Atomic absorption spectroscopy (AAS) is a technique used to determine the concentration of metallic elements in solutions. It works by vaporizing the sample into neutral atoms and measuring the absorption of light from a hollow cathode lamp at a specific wavelength. The intensity of absorbed light is directly proportional to the concentration of the element. AAS can detect low ppm levels of elements and is used to analyze biological fluids, foods, soils, and other materials. It has advantages over flame photometry in being less susceptible to cationic interferences. Examples of applications include determining levels of copper, zinc, and lead in various products and samples.

1. ATOMIC ABSORPTION
SPECTROSCOPY (AAS)
Presented By:
G.T.ROOPESHM.PHARM
Associate Professor
Dept. Of Pharma.Chemistry
BCP-ATP
EMAIL ID: roopeshpharma@gmail.com

2. ATOMIC ABSORPTION SPECTROSCOPY(AAS)
CONTENTS
PRINCIPLE
THEORY
 Instrumentation
AAS (SINGLE)
AAS (Double)
COMPARISON
Applications

3. Principle of AAS
When a solution of metallic salt is sprayed on to a flame,
fine droplets are formed. Due to the thermal energy' of the
flame, the solvent in the droplets evaporate. leaving a fine
residue, which are converted to neutral atoms.
The intensity of light absorbed by the neutral atoms is directly
proportional to the concentration of the element & obeys Beers law
over a Tide concentration range. The intensity of radiation absorbed
by neutral atoms is measured using. photometric detectors (Photo
tube or Photo multiplier tube).

4. Theory of AAS
INTRODUCTION
Atomic absorption spectroscopy deals with the
absorption of specific wavelengths of radiation by
neutral atoms in the ground state. (The UV
spectroscopy, where absorption of radiation by
molecules occur).
Neutral atoms are obtained by spraying the sample
solution of a burner (like in flame photometer). Specific
wavelength of radiation is generated by using a hollow
cathode lamp. For determination of every element
separate hollow cathode lamp is required.

5. Preparation of SAMPLE

6. Instrumentation

8. NEBULISER
NEBULISER

9. ATOMISER

11. GRAPHITE
TUBE
ATOMIZER
OR
ELECTRO
THERMAL
ATOMISER
GRAPHITE TUBE ATOMISER OR ELECTRO THERMAL ATOMISER

12. Detector:
The intensity of radiation absorbed by elements, in the UV or visible region (190-
900nm) can be detected using Photometric detectors (Photo tube or Photo
multiplier tube).
Readout Device:
The readout device is capable of displaying the absorption spectrum as well as the
absorbance at a specified wavelength (similar to UV spectroscopy). Beer's law is
obeyed over a wide concentration range.

13. INTERFERENCES
AAS is less liable to be affected by interferences, when compared to Flame Photometry. The
technique of AAS is especially free from Cationic interferences. This is because of
the absorption of sharp resonance lines from hollow cathode lamp.

14. Advantages & Disadvantages

15. Appücations of AAS:
AAS is mainly used only for quantitative analysis of various elements present in different
samples. It is not used for qualitative'analysis, since unless separate lamps are used, it is not
possible to identify various, elements present in the given sample.
CALIBRATION CURVE METHOD is used in quantitative analysis, where eh
various standard solutions of the element to be determined are prepared and the absorbance of
each solution is determined. A calibration curve of concentration of elements (Vs) absorbance is
made, from which concentration of the element in the sample solution is determined. Beer's law
is obeyed over a wide range of concentration. Low levels of detection such as 0.001 ppm of
elements are possible in quantiative analysis by AAS.
The following are some of the applications of AAS:
1. Estimation of trace elements in biological fluids (eg. blood, urine» etc). Estimation of
copper, Nickel and Zinc in food products (FOOD INDUSTRY)
2. Estimation of elements like Copper products.
3. Estimation of Magnesium, Zinc, etc in blood.
4. Estimation of Zinc in Zinc Insulin injection.
5. Estimation of Mercury in Thiomersal solution.
6. Estimation of lead in Calcium carbonate, petrol, etc.
7. Estimation of elements in soil samples, water supply, effluents, ceramics, etc.
8. Simultaneous multi component analysis.
9. Determination of lead in Petrol.
10. Determination of metallic elements in Biological materials.

17. COMPARISON

18. APPLICATIONS OF AAS (EXPERIMENTS)
1. DETERMINATION OF Vanadium in lubricating OIL.
2. Determination of Trace elements in Contaminated soil.
DETERMINATION OF Vanadium in lubricating OIL.
Theory

19. Procedure:

20. Determination of Trace elements in Contaminated soil.
PROCEDURE: