International edition of Shojinmeat Project overview
Shojinmeat Project is a citizen science project that develop DIY cell-based meat and engage in public communication for cellular agriculture.
2. “Shojinmeat Project” - Who we are
“Democratization of cellular agriculture”
Nonprofit non-corporate non-university citizen science community of DIY bio/fab
enthusiasts, students, researcher, artists, writers etc. for cellular agriculture
Open source cellular agriculture” by DIY bio Public communication by art and education
3. Ongoing projects
・DIY bio & cell culture experiments
・Workshops and micro-conferences
・Advocacy for cellular agriculture
・Media and social communication
・Art, design and creativity projects
・Visual contents production
・Fundraising / crowdfunding
4. Means of food production
Hunting Farming Domestication
Fermentation Synthesis Cell culture
6. From where?
Meat is ~x40 more resource intensive
Lamb:~x50, Beef:~x40, Pork:~x20, Poultry:~x7
“Meat”←animals←feed, water, land
7. Deforestation Public health hazard Water shortage
“Meat”←animals←feed, water, land
Hoekstra, Mekonnen, PNAS 2012
http://www.pnas.org/content/109/9/3232
Poore, Nemecek, Science 2018
http://science.sciencemag.org/content/360/6392/987
Ventola, NCBI 2015,
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4378521/
8. Food vs. Feed vs. Fuel
Agri-
cultural
resources
Food
Feed
Fuel Poore, Nemecek, Science 2018
http://science.sciencemag.org/content/360/6392/987
9. Anticipated alternative proteins
*As demand for protein grows, existing meat
cannot serve all in sustainable manner.
MeatSoy etc. Dairy
MeatDairySoy etc. New alternative protein
Plants
Tofu
Algae Insects Biosynthetic Cell culture
New protein
source
“Meat & dairy produced
in new ways”
Plant-based meat &
dairy equivalent
Now
Future
13. Ongoing cellular agriculture projects
Cell-based meat Milk without cows Egg white without eggs
The products are not “imitations” - they are (or try to be) molecularly the same!
Clean
fish
meat
Ginkgo Bioworks -
Vanilla, scent, various
ingredients
Modern Meadow -
leather without
animals
15. Research track record 1
1997
Goldfish meat
@NASA
Appearance in
sci-fi - Concept
known since
19th century
2004
New
Harvest
founded
Jason
Matheny
contacts
NASA staff
2005
Netherlands
funds €2M
2007
In vitro meat
consortium
funding
discontinued
funds
2000
Works by
Oron Catts
@Harvard
Lead by Willem van Eelen
(deceased)
Patent filed in 1997
16. Research track record 2
2012
Sergey Brin from
Google contacts
former member of
In vitro meat
consortium
2013
Demonstration
by Prof. Post
2014
Shojinmeat
Project
2015
Memphis
Meats
2015
“Cellular agriculture”
- term coined
2013
New Harvest invests
in cell-ag startups
(Clara Foods, Perfect Day)
2016
SuperMeat
2017
Finless
Foods
€280k burger
17. The positive impact
學
經
藝
Science & Technology
・Technological hurdles?
・Medical applications?
Politics & Economics
・Shift in food market?
・Industry landscape?
Arts and Culture
・Religious views?
・Social norms to change?
Hanna Tuomisto 2011
19. Technological milestones
1. Inexpensive media
2. Scaling & automation
CapEx &
Staffing
Culture
medium
$200k+/kg
Conventional method
$2
Conventional
meat
price &
quality
parity?
3. Added value and
consumer acceptance
Technological goals
1. Food grade culture medium
2. Scaled culture plant design
3. Tissue engineering for flavor
and texture
20. Bottlenecks in culture medium
Gospodarowicz D and Moran JS, 1976, Annu Rev Biochem Eagle H, 1959, Science
Sugar, Amino acids,
Vitamins, Minerals
$20/L
Expensive for what’s
actually in
Albumin, Buffer,
Insulin, Transferin
$900/L
“Mad cow”? Viruses?
