BRCA1 is a tumor suppressor protein involved in DNA repair. It is 1,863 amino acids long and has a molecular weight of around 207 kDa. BRCA1 is expressed in breast, ovarian, and prostate tissues and cells. Western blots typically show the BRCA1 protein band at 207-220 kDa. The BRCA1 gene was discovered almost 30 years ago and helps repair DNA, while mutations increase cancer risk.
5. 03. BRCA1 WESTERN BLOT
1073 images available on PMC:
https://www.ncbi.nlm.nih.gov/pmc/?term=brca1+western+blot&report=imagesdocsum
After reviewing several WB images, we observe BRCA1 at ~ 207-220 kDa.
PMC7265328 Figure 4
PMC2747554 Figure 4
PMC773359 Figure 6
Boster Bio BRCA1
Antibody (Rabbit
Polyclonal) Western
blot image
PMC4465499Figure 6
6. The BRCA1 gene was discovered
almost 30 years ago.
04. HISTORY
BRCA1 helps repair DNA double-
strand breaks to protect our
genome. Heritable BRCA1
mutations dramatically increase
breast and ovarian cancer risk.
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Editor's Notes
Does your next experiment involve BRCA1? This video is for scientists who want to run Western blot, IHC, flow or other immunoassays against BRCA1.
We will cover some basic facts, protein expression, and BRCA1’s expected behavior in Western blot. All information in this video is based on public information and no proprietary experimental evidence was used.
Topic 1: Quick facts about BRCA1. Breast cancer type 1 susceptibility protein, or BRCA1, is an E3 ubiquitin protein ligase. As the name suggests, mutations to BRCA1 increase the risk for breast or ovarian cancer. The BRCA1 protein is highly involved in DNA repair and is known as a tumor suppressor. BRCA1 is a large protein of 1863 amino acids and its calculated molecular weight is about 207 kilodaltons. According to Uniprot, eight isoforms have been reported.
Topic 2: Where is BRCA1 expressed? Due to BRCA1’s important role in DNA repair, BRCA1 is well expressed in multiple tissues, such as the lymph nodes, skin, brain, pancreas, kidney, endocrine tissues, liver, gallbladder, and other tissues. You can find more information on protein atlas dot org to check out which cell lines express BRCA1 and which ones don’t. This information will come in handy when designing positive and negative controls for western blot and immunohistochemistry.
Topic 3: BRCA1 in Western blotting. Antibody specificity can make or break an experiment. After looking through Western blot images from publications and antibody companies, we can see that one band can generally be expected in a BRCA1 Western blot at around 207 to 220 kilodaltons. Check out the PMC link for more images, which is also provided in the description box.
Topic 4: Some interesting facts about BRCA1. The landmark discovery of the BRCA1 gene in 1994 led to a greater understanding of breast and ovarian cancer. The tumor suppressor BRCA1 specifically functions by helping to repair double-strand breaks in DNA, which helps to protect our genome. Heritable mutations of BRCA1 led to a dramatic increase in the risk for developing breast or ovarian cancer. Further research on BRCA1 and its role in cancer can potentially lead to greater therapeutic benefits in cancer patients.