X-ray crystallography is used to determine the structure of molecules like proteins and DNA by fixing samples into crystals and bombarding them with X-rays. The X-rays cause diffraction patterns based on the molecular structure, with different angles of diffraction indicating different planes of the crystal structure. Analysis of the diffraction data reveals information about electron density and molecular structure that allows researchers to identify the positions of atoms within the sample.

1. X-Ray Crystallography
Contact: biokam25@gmail

2. Moving molecule structure identification is tough so we need to fix them by using Crystallization
Moving molecules
In moving molecules capterization is
Tedious
Fixed Sample: Defined Capture

3. X-Ray on Crystals
X-Ray waves Crystals
+
DNA
 X-Rays on Crystal (carries Protein or DNA sample) causes diffraction, angle diffraction determines the
structure.
 Based on the samples the diffraction may varies (consider the each side of cubes as a plane), X- Rays on the
planes shows some reflection that detected by the detector.
 Data of diffraction help us to identify the molecular structure.
 Reflection and diffraction are same.

4.  Angle of Diffraction
 Intensity of the spot directly proportional to
the electron present
 High density of el- directly proportional to the
molecule occupy

5. Many Thanks