SlideShare a Scribd company logo
By: Corey Cotsonas
   Help to find molecular weight of a restriction
    fragment

   Detection of a specific DNA sequence
   Discovered by Edwin Southern in 1973

   Discovered at Edinburgh University

   Edwin’s work wasn’t published until 1975
   1) DNA and a restriction enzyme are mixed
    ◦ They are then separated by gel electrophoresis
    ◦ DNA is separated into single strands through
      incubation with NaOH


   2) DNA is transferred onto special blotting
    paper
    ◦ DNA fragments remain separated
   3) The blot is incubated with copies of a
    probe (single stranded DNA)
    ◦ Probe will bind with complimentary DNA sequence
    ◦ Creates double-stranded DNA molecule


   4) Location of probe is shown
    ◦ Revealed by incubating probe with colorless
      substrate, and exposes X-ray film
   http://highered.mcgraw-
    hill.com/sites/0072556781/student_view0/chapter14/animation_quiz_5.html

   http://www.sinauer.com/cooper5e/animation0411.html
   Northern and Southern Blot:
    ◦ Both use gel electrophoresis
    ◦ Northern blot identifies sequences in RNA and
      separates RNA fragments using the gel

                          Northern Blot 
   Western and Southern Blot:
    ◦ Also uses gel electrophoresis
    ◦ Western Blot used to find proteins and arrange
      them by size and shape.
                        Western Blot 

More Related Content

What's hot

Southern blot
Southern blotSouthern blot
Southern blot
smitty3896
 
Dna fingerprinting
Dna fingerprintingDna fingerprinting
Dna fingerprinting
sherwen
 
Southern blotting
Southern blottingSouthern blotting
Southern blotting
Rafa Zubair
 
Blotting techniques ppt
Blotting techniques pptBlotting techniques ppt
Blotting techniques ppt
Priya Soni
 
Southern Blotting
Southern BlottingSouthern Blotting
Southern Blotting
Anik Banik
 
Southern blot
Southern blot Southern blot
Southern blot
Khaeisya Jerry
 
Northern blotting
Northern blottingNorthern blotting
Northern blotting
Sachin Patil
 
Southern blot kanchana
Southern blot kanchanaSouthern blot kanchana
Southern blot kanchana
kanchana Sivabalan
 
SOUTHERN BLOTTING SMG
SOUTHERN BLOTTING  SMGSOUTHERN BLOTTING  SMG
SOUTHERN BLOTTING SMG
sajigeorge64
 
Southern hybridization
Southern hybridizationSouthern hybridization
Southern hybridization
Anushi Jain
 
Blotting type and uses
Blotting type and usesBlotting type and uses
Blotting type and uses
abokian
 
Southern blotting
Southern blottingSouthern blotting
Southern blotting
Muhammed Ali Saske
 
Southern blotting
Southern blottingSouthern blotting
Southern blotting
YogeshBhandari12
 
Northern Blotting technique
Northern Blotting technique Northern Blotting technique
Northern Blotting technique
Md.Emam Hasan
 
Southern blotting
Southern blottingSouthern blotting
Southern blotting
Akansha Soni
 
Southern blotting
Southern blottingSouthern blotting
Southern blotting
Health Tech Facts
 
Northern blooting
Northern blootingNorthern blooting
Northern blooting
Babar khan
 
Northern blotting
Northern blottingNorthern blotting
Northern blotting
Nabajit Roy
 
Southern Blotting Technique
Southern Blotting Technique Southern Blotting Technique
Southern Blotting Technique
Dr.M.Prasad Naidu
 
Southern Blotting and Related DNA Detection Techniques
Southern Blotting and  Related DNA Detection Techniques  Southern Blotting and  Related DNA Detection Techniques
Southern Blotting and Related DNA Detection Techniques
MD ASIQUR RAHMAN
 

What's hot (20)

Southern blot
Southern blotSouthern blot
Southern blot
 
Dna fingerprinting
Dna fingerprintingDna fingerprinting
Dna fingerprinting
 
Southern blotting
Southern blottingSouthern blotting
Southern blotting
 
Blotting techniques ppt
Blotting techniques pptBlotting techniques ppt
Blotting techniques ppt
 
