with the selected demographic variables. A descriptive study was conducted to assess the knowledge, attitude and practice of postnatal mothers regarding new born care in selected maternity centres in Madurai. Survey approach was employed to select sample and it consisted of 100 postnatal mothers. Data was collected using structured interview schedule. Findings of the study showed that 65% of postnatal mothers had moderate knowledge; 61% had favourable attitude and 57% of them had high practice of new born care. There was a significant association between knowledge and attitude (r=+0.567), knowledge and practice (r=+0.388), attitude and practice (r=+0.321) .There was a significant association between knowledge and education, monthly family income and obstetrical score at p<0.05. Findings of the study indicated the need to conduct frequent assessment of knowledge, attitude and practice of postnatal mothers regarding new born care. Awareness and attitude of the mothers towards new born care still has lots of lacunae especially in those who belong to the lower socio economic statusand poorly educated postnatal mothers. So it is imperative to provide comprehensive training in the field of new born care for mothers during pregnancy
Late 19th century was evident of intelligent biomaterial; which has changed researcher’s perspective towards science and technology. This intelligent biomaterial are envisioned to have huge impact on Healthcare from sequential signalling of biomedical molecule, mimicking natural gene, an effective drug carrier, to high resolution diagnostic tool.From drug discovery aspect many of NCE fail to reach therapeutic potential due to PK/ PD profile. Nanotechnology has changed the face of drug discovery form chemical evaluation to structure of proteins in signalling pathways and development of chemical antibody. Nanotechnology from lab to market approval is long process due to regulatory evaluation. Though it seems to be bright future market it has to go through a long process from being innovation to complete market product. This makes whole process expensive making investor reluctant to invest in big projects.Western world is aware of dramatic potential of nano-projects; which has its limitation in financial investments; with major challenge of transforming nano science to commercial pharmaceutical product.
The Flaws in health practice in post-operative management of a patient in ter...pharmaindexing
Introduction
Congenital urinary tract obstructions are common cause of kidney damage sometimes which sometimes presents itself without symptoms leading to abnormalities in blood filtration and consequently retarded kidney function. A cohort study was conducted in such patient to find out the short comings in treatment strategy.
Case presentation
A four years old child, weighing 14 kg was brought with severe constipation, fever, chest congestion and cough later developed left eye disorientation after admission to hospital, diagnosed with urinary tract obstruction, indicating acidosis and loss of electrolytes due to excessive loss of water. His therapy management included surgical treatment, dialysis and to improve his electrolyte levels within the normal with the treatment chest congestion and fever.
Conclusion
This case study reports the post operative treatment of congenital urinary tract obstructions in a tertiary care hospital and highlights the discrepancies observed. Antibiotic rationality and irrational prescribing was observed. The case study highlights the need of a clinical pharmacist in the health care team.
Corticosteroid induced disorders – An overviewpharmaindexing
Glucocorticoids are important in the treatment of many inflammatory, allergic, immunologic, and malignant disorders, and the toxicity of glucocorticoids is one of the commonest causes of iatrogenic illness associated with chronic inflammatory disease.Glucocorticoid-induced muscle atrophy is characterized by fast-twitch or type II muscle fiber atrophy. Corticosteroid (CS) therapy is widely used in the treatment of rheumatic diseases.Osteoporosis remains one of its major complications.Steroid induced glaucoma is a form of open angle glaucoma occurring as an adverse effect of corticosteroid therapy. Glucocorticoids induce hepatic and extrahepatic insulin resistance.Glucocorticoid treatment impairs both glucose transport in fat and muscle cells. Corticosteroid-induced psychosis represents a spectrum of psychological changes that can occur at any time during treatment. Cushing’s syndrome describes the signs and symptoms associated with prolonged exposure to inappropriately high levels of the hormone cortisol. Physicians must be aware of these adverse effects and be equipped to manage them.
