This document discusses sterilization and various sterilization methods. It defines sterilization as making something free from all microorganisms, including bacteria and spores. It then describes different terms used in sterilization like disinfection, antisepsis, and discusses physical sterilization methods like dry heat, moist heat and radiation. Chemical sterilization methods using agents like alcohol, aldehydes, dyes, halogens and phenols are also outlined. Finally, the document briefly discusses the mechanical sterilization method of passing solutions through filters to remove microorganisms.
Sterilization (or sterilisation) referring to any process that eliminates (removes) or kills (deactivates) all forms of life and other biological agents (such as prions, as well as viruses which some do not consider to be alive but are biological pathogens nonetheless), including transmissible agents (such as fungi, bacteria, viruses, prions, spore forms, unicellular eukaryotic organisms such as Plasmodium, etc.) present in a specified region, such as a surface, a volume of fluid, medication, or in a compound such as biological culture media
STERILISATION, PHYSICAL METHODS OF STERILISATION, METHODS OF STERILISATION, VARIOUS METHODS OF STERILISATION, AUTOCLAVES, HOT AIR OVEN, DRY HEAT STERILISATION, MOIST HEAT STERILISATION
Sterilization (or sterilisation) referring to any process that eliminates (removes) or kills (deactivates) all forms of life and other biological agents (such as prions, as well as viruses which some do not consider to be alive but are biological pathogens nonetheless), including transmissible agents (such as fungi, bacteria, viruses, prions, spore forms, unicellular eukaryotic organisms such as Plasmodium, etc.) present in a specified region, such as a surface, a volume of fluid, medication, or in a compound such as biological culture media
STERILISATION, PHYSICAL METHODS OF STERILISATION, METHODS OF STERILISATION, VARIOUS METHODS OF STERILISATION, AUTOCLAVES, HOT AIR OVEN, DRY HEAT STERILISATION, MOIST HEAT STERILISATION
The above PPT includes different methods of sterilization- Dry heat, Moist heat, Radiation and Chemical methods. It also includes the basic knowledge on sterilization and tests for sterility.
Autoclave, types of autoclave, horizontal autoclave, vertical autoclave, vacuum type autoclave, pressure cooker type autoclave. their purpose, precaution, etc....
The above PPT includes different methods of sterilization- Dry heat, Moist heat, Radiation and Chemical methods. It also includes principle and working of hot air oven and autoclave.
The above PPT includes different methods of sterilization- Dry heat, Moist heat, Radiation and Chemical methods. It also includes the basic knowledge on sterilization and tests for sterility.
Autoclave, types of autoclave, horizontal autoclave, vertical autoclave, vacuum type autoclave, pressure cooker type autoclave. their purpose, precaution, etc....
The above PPT includes different methods of sterilization- Dry heat, Moist heat, Radiation and Chemical methods. It also includes principle and working of hot air oven and autoclave.
Control of microrganisms - Food Microbiology - Food Processing MUTHUGANESAN N
1. Sterilization - the destruction of all microorganisms, including endospores, on an object or in a material.
2. Disinfection - the destruction of pathogens, but not endospores, on an object or in a material. The number of pathogens is reduced or growth is inhibited to a level that does not produce disease.
3. Antisepsis - chemical disinfection of the skin, mucosal membranes, or other living tissues.
4. Germicide ("cide" = kill) - a chemical agent that rapidly kills microorganisms.
Specific germicides include:
(I) Sporicide - kills spores
(II) Bactericide - kills bacteria
(III) Viricide - kills viruses
(IV) Fungicide - kills fungi
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
2. STERILIZATION
Sterilization is making a substance free from all micro
organisms both in vegetative and sporing states.
Spore is a reproductive structure that is adapted for dispersal
and surviving for extended periods of time in unfavourable
conditions.
Spores form part of the lifecycles of many bacteria, plants,
algae , fungi and some protozoa.
3. Terms usedin sterilization:
Disinfection:
The destruction or removal of all pathogenic organisms capable of giving
rise to infection.
Disinfection does not affect spore state organisms.
Antisepsis:
The term is used to indicate the prevention of infection, usually by
inhibiting the growth of bacteria in wounds or tissues.
This is done by the antiseptics
Chemicals or disinfectants which can be safely applied on skin or mucous
membrane to prevent infection by inhibiting the growth of bacteria.
Bactericidal agents / germicides:
Those which able to kill bacteria.
4. Bacteriostatic agents:
Only prevent multiplication of bacteria, but they remain
alive.
Cleaning:
Important preparatory step before sterilization or
disinfection, by removing soil and other dirt.
Decontamination:
The process of rendering an article or area free of
contaminants, including microbial, chemical, radioactive and
other hazards.
5. • Dry heat sterilization
• Moist heat sterilization
• Sterilization by radiation
Physical
method
• Gaseous sterilization
• Sterilization by disinfectant
Chemical
method
• Pass through bacteria-proof filter
Mechanical
method
METHODS/AGENTSOF
STERILIZATION
6. DRY HEAT STERILIZATION
Instrument- ‘OVEN’
OVEN : specially designed instrument - electrically heated and thermostatically
controlled. Expose at 160 ºC for 1 hour.
Advantages :
1. it is suitable method for sterilization of substances destroyed by moisture.
2. They do not require water and there is not much pressure build up within the
oven, unlike an autoclave, making them safer to work with.
3. Suitable to be use in a laboratory environment.They are much smaller than
autoclaves but can still be as effective.
Disadvantage:
As they use dry heat instead of moist heat, some organisms like prions, may not be
killed by them every time.
