Applications of crispr cas system
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CRISPR-Cas is a genome editing technique derived from bacterial immune systems that allows for precise genomic modifications. The document discusses applications of CRISPR-Cas in plants, animals, and bacteria, including developing pest and disease resistant crops and livestock, modifying stem cells and embryos, targeting antibiotic resistant bacteria, and controlling gene expression.
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Crispr cas9
This document discusses the CRISPR-Cas9 genome editing technique. It begins with an overview of genome editing and provides a brief history. It then focuses on explaining CRISPR-Cas9, including its key components, how it was discovered as a natural bacterial immune system, and how it functions as a genomic tool. The document outlines the general CRISPR-Cas9 protocol and recent advances in the technique. It discusses applications in agriculture and for diseases. It also touches on advantages and limitations, as well as ethical issues. Two case studies are provided that demonstrate using CRISPR-Cas9 to modify genes in rice plants.
CRISPR: Discovery & Potential Applications
CRISPR is easily the best gene editing tool to date. For decades, scientists have been looking for a way to to perform precise changes to genetic sequences. In the past several years, researchers were able to exploit the immune systems of bacteria to edit the genome of other living cells. CRISPR is reported to have higher targeting efficiencies when compared to TALENs and Zinc Fingers. It is efficient, easy to use and cheap; making it a scalable genetic engineering tool that is highly desirable in various industry-wide applications.
Crispar
This document provides an overview of the CRISPR-Cas immune system in bacteria and its applications. It discusses:
1. The history and components of the CRISPR-Cas system, including Cas proteins, CRISPR RNA, and protospacer adjacent motifs.
2. The three main types of CRISPR-Cas systems and their mechanisms of targeting DNA or RNA.
3. How engineered versions of Cas9 can be used for targeted genome editing and modulation of gene expression in mammalian cells.
4. Applications of CRISPR-Cas in microbiology such as genetic engineering of bacteria, gene repression/activation using deactivated Cas9, and developing sequence-specific antimicrobials.
crispr cas 9
CRISPR-Cas9 is a gene editing technology that uses the bacterial immune system to cut DNA at specific locations. It allows researchers to understand, characterize, and control DNA. CRISPR-Cas9 uses an RNA-guided DNA endonuclease enzyme called Cas9 that is directed by guide RNA to cleave target DNA. It has numerous applications including modifying genes in plants and animals, developing disease resistant crops, and potentially curing genetic diseases in humans by precisely editing genes. While revolutionary, it also raises ethical concerns that must be considered and addressed.
SEMINAR ON CRISPR
1. CRISPR is a bacterial immune system that provides immunity against viruses. It works by matching DNA sequences from viruses to DNA spacers and cleaving invading DNA.
2. The type II CRISPR system is the most well studied and requires only three components - Cas9 protein, CRISPR RNA (crRNA), and trans-activating CRISPR RNA (tracrRNA) - to function. Combining crRNA and tracrRNA into a single RNA molecule called sgRNA was crucial for developing the CRISPR technique.
3. CRISPR technology has become a powerful genome editing tool due to its simplicity, high efficiency, low cost, and ease of use. It allows targeted
Crispr cas
This document summarizes Jai Kishan's presentation on gene manipulation techniques. It discusses conventional plant breeding, mutation breeding, transgenic plants, and several precise gene editing techniques like ZFN, TALENs, and CRISPR-Cas9. CRISPR-Cas systems provide adaptive immunity in bacteria and archaea by recognizing foreign DNA. The document then reviews the history and components of the CRISPR-Cas system and its applications in cancer therapy, viral diseases, bacterial infections, and genetic disorders. It also discusses challenges like delivery methods, mosaicism, immune responses, and off-target mutations. Key references on the discovery of CRISPR and its use in agriculture and as a genetic manipulation tool are presented.
CRISPR in crop Improvement, CRISPR/Cas Genome editing tool
This document discusses the use of CRISPR-Cas9 genome editing in crop improvement. It begins with an introduction to CRISPR-Cas9 and its mechanism of action. It then discusses the discovery of CRISPR and key scientists involved. Several case studies on using CRISPR to edit rice genes for disease resistance and hybrid seed production are summarized. Achievements using CRISPR in rice, horticulture crops, and other field crops are briefly outlined. The document concludes that CRISPR provides a simple and efficient tool for genome editing in plants.
Crispr/cas9 101
This document provides an overview of CRISPR/Cas9 genome editing. It discusses the history and limitations of prior genome engineering techniques like recombinant DNA and zinc finger nucleases. It then explains how CRISPR/Cas9 works as a RNA-guided DNA endonuclease and how this allows it to efficiently and specifically edit genomes. The document outlines several applications of CRISPR/Cas9 like generating knockout animals and cell lines. It also notes some concerns about using the technique for human genome editing.
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Crispr cas9
This document discusses the CRISPR-Cas9 genome editing technique. It begins with an overview of genome editing and provides a brief history. It then focuses on explaining CRISPR-Cas9, including its key components, how it was discovered as a natural bacterial immune system, and how it functions as a genomic tool. The document outlines the general CRISPR-Cas9 protocol and recent advances in the technique. It discusses applications in agriculture and for diseases. It also touches on advantages and limitations, as well as ethical issues. Two case studies are provided that demonstrate using CRISPR-Cas9 to modify genes in rice plants.
CRISPR: Discovery & Potential Applications
CRISPR is easily the best gene editing tool to date. For decades, scientists have been looking for a way to to perform precise changes to genetic sequences. In the past several years, researchers were able to exploit the immune systems of bacteria to edit the genome of other living cells. CRISPR is reported to have higher targeting efficiencies when compared to TALENs and Zinc Fingers. It is efficient, easy to use and cheap; making it a scalable genetic engineering tool that is highly desirable in various industry-wide applications.
Crispar
This document provides an overview of the CRISPR-Cas immune system in bacteria and its applications. It discusses:
1. The history and components of the CRISPR-Cas system, including Cas proteins, CRISPR RNA, and protospacer adjacent motifs.
2. The three main types of CRISPR-Cas systems and their mechanisms of targeting DNA or RNA.
3. How engineered versions of Cas9 can be used for targeted genome editing and modulation of gene expression in mammalian cells.
