The document describes the key parts and functions of a compound microscope used to examine peripheral blood smears. It outlines the proper procedure for making blood films, including using a spreader slide to create a thin, even smear. An ideal blood smear is translucent and uniformly thick. The process of fixing blood films with Leishman's stain and identifying features of a well-stained smear is also detailed. The major blood cell types—red blood cells, white blood cells including granulocytes and agranulocytes—are defined based on their appearance under different microscope magnifications.
I have listed out the LE cells structure and Microscopical examinaton of LE CELLS, Difference between tart cells and le cells, clinical symptoms and diagnostic procedure.
I have listed out the LE cells structure and Microscopical examinaton of LE CELLS, Difference between tart cells and le cells, clinical symptoms and diagnostic procedure.
An absolute eosinophil count is a blood test that measures the number of one type of white blood cells called eosinophils.
Eosinophils become active when you have certain allergic diseases, infections, and other medical conditions.
A brief presentation for second-year students in Iraqi Technical Institutes (studying Medical Laboratory Technology). This introduction covers also the teaching laboratories.
An absolute eosinophil count is a blood test that measures the number of one type of white blood cells called eosinophils.
Eosinophils become active when you have certain allergic diseases, infections, and other medical conditions.
A brief presentation for second-year students in Iraqi Technical Institutes (studying Medical Laboratory Technology). This introduction covers also the teaching laboratories.
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Hijama (Arabic: حجامة lit. "sucking") is the Arabic term for wet cupping, where blood is drawn by vacuum from a small skin incision for therapeutic purposes.The practice has Greek and Persian origin and is mentioned by Hippocrates.
Knee anatomy and clinical tests 2024.pdfvimalpl1234
This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
3. Parts of the Compound
Microscope
Tube
It is cylindrical tube through which the light from an
illuminated object traverses enroute its formation of an
image. It consists of an outer body tube and an inner draw
tube which can slide inside the outer, eye piece and lower
part carrying a revolving nose piece
Eye Piece
It comprises two co-axial lenses fixed to the ends of a
cylindrical tube, which can slide into the upper open end of
the draw tube. It is usually available in magnifications of
12.5X and 10X.
Nose Piece
It is a plate pivoted to the lower end of the tube. It is fitted
with objectives, any of which can at a time be brought in
line with the optical axis by rotating the nose piece. The
objectives are so fixed that the magnification will increase
as the user turns the revolving nose piece clockwise.
3
4. Objectives
These are cylindrical in shape and fitted with coaxial lenses. Three objectives are
provided:
(a) Low power objective (magnification 10X)
(b) High power objective (magnification 40X)
(c) Oil immersion objective (magnification 100X)
Adjustment Knobs
for coarse and fine adjustment.
Mirror
it is plane on one side and curved on other. The concave side is used to reflect light
from a point source while the plane side is used in diffuse light
Iris Diaphragm
It controls the amount of light reaching the condenser.
Condenser
It is system of lenses which focuses light from the source on the object. It is raised fully
while using the oil immersion lens and lowered fully while using the low power
objective.
Arm
4
6. PREPARATION OF BLOOD FILMS
Blood films can be made from anticoagulated, or finger-prick
blood.
PROCEDURES
Take 3 or 4 clean glass slides,the surface of which should be even and
smooth and place it on your work-table,.
Prick your finger and allow a medium-sized drop of blood to form on
the finger-tip.
Lift a slide from the table, holding it along its long edges. Then touch its
center, about 1 cm from the narrow end, to the blood drop.
Now grasp the long edges of a second slide, the “spreader”, between
thumb and fingers of your right hand,.
Place the narrow edge of the spreader on the first slide, at an angle of
40°, just in front of the blood drop. Pull the spreader back gently so that
it touches the front of the blood drop. Hold it there, (or move it a little
from side to side) till the blood, moving along the junction of the two
thus distributing the blood evenly across its width.
6
7. Steady the first slide with your left hand, and maintaining a
light but even pressure and 40° angle ,move the spreader
forwards to the left in a single, smooth, fairly fast gliding
motion, pulling the blood behind it in the form of a thin smear.
Make as many trials as possible to get acceptable films,
keeping in mind the features of an ideal blood smear, as
described below.
Dry the film by waving the slide in the air (Do not try to blot-dry
the film).
7
9. Features of an Ideal Blood Film
The blood film should occupy the middle two-thirds
(about 5 cm) of the slide, with a clear margin of about 2
mm on either side.
