Southern blotting is a technique used to detect specific DNA sequences within DNA samples. It involves digesting DNA samples with restriction enzymes, separating the fragments via gel electrophoresis, transferring the fragments to a membrane, and using radioactive probes to hybridize to complementary DNA sequences, which are then visualized. The key steps are digesting DNA, separating fragments by size via gel electrophoresis, transferring fragments to a membrane, hybridizing with radioactive probes, and detecting hybridized probes.