4. Applications of CRISPR-Cas in microbiology such as genetic engineering of bacteria, gene repression/activation using deactivated Cas9, and developing sequence-specific antimicrobials.
crispr cas 9
CRISPR-Cas9 is a gene editing technology that uses the bacterial immune system to cut DNA at specific locations. It allows researchers to understand, characterize, and control DNA. CRISPR-Cas9 uses an RNA-guided DNA endonuclease enzyme called Cas9 that is directed by guide RNA to cleave target DNA. It has numerous applications including modifying genes in plants and animals, developing disease resistant crops, and potentially curing genetic diseases in humans by precisely editing genes. While revolutionary, it also raises ethical concerns that must be considered and addressed.
SEMINAR ON CRISPR
1. CRISPR is a bacterial immune system that provides immunity against viruses. It works by matching DNA sequences from viruses to DNA spacers and cleaving invading DNA.
2. The type II CRISPR system is the most well studied and requires only three components - Cas9 protein, CRISPR RNA (crRNA), and trans-activating CRISPR RNA (tracrRNA) - to function. Combining crRNA and tracrRNA into a single RNA molecule called sgRNA was crucial for developing the CRISPR technique.
3. CRISPR technology has become a powerful genome editing tool due to its simplicity, high efficiency, low cost, and ease of use. It allows targeted
Crispr cas
This document summarizes Jai Kishan's presentation on gene manipulation techniques. It discusses conventional plant breeding, mutation breeding, transgenic plants, and several precise gene editing techniques like ZFN, TALENs, and CRISPR-Cas9. CRISPR-Cas systems provide adaptive immunity in bacteria and archaea by recognizing foreign DNA. The document then reviews the history and components of the CRISPR-Cas system and its applications in cancer therapy, viral diseases, bacterial infections, and genetic disorders. It also discusses challenges like delivery methods, mosaicism, immune responses, and off-target mutations. Key references on the discovery of CRISPR and its use in agriculture and as a genetic manipulation tool are presented.
CRISPR in crop Improvement, CRISPR/Cas Genome editing tool
This document discusses the use of CRISPR-Cas9 genome editing in crop improvement. It begins with an introduction to CRISPR-Cas9 and its mechanism of action. It then discusses the discovery of CRISPR and key scientists involved. Several case studies on using CRISPR to edit rice genes for disease resistance and hybrid seed production are summarized. Achievements using CRISPR in rice, horticulture crops, and other field crops are briefly outlined. The document concludes that CRISPR provides a simple and efficient tool for genome editing in plants.
Crispr/cas9 101
This document provides an overview of CRISPR/Cas9 genome editing. It discusses the history and limitations of prior genome engineering techniques like recombinant DNA and zinc finger nucleases. It then explains how CRISPR/Cas9 works as a RNA-guided DNA endonuclease and how this allows it to efficiently and specifically edit genomes. The document outlines several applications of CRISPR/Cas9 like generating knockout animals and cell lines. It also notes some concerns about using the technique for human genome editing.
Genome Editing with CRISPR-Cas9
CRISPR-Cas9 is a genome editing tool that is creating a buzz in the science world. It is faster, cheaper and more accurate than previous techniques of editing DNA and has a wide range of potential applications.
Crispr-Cas9 technology
Crispr-Cas9 system works on the concept of bacterial defence mechanism. The idea of which was replicated in eukaryotic cell in in- vitro condition by the researchers.
CRISPR-Cas9 system a tool for gene editing presentation
CRISPR Cas9 System for Gene Editing
The document summarizes CRISPR Cas9 gene editing. It discusses the timeline of CRISPR discoveries from 1987-2019. It describes the classification, structure, and mechanism of the CRISPR Cas9 system. Applications discussed include using CRISPR in bacteria, wheat, and to alter muscle mass in humans. Ethical concerns regarding uses in human embryos and potential for non-medical enhancement are also covered.
Review of CRISPR/Cas9
CRISPR/Cas9 is a powerful new technique for genome editing that allows DNA to be easily cut and modified. It involves using the Cas9 enzyme, guided by RNA, to create targeted double-stranded breaks in DNA which are then repaired, allowing the DNA sequence to be altered. This system was adapted from a bacterial immune system. CRISPR/Cas9 represents a major breakthrough as it is simpler, cheaper and more accurate than previous genome editing methods. It has already been used to edit genes in numerous organisms and holds promise for applications like correcting genetic diseases. However, off-target effects and ethical concerns surrounding its use in humans remain limitations that need to be addressed.
Crispr cas9 ( a overview)
this slide provides an insight on newly emerging genome editing technique CRISPR Cas9 mechanism and applications.
Crispr
This document discusses the CRISPR-Cas9 genome editing technology. It begins by explaining that genome editing involves inserting, deleting, or replacing DNA in a genome using engineered molecular scissors. It then describes CRISPR-Cas9 specifically, noting that it is a bacterial immune system that utilizes CRISPR sequences and Cas9 proteins to cut viral DNA. The document outlines the components of the CRISPR-Cas9 system, including guide RNAs that locate DNA sections and Cas9 proteins that modify the DNA. It also discusses applications of CRISPR-Cas9 such as curing genetic diseases and editing crops, as well as noting that further investigation is still needed to ensure its safe therapeutic use.
PRINCIPLE OF CRISPR GENOME EDITING
Genome editing with the CRISPR-Cas9 system has become one of the major tools in modern biotechnology. This slide share discusses the fundamentals in a simple, easy to understand format.
CRISPR/CAS9- THE GENE EDITING TOOL
The document provides an overview of the CRISPR/Cas9 gene editing technology. It discusses the history and components of the CRISPR system, how it works, applications in various fields like microbiology, biomedicine, agriculture, and therapeutics. Recent advances expand its use for transcriptional regulation, epigenetic editing, and live imaging. While powerful, it faces challenges like off-target effects that require further research to optimize its safe and ethical application.
Crispr
This document provides an overview of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and its role as an adaptive immune system in prokaryotes. It describes the components and function of the CRISPR-Cas system, including how it provides immunity against viruses and plasmids. Applications of CRISPR technology discussed include phage resistance in bacteria, gene regulation, and bacterial strain typing. Potential future uses involve harnessing CRISPR biology for applications like transcriptional control.
CRISPR
Gene editing using CRISPR was originally discovered as a bacterial immune system that provides resistance to viruses. CRISPR uses specialized DNA sequences and associated Cas proteins to create targeted double-strand breaks in DNA, allowing modification of genomes. The technique has rapidly advanced due to its simplicity and versatility compared to prior tools. CRISPR holds promise for treating genetic diseases, transplantation, biotechnology, disease models, and more. It has become a widely used research tool with many companies and publications emerging around its applications.
CRISPR: Opportunities and Challenges Webinar
CRISPR is a genome-editing technique that provides a simple yet versatile method for making targeted changes to the genome of living cells. Researchers have already applied CRISPR to reverse disease-causing mutations and to engineer pest-resistant crops.
