This presentation provides an overview of antigens, antibodies, and immunoglobulins. It discusses the structure and types of antibodies, including IgG, IgA, IgM, IgD, and IgE. The properties and functions of immunoglobulins are also summarized. Key points covered include the Y-shaped structure of antibodies, the roles of antibodies in agglutination, precipitation, and activation of the immune response, and the use of antibodies and immunoglobulins to identify and respond to foreign antigens in the body.
Antigen-antibody interaction, or antigen-antibody reaction, is a specific chemical interaction between antibodies produced by B cells of the white blood cells and antigens during immune reaction. ... The specificity of the binding is due to specific chemical constitution of each antibody
ANTIGEN, HAPTEN, ALL TYPES OF ANTIGENS, IMMUNOGEN , ATTRIBUTES OF ANTIGENICITY, DETERMINANTS OF ANTIGENICITY,
IMMUNOLOGY KUBY, MEDICAL MICROBIOLOGY & IMMUNOLOGY OF PANIKER , LIPPINCOTT'S IMMUNOLOGY, OTHER SOURCES.
antibodies are a large proteins. based on electrophorosis and centrifugation anti bodies are mainly five types .these are protects on human body from various microorganisms.
Antigens are the substances which induce specific immune reactions in the body.
Antigens include molecules such as proteins, nucleoproteins, polysaccharides, lipoprotein and some glycolipids.
The ability of a molecule to function as an antigen depends on its size, structural complexity, chemical nature, and degree of foreignness to the host.
Types of antigens
Antigens are of two types:
1. Autoantigens or self antigens present on the body’s own cells such as ‘A’ antigen and ‘B’ antigen in RBCs.
2. Foreign antigen s or non-self antigens that enter the body from outside.
Following are non-self antigens:
1. Receptors on the cell membrane of microbial organisms such as bacteria, viruses and fungi.
2. Toxins from microbial organisms.
3. Materials from transplanted organs or incompatible blood cells.
4. Allergens or allergic substances like pollen grains.
Antigen-antibody interaction, or antigen-antibody reaction, is a specific chemical interaction between antibodies produced by B cells of the white blood cells and antigens during immune reaction. ... The specificity of the binding is due to specific chemical constitution of each antibody
ANTIGEN, HAPTEN, ALL TYPES OF ANTIGENS, IMMUNOGEN , ATTRIBUTES OF ANTIGENICITY, DETERMINANTS OF ANTIGENICITY,
IMMUNOLOGY KUBY, MEDICAL MICROBIOLOGY & IMMUNOLOGY OF PANIKER , LIPPINCOTT'S IMMUNOLOGY, OTHER SOURCES.
antibodies are a large proteins. based on electrophorosis and centrifugation anti bodies are mainly five types .these are protects on human body from various microorganisms.
Antigens are the substances which induce specific immune reactions in the body.
Antigens include molecules such as proteins, nucleoproteins, polysaccharides, lipoprotein and some glycolipids.
The ability of a molecule to function as an antigen depends on its size, structural complexity, chemical nature, and degree of foreignness to the host.
Types of antigens
Antigens are of two types:
1. Autoantigens or self antigens present on the body’s own cells such as ‘A’ antigen and ‘B’ antigen in RBCs.
2. Foreign antigen s or non-self antigens that enter the body from outside.
Following are non-self antigens:
1. Receptors on the cell membrane of microbial organisms such as bacteria, viruses and fungi.
2. Toxins from microbial organisms.
3. Materials from transplanted organs or incompatible blood cells.
4. Allergens or allergic substances like pollen grains.
i am discuss about ,
1] INTRODUCTION OF ANTIBODIES
2] HISTORY OF ANTIBODIES
3] STRUCTURE OF ANTIBODIES
4] IMMUNOGLOBULIN DOMAINS
5] HEAVY CHAIN
6] LIGHT CHAIN
7] CLASSES OF ANTIBODIES
& ITS FUNCTIONS.
Enzyme Linked Immunosorbent Assay (ELISA) is a very sensitive immunochemical technique which is used to access the presence of specific protein (antigen or antibody) in the given sample and it’s quantification.
It is also called solid-phase enzyme immunoassay as it employs an enzyme linked antigen or antibody as a marker for the detection of specific protein.
ELISA has been used as a diagnostic tool in medicine, plant pathology and in the food industry as a quality control check.
