Farmers in Bihar, India have doubled their rapeseed (mustard) yields using Sustainable Rapeseed Intensification (SMI) methods inspired by the System of Rice Intensification (SRI). SMI involves low seed rates, seed priming, transplanting young seedlings in squares with wide spacing, mechanical weeding, and increased irrigation. Yields of 2.5 to 3 tons per hectare have been reported, compared to 1 ton with conventional methods. SMI can increase incomes for farmers while reducing chemical use and sustaining soil quality.
BERSEEM Trifolium alexandriannium is an annual leguminous fodder crop.
One of the most suitable fodder crops for areas below 1700 m altitude with irrigation facilities.
Remains soft and succulent at all stages of growth.
It can be grown without irrigation in areas with high water table and under water-logged conditions.
Indigenous to Egypt.
Production technology of onion and garlicRakesh Rajput
Production technology of bulb crop.
Production technology of Onion and garlic.
Cool season vegetables.
Cultivation of onion and garlic.
Diseases of onion and garlic
Production technology of onion ppt
It is helpful for chickpea cultivation & production in Agricultural sector.These presentation include all the information up to storing. its language is very simple that why everybody easy to understand.
..........Thanks
Training is an important operation in grapes.
It helps to maintain the stature and spread of the vine and facilitates operations like pruning, intercultivation, spraying and harvesting.
Many training systems are in vogue in India, but the most popular are Bower, Telephone and Kniffin systems.
BERSEEM Trifolium alexandriannium is an annual leguminous fodder crop.
One of the most suitable fodder crops for areas below 1700 m altitude with irrigation facilities.
Remains soft and succulent at all stages of growth.
It can be grown without irrigation in areas with high water table and under water-logged conditions.
Indigenous to Egypt.
Production technology of onion and garlicRakesh Rajput
Production technology of bulb crop.
Production technology of Onion and garlic.
Cool season vegetables.
Cultivation of onion and garlic.
Diseases of onion and garlic
Production technology of onion ppt
It is helpful for chickpea cultivation & production in Agricultural sector.These presentation include all the information up to storing. its language is very simple that why everybody easy to understand.
..........Thanks
Training is an important operation in grapes.
It helps to maintain the stature and spread of the vine and facilitates operations like pruning, intercultivation, spraying and harvesting.
Many training systems are in vogue in India, but the most popular are Bower, Telephone and Kniffin systems.
Pulses are a very important source of protein in Indian diets as majority of population is vegetarian. however, the production of pulses is not keeping pace with the growing population in the country. lentil is one of the important Rabi pulses. it is one of the oldest pulse crops and the most nutritious of the pulses. it is also used as a cover crop to check the soil erosion in problem areas. lentil contributes about 6% in total pulses area as well as production of India. It is mostly eaten as "DAL". the pulse is first converted into split pulse or 'dal' by the removal of skin and the separation of the fleshy cotyledons. It is cooked easily and hence preferred. It is good for patients too. Lentil contains about 11% water, 25% protein and 60% carbohydrates. It is also rich in calcium, iron and niacin
Pulses are a very important source of protein in Indian diets as majority of population is vegetarian. however, the production of pulses is not keeping pace with the growing population in the country. lentil is one of the important Rabi pulses. it is one of the oldest pulse crops and the most nutritious of the pulses. it is also used as a cover crop to check the soil erosion in problem areas. lentil contributes about 6% in total pulses area as well as production of India. It is mostly eaten as "DAL". the pulse is first converted into split pulse or 'dal' by the removal of skin and the separation of the fleshy cotyledons. It is cooked easily and hence preferred. It is good for patients too. Lentil contains about 11% water, 25% protein and 60% carbohydrates. It is also rich in calcium, iron and niacin
Saguna Rice Technique is a unique new method of cultivation the rice and related rotation crops without ploughing, puddling and transplanting (rice) on permanent raised beds. This is a zero till, Conservation Agriculture (CA) type of cultivation method evolved at Saguna Baug, Neral, Dist. Raigad, Maharashtra, India
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
In this talk, I will discuss our work in (1) building an integrative knowledge infrastructure to prepare FAIR and "AI-ready" data and services along with (2) neurosymbolic AI methods to improve the quality of predictions and to generate plausible explanations. Attention is given to standards, platforms, and methods to wrangle knowledge into simple, but effective semantic and latent representations, and to make these available into standards-compliant and discoverable interfaces that can be used in model building, validation, and explanation. Our work, and those of others in the field, creates a baseline for building trustworthy and easy to deploy AI models in biomedicine.
Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
1. OBJECTIVE > DOUBLING FARMER’S
INCOME BY INCREASING YIELD
Presented to, Presented by,
EXT- 411 (RAWE)
2.
3. In 2009-10 ,the Agricultural Technology Management
agency
(ATMA) and Pradan initiated a programe to use SRI
method
in rapeseed cultivation .Initially, there were woman farmers
from Gaya district from Bihar. Who started the
experiment.
In 2011-12, the Sir Dorabji Tata Trust (SDTT) extended
support
for this innovation effort, so that about 1600 farmers were
able use SRI method for rapeseed this season
This manual have specific steps for cultivated rapeseed/
mustard with SRI method. It should be equally useful for
farmers and village extension workers. It is intended to help
INTRODUCTION
4. How is rapeseed cultivation with SRI methods?
The fallowing core principle of SRI for rice are applied in SMI
rapeseed cultivation :
Low seed rate: Only 50 to 250 grams per
acre
Priming of seed with seed selection and
treatment
Preparing nursery with treated/sprouted
seed Transplanting seedling when they are about 8 to12
days old and have only 3-4 leaves
Wide and uniform spacing of single plants across the field
Aerate the soil while suppressing the weeds using
mechanical rather than chemical means
5. Other practices in crop husbandry are similar to
normal methods
The yield achieved by applying SRI principles is
about double that achieved with conventional
methods.
farmers from Gaya and Nalanda district in Bihar
have reported yields of 2.5 to 3.0 tones per hac.
or more.
6. Seed varieties and seedling rates
Seed selection
seed used to always healthy ,improved resistance
disease and pest. No mixture of other seed
Seeding rate
The quantity of seeds to be used depends upon
the length of the crop cycle of the variety chosen.
If the variety is of longer duration, the quantity of
seeds required is less; whereas for short duration
varieties, the seed requirement is greater, as shown
below.
7. Duration of the
variety
Spacing(in grams) Seed rate(grams)
Less than 100 days 30* 30 250
100-020 days 45* 45 200
120-130 days 60* 60 125
130- 140 days 75* 75 75
8. Priming of seeds and seed
treatment Put the seeds into a ceramic or non-metallic pot with half a
liter of warm water having a temperature of about 60
celsius.
Stir the mixture so that any damaged or undeveloped
seeds
float on the surface. Remove these, keeping just the
heavier
seeds for use.
Pour into the pot a mixture of cow urine, jaggery (coarse
sugar),
and vermicompost, in roughly equal proportions. Leave the
to soak in the mixture for 6-8 hours.
The warm water should be about double the quantity of
seeds being prepared, while the other materials should
each be about half the quantity of the seeds. .Example, if the amount of seed is 250 grams, the lukewarm wat
should be about 500 ml, and each of the other items should be
about 125 grams.
9. After 6 to 8 hours, separate the seeds from the mixture and add
a small amount of Trichoderma , a beneficial microorganism tha
supports plant around them. The rate for adding Trichoderma
should be 4 grams per kg of seed, so 1 gram of this microorgani
additive would be sufficient for 250 grams of seeds. •
Keep the seeds this way for about 8 to 10 hours to let them
germinate. .
Once the seeds have germinated, plant the sprouted seeds in
the raised nursery beds, described next.
seed Warm water Jaggery Cow urine vermicompost
10. Nursery preparation
Prepare a raised seedbed in a field such as is generally
used for vegetable cultivation. The area of the seedbed
will depend upon the seed variety used; a smaller area
is enough for a longer-duration variety; a larger area is
needed for a shorter-duration variety.
Duration (in days) Area of the nursery (in
square meter)
Less than 100 days 60
110-120 50
120-130 30
130-150 20
Mix the soil of the seedbed with vermicompost
(2 to 2.5 kg per square meter) and carbofuran
(2 to 2.5 grams per square meter)
11. The bed should be 4 to 6 inches above the ground
level, and the width of the bed should be one meter
If there is more than one nursery bed, a channel
of one-foot width should be dug between the two beds.