In this CRISPR webinar, PreScouter will be joined by experts and researchers to review CRISPR's opportunities and challenges. We will touch upon key challenges, such as reducing off-target effects, as well as new advances set to overcome some of the current limitations, such as novel CRISPR systems. Additionally, we'll highlight potential first applications of the technology within medicine and agriculture.
This is a great opportunity to not only learn from experts about how this disruptive technology is changing gene editing but also ask your personal questions about CRISPR.
Moderator:
This CRISPR discussion will be moderated by Charles Wright, the Medical Project Architect at PreScouter.
Panelists:
John G. Doench, Ph.D., is Associate Director of the Genetic Perturbation Platform at the Broad Institute.
C. B. Gurumurthy (Guru), BVSC, MVSC, Ph.D. Exec. MBA, is an Assistant Professor at the Department of Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation, and he serves as the Director of UNMC Mouse Genome Engineering Core Facility at the University of Nebraska Medical Center.
Shuibing Chen is an Assistant Professor in the Department of Surgery and Biochemistry at Weill Cornell Medical College, New York.
Crispr cas 9
This document provides information about CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) including:
1) CRISPR is a genome editing technique that uses the Cas9 enzyme to cut DNA at a targeted location, allowing DNA inserts or modifications.
2) It was originally discovered as a bacterial immune system but is now the most widely used approach for genome engineering due to its simplicity, precision, and versatility.
3) There is an ongoing patent dispute between researchers Jennifer Doudna, Emmanuelle Charpentier, and Feng Zhang over who first demonstrated using CRISPR-Cas9 for genome editing in human cells.
Crispr cas9 scalpels and their application
This document provides information on CRISPR Cas9 genome editing. It discusses the history and discovery of CRISPR dating back to 1987. It describes the key components of the CRISPR Cas9 system including Cas9 proteins, CRISPR RNA, protospacers, and PAM sequences. The mechanisms of how CRISPR Cas9 edits genomes through double strand breaks is explained. Finally, applications of CRISPR Cas9 are summarized, including using it to correct genetic mutations causing diseases in animals and potential applications in humans.
Crispr cas
This document provides an overview of CRISPR/Cas9 genome editing. It discusses how CRISPR/Cas9 enables precise modification of DNA sequences, outlines the timeline of key discoveries in CRISPR research, and describes the molecular mechanism and potential applications of this technology, including in microbial research, crop improvement, and human gene therapy. It also notes some limitations of the CRISPR/Cas9 system and concludes by emphasizing the opportunities it provides to advance research and address challenges like food security.
CRISPR Cas9
Introduction to CRISPR Cas9 technology. View in slide show after downloading for better viewing. Description is minimal, but it will be worth going through the slides that are full of pictures, if you have a minimal understanding of CRISPR.
Prepared in Oct 2015
Genome Editing Introduction
Genome editing is a method of making specific changes to the DNA of a cell or organism. An enzyme scissors the DNA at a specific sequence, and when this is repaired by the cell, a change or ‘edit’ is made to the sequence.
https://www.creative-biolabs.com/gene-therapy/approaches-to-genome-editing.htm
Crispr cas9 technology
This document provides an overview of CRISPR-Cas9 technology. It defines CRISPR as DNA sequences in bacteria that contain snippets of viral DNA used to detect and destroy invading viruses. The CRISPR system contains Cas9 proteins that cut DNA at specific locations guided by RNA, and RNA guide molecules. It works by integrating viral DNA into the bacteria's CRISPR loci, which are then transcribed into RNA to guide Cas9 to cleave invading viral DNA. Applications of CRISPR include disease modeling, cancer research, and correcting mutations in human embryos.
Genome editing
genome editing is the emerging branch which includes the specific and precise engineering of genomic regions
Crispr technique
Genome editing uses engineered nucleases to insert, delete, or replace sections of the genome. CRISPR/Cas9 is a popular genome editing technique that uses guide RNA to direct nucleases to specific DNA sequences. While promising for treating disease, human germline editing raises ethical concerns. Early studies editing human embryos and non-viable embryos demonstrated proof-of-concept but had low efficiencies and off-target mutations. Later studies improved targeting and showed potential for correcting genetic defects. However, regulation is needed as the first claimed use of CRISPR to genetically edit human babies was unapproved.
Crispr/Cas 9
CRISPR/Cas9 is a genome editing technique that allows for highly specific modification of DNA. It involves a Cas9 protein guiding a customized RNA to a target location in the genome to cut DNA. Scientists adapted the natural CRISPR immune system found in bacteria for genome editing. CRISPR/Cas9 holds promise for treating genetic diseases and developing crops but also raises ethical concerns when applied to human germline cells due to heritable effects. Researchers continue improving targeting specificity and developing new Cas proteins for additional applications in genome editing and gene regulation.
the application of CRISPR/Cas9 system in genome editing
CRISPR-cas9 system is a new robust genome editing tool and also this is a really easy to use system.
کاربرد سیستم کریسپر در ویرایش ژنوم.ppt
NAFLD Poster
The document describes an experiment using CRISPR/Cas9 to introduce mutations into the PNPLA3 gene in HepG2 cells. Sequencing of genomic DNA and mRNA from the cells showed the presence of SNP1, which changes an amino acid, but not SNP2. The researcher will use this sequence as a template to generate four combinations of the SNPs in order to study their effects on fat accumulation when transfected into HepG2 cells. This could provide insight into how genetic variants influence susceptibility to NAFLD in different ethnic groups.
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Genome Editing with CRISPR-Cas9
CRISPR-Cas9 is a genome editing tool that is creating a buzz in the science world. It is faster, cheaper and more accurate than previous techniques of editing DNA and has a wide range of potential applications.
Crispr-Cas9 technology
Crispr-Cas9 system works on the concept of bacterial defence mechanism. The idea of which was replicated in eukaryotic cell in in- vitro condition by the researchers.
CRISPR-Cas9 system a tool for gene editing presentation
CRISPR Cas9 System for Gene Editing
The document summarizes CRISPR Cas9 gene editing. It discusses the timeline of CRISPR discoveries from 1987-2019. It describes the classification, structure, and mechanism of the CRISPR Cas9 system. Applications discussed include using CRISPR in bacteria, wheat, and to alter muscle mass in humans. Ethical concerns regarding uses in human embryos and potential for non-medical enhancement are also covered.