Anti-inflammatory activity of pupalia lappacea L. Jusspharmaindexing
Pupalia lappacea (L) Juss is an erect shrub used in folklore medicine to treat bone fractures and in inflammatory conditions. Methanolic extract of aerial parts shown is claimed in traditional medicine that the leaves of the plant are used in the treatment of inflammation. In the present study, the methanolic extract of Pupalia lappacea was screened for its anti-inflammatory activity using carageenan induced rat paw edema egg white induced paw oedema models. The methanolic extract at the dose of 200 mg/kg p.o exhibited significant anti-inflammatory activity in carrageenan induced paw edema model (p<0.01). In egg white induced model, methanolic extract at the dose of 200 mg/kg inhibited paw oedema significantly (p<0.01) indicating that both test samples inhibit the increase in number of fibroblasts and synthesis of collagen and mucopolysaccharides during prostaglandin formation during the inflammation. These experimental results have established a pharmacological evidence for the folklore claim of the drug to be used as an anti inflammatory agent. HPTLC analysis of the extract shows the presence of gallic acid 1.24mg/ml, ferulic acid 2.00mg/ml, chlorogenic acid 46.25mg/ml and rutin 7.02mg/ml of the extract which were responsible for the claimed anti-inflammatory action in the animal models studied.
Lucinactant: A new solution in treating neonatal respiratory distress syndrom...pharmaindexing
Lucinactant is a novel synthetic surfactant, approved by the FDA on March 6th 2012, for use in treatment of RDS. It’s superiority as compared to the previously approved surfactants lie in containing sinapultide, a 21-amino acid peptide also known as KL4 peptide, which has been designed to mimic the activity of human surfactant protein. Lucinactant is completely devoid of any animal derived components. It is the fifth drug approved by the FDA for the treatment of RDS. It has shown immense efficacy in phase two clinical trials and animal model studies and exhibited better efficiency when compared to other surfactants in both 24 hour and two week mortality rates of infants in RDS. Lucinactant tends reduce the surface tension at the air-liquid interface of alveolar surfaces and allows lungs to function normally. It was observed that the side effects were lesser with Lucinactant when compared with other naturally derived surfactants.
Bioactivity screening of Soil bacteria against human pathogenspharmaindexing
Microorganisms have a profound effect on medical science as they not only infect & cause disease but also produce metabolic products that can cure infections. Soil happens to be a source for a variety of microorganisms. Most of the bacteria, particularly actinomycetes produce biologically active secondary metabolites. Though there are a number of antibiotics available, there is a pressing need for the discovery of new source for antimicrobials against the pathogens due to the development of drug resistance of the pathogenic microorganisms. In addition to, new pathogenic strains are also developing and causing infection to human beings. Bioactive compounds are compounds that are produced by any living organism and are known to exhibit various biological activities both in-vitro & in-vivo. Bioactivity may be antimicrobial, antineoplastic, anticancerous, immunomodulation, antifertility & others. Soil bacteria were isolated by standard technique and by making use of selective media. The isolates were identified and subjected for preliminary screening to look for their ability to produce bioactive materials. A total of 96 strains were isolated from three different soil samples. 14 of them were found to have antibacterial activity against the human pathogens like Staphylococcus aureus, Streptococcus faecalis, E.coli, Klebsiella aerogenes, Proteus vulgaris, Pseudomonas aureginosa and Salmonella typhi by preliminary screening. Further the selected (3) bacteria were grown in the suitable culture media for the production of bioactive metabolites by using rotary shake flask. The active metabolites was isolated by solvent extraction and concentrated by evaporation under reduced pressure. The antimicrobial screening of the active metabolites showed prominent effect against the clinical pathogens under the study.
