Learn ESM1 in 3 minutes | Endothelial cell-specific molecule 1, Endocan
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This document provides key information about the endothelial cell-specific molecule 1 (ESM1) for selecting the best ESM1 antibody. ESM1 is a ~25 kDa protein expressed in endothelial cells and tissues involved in angiogenesis. Western blots of ESM1 show a band around 20-25 kDa. ESM1 promotes angiogenesis and tumor progression, making it a potential cancer biomarker.
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Learn ESM1 in 3 minutes | Endothelial cell-specific molecule 1, Endocan
- 1. www.bosterbio.com ESM1 IN 3 MINUTES Quick facts, control designs, Western Blot MW. Info you can use to find the best ESM1 antibody.
- 2. FACTS 01. Function, structure, etc. TISSUES 02. Expressing tissues/cells WESTERN BLOT 03. Expected band sizes SIGNIFICANCE 04. Clinical potential TABLE OF CONTENTS
- 3. ● Endothelial cell-specific molecule 1 (ESM1), aka Endocan ● Total chain length is 184 amino acids ○ Signal peptide (19 amino acids) is cleaved to form the active protein (165 amino acids) ● Active form: ~25 kDa 01. FACTS AlphaFold predicted structure Image source: https://alphafold.ebi.ac.uk/entry/Q9NQ30
- 4. 02. TISSUES Image source: https://www.proteinatlas.org/ENSG00000164283-ESM1/tissue
- 5. 03. ESM1 WESTERN BLOT 19 images available on PMC: https://www.ncbi.nlm.nih.gov/pmc?term=esm1%20western%20blot&report =imagesdocsum After reviewing several WB images, we observe ESM1 at ~20-25 kDa. PMC8105853 Figure 2 Boster Bio ESM1 Antibody (A04169-1, Rabbit Polyclonal) PMC6392130 Figure 3 PMC5710926 Figure 5
- 6. ESM1 is involved in angiogenesis and promotes angiogenic sprouting. 04. HISTORY Overexpression of ESM1 is observed in human tumors and tumor progression, indicating its potential as a cancer biomarker. ANGIOGENESIS ROLE IN TUMOR
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Editor's Notes
- Does your next experiment involve Endocan? This video is for scientists who want to run Western blot, IHC, flow or other immunoassays against ESM1.
- We will cover some basic facts, protein expression, and ESM1’s expected behavior in Western blot. All information in this video is based on public information and no proprietary experimental evidence was used.
- Topic 1: Quick facts about Endocan. Endothelial cell-specific molecule 1 or ESM1 is a soluble proteoglycan, which is expressed by the vascular endothelium. It is a key player in the regulation of major processes such as cell adhesion, inflammatory disorders, and tumor progression. The total chain length is 184 amino acids, out of which the signal peptide of 19 amino acids is cleaved to form the active protein of 165 amino acids. The active protein runs about 25 kilodaltons on Western blot.
- Topic 2: Where is endocan expressed? High levels of ESM1 RNA have been found in the thyroid gland, lung, parathyroid gland, and adrenal gland with the highest levels detected in the kidney. They are also expressed at lower levels in the spleen, bone marrow, amygdala, cerebellum, and thymus. You can find more information at protein atlas dot org including which cell lines express endocan and which ones don’t. This information will come in handy when designing positive and negative controls for western blot and immunohistochemistry.
- Topic 3: Endocan in Western blotting. Antibody specificity can make or break an experiment. After looking through Western blot images from publications and antibody companies, we can see that one band can be expected in an ESM1 Western blot at around 20 to 25 kilodaltons. Check out the PMC link for more images, which is also provided in the description box.
- Topic 4: Some interesting facts about Endocan. ESM1 is an important protein involved with angiogenesis, inflammation, and tumor progression. Research has shown that it is overexpressed in human tumors, with elevated serum levels being observed in late-stage lung cancer patients. Since the overexpression of endocan aids in tumor progression, ESM1 could be a biomarker for both inflammatory disorders and tumor progression, potentially serving as a therapeutic target in cancer.