It should be tongue-shaped, i.e. broad at the head
(starting point), and taper towards the other end, but
without any ‘tails’.
It should be translucent, uniformly thick throughout, with
no vacant areas, striations (longitudinal or transverse), or
‘granular’ areas.
It should be neither very thick nor very thin (this can be
learned only with practice). A thin film looks faintly pink
against a white surface, while
9
10. Fixing and Staining of Blood
Films
Fixation is the process that makes the blood film and its cells
adhere to the glass slide. It also preserves the shape and
chemistry of blood cells as near living cells as possible.
Staining is the process that stains (colors) the nuclei and
cytoplasm of the cells. Both these purposes are achieved by
the Leishman’s stain.
LEISHMAN STAIN
It contains a compound dye—eosinate of methylene-blue
dissolved in acetone-free methyl alcohol.
i. Eosin. It is an acidic dye (negatively charged) and stains
basic (positive) particles—granules of eosinophils, and RBCs
a pink color.
ii. Methylene-blue. It is a basic dye (positively charged) and
stains acidic (negatively charged) granules in the
cytoplasm, nuclei of leukocytes, especially the granules of
basophils, a blue-violet color.
10
11. iii. Acetone-free and water-free absolute methyl alcohol.
The methyl alcohol is a fixative and must be free from
acetone and water. It serves two functions:
a. It fixes the blood smear to the glass slide. The alcohol
precipitates the plasma proteins, which then act as a ‘glue’
which attaches (fixes) the blood cells to the slide so that
they are not washed away during staining.
b. The alcohol preserves the morphology and chemical
status of the cells.
The alcohol must be free from acetone because
acetone being a very strong lipid solvent, will, if present,
cause crenation, shrinkage, or even destruction of cell
membranes. This will make the identification of the cells
difficult. (If acetone is present, the stain deteriorates
quickly).
The alcohol must be free from water since the latter may
result in rouleaux formation and even hemolysis. The
water may even wash away the blood film from the slide.
11
12. Procedure of Fixing And
Staining
Pour Leishman’s stain on slide enough to cover the
smear. Leave it about for 2 minute.
Now add few drops of buffer solution. Mix the stain and
buffer slowly by gently blowing air intermittently. Now
leave for 10 minutes(in summers put it under moist
chamber).
Wash the slide with tap water gently till the film has a
pinkish tinge.
12
13. Assessment of Stained Blood
Smears
Take an assessment of all the blood films. Examine with
naked eye first, and then under low and high
magnifications.
Choose the best stained films for cell counting.
Make sure that you can identify all the cells with
certainty.
Ensure that you are examining the blood smear side of
the slide. Hold the slide in bright light and tilt it this way
and that to see if there are any reflections. The clean side
shows reflections, while the side which has the blood
smear appears dull and does not show any reflections.
(You may also scratch the margin of the smear with a
pin).
13
14. Features of a Well-stained
Blood Smear
Naked eye appearance.
The smear appears translucent and bluish-pink when
seen against a white surface, its thickness being uniform
throughout. (It is assumed that an ideal blood film has
been stained).
Under low magnifications (100 x).
The red cells appear as dots, uniformly spread-out in a
single layer. The WBCs cannot be differentiated.
Under high magnification (450 x or 400 x).
The red cells are stained dull orange-pink and show a
central pallor (due to biconcavity) which, if wide, may
give the appearance of rings. The WBCs, with their nuclei
deep blue-violet, lie unevenly here and there among the
red cells. The platelets occur in small groups.
14
16. Identification of Various Blood
Cells
1. The red cells. (RBC)
Stained orange-pink, the red cells appear as numerous,
evenly spread out, non-nucleated, biconcave discs of
uniform size of 7.2–7.8 μm. Normally, the centralpaleness
occupies the middle third of the cells but is wider in
anemias.
16
17. 2. The leukocytes.(WBC)
Five main types of WBCs are commonly seen in blood
films.
They are all larger than the red cells, nucleated, and
unevenly distributed here and there among the red cells.
They include 3 types of granulocytes (Neutrophil.
Easionophill & Basophill), and 2 types of agranulocytes
(monocytes and lymphocytes).
There are fewer and poorly-stained WBCs in the head
end and the extreme tail; and some of these may be
distorted.
There appear to be more monocytes in the tail end,
probably dragged there by the spreader because of
their larger size.
Population-wise, neutrophils are the most numerous
leukocytes, then come the lymphocytes, monocytes,
17