Immunology is the study of the immune system and is a very important branch of the medical and biological sciences. The immune system protects us from infection through
i am discuss about ,
1] INTRODUCTION OF ANTIBODIES
2] HISTORY OF ANTIBODIES
3] STRUCTURE OF ANTIBODIES
4] IMMUNOGLOBULIN DOMAINS
5] HEAVY CHAIN
6] LIGHT CHAIN
7] CLASSES OF ANTIBODIES
& ITS FUNCTIONS.
Enzyme Linked Immunosorbent Assay (ELISA) is a very sensitive immunochemical technique which is used to access the presence of specific protein (antigen or antibody) in the given sample and it’s quantification.
It is also called solid-phase enzyme immunoassay as it employs an enzyme linked antigen or antibody as a marker for the detection of specific protein.
ELISA has been used as a diagnostic tool in medicine, plant pathology and in the food industry as a quality control check.
Immunology is the study of the immune system and is a very important branch of the medical and biological sciences. The immune system protects us from infection through
What is an Antibody?Immunoglobulins: Classes and Sub classesvarinder kumar
Forms
History
Immunoglobulins: Classes and Sub classes
Epitope
Antibodies structure
Antibody–antigen interactions
Function
Medical Applications
Regulations
Preclinical studies
Structure prediction
Antibody mimetic
OUTCOMES
By the end of this session student should be able to know
The structure of antibody
Immunoglobulin classes
Monoclonal antibodies VS polyclonal
INTRODUCTION
Antibodies are globulin proteins (immunoglobulins [Ig]) that react specifically with the antigen that stimulated their production.
They make up about 20% of the protein in blood plasma. Blood contains three types of globulins,
alpha,
beta,
gamma,
Antibodies are gamma globulins.
INTRODUCTION
There are five classes of antibodies:
1. IgG,
2. IgM,
3. IgA,
4. IgD,
5. IgE
Antibodies are subdivided into these five classes based on differences in their heavy chains.
ROLE OF ANTIBODIES
The most important functions of antibodies are to
neutralize toxins and viruses,
to opsonize microbes
so they are more easily phagocytosed, to activate complement, and to prevent the attachment of microbes to mucosal surfaces.
In addition to these functions, antibodies have a catalytic (enzymatic) capability
Antibody Type
IgA
IgD
IgE
IgG
IgM
Function
Found in saliva, tears, mucus, breast milk and intestinal fluid, IgA protects against ingested and inhaled pathogens.
This antibody is found on the surface of your B cells. Though its exact function is unclear, experts think that IgD supports B cell maturation and activation.
Found mainly in the skin, lungs and mucus membranes, IgE antibodies cause your mast cells (a type of white blood cell) to release histamine and other chemicals into your bloodstream. IgE antibodies are helpful for fighting off allergic reactions.
This is the most common antibody, making up approximately 70% to 75% of all immunoglobulins in your body. It’s found mainly in blood and tissue fluids. IgG antibodies help protect your body from viral and bacterial infections.
Found in your blood and lymph system, IgM antibodies act as the first line of defense against infections. They also play a large role in immune regulation.
MONOCLONAL VS POLYCLONAL
A. Polyclonal antibodies contain a heterologous mixture of IgGs against the whole antigen
B. monoclonal antibodies are composed of a single IgG against one epitope.
Polyclonal antibodies
Monoclonal antibodies
Refer to a mixture of immunoglobulin molecules that are secreted against a particular antigen.
Refer to a homogenous population of antibodies that are produced by a single clone of plasma B cells.
Produced by different clones of plasma B cells.
Produced by the same clone of plasma B cells.
A heterogeneous antibody population.
A homogenous antibody population.
Interact with different epitopes on the same antigen.
Interact with a particular epitope on the antigen.
STRUCTURE OF ANTIBODY
Immunoglobulins are glycoproteins made up of
1. light (L)
2. heavy (H) polypeptide chains.
The terms light and heavy refer to molecular weight
STRUCTURE OF ANTIBODY
The simplest antibody molecule has a Y shape consist of
This presentation clearly describes what are immunoglobulins, their types, structure and how they get diversified into different isotopes to fight with foreign antigens.
Antibody (Ab) also known as Immunoglobulin (Ig) is the large Y shaped protein produced by the body’s immune system when it detects harmful substances, called antigens like bacteria and viruses. The production of antibodies is a major function of the immune system and is carried out by a type of white blood cell called a B cell (B lymphocyte), differentiated B cells called plasma cells. The produced antibodies bind to specific antigens express in external factors and cancer cells.
Another name of anti-body is immunoglobulins . Anti-body is the glycoprotein which is produce by Bcell and which are responsible to bind antigen with higher specifity and affinity. Anti-body is mainly
distrusted in body fluid and also present surface of some cell.