A study on sigmoid Volvulus presentation and managementpharmaindexing
A study on sigmoid volvulus presentation and management was a 2yr retrospective study done at RMMCH.The diagnosis of sigmoid volvulus was made from a history of large bowel obstruction (constipation, abdominal distension, and abdominal pain), which were often recurrent and plain abdominal radiographs.The morbidity associated isSuperficial wound infection occurred in four patients. All the infected wounds eventually healed with conservative measures. Clinical anastomotic dehiscence was noted in 1 patient for which during relaparotomy proximal colostomy and mucous fistula was done. The mortality associated is shown is there were 9 deaths of which 7 were due to sepsis and 2 were due to comorbid illness. Two out of eight patients for whom a colopexy was done had a recurrent attack of sigmoid volvulus. The duration of hospital stay ranged between 10 and 21 days. Use of sigmoidoscopic detorsion for viable colon should be encouraged. Sigmoidopexy, which is associated with a recurrence rate of 20% in our series of patients, should be used selectively.Hartmann’s procedure is a safe option in sigmoid volvulus with gangrenous bowel. Primary anastomosis in emergency situation can be carried out with morbidity and mortality in patients with viable colon
Evaluation of Preliminary phytochemical on various some medicinal plantspharmaindexing
The present study was carried out to evaluate the physical status and percentage yield of methanolic extract and its fractions of whole plant of Leucas cephalotes, leaves of Hiptage benghalensis and leaves of Kydia calycina were recorded for future references and Preliminary phytochemical screening of MLC, MHB and MKC revealed the presence of carbohydrates, glycosides, saponins, flavonoids, steroidal and phenolic compounds. MLC revealed the presence of all the above mentioned phytoconstituents except saponins and also MKC steroidal compounds. The fractions of MLC, MHB and MKC revealed the presence of glycosides, phenolic compounds, steroids and flavonoids.
Comparision of in vitro antibacterial activity of cefoperazone and levofloxac...pharmaindexing
Cefoperazone (a third generation cephalosporin) has effective in vitro activity against majority of pathogens. Levofloxacin (a flouroquinolone) is one which prescribed more due to its increased antibacterial activity against Gram-positive, Gram-negative, and atypical bacteria. Microbial resistance to antibiotics is now prevalent and poses a serious clinical threat. An attempt has been made to evaluate sensitivity of Cefoperazone and Levofloxacin against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella typhi. A total of 120 isolates were collected from different pathological laboratories and medical centers in Karachi, Pakistan. The above stated clinical isolates were extracted from urine/stool, skin, blood and sputum samples. Results show least resistance of Levofloxacin as compare to Cefoperazone against Escherichia coli (32.5% and 42.5%) and Pseudomonas aeruginosa (36% and 48%) while Staphylococcus aureus is still susceptible towards Cefoperazone and least sensitive to Levofloxacin by showing 26.6% and 50% resistance respectively. Study concluded that the prevalent pathogens are still susceptible towards Levofloxacin and Cefoperazone but the gradual increase in resistance is alarming to the general practice of prescribing antibiotic which require routine evaluation and surveillance to ensure the effectiveness of the antibacterial agents.
Concept of srotas from ayurvedic perspective with special reference to neurologypharmaindexing
Ayurveda is a life science. The researchers of ayurveda could rule out the presence of srotas (channels) spreading throughout the human body. These srotas (channels) are governed by vayu which is using all the srotas (channels) of the body to carry out the functional and physiological activities of the human body without which the human society will not exist. Several synonymous words have been described by the ayurvedicacharyas for srotas. Some are micro and some are macro in structures and they adopt the same colour of the particular dhatus of the body to which it belongs. The aim of the study is to justify that srotas are nothing but innurmerable channels or pathways of the nervous system governed by electric current without which no functional and physiological activities of the human body will develope.
Health promotion survey in overweight and obese students of universities in n...pharmaindexing
Introduction
Overweight and obesity is one of the major health problems in the UK and worldwide. Approximately two-thirds of the population in the UK is either overweight or obese. Overweight and obesity is an important issue that causes distress to most women. Health promotion is the best method to educate overweight and obese women. It is defined as the process enabling people to increase control over and to improve their health by Ottawa Charter for Health Promotion. It is aimed to enhance the well-being of the individuals and their positive attitudes towards prevention of various diseases. In order to make any improvement to the health promotion for overweight and obesity, the risk factors and the opinions from the public should first be identified and addressed.
Methods
Cross-sectional survey design was selected with a questionnaire that consisted of 20 open and close ended questions. A sample size of 196 was determined. The data thus gathered was analyzed using SPSS V20 (Statistical Package for Social Science version 20). Descriptive statistics (fx) and (SD) were used and Chi-square X2 test for association was employed.
Results
Out of the total 196 responses, only (40%) of the students had normal weight (SD 1.1), (25%) students had a good understanding of health promotion (SD 1.6), half (50%) appeared concerned about their weight (SD 0.5), (60%) had an obese family member (0.5). The BMI of students was associated with the presence of an obese member in their family and their weight as a concern for them. (P-value <0.05).
