This document discusses systemic lupus erythematosus (SLE), a chronic inflammatory disease that can affect many parts of the body including the heart, lungs, skin, joints, blood vessels, and kidneys. SLE is characterized by a rash, fever, renal issues, and abnormalities in blood counts. The disease involves the immune system inappropriately attacking the body's own tissues. A key indicator is the presence of lupus erythematosus cells, which are white blood cells that have ingested pieces of altered nuclear material from other cells. SLE most commonly affects women of childbearing age and those with African ancestry genetics.

1. LE CELL PREPARATION AND SYSTEMIC LUPUS
ERYTHEMATOSUS
Dr. sandeep singh
Department of Pathology
N.S.C.G.M.C. Khandwa

2.  Systemic Lupus Erythematosus (SLE) is a chronic rheumatic
disease which affects joints, muscles and other parts of the
body
 Lupus involves inflammation related to the immune system's
response to kill foreign agents, virus, bacteria
 Systemic lupus erythematosus involves chronic inflammation
that can affect many parts of the body, including:
 Heart
 Lungs
 Skin
 Joints
 Blood-forming organs
 Kidneys
 nervous system

3. INTRODUCTION CONT’D
 SLE is a connective tissue disease that affects most
commonly women of child bearing age and is
characterized by:
 Skin rash
 Fever
 Renal
 Cardiac and vascular lesions
 Anemia
 Leukopenia
 Often thrombocytopenia

4. INTRODUCTION CONT’D
 In SLE, there is a factor in the serum (immunoglobulin of
the IgG, IgM or IgA class) causes:
 Depolymerization of the nuclear chromatin of polymorphonuclear
leukocytes (neutrophils)
 Phagocytosis of this depolymerized material by an
intact neutrophil or monocyte giving rise to the Lupus
erythematosus (LE) cell

6. INTRODUCTION CONT’D
Peripheral blood film
showing a lupus
erythematosus (LE) cell
that has formed
spontaneously

7. INTRODUCTION CONT’D
 The LE cell is usually a neutrophil polymorph
(occasionally a monocyte or eosinophil) that has
ingested the altered nucleus of another polymorph
 The bulk of the cell is occupied by a spherical,
homogeneous mass that stains purplish brown
 The lobes of the ingesting polymorph appear
wrapped around the ingested material
 Occasionally, a group of polymorphs will collect
around an altered nuclear material and will
appear as a rosette formation

8. WHO IS AT RISK?
 women have increased incidence of SLE about 8 to 10
times more often than men
 SLE often occurs between the ages of 18 to 45
 Lupus occurs more often in African Americans
 Hereditary predisposition for this disease