Southern Blotting
Southern BlottingSouthern Blotting
Southern Blotting
 
Southern blot
Southern blot Southern blot
Southern blot
 
Northern blotting
Northern blottingNorthern blotting
Northern blotting
 
Southern blot kanchana
Southern blot kanchanaSouthern blot kanchana
Southern blot kanchana
 
SOUTHERN BLOTTING SMG
SOUTHERN BLOTTING  SMGSOUTHERN BLOTTING  SMG
SOUTHERN BLOTTING SMG
 
Southern hybridization
Southern hybridizationSouthern hybridization
Southern hybridization
 
Blotting type and uses
Blotting type and usesBlotting type and uses
Blotting type and uses
 
Southern blotting
Southern blottingSouthern blotting
Southern blotting
 
Southern blotting
Southern blottingSouthern blotting
Southern blotting
 
Northern Blotting technique
Northern Blotting technique Northern Blotting technique
Northern Blotting technique
 
Southern blotting
Southern blottingSouthern blotting
Southern blotting
 
Southern blotting
Southern blottingSouthern blotting
Southern blotting
 
Northern blooting
Northern blootingNorthern blooting
Northern blooting
 
Northern blotting
Northern blottingNorthern blotting
Northern blotting
 
Southern Blotting Technique
Southern Blotting Technique Southern Blotting Technique
Southern Blotting Technique
 
Southern Blotting and Related DNA Detection Techniques
Southern Blotting and  Related DNA Detection Techniques  Southern Blotting and  Related DNA Detection Techniques
Southern Blotting and Related DNA Detection Techniques
 

Viewers also liked

Southern blotting
Southern blottingSouthern blotting
Southern blotting
Shiamgowtham Reborn
 
Blotting techniques
Blotting techniquesBlotting techniques
Blotting techniques
Dr.M.Prasad Naidu
 
Southern northern and western blotting
Southern northern and western blottingSouthern northern and western blotting
Southern northern and western blotting
research
 
Blotting techniques includes southren,northern,western and dot blotting
Blotting techniques includes southren,northern,western and dot blottingBlotting techniques includes southren,northern,western and dot blotting
Blotting techniques includes southren,northern,western and dot blotting
bbmy
 
Western Blot
Western BlotWestern Blot
Western Blot
136659145
 
Northern blotting
Northern blottingNorthern blotting
Northern blotting
Akshay Pareek
 
Fluorescent in-situ Hybridization (FISH)
Fluorescent in-situ Hybridization (FISH)Fluorescent in-situ Hybridization (FISH)
Fluorescent in-situ Hybridization (FISH)
BioGenex
 
Northern blotting
Northern blottingNorthern blotting
Northern blotting
Dr. Abdul Wahab M. Kannde
 
Northern blotting
Northern blottingNorthern blotting
Northern blotting
mujahid hussain
 
Western blotting
Western blottingWestern blotting
Western blotting
Madiha Yusufzai
 
Blotting techniques (manish)
Blotting techniques (manish)Blotting techniques (manish)
Blotting techniques (manish)
manish chovatiya
 
Western blot.
Western blot.Western blot.
Western blot.
Yamileth Saldaña
 
Southern Blot
Southern BlotSouthern Blot
Southern Blot
Karlantonetti
 
Polymerase chain reaction
Polymerase chain reactionPolymerase chain reaction
Polymerase chain reaction
Aisha Kalsoom
 
Western blot
Western blotWestern blot
Western blot
diefer1
 
Blotting Techniques
Blotting TechniquesBlotting Techniques
Blotting Techniques
Kurgat Gilbert
 
Fundamentos de técnicas blotting: Southern, Northern, Western.
Fundamentos de técnicas  blotting: Southern,  Northern, Western. Fundamentos de técnicas  blotting: Southern,  Northern, Western.
Fundamentos de técnicas blotting: Southern, Northern, Western.
Carolina Herrera
 
Dna fingerprinting
Dna fingerprintingDna fingerprinting
Dna fingerprinting
IshaqueAbdulla
 
Microarray
MicroarrayMicroarray
Microarray
ruchibioinfo
 

Viewers also liked (20)