Review of CRISPR/Cas9
CRISPR/Cas9 is a powerful new technique for genome editing that allows DNA to be easily cut and modified. It involves using the Cas9 enzyme, guided by RNA, to create targeted double-stranded breaks in DNA which are then repaired, allowing the DNA sequence to be altered. This system was adapted from a bacterial immune system. CRISPR/Cas9 represents a major breakthrough as it is simpler, cheaper and more accurate than previous genome editing methods. It has already been used to edit genes in numerous organisms and holds promise for applications like correcting genetic diseases. However, off-target effects and ethical concerns surrounding its use in humans remain limitations that need to be addressed.
Crispr cas9 ( a overview)
this slide provides an insight on newly emerging genome editing technique CRISPR Cas9 mechanism and applications.
Crispr
This document discusses the CRISPR-Cas9 genome editing technology. It begins by explaining that genome editing involves inserting, deleting, or replacing DNA in a genome using engineered molecular scissors. It then describes CRISPR-Cas9 specifically, noting that it is a bacterial immune system that utilizes CRISPR sequences and Cas9 proteins to cut viral DNA. The document outlines the components of the CRISPR-Cas9 system, including guide RNAs that locate DNA sections and Cas9 proteins that modify the DNA. It also discusses applications of CRISPR-Cas9 such as curing genetic diseases and editing crops, as well as noting that further investigation is still needed to ensure its safe therapeutic use.
PRINCIPLE OF CRISPR GENOME EDITING
Genome editing with the CRISPR-Cas9 system has become one of the major tools in modern biotechnology. This slide share discusses the fundamentals in a simple, easy to understand format.
CRISPR/CAS9- THE GENE EDITING TOOL
The document provides an overview of the CRISPR/Cas9 gene editing technology. It discusses the history and components of the CRISPR system, how it works, applications in various fields like microbiology, biomedicine, agriculture, and therapeutics. Recent advances expand its use for transcriptional regulation, epigenetic editing, and live imaging. While powerful, it faces challenges like off-target effects that require further research to optimize its safe and ethical application.
Crispr
This document provides an overview of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and its role as an adaptive immune system in prokaryotes. It describes the components and function of the CRISPR-Cas system, including how it provides immunity against viruses and plasmids. Applications of CRISPR technology discussed include phage resistance in bacteria, gene regulation, and bacterial strain typing. Potential future uses involve harnessing CRISPR biology for applications like transcriptional control.
CRISPR
Gene editing using CRISPR was originally discovered as a bacterial immune system that provides resistance to viruses. CRISPR uses specialized DNA sequences and associated Cas proteins to create targeted double-strand breaks in DNA, allowing modification of genomes. The technique has rapidly advanced due to its simplicity and versatility compared to prior tools. CRISPR holds promise for treating genetic diseases, transplantation, biotechnology, disease models, and more. It has become a widely used research tool with many companies and publications emerging around its applications.
CRISPR: Opportunities and Challenges Webinar
CRISPR is a genome-editing technique that provides a simple yet versatile method for making targeted changes to the genome of living cells. Researchers have already applied CRISPR to reverse disease-causing mutations and to engineer pest-resistant crops.
In this CRISPR webinar, PreScouter will be joined by experts and researchers to review CRISPR's opportunities and challenges. We will touch upon key challenges, such as reducing off-target effects, as well as new advances set to overcome some of the current limitations, such as novel CRISPR systems. Additionally, we'll highlight potential first applications of the technology within medicine and agriculture.
This is a great opportunity to not only learn from experts about how this disruptive technology is changing gene editing but also ask your personal questions about CRISPR.
Moderator:
This CRISPR discussion will be moderated by Charles Wright, the Medical Project Architect at PreScouter.
Panelists:
John G. Doench, Ph.D., is Associate Director of the Genetic Perturbation Platform at the Broad Institute.
C. B. Gurumurthy (Guru), BVSC, MVSC, Ph.D. Exec. MBA, is an Assistant Professor at the Department of Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation, and he serves as the Director of UNMC Mouse Genome Engineering Core Facility at the University of Nebraska Medical Center.
Shuibing Chen is an Assistant Professor in the Department of Surgery and Biochemistry at Weill Cornell Medical College, New York.
Crispr cas 9
This document provides information about CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) including:
1) CRISPR is a genome editing technique that uses the Cas9 enzyme to cut DNA at a targeted location, allowing DNA inserts or modifications.
2) It was originally discovered as a bacterial immune system but is now the most widely used approach for genome engineering due to its simplicity, precision, and versatility.
3) There is an ongoing patent dispute between researchers Jennifer Doudna, Emmanuelle Charpentier, and Feng Zhang over who first demonstrated using CRISPR-Cas9 for genome editing in human cells.
Crispr cas9 scalpels and their application
This document provides information on CRISPR Cas9 genome editing. It discusses the history and discovery of CRISPR dating back to 1987. It describes the key components of the CRISPR Cas9 system including Cas9 proteins, CRISPR RNA, protospacers, and PAM sequences. The mechanisms of how CRISPR Cas9 edits genomes through double strand breaks is explained. Finally, applications of CRISPR Cas9 are summarized, including using it to correct genetic mutations causing diseases in animals and potential applications in humans.
Crispr cas
This document provides an overview of CRISPR/Cas9 genome editing. It discusses how CRISPR/Cas9 enables precise modification of DNA sequences, outlines the timeline of key discoveries in CRISPR research, and describes the molecular mechanism and potential applications of this technology, including in microbial research, crop improvement, and human gene therapy. It also notes some limitations of the CRISPR/Cas9 system and concludes by emphasizing the opportunities it provides to advance research and address challenges like food security.
CRISPR Cas9
Introduction to CRISPR Cas9 technology. View in slide show after downloading for better viewing. Description is minimal, but it will be worth going through the slides that are full of pictures, if you have a minimal understanding of CRISPR.
Prepared in Oct 2015
Genome Editing Introduction
Genome editing is a method of making specific changes to the DNA of a cell or organism. An enzyme scissors the DNA at a specific sequence, and when this is repaired by the cell, a change or ‘edit’ is made to the sequence.
https://www.creative-biolabs.com/gene-therapy/approaches-to-genome-editing.htm
Crispr cas9 technology
This document provides an overview of CRISPR-Cas9 technology. It defines CRISPR as DNA sequences in bacteria that contain snippets of viral DNA used to detect and destroy invading viruses. The CRISPR system contains Cas9 proteins that cut DNA at specific locations guided by RNA, and RNA guide molecules. It works by integrating viral DNA into the bacteria's CRISPR loci, which are then transcribed into RNA to guide Cas9 to cleave invading viral DNA. Applications of CRISPR include disease modeling, cancer research, and correcting mutations in human embryos.