Expensive and supply
is insecure
Growth factors
Survival factors
$450/mg
Expensive… :-(
=
Basal
medium
Foetal
bovine
serum
Signal
compounds
21. Cost of culture media for “easy” cells
Standard DMEM(FBS10%) 500ml
$5000+ for 100g
Medium for 1~2g of cells
(yen/JPY)
22. Liver cell culture for therapeutic applications
Block et al., 1996
DMEM 450ml ¥1125
FBS 50ml ¥4900
Non essential amino acid ¥140
HGF 40ng/ml ¥78000 (20µg)
EGF 20ng/ml ¥700 (10µg)
¥84865($8000)
100g of liver cells cost ~$100,000
23. Cell-based meat production - Method 1
Glocose, amino acids,
AA2-P, NaHCO3, Na2Se
These (expensive) growth factors
will be made by recombinants
(in the same way insulin is made)
20~200kL cell
cultivator tank
~3t scale
production
Insulin, Transferin,
FGF2, TGF-b
“Essential 8”
cell-based
meat medium Growth factors
&
FBS ingredients
“All-in-1 batch”
24. Cost of goods (ingredients) analysis
Medium Meat
$41/L $100/lb
$15/L $36.6/lb
$4.7/L $11.5/lb
$3.7/L $9.0/lb
$0.77/L $2.2/lb
Scenario A~E
A: All GF’s down to 1/10 in cost
B: FGF2 & TGFβ at insulin price
C: A&B
D: All GF’s at $4/g
E: Basal medium at $0.23/L
Good Food Institute (2016)
(“All in one batch” process is assumed)
25. Cell-based meat production - Method 2
⇒$0/L
Originally $1500/L
Produces
growth factors
Muscle cells Liver cells
growth
factorsGF’s
&
FBS eq’s
Basal
medium
Inexpensive
Very
expensive
Medium ingredients
“Coculture”
GF’s
&
FBS eq’s
26. Demonstration of coculture
Significant hepatocyte growth without
added growth factors (HGF)Control (0%) 10% conditioned
medium
25% conditioned
medium
50% conditioned
medium
Countofcellsofallsizes,
relativetothecontrol
group
mouse placental cells
dishes with Day-12 foetal liver cells in FBS 10% medium
7 Days
Transfer culture medium
34. The process needs a fundamental re-design
Labs, Hospitals
Brewery, Petrochemical complex
Culturing of cells has been optimised for laboratory scale
⇒€250k per burger
Culturing of cells becomes industrial scale
⇒Production at $2/kg
35. Dr. Marianne Ellis, 2017 “Process scale” method
implementation cases⇒
“Cell culture processes suitable for scaling”
36. Ready for plant engineering?
Integration of all elements into one scalable system
~0.1g scale ~10g scale ~100kg scale future pilot plant
PCT/JP2016/067599 jp-pat file# 2016-568716
37. How is temperature controlled?
Mixing method?
Pipeline diameter & flow rate?
Sterilization method & frequency?
How are filters cleaned?
Plant engineering - what exactly?
Speculative fish
meat culture plant
38. “Is it tasty?” - Tissue engineering to add value
Sausage/burger
Proven ※although expensive
Low cost large scale
cell culture
Sheet meat / “bacon”
Cell scaffold
Muscle/fat coculture
Steak / tissue
Tissue morphogenesis
Vascularization
Meat texture
Regenerative
medicine
Where we are
39. Tissue engineering, regenerative medicine and cellular agriculture
Cell culture
(human)
Cell culture
(animals)
Distribution
Distribution
Regenerative medicine Cellular agriculture
Med.
Ag.
Procu-
rement
Culture
medium
Cell-ag and Regen. Medicine share the same technology.
Main differences are in purity, traceability and regulations
40. Building “meat” : Method 1
“Opportunities for applying bio- medical
production and manufacturing methods
to the development of the clean meat
industry”
https://www.sciencedirect.com/science/articl
e/pii/S1369703X1830024XCulture medium
and starter cells
Myoblast
culture
Myogenesis,
Cell organization
Grow cells
Build tissue by
cellular scaffoldProcurement
41. 3D culture by cellular scaffolds
Cellular scaffold has large surface area, which improves the efficiency of cell culture.
Sponge collagen scaffolds Liver cells on scaffolds
42. Other functions of cellular scaffolds
Edible scaffolds i.e.
collagen, chitosan,
chitin, arginate, cellu-
lose, polysaccharides
Simulate fibre
and meat texture
Moulds shape in
mm or bigger
scales
43. Building “meat” : Method 2
”In Vitro Engineering of
Vascularized Tissue Surrogates”
https://www.nature.com/articles/srep01316
Culture medium
and starter cells
Procurement
Cell growth, vascularization
Tissue growth
※Further down the
development timeline
44. Tissue engineering to and beyond thick steak
Cells on scaffold
around 2020
Cell aggregate
2013
Cultured tissue
2026?
Designer
meat?
2030?