Southern blotting
Southern blottingSouthern blotting
Southern blotting
 
Blotting techniques
Blotting techniquesBlotting techniques
Blotting techniques
 
Southern northern and western blotting
Southern northern and western blottingSouthern northern and western blotting
Southern northern and western blotting
 
Blotting techniques includes southren,northern,western and dot blotting
Blotting techniques includes southren,northern,western and dot blottingBlotting techniques includes southren,northern,western and dot blotting
Blotting techniques includes southren,northern,western and dot blotting
 
Western Blot
Western BlotWestern Blot
Western Blot
 
Northern blotting
Northern blottingNorthern blotting
Northern blotting
 
Nothern blot
Nothern blotNothern blot
Nothern blot
 
Fluorescent in-situ Hybridization (FISH)
Fluorescent in-situ Hybridization (FISH)Fluorescent in-situ Hybridization (FISH)
Fluorescent in-situ Hybridization (FISH)
 
Northern blotting
Northern blottingNorthern blotting
Northern blotting
 
Northern blotting
Northern blottingNorthern blotting
Northern blotting
 
Western blotting
Western blottingWestern blotting
Western blotting
 
Blotting techniques (manish)
Blotting techniques (manish)Blotting techniques (manish)
Blotting techniques (manish)
 
Western blot.
Western blot.Western blot.
Western blot.
 
Southern Blot
Southern BlotSouthern Blot
Southern Blot
 
Polymerase chain reaction
Polymerase chain reactionPolymerase chain reaction
Polymerase chain reaction
 
Western blot
Western blotWestern blot
Western blot
 
Blotting Techniques
Blotting TechniquesBlotting Techniques
Blotting Techniques
 
Fundamentos de técnicas blotting: Southern, Northern, Western.
Fundamentos de técnicas  blotting: Southern,  Northern, Western. Fundamentos de técnicas  blotting: Southern,  Northern, Western.
Fundamentos de técnicas blotting: Southern, Northern, Western.
 
Dna fingerprinting
Dna fingerprintingDna fingerprinting
Dna fingerprinting
 
Microarray
MicroarrayMicroarray
Microarray
 

Similar to Southern blot method

VBC-321_BLOTTING_TECHNIQUES and gel electrophoresis
VBC-321_BLOTTING_TECHNIQUES and gel electrophoresisVBC-321_BLOTTING_TECHNIQUES and gel electrophoresis
VBC-321_BLOTTING_TECHNIQUES and gel electrophoresis
YoGeshSharma834784
 
VBC-321_BLOTTING_TECHNIQUES.pptx
VBC-321_BLOTTING_TECHNIQUES.pptxVBC-321_BLOTTING_TECHNIQUES.pptx
VBC-321_BLOTTING_TECHNIQUES.pptx
SajadBhat46
 
Blotting techniques
Blotting  techniquesBlotting  techniques
Blotting techniques
Varta Rani
 
Southern blotting-1.pptx
Southern  blotting-1.pptxSouthern  blotting-1.pptx
Southern blotting-1.pptx
HurAbbasBajwa
 
Blotting techniques
Blotting techniquesBlotting techniques
Blotting techniques
Dipesh Tamrakar
 
Técnicas
TécnicasTécnicas
Técnicas
Ana Batista
 
blottingtechniques.pptx different types of blotting technique
blottingtechniques.pptx different types of blotting techniqueblottingtechniques.pptx different types of blotting technique
blottingtechniques.pptx different types of blotting technique
TamannaNaidu
 
blotting techniques southern northern Western
blotting techniques southern northern Westernblotting techniques southern northern Western
blotting techniques southern northern Western
TamannaNaidu
 
Blotting technique
Blotting techniqueBlotting technique
Blotting technique
murtadha ali
 
Blotting techniques
Blotting techniquesBlotting techniques
Blotting techniques
OmBagade1
 
Blotting techniques
Blotting techniquesBlotting techniques
Blotting techniques
Kashikant Yadav
 
Blotting techniques
Blotting techniquesBlotting techniques
Blotting techniques
Kashikant Yadav
 