Genome editing
genome editing is the emerging branch which includes the specific and precise engineering of genomic regions
Crispr technique
Genome editing uses engineered nucleases to insert, delete, or replace sections of the genome. CRISPR/Cas9 is a popular genome editing technique that uses guide RNA to direct nucleases to specific DNA sequences. While promising for treating disease, human germline editing raises ethical concerns. Early studies editing human embryos and non-viable embryos demonstrated proof-of-concept but had low efficiencies and off-target mutations. Later studies improved targeting and showed potential for correcting genetic defects. However, regulation is needed as the first claimed use of CRISPR to genetically edit human babies was unapproved.
Crispr/Cas 9
CRISPR/Cas9 is a genome editing technique that allows for highly specific modification of DNA. It involves a Cas9 protein guiding a customized RNA to a target location in the genome to cut DNA. Scientists adapted the natural CRISPR immune system found in bacteria for genome editing. CRISPR/Cas9 holds promise for treating genetic diseases and developing crops but also raises ethical concerns when applied to human germline cells due to heritable effects. Researchers continue improving targeting specificity and developing new Cas proteins for additional applications in genome editing and gene regulation.
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CRISPR-Cas9 system a tool for gene editing presentation
CRISPR-Cas9 system a tool for gene editing presentation
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the application of CRISPR/Cas9 system in genome editing
CRISPR-cas9 system is a new robust genome editing tool and also this is a really easy to use system.
کاربرد سیستم کریسپر در ویرایش ژنوم.ppt
NAFLD Poster
The document describes an experiment using CRISPR/Cas9 to introduce mutations into the PNPLA3 gene in HepG2 cells. Sequencing of genomic DNA and mRNA from the cells showed the presence of SNP1, which changes an amino acid, but not SNP2. The researcher will use this sequence as a template to generate four combinations of the SNPs in order to study their effects on fat accumulation when transfected into HepG2 cells. This could provide insight into how genetic variants influence susceptibility to NAFLD in different ethnic groups.
Werner's Syndrome Keara and Tianna BIO 408 505
CRISPR-CAS gene editing technology can be used to cure Werner's Syndrome by altering the DNA sequence. Werner's Syndrome is caused by a mutation in the WRN gene that produces premature aging. It is characterized by early development of diseases like cancer and atherosclerosis. CRISPR-CAS can delete the mutation responsible for the premature stop codon and insert a new sequence that allows for normal aging. This prevents transcription of the faulty RNA and eliminates symptoms of Werner's Syndrome.
CRISPR - gene-editing for everyone
Have you considered that protein over-expression or inefficient mRNA knockdown may be masking physiological effects in your assays? Increasingly scientists are moving to endogenous gene-editing to characterise the function of their genes of interest.
Dr Chris Thorne from Cambridge Biotech Horizon Discovery discusses the ground breaking gene-editing technology CRISPR. The simplicity of experimental design has led to rapid adoption of the technology across the scientific community. However, challenges remain.
This Slidedeck focuses specifically on implementing CRISPR experiments, and explore a number of key considerations crucial to maximising chances of targeting success, whether your goal is to generate a knock-out or a knock-in. Chris also takes a look at some of the alternative uses of CRISPR, including sgRNA genome wide synthetic lethality screens.
The slides aim to support those researchers either planning to or already using CRISPR gene-editing in their lab. Horizon Discovery have also recently launched a program aimed specifically at academic cell biologists to promote the adoption of CRISPR by offering FREE CRISPR Reagents for knock-out cell line generation - more information available here. http://www.horizondiscovery.com/what-we-do/discovery-toolbox/genassist-crispr--raav-genome-editing-tools
The Efficiency and Ethics of the CRISPR System in Human Embryos
This document summarizes research on the CRISPR/Cas9 system for genome editing in human embryos. It discusses efforts to understand DNA repair mechanisms after inducing double-strand breaks, reduce off-target mutations, and improve the specificity and efficiency of editing. While the technology shows promise, significant issues around off-target effects, mosaicism, and ethical concerns must still be addressed before any clinical applications. The document concludes that further basic research is needed to advance the field while also having open discussions on societal implications.
Crispr handbook 2015
This handbook describes CRISPR/Cas9 genome editing and other research applications for CRISPR technology.
Crispr cas ppt by ashish
The document summarizes the CRISPR-Cas immune system. It discusses how CRISPR-Cas systems use clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins to recognize and cleave foreign DNA. The systems acquire spacers from invading viruses and phages and integrate them into the CRISPR loci to develop immunity. The CRISPR-Cas system has three stages - adaptation, expression and interference. It also discusses applications of CRISPR-Cas9 in genome editing and modulation of gene expression.
Crispr cas: A new tool of genome editing
The document summarizes a presentation on CRISPR cas9, a new genome editing tool. It discusses the history of CRISPR, how CRISPR functions in bacteria, the classification and components of CRISPR systems, and the mechanism of CRISPR cas9. It then covers applications of CRISPR cas9 in genome editing, databases of CRISPR sequences, case studies using the technology, and future directions of CRISPR research.
Crispr
This document summarizes a seminar given on CRISPR technology. It discusses the history of CRISPR research from its discovery in bacteria in 1987 to its development as a genome editing tool in 2012. Key events and applications are outlined, including the founding of early biotech companies utilizing CRISPR. The core concepts of how CRISPR induces double strand breaks and DNA repair are explained. Recent advances in engineered Cas9 variants and discovery of Cpf1 are also summarized.
An Introduction to Crispr Genome Editing
In this short presentation, I make a case for doing genome editing vs some of the approaches that have gone before, describe some of the tools available, and the focus on CRISPR-Cas9, what it is, where it's come from and how it works.
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Transgenesis
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CRISPRCas_Biotechnology_GE_AmanNanavaty.pdf
Through this presentation, you can understand the basics of CRISPR-Cas systems and their importance in various fields of Biotech. The molecular mechanisms involved in CRISPR-Cas are clearly elucidated with appropriate diagrams. The applications of CRISPR-Cas Genetic Engineering in fields such as BioPharma, Plant Biotech and Bioproduction are listed out as well.
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Biotechnology and genatic
This document discusses the stages and applications of biotechnology. It begins by outlining the three stages of biotechnology development: ancient biotechnology related to food and shelter, classical biotechnology using fermentation for food production and medicine, and modern biotechnology using genetic engineering techniques. It then states that bacteria are useful in biotechnology due to their rapid reproduction and ability to produce complex molecules. The document goes on to discuss areas of biotechnology like genetic engineering, protein engineering, and plant/animal biotechnology. It provides examples of genetic engineering applications including producing recombinant proteins, transgenic plants and animals, animal cloning, and gene therapy to cure hereditary diseases.