Conclusion
The health promotion service is beneficial as it was found to have raised concerns in the mind of the students regarding over weight and obesity. However it was observed that the understanding of health promotion service was different among students and this is the root of the problem.
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
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Total phenolic, flavonoids and tannin content of various extracts from Pyrus communis fruit
1. Velmurugan Chinnasamy, et al / Int. J. of Pharmacy and Analytical Research Vol-3(4) 2014 [384-390]
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IJPAR |Vol.3 | Issue 4 | Oct-Dec-2014
Journal Home page: www.ijpar.com
Research article Open Access
Total phenolic, flavonoids and tannin content of various extracts from
Pyrus communis fruit
Velmurugan C*1
and Anurag bhargava2
1
Research scholar, Institute of Pharmaceutical sciences and Research center, Bhagwant University, Sikar
road, Ajmer, Rajasthan, India-305004.
2
Department of Pharmacognosy, CH. Devi lal College of Pharmacy, Bhagwangarh, jagadhri, Haryana,
India-135003.
* Corresponding author: Velmurugan Chinnasamy
E-mail id: velu0906@gmail.com.
ABSTRACT
The pyrus communis commonly known as Pear fruit having numerous pharmacological properties. Natural bioactive
compounds like phenols, tannin and flavonoids are the important secondary metabolites in plant possess wide range
biological action and this will supported with scientific studies on these metabolite from pear fruit. To maximize these agents
in the extract different solvents viz. chloroform, ethyl acetate, ethanol and aqueous are used for the extraction procedure.
Current study was aimed to determine the levels of total phenolic, flavonoids and tannin contents. Observations suggested
that ethyl acetate and ethanol extracts has significantly high (P<0.001) concentration of flavonoids, phenolic and tannin
contents as compared to aqueous and chloroform extracts. Therefore, ethyl acetate and ethanol extracts of pyrus communis
has greater potential to produce more beneficial effects in biological system as compared to aqueous and chloroform extracts.
Keywords: Pyrus communis, Flavanoid, tannin and phenolic compound
INTRODUCTION
The Pear (Pyrus communis L.) is among the most
economically important fruit tree crops of the
temperate zones [1]
. It belongs to family Rosaceae. It
also called Common Pear- in English, in Hindi –
Babbugoshaa, in Sanskrit – Amritphala and tamil-
perikkai. Ancient Greek poet Homer described Pears as
one of the ‘gifts of God’. This prehistoric fruit has been
under cultivation both in Europe and Asia for long
times, also known as European Pear [2] Sand pear
(Japanese and Chinese species) has been domesticated
as edible fruit and cultivated in Asia for more than
3000 years [3].It has astringent, sedative activity, act as
febrifuge. Its leaves and bark can be used in wound
healing on account of their astringent action. [4]
It is an
antioxidant. It acts against reactive Oxygen species [5,
6]
. The flowers of common pear are used in folk
ISSN: 2320-2831
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medicine as components of analgesic and spasmolytic
drugs [7]
. Natural bioactive compounds like phenols and
flavonoids are the important secondary metabolites in
plants having intrinsic properties that affect
appearance, taste, odor and oxidative stability of plant
based foods. These compounds also possess biological
properties like antioxidant, anti-aging, anti-carcinogen,
protection from cardiovascular, immune/autoimmune
diseases and brain dysfunctions viz. Parkinson’s,
Alzheimer’s, Huntington’s diseases, etc [8, 9]
. Due to
their large biological activities, plant secondary
metabolites have been used for centuries in traditional
medicine. Nowadays, they correspond to valuable
compounds such as pharmaceutics, cosmetics, fine
chemicals, or more recently nutraceutics. Recent
surveys have established that in western countries,
where chemistry is the backbone of the pharmaceutical
industry, 25% of the molecules used are of natural
plant origin [10]
.
Therapeutic potential of Pyrus communis extract is
directly related to total phenolic, Tannin and
flavonoids contents. These active metabolites
especially from herbs are the interest subject of
research, but their extraction as part of phytochemical
or biological investigations presents specific challenges
that must be addressed through out the solvent
extraction process. Therefore, present study was aimed
to investigate the levels of phenolic, flavonoids and
tannin contents in different extracts prepared using
chloroform, Ethyl acetate, ethanolic and aqueous
solvents by spectrophotometric methods.
MATERIAL AND METHODS
Chemicals
Chloroform, ethanol, petroleum ether, ethyl acetate and
sodium bi carbonate were purchased from BVN
Chemicals, India. Standards of phenolic acids (gallic
acid), tannin (tannic acid) and of flavonoids (rutin
hydrate) were purchased from loba chemie Pvt Ltd,
Mumbai. The Folin- Ciocalteu’s phenol reagent and
Folin denis reagentwere from s d fine chem ltd.
Aluminium chloride (AlCl3) were from Indian
chemicals, Bangalore, India. All other solvents and
chemicals were of analytical grade.
Collection and authentication of the plant
material
The fruits of pyrus communis had been collected from
Madanapalle, Chittoor District, Andhra pradesh, India.
The fruit was identified and authenticated by the
Botanist Dr. K. Madhava Chetty, Assistant Professor,
Department of botany, Sri Venkateswara University,
Tirupathi.
Preparation of extracts
The collected fruits were shade dried completely. The
dried fruit was then coarsely powdered and was sieved
(sieve # 60) to get uniform powdered. The powdered
materials were defatted with Petroleum ether by
maceration for 48 hours. The marc was dried and
successive extracted with solvent chloroform, ethyl
acetate, 80% ethanol and aqueous by maceration
process. Final compound was concentrated by vacuum
drying. The traces of the solvents were removed by
keeping the dried extracts in to desiccators.
Preliminary phytochemical screening
The different extracts of fruits of pyrus communis was
screened for the presence of various phyto constituents
like alkaloids, flavonoids, saponins, tannin, glycosides
[11].
Determination of total phenolic contents in the
extracts [12]
The concentration of total phenol in plant extracts was
determined using spectrophotometric method
(Singleton et al., 1999). The extracts in the
concentration of 1 mg/ml was used in the analysis. The
reaction mixture was prepared by mixing 0.5 ml
methanolic solution of extracts, 2.5 ml of 10% Folin-
Ciocalteu’s reagent dissolved in water and 2.5 ml 7.5%
NaHCO3. Blank was concomitantly prepared,
containing 0.5 ml methanol, 2.5 ml 10% Folin-
Ciocalteu’s reagent dissolved in water and 2.5 ml of
7.5% of NaHCO3. The samples were thereafter
incubated in a thermostat at 45o
C for 45 min. The
absorbance was determined using spectrophotometer at
765 nm. The samples were prepared in triplicate for
each analysis and the mean value of absorbance was
obtained. The same procedure was repeated for the
standard solution of gallic acid (10-100mg/ml) and the
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calibration line was construed. Based on the measured
absorbance, the concentration of phenolics was read
from the calibration line; then the content of phenolics
in extracts was expressed in terms of gallic acid
equivalent (mg of GA/g of extract).
Estimation total tannins [13]
The total content of tannin in different extracts of fruits
of pyrus communis was determined by folin denis
method(100g of sodium sulphate+20g of
phosphomalybdic acid +50 ml of phosphoric acid and
750ml of distilled water was refluxed or boiled for 2
hrs and make up the volume 1000 ml with distilled
water). The colorimetric estimation of tannin is based
on the measurement of blue colour formed by reduction
of phosphor tungsto malybdic acid by tannin like
compound in alkaline medium. 1g/ ml of extracts and
standard solution of tannic acid (10-100mg/ml) was
made up to 7.5 ml with distilled water. Then 0.5 ml of
Folin denis reagent and 1 ml of Na2 CO3 solution was
added. The volume was made up to 10 ml with distilled
water and absorbance was measured at 700 nm. The
total tannic acid content was expressed mg equivalent
of tannic acid per gram of extracts.
Determination of total flavonoids contents [14]
The total flavonoids content of each plant extract was
estimated as per Zhishenet al[9]. In-brief, each sample
(1.0ml) was mixed with 4ml of distilled water and
subsequently with 0.30ml of a NaNO2 solution (10%).
After 5 min, 0.30 ml of an AlCl3 solution (10%) w a s
added followed by 2.0 ml of NaOH solution (1%).
Immediately, after thorough mixing the absorbance was
measured at 510 nm versus the blank. Standard curve of
rutin was prepared(10-100mg/ml) and the results are
expressed as rutin equivalents (mg ruin/gm dried
extract).