Southern blotting Technique (Nucleic acid hybridization).pptx
Southern blotting Technique (Nucleic acid hybridization).pptxSouthern blotting Technique (Nucleic acid hybridization).pptx
Southern blotting Technique (Nucleic acid hybridization).pptx
Krushna Doke
 
Southern and northern blotting technique
Southern and northern blotting techniqueSouthern and northern blotting technique
Blotting & its types
Blotting & its typesBlotting & its types
Blotting & its types
Vipin Shukla
 
Application of biotechnology in forensic
Application of biotechnology in forensicApplication of biotechnology in forensic
Application of biotechnology in forensic
MerinAliceGeorge
 
BLOTTING TECHNIQUES FOR RNA, DNA AND PROTEINS.pptx
BLOTTING TECHNIQUES FOR RNA, DNA AND PROTEINS.pptxBLOTTING TECHNIQUES FOR RNA, DNA AND PROTEINS.pptx
BLOTTING TECHNIQUES FOR RNA, DNA AND PROTEINS.pptx
TadiwanasheMagombe
 
Genetic methods of microbial taxonomy
Genetic methods of microbial taxonomyGenetic methods of microbial taxonomy
Genetic methods of microbial taxonomy
KARTHIK REDDY C A
 
southern blot, western blot,blotting technique ,blotppt.pptx
southern blot, western blot,blotting technique ,blotppt.pptxsouthern blot, western blot,blotting technique ,blotppt.pptx
southern blot, western blot,blotting technique ,blotppt.pptx
uziaftab5
 
Blotting techniques1
Blotting techniques1Blotting techniques1
Blotting techniques1
Tapeshwar Yadav
 

Similar to Southern blot method (20)

VBC-321_BLOTTING_TECHNIQUES and gel electrophoresis
VBC-321_BLOTTING_TECHNIQUES and gel electrophoresisVBC-321_BLOTTING_TECHNIQUES and gel electrophoresis
VBC-321_BLOTTING_TECHNIQUES and gel electrophoresis
 
VBC-321_BLOTTING_TECHNIQUES.pptx
VBC-321_BLOTTING_TECHNIQUES.pptxVBC-321_BLOTTING_TECHNIQUES.pptx
VBC-321_BLOTTING_TECHNIQUES.pptx
 
Blotting techniques
Blotting  techniquesBlotting  techniques
Blotting techniques
 
Southern blotting-1.pptx
Southern  blotting-1.pptxSouthern  blotting-1.pptx
Southern blotting-1.pptx
 
Blotting techniques
Blotting techniquesBlotting techniques
Blotting techniques
 
Técnicas
TécnicasTécnicas
Técnicas
 
blottingtechniques.pptx different types of blotting technique
blottingtechniques.pptx different types of blotting techniqueblottingtechniques.pptx different types of blotting technique
blottingtechniques.pptx different types of blotting technique
 
blotting techniques southern northern Western
blotting techniques southern northern Westernblotting techniques southern northern Western
blotting techniques southern northern Western
 
Blotting technique
Blotting techniqueBlotting technique
Blotting technique
 
Blotting techniques
Blotting techniquesBlotting techniques
Blotting techniques
 
Blotting techniques
Blotting techniquesBlotting techniques
Blotting techniques
 
Blotting techniques
Blotting techniquesBlotting techniques
Blotting techniques
 
Southern blotting Technique (Nucleic acid hybridization).pptx
Southern blotting Technique (Nucleic acid hybridization).pptxSouthern blotting Technique (Nucleic acid hybridization).pptx
Southern blotting Technique (Nucleic acid hybridization).pptx
 
Southern and northern blotting technique
Southern and northern blotting techniqueSouthern and northern blotting technique
Southern and northern blotting technique
 
Blotting & its types
Blotting & its typesBlotting & its types
Blotting & its types
 
Application of biotechnology in forensic
Application of biotechnology in forensicApplication of biotechnology in forensic
Application of biotechnology in forensic
 
BLOTTING TECHNIQUES FOR RNA, DNA AND PROTEINS.pptx
BLOTTING TECHNIQUES FOR RNA, DNA AND PROTEINS.pptxBLOTTING TECHNIQUES FOR RNA, DNA AND PROTEINS.pptx
BLOTTING TECHNIQUES FOR RNA, DNA AND PROTEINS.pptx
 