Biopharming
1) Biopharming involves genetically engineering plants and animals to produce pharmaceuticals. It offers lower production costs compared to traditional methods.
2) Early examples include cows modified in 1990 to produce human lactoferrin and tobacco plants in 1992 producing human serum albumin.
3) Methods include stable transgenic plants with genes integrated into nuclear or chloroplast genomes or transient expression systems using viral or Agrobacterium vectors. Genes are inserted and plants are harvested for protein extraction and purification.
Tissue culture Applications For Genetic Improvement of Crop plants
Tissue culture techniques can be used for genetic improvement of plants. Some key applications include:
1. Meristem culture which allows production of virus-free plants by cultivating shoot meristems. This eliminates viruses that move through the vascular system.
2. Somaclonal variation which induces genetic variability that can be used for crop improvement traits like disease resistance. Selected variants require extensive field trials due to genetic instability.
3. Mutagenesis uses physical or chemical mutagens to induce desirable mutations and improve traits like disease resistance. However, frequency of desired mutations is low and screening large populations is required.
4. Embryo rescue promotes development of immature embryos, allowing interspecific hybridizations and
tissue culture
Tissue culture is the in vitro culture of plant cells, tissues, organs or whole plants under sterile conditions. It has various applications in plant breeding including producing virus-free plants through meristem culture, inducing genetic variability through somaclonal variation, generating mutations through mutagenesis, rescuing interspecific hybrid embryos, creating homozygous lines through haploids, developing synthetic seeds, conserving germplasm through cryopreservation, and transferring genes through genetic transformation. While tissue culture has advantages like rapid propagation and eliminating diseases, it also has disadvantages like requiring specialized facilities and expertise and not being optimized for all plant species.
Methods of gene transfer
To achieve genetic transformation in plants, we need the construction of a vector (genetic vehicle) which transports the genes of interest, flanked by the necessary controlling sequences i.e. promoter and terminator, and deliver the genes into the host plant.
TRANSGENIC TECHNOLOGY AND ITS APPLICATION.pptx
This document discusses transgenic technology and its applications. Transgenic techniques involve introducing foreign DNA into an organism's genome. Common methods to create transgenic animals include DNA microinjection, retrovirus-mediated gene transfer, sperm-mediated gene transfer, and embryonic stem cell-mediated gene transfer. Transgenic animals have applications in research, agriculture, medicine, and industry. While transgenic models have benefits like regulating gene expression and studying human diseases, they also present disadvantages such as animal suffering during research and potential environmental impacts.
1550891219118 large scale antigen production methods
This document discusses various methods for large scale production of antigens for vaccines. It begins by explaining that most current vaccines use killed or live attenuated organisms as the antigen. It then describes the evolution of vaccine types from whole organism to purified macromolecules to recombinant vectors. The document outlines the typical steps involved in vaccine production: generation of the antigen through growing pathogens, releasing and isolating the antigen, purification, addition of other components, packaging, and distribution. Finally, it discusses various platforms for antigen expression, including mammalian cells, yeast, bacteria, transgenic animals, baculovirus-insect cells, plants, microalgae, and production in fermentors.
Biotechnology (1).pptx
Biotechnology is the broad field of applying biological systems and living organisms to develop products and processes. Key techniques include artificial selection, induced mutagenesis, in vitro fertilization, DNA sequencing, cloning, and genetic engineering. DNA sequencing determines the order of nucleotide bases in DNA, and uses techniques like PCR and gel electrophoresis. Genetic engineering uses techniques to cut and join genetic material from different organisms to introduce into another and change its characteristics, creating genetically modified organisms (GMOs). Common applications include using microorganisms for industrial processes like food production and bioremediation.
Transformation system
This document discusses various plant transformation systems. It describes direct transformation techniques like microinjection, electroporation, silicon carbide-mediated and gene gun/biolistic transformation. It also discusses indirect transformation techniques like Agrobacterium tumefaciens-mediated and virus-mediated gene transfer. Agrobacterium tumefaciens is able to transfer T-DNA from its Ti plasmid into the plant genome. Viral vectors like caulimoviruses and geminiviruses are also used for plant transformation. The document further explains in planta transformation techniques like meristem transformation and floral dip method. It provides examples of transgenic crops developed using these techniques.
Genetica of Ideal traits in Biocontrol Agents for Introgressing and for Proge...
Ph. D. Scholar
Department of Entomology
Indira Gandhi Krishi Vishwavidyalaya
Raipur (Chhattisgarh)
Methods of Gene Transfer
Methods of Gene Transfer document discusses various methods of transferring genes into plants to create transgenic plants. It describes two main categories of gene transfer methods - physical and biological. Physical methods include microinjection, biolistics (gene gun), electroporation, and particle bombardment. Biological methods include Agrobacterium-mediated transformation, which involves using the bacteria Agrobacterium tumefaciens to transfer DNA into plant cells. The document also discusses transformation cassettes, selection of transgenic plants, analysis of transgenic plants, and some examples of commercially important transgenic crops like golden rice and Roundup Ready corn.
Transgenic animals
Transgenic animals are created through recombinant DNA technology by inserting foreign genes into the animal's genome. This is done to improve livestock, use animals as bioreactors for pharmaceutical production, and for research purposes. The main methods of creating transgenic animals are DNA microinjection, retrovirus-mediated gene transfer, embryonic stem cell transfer, and sperm-mediated gene transfer. Examples include transgenic cows that produce more nutritious milk, pigs with genes to reduce environmental pollution, and mice used widely as model organisms. While transgenic animals have benefits, there are also ethical concerns regarding animal welfare and unintended environmental impacts.
Future of innovations in transgenic animals
This document discusses the future of innovations in transgenic animals through genome editing and reproductive technologies. It outlines various techniques for creating transgenic animals such as embryo splitting, pronuclear injection, somatic cell nuclear transfer (SCNT), and molecular tools like recombinases, transposons, ZFNs, TALENs, and CRISPR/Cas9. Applications of transgenic animals include disease modeling, producing therapeutic agents, improving traits like growth and milk production, generating disease resistance, and xenotransplantation. While progress has been made, challenges remain around efficiency, mosaic mutations, and off-target effects that further research and regulation may help address.