Autoimmune disease HEMOLYTIC ANEMIA AND DIABETESArchanaSoni3
An autoimmune disease is a condition in which your immune system mistakenly attacks your body.
The immune system normally guards against germs like bacteria and viruses. When it senses these foreign invaders, it sends out an army of fighter cells to attack them.
Normally, the immune system can tell the difference between foreign cells and your own cells.
In an autoimmune disease, the immune system mistakes part of your body — like your joints or skin — as foreign. It releases proteins called autoantibodies that attack healthy cells.
Some autoimmune diseases target only one organ. Type 1 diabetes damages the pancreas. Other diseases, like lupus, affect the whole body.
endocytosis and exocytosis is a procss of cell eating and drinnking. it is a mazor tool for self defence to an individual cell. there are some molecular mechanism for this process described in given notes.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
This pdf is about the Schizophrenia.
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4. ANTIGEN
A substance that can produce a specific immune response when it is introduced into
The tissue of an animals and that can react specifically with antibodies or sensitised
Cells is known as an antigen.
the ability of an antigen to produce an immune response and to react with the
Product an is known as antigenicity. The ability of a material to induce an immune
Response is referred to as immunogenicity and such materials are known as immuno-
Gens.
EPITOPES AND PARATOPES
The part of the antigen with which the antibody reacts is known as the epitope or
Antigenic determinant . The portion of the antibody molecule that binds to the epitope
Is called the paratope.
The epitope determinant is bound to the specific antibody by
Non-covalent bounds and their binding is more or less three dimensional and may
4Be similar to a lock and key fit
5. 2.CHEMICAL NATURE:-
Most of the naturally occurring antigens are proteins and
Polysaccharides and these antigens are found to be compa-
Ratively more antigenic than lipids and nucleic acids.
Proteins are better antigenic when compared to polysacha-
Rides of the same size.
3.SOLUBILITY:-
The nonantigenicity of synthetic polymers also attributed to their
insolubility in body fluids and any substance which is not con-
Verted to soluble forms by tissue enzymes are also not antigenic
For example pneumococcal polysaccharides are greater immuno-
Genic for mice than for rabbits because of the greater hydrolytic
Activity of mouse liver enzymes for these polysaccharides.
5
SOME ESSENTIAL FACTORS FOR ANTIGENICITY
1.SIZE:- The molecular size of an antigen has a direct relation to
antigenicity Very large molecules such as hemocyanin
(6,000 mol.wt.) thyroglobulin(669 mol.wt.) and tetanus
toxin(55,000 mol. Wt.) are excellent antigens .
6. 4.FOREIGNNESS:-
To be antigenic the macromolecule must come from a foreign
Source . Antigens from related species are less antigenic than of
Unrelated species and antigens from members of same species
Are less antigenic than that of other species. For example plant
Proteins are good antigens in animals whereas duck serum
Proteins are not good antigens for chick.
CROSS REACTIVE ANTIGENS:-
Antigen antibody reaction is specific and th
Specificity is determined by spatial configuration of the specificity is determined by
Spatial configuration of the antigenic determinant group .but the antigen specificity
Is not absolute . Cross reactions can occur between antigens which bear stereochemic
Similarities. Cross reacting antigen is one which is capable of binding to antibody
Produced in response to a different antigen.
6
7. INTRODUCTION:--
1.Immunoglobulin are immunologically active serum
proteins formed in response to a antigen and react specifically with that
antigen.
2. A blood protein produced in response to and counteracting a specific
antigen.
3. Antibodies combine chemically with substance which the body
recognizes as alien, such as bacteria , viruses, and foreign substances in
the blood.
4. Antibody are Y-shaped proteins which play a pivotal role in our
immunity against bacterial and viral infection.
7
8. HISTORY:-
•Rodney porter (1962) :-proposed the basic structure of
immunoglobulin. Immunoglobulin is glycoprotein.
•Georeges kohler and cesar milstein (1975):- signally the start
of the modern of antibody research and discovery.
• Gerald edelman(1959):- indenpendently published the
molecular structure of antibody .for which they were later
joinyly awarded the nobel prize.
8
10. TYPES OF ANTIBODY :--
1.(IMMUNOGLOBULINE A ) IgA
2.(IMMUNOGLOBULINE D ) IgD
3.(IMMUNOGLOBULINE E ) IgE
4.(IMMUNOGLOBULINE G ) IgG
5.(IMMUNOGLOBULINE M ) IgM
10
11. 1.(IMMUNOGLOBULIN G ) IgG :--
IgG is immunoglobulinG. It is an antibody
It is a glycoprotein it is Y shaped. It is a relatively stable four
polypeptide chained
Molecule formed of 2 light chains(kappa or lemda ) and 2 heavy
chains of (gamma)
.There are 4 antigenicity distinct subclasses of igG namely igG1 , igG2,
igG3 , igG4.