Statistical analysis:
Experimental data are expressed as mean±standard
error of mean (SEM). Statistical analysis was
performed by two-way ANOVA followed by
bonferroni posttests method of multiple comparisons
was employed using Graphpad prism 5.0 software.
Data were considered significant at p < 0.001 &
p<0.01.
RESULTS
Preliminary phytochemical screening
The preliminary phytochemical analysis of different extracts of pyrus communis shows presence of steroid, flavonoids,
glycosides, tannin, alkaloids, phenolic compound, proteins and carbohydrate. (Table 1)
Table 1:Preliminary phytochemical screening different extract of Pyrus communis L.
S.No Constituents Tests Chloroform Ethyl acetate Ethanol Aqueous
1 Alkaloids Mayer’s test
Dragondraff’s test
Hager’s test
Wagner’s test
+
+
+
+
+
-
-
+
+
-
-
-
+
+
+
+
2 Sterols Burchard test
Salkowski’s
+
+
-
-
-
-
-
-
3 Carbohydrates Molisch’s test
Fehling’s test
Benedict’s test
Barfoed’s test
-
+
+
+
+
+
+
-
+
+
+
+
+
+
+
+
4 Glycosodes Legal test - + + +
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Kellerkiallani test
Borntrager’s test
-
-_
+
+
+
+
+
+
5 Fixed oils & Fats Spot test
Saponification test
-
-
-
-
-
-
-
-
6 Phenolic
Compounds
Ferric chloride - + + +
7 Proteins &
amino acids
Biuret test
Ninhydrin test
Millon’s test
Xanthoproteic test
Cysteine test
Tryptophan test
+
-
-
-
-
-
+
+
+
+
+
-
+
+
+
+
-
-
+
-
-
+
+
-
8 Terpenoids &
Saponins
Foam test
Haemolysis test
-
-
+
-
+
-
+
-
9 Tannins Gelatin test
Fecl3 test
Lead acetate test
-
+
-
+
+
+
+
+
+
+
-
+
10 Gums & mucilage Mucilage test
Hydrolytic test
-
-
+
-
-
-
+
+
11 Flavonoids Shinoda test Conc.H2SO4
lead acetate
-
-
-
+
+
+
+
+
+
+
+
-
Where + =present, - =absent
Figure 1: The concentration response curve of GA, Ru & TA and absorbance different extracts of Pyrus
communis L.
0
0.5
1
1.5
5 10 20 40 80 100
Absorbance
Concentration of mg/ml og GA, Ru & TA
Absorbance of GA Absorbance of gm/ml of C,EA, E, AEPC in ETP
Absorbance of Ru Absorbance of gm/ml of C,EA, E, AEPC in ERu
Absorbance of TA Absorbance of gm/ml of C,EA, E, AEPC in ETA
5. Velmurugan Chinnasamy, et al / Int. J. of Pharmacy and Analytical Research Vol-3(4) 2014 [384-390]
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Standard curve prepared was used for the
determination of total phenolic content and flavonoids
using different concentrations of Gallic acid, tannic
acid and rutin respectively. The total phenolic,
flavonoids and tannin content in different extracts of
Pyrus communis have been presented in table.
Observation shows that total phenol content
significantly (p<0.001) differ between the extracts.
The content significantly was highest in the ethyl
acetate extract compare to ethanol, aqueous and
chloroform extract. There is no significant variation in
flavonoid content between ethyl acetate and ethanol.
But it is significantly differ (p<0.001) from aqueous
and chloroform extracts. The flavonoid content in
aqueous extract significantly (P<0.001) differ from
the chloroform extract. Significantly (p<0.001) high
concentration of tannin found in ethyl acetate and ethanol
compared to aqueous and chloroform extracts. No significant
variation observed in tannin content between chloroform and
aqueous extracts.
Table 2: Total phenol, flavonoid and tannin content of different extracts of fruits of Pyrus communis L.