Genetic methods of microbial taxonomy
Genetic methods of microbial taxonomyGenetic methods of microbial taxonomy
Genetic methods of microbial taxonomy
 
southern blot, western blot,blotting technique ,blotppt.pptx
southern blot, western blot,blotting technique ,blotppt.pptxsouthern blot, western blot,blotting technique ,blotppt.pptx
southern blot, western blot,blotting technique ,blotppt.pptx
 
Blotting techniques1
Blotting techniques1Blotting techniques1
Blotting techniques1
 

Recently uploaded

Climate Impact of Software Testing at Nordic Testing Days
Climate Impact of Software Testing at Nordic Testing DaysClimate Impact of Software Testing at Nordic Testing Days
Climate Impact of Software Testing at Nordic Testing Days
Kari Kakkonen
 
Video Streaming: Then, Now, and in the Future
Video Streaming: Then, Now, and in the FutureVideo Streaming: Then, Now, and in the Future
Video Streaming: Then, Now, and in the Future
Alpen-Adria-Universität
 
Best 20 SEO Techniques To Improve Website Visibility In SERP
Best 20 SEO Techniques To Improve Website Visibility In SERPBest 20 SEO Techniques To Improve Website Visibility In SERP
Best 20 SEO Techniques To Improve Website Visibility In SERP
Pixlogix Infotech
 
Introduction to CHERI technology - Cybersecurity
Introduction to CHERI technology - CybersecurityIntroduction to CHERI technology - Cybersecurity
Introduction to CHERI technology - Cybersecurity
mikeeftimakis1
 
“I’m still / I’m still / Chaining from the Block”
“I’m still / I’m still / Chaining from the Block”“I’m still / I’m still / Chaining from the Block”
“I’m still / I’m still / Chaining from the Block”
Claudio Di Ciccio
 
GraphSummit Singapore | The Art of the Possible with Graph - Q2 2024
GraphSummit Singapore | The Art of the  Possible with Graph - Q2 2024GraphSummit Singapore | The Art of the  Possible with Graph - Q2 2024
GraphSummit Singapore | The Art of the Possible with Graph - Q2 2024
Neo4j
 
UiPath Test Automation using UiPath Test Suite series, part 6
UiPath Test Automation using UiPath Test Suite series, part 6UiPath Test Automation using UiPath Test Suite series, part 6
UiPath Test Automation using UiPath Test Suite series, part 6
DianaGray10
 
Driving Business Innovation: Latest Generative AI Advancements & Success Story
Driving Business Innovation: Latest Generative AI Advancements & Success StoryDriving Business Innovation: Latest Generative AI Advancements & Success Story
Driving Business Innovation: Latest Generative AI Advancements & Success Story
Safe Software
 
Goodbye Windows 11: Make Way for Nitrux Linux 3.5.0!
Goodbye Windows 11: Make Way for Nitrux Linux 3.5.0!Goodbye Windows 11: Make Way for Nitrux Linux 3.5.0!
Goodbye Windows 11: Make Way for Nitrux Linux 3.5.0!
SOFTTECHHUB
 
Uni Systems Copilot event_05062024_C.Vlachos.pdf
Uni Systems Copilot event_05062024_C.Vlachos.pdfUni Systems Copilot event_05062024_C.Vlachos.pdf
Uni Systems Copilot event_05062024_C.Vlachos.pdf
Uni Systems S.M.S.A.
 
How to Get CNIC Information System with Paksim Ga.pptx
How to Get CNIC Information System with Paksim Ga.pptxHow to Get CNIC Information System with Paksim Ga.pptx
How to Get CNIC Information System with Paksim Ga.pptx
danishmna97
 
みなさんこんにちはこれ何文字まで入るの?40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの?えこ...
みなさんこんにちはこれ何文字まで入るの?40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの?えこ...みなさんこんにちはこれ何文字まで入るの?40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの?えこ...
みなさんこんにちはこれ何文字まで入るの?40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの?えこ...
名前 です男
 
Infrastructure Challenges in Scaling RAG with Custom AI models
Infrastructure Challenges in Scaling RAG with Custom AI modelsInfrastructure Challenges in Scaling RAG with Custom AI models
Infrastructure Challenges in Scaling RAG with Custom AI models
Zilliz
 