The soil should be moist when putting in the sprouted
seeds, and the sprouted seeds should be at a depth
of one-half inch, keeping a spacing of about 2 X 2
inches between the sprouted seeds
Cover the seeds with vermicompost and spread a
mulching of paddy straw over the bed
Each morning and in the evening, spray or sprinkle
water on the nursery for gentle irrigation
Transplant the young plants between 8 to 12 days
12.
13. Field preparation
Plough the field beforehand 2-3 times, so that the soil is
well pulverized. If the soil moisture is insufficient, provide
some supplementary irrigation before tilling the land.
In addition, remove the weeds from the field
Mark the places in the field where the mustard seedlings
should be planted, using a spade or a rake or a hoe to
designate the proper spacing in a square grid pattern;
follow the spacing instructions above according to the variety
At each marked place, dig a small pit, 6 inches in diameter and
8-10 inches in depth. Leave the pits open, exposed to natural
sunlight for 2-3 days.
After 2-3 days, apply 300-400 grams of the mixture described
below in the pits, one by one.
14. Usually for an acre of mustard crop planting, start by mixing
8 tons of compost with 1.5 kg of Trichoderma , then mixing
this with soil collected from and near the pits, and putting
this mixture into the pits.
For an acre, use about 27 kg of di-ammonium phosphate
(DAP) and 13.5 kg of potash (MOP). Mix these into the soil
and compost and put the mixture into the dug pits, nearly
filling them with these materials. Soil should generally be
about 50% of what goes into the pit. Before transplanting,
each of the pits s hould be kept or made moist
15. Transplanting with SMI methods
Before transplanting, irrigate the nursery approximately 2
hours
in advance to moisten the soil for removing the plants Carefully uproot the seedlings, keeping the soil intact;
if possible use a trowel or spade that gives support
to the soil, so that it remains intact with the roots
Transfer the uprooted seedlings to the main plot in the
next 30 minutes, before the roots and soil can dry out
Transplant the seedlings at a shallow depth in the pits
Irrigate the pits manually with a sprinkler/sprayer for the
first 3-5 days. This helps in establishing the plants in the
main plot
16. Care of the field up to 30 days after transplanta
Irrigate the field with first irrigation on the 15th day after
transplantation. Apply a dose of urea (27 kg per acre)
on the 16th day when there is still moisture in the field.
It is better to apply urea close to the roots of the plants
On the 20th day, remove any weeds by hoeing in between
the rows. Mix about 0.4 ton of vermicompost (VC) and 3 kg
of phosphatesolubilizing bacteria (PSB) per acre into the soil
using a dry weeder or spade. The timing of this 1st weeding
and application can vary depending on local agro-climatic
conditions
Irrigate the field for a second time on the 30th day, and do
a second round of weeding and hoeing. During hoeing,
mix 13.5 kg per acre of Biozyme (a biofertilizer) into the
soil for further enrichment of the soil biota
17.
18. Care of the field 35-50 days after transplantatio
The plants begin growing faster from 30 days after transplantat
Therefore, the plants need more moisture and nutrition. Hence,
on or about the morning of the 35th day, apply a dose of urea
(13.5 kg per acre) near the roots of the plants and irrigate the
field for the third time
About 3-5 days after the third irrigation, or about 40 days
after transplanting, along with a third weeding/hoeing,
apply 13.5 kg of potash (MOP) to the soil. Use a weeder
or spade to break up the soil and then to earth up some
soil around the base of the plants, as is usefully done with
potatoes, i.e., up to one foot. This promotes more root growth
19.
20. Care of the field from 50 days after
transplantation The plants grow very fast beyond 50 days after transplanting.
The stems, branches and leaves are growing and thickening
at this time
Aphids should be managed effectively at this time. At the start
of the reproductive stage, the plants begin flowering, and
subsequently, the siliqua (pod) formation and grain filling take
place. In this period, the crop is most susceptible to pest attack
Therefore, care and attention are required all the time, keeping
an eye out for such problems
For proper growth and grain filling, irrigate the plot on
60th, 80th, 100th and 120th days after transplanting.
Irrigation timing depends on the agro-climatic conditions.