B I O T E C H N O L O G Y A N D G E N E T I C E N G E N E E R I N G
Biotechnology uses techniques such as genetic engineering and cloning to modify organisms or their genes for useful applications like producing therapeutic proteins, developing genetically engineered vaccines, and creating transgenic plants with desirable traits. Techniques include transferring genes between organisms using vectors like plasmids or viruses, screening libraries of cloned DNA to find genes of interest, and using polymerase chain reaction to amplify specific DNA sequences. While biotechnology has potential benefits, it also faces controversies regarding long term safety and environmental impacts of genetically modified organisms.
Transgenic crops(GM crops)
With the advancement of biotechnology, Genetic engineering also become an important tool. Transgenic crops are the crops which are produced through genetic engineering by altering desirable traits into plant genome.
Biotechnology karnataka puc
Biotechnology can be summarized as follows:
1. It uses living organisms or their components to develop useful products. This includes genetically modifying microbes, plants and animals.
2. Key branches include genetic engineering, tissue culture, DNA fingerprinting and gene therapy. Genetic engineering is used to create GMOs by transferring genes between organisms.
3. Important tools for genetic engineering include vectors, restriction enzymes, DNA ligases, and host cells used to replicate recombinant DNA. This allows genes of interest to be isolated and transferred to create GMOs.
Similar to Applications of crispr cas system (20)
Tissue culture Applications For Genetic Improvement of Crop plants
Tissue culture Applications For Genetic Improvement of Crop plants
1550891219118 large scale antigen production methods
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B I O T E C H N O L O G Y A N D G E N E T I C E N G E N E E R I N G
B I O T E C H N O L O G Y A N D G E N E T I C E N G E N E E R I N G
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在线办理(salfor毕业证书)索尔福德大学毕业证毕业完成信一模一样
学校原件一模一样【微信：741003700 】《(salfor毕业证书)索尔福德大学毕业证》【微信：741003700 】学位证，留信认证（真实可查，永久存档）原件一模一样纸张工艺/offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原。
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
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留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
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8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
Thornton ESPP slides UK WW Network 4_6_24.pdf
ESPP presentation to EU Waste Water Network, 4th June 2024 “EU policies driving nutrient removal and recycling
and the revised UWWTD (Urban Waste Water Treatment Directive)”
Remote Sensing and Computational, Evolutionary, Supercomputing, and Intellige...
Remote Sensing and Computational, Evolutionary, Supercomputing, and Intellige...University of Maribor
Slides from talk:
Aleš Zamuda: Remote Sensing and Computational, Evolutionary, Supercomputing, and Intelligent Systems.
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Inter-Society Networking Panel GRSS/MTT-S/CIS Panel Session: Promoting Connection and Cooperation
https://www.etran.rs/2024/en/home-english/Applied Science: Thermodynamics, Laws & Methodology.pdf
When I was asked to give a companion lecture in support of ‘The Philosophy of Science’ (https://shorturl.at/4pUXz) I decided not to walk through the detail of the many methodologies in order of use. Instead, I chose to employ a long standing, and ongoing, scientific development as an exemplar. And so, I chose the ever evolving story of Thermodynamics as a scientific investigation at its best.
Conducted over a period of >200 years, Thermodynamics R&D, and application, benefitted from the highest levels of professionalism, collaboration, and technical thoroughness. New layers of application, methodology, and practice were made possible by the progressive advance of technology. In turn, this has seen measurement and modelling accuracy continually improved at a micro and macro level.
Perhaps most importantly, Thermodynamics rapidly became a primary tool in the advance of applied science/engineering/technology, spanning micro-tech, to aerospace and cosmology. I can think of no better a story to illustrate the breadth of scientific methodologies and applications at their best.
THEMATIC APPERCEPTION TEST(TAT) cognitive abilities, creativity, and critic...
THEMATIC APPERCEPTION TEST(TAT) cognitive abilities, creativity, and critic...Abdul Wali Khan University Mardan,kP,Pakistan
hematic appreciation test is a psychological assessment tool used to measure an individual's appreciation and understanding of specific themes or topics. This test helps to evaluate an individual's ability to connect different ideas and concepts within a given theme, as well as their overall comprehension and interpretation skills. The results of the test can provide valuable insights into an individual's cognitive abilities, creativity, and critical thinking skills20240520 Planning a Circuit Simulator in JavaScript.pptx
Evaporation step counter work. I have done a physical experiment.
(Work in progress.)
Authoring a personal GPT for your research and practice: How we created the Q...
Thematic analysis in qualitative research is a time-consuming and systematic task, typically done using teams. Team members must ground their activities on common understandings of the major concepts underlying the thematic analysis, and define criteria for its development. However, conceptual misunderstandings, equivocations, and lack of adherence to criteria are challenges to the quality and speed of this process. Given the distributed and uncertain nature of this process, we wondered if the tasks in thematic analysis could be supported by readily available artificial intelligence chatbots. Our early efforts point to potential benefits: not just saving time in the coding process but better adherence to criteria and grounding, by increasing triangulation between humans and artificial intelligence. This tutorial will provide a description and demonstration of the process we followed, as two academic researchers, to develop a custom ChatGPT to assist with qualitative coding in the thematic data analysis process of immersive learning accounts in a survey of the academic literature: QUAL-E Immersive Learning Thematic Analysis Helper. In the hands-on time, participants will try out QUAL-E and develop their ideas for their own qualitative coding ChatGPT. Participants that have the paid ChatGPT Plus subscription can create a draft of their assistants. The organizers will provide course materials and slide deck that participants will be able to utilize to continue development of their custom GPT. The paid subscription to ChatGPT Plus is not required to participate in this workshop, just for trying out personal GPTs during it.
Immersive Learning That Works: Research Grounding and Paths Forward
We will metaverse into the essence of immersive learning, into its three dimensions and conceptual models. This approach encompasses elements from teaching methodologies to social involvement, through organizational concerns and technologies. Challenging the perception of learning as knowledge transfer, we introduce a 'Uses, Practices & Strategies' model operationalized by the 'Immersive Learning Brain' and ‘Immersion Cube’ frameworks. This approach offers a comprehensive guide through the intricacies of immersive educational experiences and spotlighting research frontiers, along the immersion dimensions of system, narrative, and agency. Our discourse extends to stakeholders beyond the academic sphere, addressing the interests of technologists, instructional designers, and policymakers. We span various contexts, from formal education to organizational transformation to the new horizon of an AI-pervasive society. This keynote aims to unite the iLRN community in a collaborative journey towards a future where immersive learning research and practice coalesce, paving the way for innovative educational research and practice landscapes.