Burger,
sausage
Bacon,
meat chips
Thick steak “It this even
meat !?”
Algae-myoblast
co-culture
45. The positive impact
學
經
藝
Science & Technology
・Technological hurdles?
・Medical applications?
Politics & Economics
・Shift in food market?
・Industry landscape?
Arts and Culture
・Religious views?
・Social norms to change?
Hanna Tuomisto 2011
46. “Protein problem” in different stages
Is there
enough
protein?
Is it a
secure
source?
Is it
sustain-
able?
Many rely on
imports while
overfishing
continues
( Economic and Social Research Institute, Dublin, Ireland, 2010 https://www.esri.ie/pubs/WP340.pdf)
“Public expenses
due to meat is set to
reach $1.6T by 2050”
Farm Animal Investment Risk
and Return Initiative
Poor conturies
47. “Impeding food crisis”?
”Boiling frog"
・Food prices rise incrementally over decades
・Lower living standards, more frequent civil unrest
48. How cellular agriculture is a solution
1
2
Reduce protein
consumption.
Change the way
we eat.
Find sustainable
protein source.
Preserve
culinary culture.
Meat supply chain
Externalities
“Wicked problem” of climate change,
poverty and local ecological losses
49. Comprehensive cost and
footprint assessment
・Resource requirement
from ‘cradle to the grave’
Life cycle assessment (LCA)
https://rationalwiki.org/wiki/File:LCA.PNG
50. LCA uncertainties
Current LCA estimates depend on unestablished production
processes and uncertainties still may exceed 50%.
2014 estimate included
bioreactor sterilization.
Hanna Tuomisto 2011 Hanna Tuomisto 2014
51. “Matter cycle” of cell-based meat
Convert to culture media
Large-scale cell cultureWaste fluid treatment
Algal production
Algae
sewage
Fertilizer
52. Global protein market outlook
Population
growth and
emerging
economies:
$2T market cap.
by 2030?
Global meat demand 1980-2030
Rabobank (2011)■Lamb ■Poultry ■Pork ■Beef
Demand,10milliontons
53. Cellular agriculture startups and market size
By Olivia Fox Cabane, http://newprotein.org *Funders and supporters are also mentioned. IP summary: https://www.kiranmeats.com/resources/
Japan
$50B
Seafood $250B
Global meat
$1.5T
Global beef
$0.7T
54. Market timeline in Japan by Integriculture Inc.
Plans to market high unit
price products first and
move to general meat as
cell culture scales
Test Production
$300,000/kg
Foie Gras
$40/kg
“Designer
Meat”
$20/kg
i.e.
“fish fat beef”
“beef/pork cellular mix”
“DHA/Omega3 plus meat”
Meat.
$6/kg
Supplements
$3000/kg
2019 2023 2027 2030
55. Non-profits and academic institutions
USA
Israel
USA Japan
USA
Donor-funded 501(c)(3)
-Supports academic
research of cellular
agriculture
https://www.new-harvest.org/
https://media.dglab.com/2017/02/
09-joi-blog-01/
Founded by New Crop
Capital. Promotes
plant-based and cell-
based meat from
industry perspective.
https://www.gfi.org/
Funded by crowdfunding,
Promotes open-source DIY
cellular agriculture
http://shojinmeat.com
https://camp-fire.jp/projects/view/20537
Founded by university
students, aims to connect
academia and industry
https://www.gfi.org/
Spun off SuperMeat
https://www.futuremeat.org/
Grants
Supports New
Harvest
Demonstrated cell-
based meat in 2013
NPO’s play facilitative
role between the public,
regulators and industry.
Some are more
academic.and others
56. “Adjacent industries”
Entry from nearby fields
Beverage
companies
Food
companies
Medical supply
manufacturer
Plant engine-
ering firms
Effect to nearby fields
Functional &
pharmaceuti
cal foods
Food
branding
Biomanu
facturing
Regen.
medicine
Indoor
farms
Cell-based meat industry mind map
Good Food Institute (2016)
57. Political and regulatory environment
FDA public hearing held
on 12/07/2018 to resolve
the regulatory issue -
join hearing with USDA
planned for October.
https://www.fda.gov/Food/NewsEven
ts/WorkshopsMeetingsConferences/
ucm610138.htm
Set to spend €400M
on alternative protein
research from 2018.
All new food product
must go through 18
months long approval
procedure.
Some research works
are ongoing in
government labs and
set to invest $300M
on Israeli alternative
protein startups.