20240607 QFM018 Elixir Reading List May 2024
20240607 QFM018 Elixir Reading List May 202420240607 QFM018 Elixir Reading List May 2024
20240607 QFM018 Elixir Reading List May 2024
Matthew Sinclair
 
Artificial Intelligence for XMLDevelopment
Artificial Intelligence for XMLDevelopmentArtificial Intelligence for XMLDevelopment
Artificial Intelligence for XMLDevelopment
Octavian Nadolu
 
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with SlackLet's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
shyamraj55
 
UiPath Test Automation using UiPath Test Suite series, part 5
UiPath Test Automation using UiPath Test Suite series, part 5UiPath Test Automation using UiPath Test Suite series, part 5
UiPath Test Automation using UiPath Test Suite series, part 5
DianaGray10
 
“Building and Scaling AI Applications with the Nx AI Manager,” a Presentation...
“Building and Scaling AI Applications with the Nx AI Manager,” a Presentation...“Building and Scaling AI Applications with the Nx AI Manager,” a Presentation...
“Building and Scaling AI Applications with the Nx AI Manager,” a Presentation...
Edge AI and Vision Alliance
 
Serial Arm Control in Real Time Presentation
Serial Arm Control in Real Time PresentationSerial Arm Control in Real Time Presentation
Serial Arm Control in Real Time Presentation
tolgahangng
 
Why You Should Replace Windows 11 with Nitrux Linux 3.5.0 for enhanced perfor...
Why You Should Replace Windows 11 with Nitrux Linux 3.5.0 for enhanced perfor...Why You Should Replace Windows 11 with Nitrux Linux 3.5.0 for enhanced perfor...
Why You Should Replace Windows 11 with Nitrux Linux 3.5.0 for enhanced perfor...
SOFTTECHHUB
 

Recently uploaded (20)

Climate Impact of Software Testing at Nordic Testing Days
Climate Impact of Software Testing at Nordic Testing DaysClimate Impact of Software Testing at Nordic Testing Days
Climate Impact of Software Testing at Nordic Testing Days
 
Video Streaming: Then, Now, and in the Future
Video Streaming: Then, Now, and in the FutureVideo Streaming: Then, Now, and in the Future
Video Streaming: Then, Now, and in the Future
 
Best 20 SEO Techniques To Improve Website Visibility In SERP
Best 20 SEO Techniques To Improve Website Visibility In SERPBest 20 SEO Techniques To Improve Website Visibility In SERP
Best 20 SEO Techniques To Improve Website Visibility In SERP
 
Introduction to CHERI technology - Cybersecurity
Introduction to CHERI technology - CybersecurityIntroduction to CHERI technology - Cybersecurity
Introduction to CHERI technology - Cybersecurity
 
“I’m still / I’m still / Chaining from the Block”
“I’m still / I’m still / Chaining from the Block”“I’m still / I’m still / Chaining from the Block”
“I’m still / I’m still / Chaining from the Block”
 
GraphSummit Singapore | The Art of the Possible with Graph - Q2 2024
GraphSummit Singapore | The Art of the  Possible with Graph - Q2 2024GraphSummit Singapore | The Art of the  Possible with Graph - Q2 2024
GraphSummit Singapore | The Art of the Possible with Graph - Q2 2024
 
UiPath Test Automation using UiPath Test Suite series, part 6
UiPath Test Automation using UiPath Test Suite series, part 6UiPath Test Automation using UiPath Test Suite series, part 6
UiPath Test Automation using UiPath Test Suite series, part 6
 
Driving Business Innovation: Latest Generative AI Advancements & Success Story
Driving Business Innovation: Latest Generative AI Advancements & Success StoryDriving Business Innovation: Latest Generative AI Advancements & Success Story
Driving Business Innovation: Latest Generative AI Advancements & Success Story
 
Goodbye Windows 11: Make Way for Nitrux Linux 3.5.0!
Goodbye Windows 11: Make Way for Nitrux Linux 3.5.0!Goodbye Windows 11: Make Way for Nitrux Linux 3.5.0!
Goodbye Windows 11: Make Way for Nitrux Linux 3.5.0!
 