Phenomics assisted breeding in crop improvement
As the population is increasing and will reach about 9 billion upto 2050. Also due to climate change, it is difficult to meet the food requirement of such a large population. Facing the challenges presented by resource shortages, climate
change, and increasing global population, crop yield and quality need to be improved in a sustainable way over the coming decades. Genetic improvement by breeding is the best way to increase crop productivity. With the rapid progression of functional
genomics, an increasing number of crop genomes have been sequenced and dozens of genes influencing key agronomic traits have been identified. However, current genome sequence information has not been adequately exploited for understanding
the complex characteristics of multiple gene, owing to a lack of crop phenotypic data. Efficient, automatic, and accurate technologies and platforms that can capture phenotypic data that can
be linked to genomics information for crop improvement at all growth stages have become as important as genotyping. Thus,
high-throughput phenotyping has become the major bottleneck restricting crop breeding. Plant phenomics has been defined as the high-throughput, accurate acquisition and analysis of multi-dimensional phenotypes
during crop growing stages at the organism level, including the cell, tissue, organ, individual plant, plot, and field levels. With the rapid development of novel sensors, imaging technology,
and analysis methods, numerous infrastructure platforms have been developed for phenotyping.
The debris of the ‘last major merger’ is dynamically young
The Milky Way’s (MW) inner stellar halo contains an [Fe/H]-rich component with highly eccentric orbits, often referred to as the
‘last major merger.’ Hypotheses for the origin of this component include Gaia-Sausage/Enceladus (GSE), where the progenitor
collided with the MW proto-disc 8–11 Gyr ago, and the Virgo Radial Merger (VRM), where the progenitor collided with the
MW disc within the last 3 Gyr. These two scenarios make different predictions about observable structure in local phase space,
because the morphology of debris depends on how long it has had to phase mix. The recently identified phase-space folds in Gaia
DR3 have positive caustic velocities, making them fundamentally different than the phase-mixed chevrons found in simulations
at late times. Roughly 20 per cent of the stars in the prograde local stellar halo are associated with the observed caustics. Based
on a simple phase-mixing model, the observed number of caustics are consistent with a merger that occurred 1–2 Gyr ago.
We also compare the observed phase-space distribution to FIRE-2 Latte simulations of GSE-like mergers, using a quantitative
measurement of phase mixing (2D causticality). The observed local phase-space distribution best matches the simulated data
1–2 Gyr after collision, and certainly not later than 3 Gyr. This is further evidence that the progenitor of the ‘last major merger’
did not collide with the MW proto-disc at early times, as is thought for the GSE, but instead collided with the MW disc within
the last few Gyr, consistent with the body of work surrounding the VRM.
Randomised Optimisation Algorithms in DAPHNE
Slides from talk:
Aleš Zamuda: Randomised Optimisation Algorithms in DAPHNE .
Austrian-Slovenian HPC Meeting 2024 – ASHPC24, Seeblickhotel Grundlsee in Austria, 10–13 June 2024
https://ashpc.eu/
Compexometric titration/Chelatorphy titration/chelating titration
Classification
Metal ion ion indicators
Masking and demasking reagents
Estimation of Magnisium sulphate
Calcium gluconate
Complexometric Titration/ chelatometry titration/chelating titration, introduction, Types-
1.Direct Titration
2.Back Titration
3.Replacement Titration
4.Indirect Titration
Masking agent, Demasking agents
formation of complex
comparition between masking and demasking agents,
Indicators/Metal ion indicators/ Metallochromic indicators/pM indicators,
Visual Technique,PM indicators (metallochromic), Indicators of pH, Redox Indicators
Instrumental Techniques-Photometry
Potentiometry
Miscellaneous methods.
Complex titration with EDTA.
如何办理(uvic毕业证书)维多利亚大学毕业证本科学位证书原版一模一样
原版纸张【微信：741003700 】【(uvic毕业证书)维多利亚大学毕业证】【微信：741003700 】学位证，留信认证（真实可查，永久存档）offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原海外各大学 Bachelor Diploma degree, Master Degree Diploma
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
bordetella pertussis.................................ppt
Bordettela is a gram negative cocobacilli spread by air born drop let
Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...
The pygmy halfbeak Dermogenys colletei, is known for its viviparous nature, this presents an intriguing case of relatively low fecundity, raising questions about potential compensatory reproductive strategies employed by this species. Our study delves into the examination of fecundity and the Gonadosomatic Index (GSI) in the Pygmy Halfbeak, D. colletei (Meisner, 2001), an intriguing viviparous fish indigenous to Sarawak, Borneo. We hypothesize that the Pygmy halfbeak, D. colletei, may exhibit unique reproductive adaptations to offset its low fecundity, thus enhancing its survival and fitness. To address this, we conducted a comprehensive study utilizing 28 mature female specimens of D. colletei, carefully measuring fecundity and GSI to shed light on the reproductive adaptations of this species. Our findings reveal that D. colletei indeed exhibits low fecundity, with a mean of 16.76 ± 2.01, and a mean GSI of 12.83 ± 1.27, providing crucial insights into the reproductive mechanisms at play in this species. These results underscore the existence of unique reproductive strategies in D. colletei, enabling its adaptation and persistence in Borneo's diverse aquatic ecosystems, and call for further ecological research to elucidate these mechanisms. This study lends to a better understanding of viviparous fish in Borneo and contributes to the broader field of aquatic ecology, enhancing our knowledge of species adaptations to unique ecological challenges.
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bordetella pertussis.................................ppt
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Applications of crispr cas system
- 1. Applications of CRISPR-CAS System -By Sarika Dengle Roll no: 10487
- 2. INTRODUCTION • CRISPR- Clustered Regularly Interspaced Short Palindromic Repeats. • Recently developed system. • Genome editing technique derived from the bacterial immune system. • Precise genomic modifications. • Gene sequences can be included from a range of donors
- 3. Applications in Plants • GE Crops: • Better Pest Resistance • Enhanced nutritional value • Eg : White Button Mushrooms, Waxy Corn Hybrid. • Crops with better root systems • Haploid Plants • Chemicals and Bio-materials
- 4. Applications In Animals • Genetically engineer Embryonic Stem cells. • Modify primate embryos • Disease resistant Animals • Eg: Pigs resistant to viral diseases, cattle resistant to trypanosome parasites. • Producing animals to give more yield. • Designer babies and animals.
- 5. Applications in Bacteria • CRISPR -CAS - Defense mechanism in 40% of Bacterial species. • This system on itself is lethal due to the NHEJ pathway being ineffective. • Targeting Antibiotic Resistant Bacteria • Genome editing and Gene expression