If irrigation is not provided on or about these days, there
will be drastic reduction in the yield of the crop
21.
22. Differences between SMI and conventional method
for mustard/rapeseed cultivation (in acre)
Description Traditional method SMI method
Seed rate 5.5 kilograms 200-300 grams (>90 %
reduction)
seed treatment Not done With jaggery, cow urine,
warm water, and
vermicompost
Planting method broadcasting transplanting
Plant spacing Irregular From 30*30cm up to
75*45cm
Weeding Not done On the 15,25,$25 days
after plantig
Irrigation 2 to 4 times 5 to 6 times
Branch per plant 3 to 5 8 to 15
Yield per acre 5 to 6 q 12 to 15q
seed wt. per plant 15 to 30 grams 150 to 200 garms
23.
24. Cost estimates for cultivation of rapeseed (per
Traditional method SMI method
unit price/unit no. unit cost no. unit
costSeed kilogram 80 5.5 440 0.25
20Seed treatment lump sum 50 0 0 1
50Labor man hour 30 0 0 1
30Nursery preparation man day 250 0 0 0.5
125Seed & nursery preparation 440
225Field preparation
Ploughing (rent for unit 400 2 800 2
800
plough & buffalo)Labor man day 250 2 500 2
500Pit digging man day 250 0 4 100
Field preparation 1300 23
DAP kilogram 15 27 405 27
405MOP kilogram 18 27 486 27
486UREA kilogram 7 55 385 55 38
Biozyme kilogram 30 0 0 13.5 40
PSB kilogram 65 0 0 6 3
Vermicompost kilogram 5 0 0 400 200
Nutrient input 979 1
25. Continued.. Trad. Method SMI metho
unit price/unit no. unit cost no. unit co
Weeding &soil work man day 250 2 500 15 37
Weed control total 500
3750Plant protection
Chemical & pesticides lump sum 200 1 200 1 200
Labor man days 250 1 250 1 25
Rent of sprayer lump sum 100 1 100 1 1
Crop protection costs total 550
550Harvesting man days 250 13.5 3375 6
1500
Threshing & packing man days 250 13.5 3375 25
6250
Harvesting cost 2700
7750Capital cost 5000
Total operational costs total
Irrigation cost 900
2200
12666 24446
Management cost (10% of total) 1266
2444Cost of production 13932
26890Yield Rp/kg 35 500 17500 1200 420
Dry matter(straw, Rp/kg 2 950 1900 4050 810
fodder, ect.)
Gross income 19400
27. Mustard yields achieved with SMI metho
During 2009-10, the 7 women farmers from Gaya district
who applied SRI principles to rapeseed produced, on an
average, 1.2 tons per acre compared to 0.5 ton achieved
with traditional methods (2.5 tons per hactare vs. 1 ton).
During 2010-11, 273 farmers from Gaya and Nalanda
Districts had 1.3 tons per acre on average. This increased
to 1.4 tonnes per acre when SMI was done by 1,636 women
farmers in 2011-12.
Those farmers who applied all the principles of SRI and who
gave six irrigations achieved an average yield of more than
1.6 tons per acre (4 tons per hectare).
The maximum yield achieved so far by adopting SRI principles
was 1.97 tons per acre. This was triple the 0.67 ton achieved
with the traditional methods.
28. Shri Dilip Singh Gond
Village-Kachhwar, Block- Pali,
Dist.- Umaria (M.P.)
• Area - 1.00 acre
• Seed Rate - 100 g/acre
• Row to Row Distance - 90 cm
• Plant to Plant Distance - 90 cm
• Weed Control - Hand weeding with
Kudal & Conoweeder(2-3)
• Production - 19.00 Qtl/acre.
Traditional - 05.00 Qtl/acre.
• Cost of Cultivation - Rs. 16127/-
• Total Income - Rs. 63649/-
• Net Income - Rs. 47522/-
Success Story – SRI in Mustard
(System of Root Intensification in Mustard)
30. SMI can enhance grain yield as much as double
sustaining the ever burgeoning population.
Seed treatment with mixture of jaggery, cow urine &
vermi-compost, fungicides, and biofertilizers in an
order enhances soil fertility and soil quality.
Minimum chemical use sustains soil ecology.
Conclusion