Coulomb blockade occurs when the electrostatic energy required to add a single electron to a microscopic conductor is greater than the thermal energy. This results in a gap in the conductor's energy levels and prevents electron tunneling below a threshold voltage that depends on temperature. A single electron transistor uses controlled electron tunneling between a source and drain electrode connected by a quantum dot or wire channel to amplify current. It operates by changing the quantum system's energy levels with a gate voltage to allow electrons to tunnel one by one. Mathematical modeling of the single electron transistor involves calculating tunneling rates and probabilities based on free energy changes from electron additions or removals.