Learn Tau in 3 minutes | Microtubule-associated protein tau (MAPT)
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Does your next experiment involve Tau (microtubule-associated protein tau, MAPT)? This is a presentation about tau, intended for scientists who are designing controls and performing immunoassays detecting tau. It contains information about western blot band size, protein tissue expression, and interesting facts. Anti-Tau/MAPT Antibody Picoband™ (A00097-3) https://www.bosterbio.com/anti-tau-mapt-picoband-trade-antibody-a00097-3-boster.html References: Uniprot, Proteinatlas.org Learn more about Tau (infographic and discussion): https://www.bosterbio.com/bosterbio-gene-info-cards/MAPT Boster Biological Technology Website: www.bosterbio.com Email: support@bosterbio.com
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Learn Tau in 3 minutes | Microtubule-associated protein tau (MAPT)
- 1. www.bosterbio.com TAU IN 3 MINUTES Quick facts, control designs, Western Blot MW. Info you can use to find the best TAU antibody.
- 2. FACTS 01. Function, structure, etc. TISSUES 02. Expressing tissues/cells WESTERN BLOT 03. Expected band sizes HISTORY 04. …and medical significance TABLE OF CONTENTS
- 3. 3 ● Microtubule-associated protein tau (Tau) ● Single chain protein ● Isoforms ○ Isoform PNS-tau (Big tau) is 758 AAs long ○ 6 isoforms between 352–441 AAs long ● In WB, tau is observed to be several bands spanning from 50 kDa to 80 kDa 01. FACTS AlphaFold predicted structure Image source: https://alphafold.ebi.ac.uk/entry/P10636
- 4. 4 02. TISSUES Image source: https://www.proteinatlas.org/ENSG00000186868-MAPT/tissue
- 5. 5 03. TAU WESTERN BLOT After reviewing the selected images, we observe Tau shows in WB as a collection of bands from 50 kDa to 80 kDa. PMC5675734 Figure 1 PMC4294473 Figure 10 PMC5895994 Figure 2 Boster Bio Tau Antibody (A00097-3) WB image 2861 images available on PMC: https://www.ncbi.nlm.nih.gov/pmc/?term=tau+western+blot&report=imagesdocsum
- 6. 6 Marc Kirschner identified the first microtubule-stabilizing protein tau in 1975 at Princeton. 04. HISTORY Tau aggregation is observed in tauopathies and other neurodegenerative diseases, such as Alzheimer’s disease. 1975 CLINICAL POTENTIAL
- 7. 3942 B Valley Ave Pleasanton, CA 94566 Tel: (888) 466-3604 Email: support@bosterbio.com www.bosterbio.com
Editor's Notes
- Does your next experiment involve Tau? This video is for scientists who want to run Western blot, IHC, flow or other immunoassays against tau.
- We will cover some basic facts, protein expression, and Tau’s expected behavior in Western blot. All information in this video is based on public information and no proprietary experimental evidence was used.
- Topic 1: Quick facts about tau. Microtubule-associated protein tau is a highly soluble protein. Although Uniprot reports 9 isoforms produced by alternative splicing, 6 isoforms with a range of 352 to 441 amino acids are more commonly discussed. Isoform PNS-tau, a larger isoform of tau also known as Big tau, is 758 amino acids long. In general, tau proteins run about 50 kilodaltons to 80 kilodaltons on Western blot. Hyperphosphorylated tau is observed in Alzheimer’s disease, accompanying the tangles that progressively form in the brain. Tau is one of the most researched proteins of interest in Alzheimer’s disease and other dementias.
- Topic 2: Where is tau expressed? High levels of tau have been found in neurons. Isoform PNS-tau is primarily expressed in the peripheral nervous system while other isoforms are expressed in the central nervous system. According to Protein Atlas, tau is also expressed in breast tissue and the kidney, more specifically the renal tubules. Lower levels of expression are found in the salivary gland, testes, pancreas, adipose tissue, etc. For more information, visit protein atlas dot org and check out which cell lines express tau. This information may be useful for designing positive and negative controls for western blot and immunohistochemistry.
- Topic 3: Tau in Western blotting. Antibody specificity can make or break an experiment. The last thing we want is to have our papers retracted due to a false positive figure. After looking through Western blot images from publications and antibody companies, we can see that several bands can be expected in a tau western blot, spanning from 50 kilodaltons to 80 kilodaltons. Check out the PMC link for more images, which is also provided in the description box.
- Topic 4: Some interesting facts about tau. Marc Kirschner identified the first microtubule-stabilizing protein tau in 1975 at Princeton. Tau is observed to be present in several forms of dementia and neurodegenerative disorders. In healthy individuals, Tau is a highly soluble protein. However, in Alzheimer’s disease, hyperphosphorylated Tau is associated with insoluble neurofibrillary tangles that build up in the brain. Because of this, Tau’s involvement in the mechanism of action of dementia is of high interest to pathologists and some pharmaceutical companies.