The molecular weight of IgG is 150,000 and its sedimentation is 7s.
11
12. 2.( IMMUNOGLOBULINE A ) IgA:--
IgA is an antibody. IgA is found in serum. IgA is glycoprotein . It is Y-
shaped.
It is a two types , namely serum IgA and secretory IgA , Secretory IgA is
seen in the external seromucous secretion of the respiratory, gastro
intestinal and urinogenital tracts .
The IgA is synthesised by plasma cells in the lamina propria of mucous
membran and intracellularly by the secretory cmponent and J-chain.
12
13. 3.(IMMUNOGLOBULIN M ) IgM:--
IgM is an antibody. It is the largest of the immunoglobulins . It is the often
referred as the macroglobulin because of its high molecular weight (950,000). It is Y
Shaped.
They are polymers of usually 5 molecules (pentamers) each with polypeptide
chains.
A monomeric form (7s) of IgM is found on the B lymphocytes.
The serum level of IgM is very low, about 5 to 2 mg/ml.
13
14. 4.(IMMUNOGLOBULIN D ) IgD :--
IgD has a typical immunoglobulin structure with two light chain kappa or lamda
type. The two heavy chain are of delta type.
IgG is slightly larger than IgG, having a molecular weight of 180,000.
IgD is limited to the blood serum .The average serum level is 0.03 mg/ml.
It is found associated with the surface of B-lymphocytes.
14
15. 5.(IMMUNOGLOBULIN E ) IgE:-
1.Immunoglobulin E is a monomer having a
typical immunoglobulin structure. It has 2 light chains and 2 having chains.
The light chain is kappa or lemda type. The heavy chain is of epsilon.
The very low level of IgE is only because it is synthesised by a very few
plasma cells in the body .
It ‘s carbohydrate content is about 12%.
15
17. Isotypic Determinants -These determinants
distinguish the C regions of the H chain classes (and subclasses) and
the L chain types. Each isotype is encoded by a distinct gene that is
characteristic for a particular mammalian species and is present in all
members of that species. Antibodies to these determinants may be
raised by injecting IgG from one species into another species. Each Ig
class (IgG. IgM, IgA, IgD, and IgE) possesses a specific isotypic
determinant on its H chain.
Allotypic Determinants -The second group of
antigenic determinants are those found on the Igs of some, but not all,
members of a particular species. Allotypic determinants reflect genetic
polymorphism of Igs within one species. Antibodies can be formed
against an allotypic determinant by injecting Igs into another member
of the species that does not possess the alloantigen. In human the Gm
antigens are a good exam
Idiotypic Determinants -The third group of
antigenic determinants of Igs exist as a result of unique structures
generated by the hypervariable subregions (or complementarity-
determinging regions CDR) on L and H chains. The antigenic
determinants are called idiotopes, analogous to epitopes of classical
antigens.
Alpha-idiotopes lie outside the Ag-binding site.
Beta-idiotopes are close to the antigen binding site.
Gamma-idiotopes are formed by the antigen binding site.
17
18. PROPERTIES OF IMMUNOGLOBULINS:--
1.) Immunoglob0ulins are glycoproteins.
2.) They have a shape of Y and T.
3.) The molecular weight ranges from 150000 to 950000.
4.) Typically an immunoglobulin molecule is made up of four polypeptide chains of
which 2 are light chains and the remaining 2 are heavy chains.
5.) They cross placenta (IgG).
6.) They have reaginic activity (IgE)
7.) They are involved in complement fixation (IgG & IgM).
8.) They fix macrophages (IgG).
9.) They fix mast and basophil cells(IgE).
10.) They agglutinant antigens.
18
19. FUNCTIONS OF IMMUNOGLOBULINS:-
The main function of the immunoglobulin is protection of the body against. the
Invading micro-organism. The protective role is carried out in the following ways:
1. Agglutination of antigens .
2. Precipitation of antigens .
3. Lysis of the antigens .
4. opsonisation .
5. Tissue fixation.
6. Phagocytosis.
7. Chemotaxis.
8. Activation of mast cells and basophils.
19
20. CONCLUSION:-
When an immunoglobin reacts with an antigen,it is called an antibody.
When it does not react with an antigen it is simply called
immunoglobins.
All antibodies are immunoglobins; but all immunoglobins may not be
antibodies.
REFRENCE:-- K.C. SONY
20