7.
8. MOIST HEAT STERILIZATION
Instrument- ‘AUTOCLAVE’
Heating process in autoclave - saturated steam under
pressure is allowed to penetrated through materials for 15
minutes and temperature 121º c.
Advantage:
micro organism are killed most efficiency in lesser time due
to high pressured saturated steam
Disadvantage:
unsuitable for materials not withstanding temperature of
115ºC or more during heating
9.
10. STERiLIZATION BY RADIATION
Two techniques involved:
Alteration of chemicals lead to form new compound in cells
destroying the micro-organism itself
Vital structure like nuclear protein are destroyed killing the micro-
organism.
e.g., Co-60 - used for gamma ray sterilization process.
2 types of radiation:
Ionising radiation
Non-ionising radiation
11. Ionising radiation Gamma rays:
X-rays: Used for sterilising plastics, syringes,
Gamma rays –
generally obtained from radio isotope(Co-60) during disintegration of unstable
atoms
kill micro-organisms by isolating atoms of essential substance of cells present in
them
ADVANTAGE :
1. No significance rise in temperature
2. Continuous process due to short exposure time.
DISADVANTAGE :
1.May lead to color change.
2. Solubility of preparation leading to decomposition of certain materials.
12. Non-ionising radiation
Infrared-Used for rapid mass sterilization of prepacked items
such as Syringe,Cathaters
UV : Used for disinfecting enclosed area such as entryways,
operation theatres and labs.
Testing - Efficacy of Radiation:
Radiation control indicator disc: Special paper sticker which is
yellow in colour, is sticked on the articles. After proper sterilization
disc colour changes to red.
13. Chemical methods of Sterilization
Chemical agents:
Action of chemical agents:
i. Protein coagulation
ii. Disruption of cell membrane resulting in exposure,
damage/loss of contents
iii. Removal of sulfhydryl group essential for normal
functioning of enzyme
iv. Substrate competition.
14. Commonly used chemical :
Alcohol
a. Ethyl alcohol ,Isopropyl alcohol
b. These must be used at concentration 60-90%.
c. Isopropyl alcohol used in disinfection of clinical thermometer.
d. Methyl alcohol is effective against fungal spores, treating cabinets and
incubators.
e. Methyl alcohol is also toxic and inflammable.
Aldehyde
I. Formaldehyde:
Having Bactericidal, sporicidal and has lethal effect on viruses.
Used to preserve anatomical specimens, destroying anthrax spores on hair
and wool.
15. II .Glutaldehyde:
Effective against tubercle bacilli, fungi, viruses.
Less toxic and irritant to eyes, skin
Used to treat anaesthetic rubber, face masks, plastic endotracheal
tubes, metal instruments and polythene tubing.
Dyes:
2 groups of dyes:
1.Aniline dye 2.Acridine dye
Both are bacteriostatic in high dilution but are of low bactericidal
activity.
Aniline dye is more active against gram +ve than gram-ve
organisms.
16. Some important dyes:
Proflavine Acriflavine Euflavine Aminacrine
These Impair the DNA complexes of the organisms and thus kill
or destroy the reproductive capacity of the cell.
Halogens
Iodine :
Used as Skin disinfectant
Having Active bactericidal activity &moderate action on spores.
Chlorine :
Used to disinfect Water supplies, swimming pools and food and
dairy industries.
Along with hypochlorides are bactericidal. Also act on viruses.
17. Phenols :
These are obtained from distillation of coal tar between 170-270
C.
Lethal effects are:
a. Capacity to cause cell membrane damage, releasing cell
contents and causing lysis.
b. Low concentration will precipitate proteins.
18. Gases:
Types of gases used for sterilization:
i. Ethylene oxide
ii. Formaldehyde gas
iii. Beta propiolactone (BPL).
19. Ethylene oxide:
The gas used is safe & non-inflammable.
Now-a-days, ethylene oxide most widely used gaseous sterilization
agent in medical science.
Action is due to its alkylating the amino, carboxyl, hydroxyl and
sulphydryl groups in protein molecules.
Also on DNA and RNA.
Items: heart-lung machines, respirators, sutures, dental equipment,
books, clothing.
Advantages: It has penetration power quite useful for sterilizing
surgical instruments (such as catheter, needles, plastics, disposables)
Disadvantages:
1. Very slow sterilization process
2. Very costly equipment
20. Formaldehyde gas:
This is widely employed for fumigation of OT and other rooms.
Formaldehyde is produced by adding 150g of KMnO4 to 280ml of
formalin for every 1000cu.ft of room volume, after closing the
windows and other outlets.
After fumigation, the doors should be sealed and left unopened for
48 hours.
Betapropiolactone:
Product of ketone and formaldehyde with a boiling point of 163 C.
Having rapid bactericidal activity but carcinogenic.
Capable of killing all microorganisms and is very active against
viruses.
21. DISINFECTION
Decontamination - removal of microorganisms
contaminating an object
Preservation - preventing methods of microbes-caused
spoilage of susceptible products(pharmaceuticals, foods)
Sanitization - removal of microbes that pose a threat to
the public health, food industry, water conditioning
sanitizer-an agent, usually a detergent, that reduces the
numbers of bacteria to a safe level
22. MECHANICAL METHOD
The solution to be sterilized is passed through depth filter or
screen-filter which includes:
I. Particulate filters
II. Microbial filters
III. Final filter
Pharmaceutical solutions are sterilized by this method.
The micro-organism are physically removed by absorption on the
filter medium or by mechanism.
Sterilization test must be done.