Uni Systems Copilot event_05062024_C.Vlachos.pdf
Uni Systems Copilot event_05062024_C.Vlachos.pdfUni Systems Copilot event_05062024_C.Vlachos.pdf
Uni Systems Copilot event_05062024_C.Vlachos.pdf
 
How to Get CNIC Information System with Paksim Ga.pptx
How to Get CNIC Information System with Paksim Ga.pptxHow to Get CNIC Information System with Paksim Ga.pptx
How to Get CNIC Information System with Paksim Ga.pptx
 
みなさんこんにちはこれ何文字まで入るの?40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの?えこ...
みなさんこんにちはこれ何文字まで入るの?40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの?えこ...みなさんこんにちはこれ何文字まで入るの?40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの?えこ...
みなさんこんにちはこれ何文字まで入るの?40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの?えこ...
 
Infrastructure Challenges in Scaling RAG with Custom AI models
Infrastructure Challenges in Scaling RAG with Custom AI modelsInfrastructure Challenges in Scaling RAG with Custom AI models
Infrastructure Challenges in Scaling RAG with Custom AI models
 
20240607 QFM018 Elixir Reading List May 2024
20240607 QFM018 Elixir Reading List May 202420240607 QFM018 Elixir Reading List May 2024
20240607 QFM018 Elixir Reading List May 2024
 
Artificial Intelligence for XMLDevelopment
Artificial Intelligence for XMLDevelopmentArtificial Intelligence for XMLDevelopment
Artificial Intelligence for XMLDevelopment
 
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with SlackLet's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
 
UiPath Test Automation using UiPath Test Suite series, part 5
UiPath Test Automation using UiPath Test Suite series, part 5UiPath Test Automation using UiPath Test Suite series, part 5
UiPath Test Automation using UiPath Test Suite series, part 5
 
“Building and Scaling AI Applications with the Nx AI Manager,” a Presentation...
“Building and Scaling AI Applications with the Nx AI Manager,” a Presentation...“Building and Scaling AI Applications with the Nx AI Manager,” a Presentation...
“Building and Scaling AI Applications with the Nx AI Manager,” a Presentation...
 
Serial Arm Control in Real Time Presentation
Serial Arm Control in Real Time PresentationSerial Arm Control in Real Time Presentation
Serial Arm Control in Real Time Presentation
 
Why You Should Replace Windows 11 with Nitrux Linux 3.5.0 for enhanced perfor...
Why You Should Replace Windows 11 with Nitrux Linux 3.5.0 for enhanced perfor...Why You Should Replace Windows 11 with Nitrux Linux 3.5.0 for enhanced perfor...
Why You Should Replace Windows 11 with Nitrux Linux 3.5.0 for enhanced perfor...
 

Southern blot method

  • 2. Help to find molecular weight of a restriction fragment  Detection of a specific DNA sequence
  • 3. Discovered by Edwin Southern in 1973  Discovered at Edinburgh University  Edwin’s work wasn’t published until 1975
  • 4.
  • 5.
  • 6.
  • 7. 1) DNA and a restriction enzyme are mixed ◦ They are then separated by gel electrophoresis ◦ DNA is separated into single strands through incubation with NaOH  2) DNA is transferred onto special blotting paper ◦ DNA fragments remain separated
  • 8. 3) The blot is incubated with copies of a probe (single stranded DNA) ◦ Probe will bind with complimentary DNA sequence ◦ Creates double-stranded DNA molecule  4) Location of probe is shown ◦ Revealed by incubating probe with colorless substrate, and exposes X-ray film
  • 9. http://highered.mcgraw- hill.com/sites/0072556781/student_view0/chapter14/animation_quiz_5.html  http://www.sinauer.com/cooper5e/animation0411.html
  • 10. Northern and Southern Blot: ◦ Both use gel electrophoresis ◦ Northern blot identifies sequences in RNA and separates RNA fragments using the gel Northern Blot 
  • 11. Western and Southern Blot: ◦ Also uses gel electrophoresis ◦ Western Blot used to find proteins and arrange them by size and shape. Western Blot 