Government fund
invests in a cellular
agriculture startup.
Government official
moving to submit
policy advice.
58. Regulations and cellular agriculture products
A food product must be manufactured from food and (approved) food additives
under proper process control. A “food” must have history of consumption (⇔
“Novel food”). There is a list for (FDA, in case of USA) approved food additives.
cell-based meat
Growth factors
Could have a
GRAS status
Requires safety
tests and approval
if used.
Sugar
Amino acids
Vitamins
Minerals etc.
(Food & additives)
Basal
media
Basal media
Process
control:
HACCP
& GMP
Could use
sports drink
59. Where are the risks?
BSE prions?
Cells mix-ups?
Viral contamination?
Is it GMO?
Are growth factors safe?
What’s in the medium?
Bacterial contamination?
Cancerous cells?
Safety of cell metabolites?
Will it be labelled?
Unexpected health risk
from “too clean meat”?
Is meat healthy?
60. 細胞肉の食品安全衛生:対策例や争点
Use of primary cells or cell line cells?
Viral DNA can be monitored real-time in near future?
Can be avoided by cell acquisition from prion-free parts?
Use of cell sorter may separate by each cell types
Existing food regulations can evaluate the food safety of growth factors?
Use of methods that does not require external addition of growth factors?
Use of known medium? Prompt reporting of any changes made to medium?
https://www.sigmaaldrich.com/life-science/cell-culture/learning-center/media-formulations/dme.html
Would require contamination detection mechanism for quality assurance
“Substantial equivalence” - how do the amount of abnormal or cancerous
cells compare to existing meat?
How should it be labeled? “Beef(cell-based)” ?
Long-term safety - how do cell-based meat differ from conventional?
Is meat consumption healthy - regardless of traditional or cell-based?
61. Who will regulate meat? (And what is “meat”?)
Good Food Institute, Tofurky and a few alternative protein
startups filed a court case against State of Missouri
https://www.nytimes.com/2018/08/28/us/missouri-meat-law-tofurky.html
Petitions not to call
plant-based and cell-
based meat “meat”
State of Missouri passes a
food labeling law in favor
Who will regulate? FDA
or USDA? ⇒ Letter to the
President
62. The positive impact
學
經
藝
Science & Technology
・Technological hurdles?
・Medical applications?
Politics & Economics
・Shift in food market?
・Industry landscape?
Arts and Culture
・Religious views?
・Social norms to change?
Hanna Tuomisto 2011
66. Practical ethics: “Utilitarianism”
“Decision should be made to maximize utility”
Utility = happiness x number of sentient beings
“Only 1 death is better than 5”...?
May be so in very short term, but...
If “killing for public good” becomes the
norm, no one is there to stop dictators
→ Is the utility maximized, in the long
run?
Utilitarian decision making is strongly
dependent on subject and timeline“Runaway trolley problem”
As it is, 5 will die. If you switch, only 1 will. Will you do it?
67. Animal welfare as deductions from utilitarianism
◆Animals are capable of feeling happiness
and thus sentient.
◆All sentient beings count.
◆”Species” as defined by biology is irrelevant
in ethical decisions.
⇒From a utilitarian point-of-view,
“making sentient beings suffer is
unethical”
68. Acknowledges health and environment, but mainly animal welfare and ethical
“Animal welfare” based
on utilitarianism
Reason for being a vegetarian
Vegetarian Society
69. East Asian vegetarianism
”Religion”←Not utilitarianism or other ethics
Ethical value of clean meat described by utilitarianism don’t directly
translate into East Asian religious (i.e. Buddhist) importance.
70. “Shojin ryori” Buddhist cuisine
Cuisine for for zen practitioners
・Common name for “Buddhist cuisine”
・All aspects must serve the purpose of zen.
-NO WASTED FOOD
-Use local produce to avoid food waste
-No cruelty (avoid fish and meat)
-Avoid certain vegetables that stimulate desires
-All donated food including meat must be consumed
-Preparation (cooking) is also part of zen practice
Cell-based meat being “murderless” does not make it Zen - there are multiple more
important criteria.
※contested
“Shojin” means “devotion to the righteous path”
Shojinmeat Project will stay committed to the path that obsoletes unsustainable meat
71. Would “murderless meat” help Zen?
Holy text
↓
Rules
↓
Daily life
Religious rules in
Abrahamic religions
RulesMiddle way
Daily life
Holy texts
”Would clean meat serve the
pupose of Zen?”