Extracts Total phenol mg GA/g of
extract
Total flavonoid mg Ru/g of
extract
Total tannin mg TA/g of
extract
Chloroform 8.38±0.17d
9.72±0.59h
6.28±1.57j
Ethyl
acetate
49.33±0.08a
54.77±0.41e
32.76±1.13i
Ethanol 46.63±0.12b
52.92±0.94e
29.52±2.34i
Aqueous 15.27±0.03c
16.92±0.38g
10.96±1.02j
Values are expressed as mean± SEM of three replicates.
Mean bearing same superscripted do not differ
significantly. Mean bearing different
superscripted differ significantly. The different
superscripted ‘a’values have significantly differ
(p<0.001) from’ b, c’& d. ‘b’ indicates significantly
differ (p<0.001) from ‘c & d’. ‘c’ indicates
significantly differ (p<0.001) from ‘c’. ‘e’ differ from
(p<0.001) ‘g & h’. ‘g’ differ from (p<0.001)‘h’. ‘i’
significantly differ from (p<0.001) ‘j’
Figure 2: Total phenol, flavonoid and tannin content of different extracts of fruits of Pyrus communis L.
Values are expressed as mean± SEM of three replicates.
Mean bearing same superscripted do not differ
significantly. Mean bearing different
superscripted differ significantly. The different
superscripted ‘a’values have significantly differ
(p<0.001) from’ b, c’& d. ‘b’ indicates significantly
differ (p<0.001) from ‘c & d’. ‘c’ indicates
significantly differ (p<0.001) from ‘c’. ‘e’ differ from
(p<0.001) ‘g & h’. ‘g’ differ from (p<0.001)‘h’. ‘i’
significantly differ from (p<0.001) ‘j’
0
10
20
30
40
50
60
CEPC EAEPC EEPC AEPC
Total phenol mg GA/g of extract Total flavonoid mg Ru/g of extract
Total tannin mg TA/g of extract
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DISCUSSION
These medicinal plants are rich sources for naturally
occurring antioxidants especially phenolic and
flavonoids contents. These agents have ability to
scavenge free radicals, super oxide and hydroxyl
radicals, etc thus they enhance immunity and
antioxidant defense of the body [15]
. Dietary
supplementation of these compounds reduces the
oxidative damage to cell membrane lipid, protein and
nucleic acid due strong quenching property of free
radicals [16]
.
For acceptance of medicinal plants into scientific
medicine, it is necessary that their effectiveness and
safety be evaluated and confirmed through active
ingredient testing. To maximize the extractive
capability of phenolic and flavonoids components
from plant material is considerably depended on the
type of solvent. Highest content of phenolic,
flavonoids and tannin in ethanolic and ethyl acetate
extract in comparison to other solvents used, make
this organic solvent (ethanol) an ideal and selective to
extract a great number of bioactive phenolic
compounds. Similarly, Mohammedi [17]
. Collagen
fibers treated with the plant flavonoid, catechin, have
been found to be stable. Such stabilization effect has
been shown to involve hydrogen bonding and
hydrophobic interactions [18]
.
Tannins are generally defined as naturally occurring
polyphenolic compounds of high molecular weight to
form complexes with the proteins. Tannins are
important source of protein in animals but unfortunately
the amounts of tannins that they contain vary widely
and largely unpredictably, and their effects on animals
range from beneficial to toxicity and death [19]
. The
toxic or anti-nutritional effects tend to occur in times of
stress when a very large proportion of the diet having
high concentration of tannins. Thus consumption of
foods naturally having antioxidant activity is the most
efficient way of combating such tissue injuries,
undesired transformations and preventing health risks
[20]
. Tannins are phenolic compounds that typically act
as astringents and are found in a variety of herbal
products used for wound healing. This astringent
property is responsible for wound contraction and
increased rate of epithelialization at the granulation
formation and scar remolding phases [21]
. Accordingly,
topical treatment with a tannin rich fraction of the bark
of Terminalia arjuna was found to demonstrate
significant increase in the tensile strength of the
incision wounds. The maximum tensile strength was
developed by tannins-fraction treated rats (719 g,
compared to the standard reference formulation
Betadine (609g) [22].
CONCLUSION
In present study the ethyl acetate and ethanolic extract
have high concentration of flavonoids, tannin and
phenolic concentration. Therefore, ethyl acetate and
ethanolic extract of Pyrus communis have greater
potential to produces more beneficial effects or
pharmacological important as compared to other
extracts.
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