Aseptic technique : Aseptic technique of preparation, Types of aseptic technique, principle of aseptic technique

1. Presentation onPresentation on
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Aseptic techniqueAseptic technique
PRESENTED BYPRESENTED BY
Md. Hamidul KowsarMd. Hamidul Kowsar
Dept. of PharmacyDept. of Pharmacy
State University of BangladeshState University of Bangladesh

2. Aseptic techniqueAseptic technique
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Aseptic means a germ-free condition
Aseptic technique is a method used in laboratories or clinical
setting to prevent the transmission of unwanted organisms.
Aseptic technique is commonly used in:
Syringes
Needles
Vials
Ampules
surgery equipment

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Importance of Aseptic TechniqueImportance of Aseptic Technique
Parenteral administration bypasses the skin and gastrointestinal
tract, the bodies natural barriers to infection
Giving a patient a contaminated product can cause serious
adverse effects including DEATH
Parenteral medications account for >40% of all medications
administered in institutional practice
Aseptic techniqueAseptic technique

4. Contamination
any effect or action that has a negative impact on a product's
integrity making it unfit for use
Purpose of Aseptic Technique
to protect the patient from contamination by pathogenic
organisms during medical and nursing procedures
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Aseptic TechniqueAseptic Technique
sterility (e.g. microorganism contamination)
particulate matter (e.g. dust, glass or
precipitation)

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Sources of product contaminationSources of product contamination
People (most common)
Touch contamination
Generation of particulates from shedding cells or hair
Supply air
Heating Ventilation and Air Conditioning (HVAC)
Infiltration
Particles from adjacent spaces (e.g. anteroom)
Internal generation
Walls, floors, ceilings, packaging, equipment

6. *. One way to minimize contamination is to not perform
your techniques in drafty areas
*. Any materials that will contact your experiments should
be sterilized by flaming
*. Also you should always make sure that the surface that
you are working on has been disinfected, eliminating
another potential source of contamination.
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Minimizing/Eliminating ContaminationMinimizing/Eliminating Contamination

7. The Principles of Aseptic TechniqueThe Principles of Aseptic Technique
1. Only sterile items are used within the sterile field.
2. Gowns are considered sterile only from waist to shoulders level in
front and the sleeves.
3. Tables are sterile only at table level.
4. Unsterile persons avoid reaching over a sterile field; sterile persons
avoid leaning over an unsterile area. 6.
5. Edges of anything that encloses sterile contents are considered
unsterile. 7.
6. Sterile field is created as close as possible to time of use.
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8. Sterile areas are continuously kept in view.
9. Sterile persons keep well within the sterile area.
10. Sterile persons keep contact with sterile areas to a minimum.
11. Unsterile persons avoid sterile areas
12. Destruction of integrity of microbial barriers results in
contamination.
13. Microorganisms must be kept to an irreducible minimum
The Principles of Aseptic TechniqueThe Principles of Aseptic Technique

9. Types of Aseptic Technique:Types of Aseptic Technique:
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1. Sterile Technique
 A technique that aims to achieve total absence of
microorganisms
 A Sterile Technique is only ever achieved in an operating suite or
using a laminar air flow cabinetSterile Technique include:
a.electric or gas heat method
b.chemical treatments method
c.Radiation method
1. Sterile Technique
2. Surgical Aseptic Technique
3. Standard Aseptic Technique

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2. Surgical Aseptic Technique
A technique that utilises a Critical Aseptic Field which is treated
like a key part and also uses:
such as sterile gloves, sterile gowns, cap, mask
3. Standard Aseptic Technique
A technique that utilises a General Aseptic Field, Critical Micro
Aseptic Fields, hand hygiene, non-touch technique and non-sterile
gloves to achieve a safe level of asepsis

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The best advantage of aseptic techniques is that harmful
microbes are not introduced.
For example in the surgical field or determining what microbe has caused
what disease.
An disadvantage is the cost of
specialized clothing,
materials, and
sterilized instruments.
Advantage & disadvantageAdvantage & disadvantage