・Overcoming personal desires is a
major theme in zen
・”Desire” includes meat apetite
・”Fake meat” is a compromise, but
compromise is permissible
(refer. “middle way”)⇒
・Cell-based meat fits in the same
category as tofu - meat imitations.
Use as reference up
on making decisions
Mentions
Religious rules in
Eastern religions
72. Meat in historical Japan
Before 7th century:
Eating meat was common. People just had to eat
whatever was in hand.
675c. Imperial decree of “No Killing (of animals)”
To direct labour force to rice production and put a stop to
local animal-sacrifice rituals & reinforce imperial authority
※Newly arrived Buddhism was used as justification
Meat avoidance continues till 19th c. and commoners
only started eating meat around 1900 c.
73. ・Totals half billion? Region-specific
・More common among upper castes
・Some upper caste members fund
cultured meat research
・Hinduism doesn’t explicitly forbid meat
but adherents choose to avoid meat.
Mr. Modi (Indian PM as
of 2017) is a vegetarian.
Vegetarianism in India
74. Potential future shift in ethical landscape
Then what if on 2040, meat alternatives rise to 30% market share and ever
more people stop consuming conventional meat?
・Uncontrollable ”hate campaign” against traditional farmers?
・Trade ban of conventional meat due to animal cruelty?
Why are animal experimentation,
Japanese whaling and Chinese cat/
dog consumption is problematised far
more than factory farming?
⇒Because they are “remote things” for
the protesters.
75. Ethical issues due to technological immaturity
(Transient) issues upon R&D:
・FBS production is not cruelty-free
“Unavoidable” issue:
・Extraction of cells
Likely to be solved in the future
May pose an issue during R&D
Can bovine foetus feel pain?
It may still inflict some pain.
Will animals still be chained?
Genetic selection of animals for the
sake of “tasty” - is it eugenics?
76. Ethical issues of “captive carnivore”
Wild animals exist outside human
control and no ethical questions
are raised against humans.
However, captive carnivores as pets
and zoo animals are under human
control → ethical questions
?? Is it ethical to let carnivores to prey on others??
77. Future court case: “Patent or Life (of animals)”
・Can rich countries with cell-based meat production technology blame
(poor) emerging countries for animal abuse?
・Court cases over “economic incentives (patent) vs. animal suffering”
Case study: Generic HIV drug lawsuit:
An Indian pharmaceutical company allegedly infringed
retroviral drug patent to manufacture generic HIV drugs,
because the original drugs by Western pharmaceutical
companies were too expensive for people in poor
African countries.
After high-profile court-martials, the Indian company
won the case on humanitarian basis.
78. Consumer acceptance
Chris Bryant (2017)
3rd Intl’ Conf. for Cultured Meat
Large variations exist between different marketing
research attempts and speculations, mainly due to
the lack of actual clean meat products on market.
←Factors
consumers
weigh
Consumer
acceptance
research result→
79. Nomenclature
”Clean meat”
cultured meat
(acknowledges “cell-based meat”)
Cell-based meat
and
coalition
純粋培養肉(純肉)、細胞(培養)肉
・How do general consumers perceive?
・Is it a neutral name?
・Does it comply with food labeling law?
Good Food Institute(2017)
(Japanese)
https://www.gfi.org/how-we-talk-about-meat-grown-without-animals
80. What is needed for public acceptance?
Demo by Memphis Meats
cell-based meat by DIY bio
http://www.nicovideo.jp/watch/sm30099092
Being
safe
(regulatory)
Feeling
safe
(cultural)
Technology is
coming but...
81. What would a corporate monopoly do?
Resulted in widespread accusation and allegations of :
“Technology being used for corporate profit than social good”
“Public mistrust due to technological secrecy”
82. Cellular agriculture ecosystem
Advocacy, Academic
research with universities
DIY bio, speculative art projects,
“avant-garde” advocacy
Sponsor
Research and project grants
Commercialization,
Production technology
development
Individual biohackers in
communities such as
83. Shojinmeat Project as citizen science
Non-Profit
organization
(TBC)
Citizen Science
Advocacy, Academic
research with universities
DIY bio, speculative art projects,
“avant-garde” advocacy
Sponsor
Research and project grants
(A spin-off startup)
Commercialization,
Production technology
development
84. Citizen science and business at Shojinmeat Project
How to make ⇒ Open
How to scale ⇒ Proprietary
Numberindemand
Degree of personalization
Product dev.
Citizen Science domain
Business domain
and other businesses
to come
Transparency
in technology
86. “Growing meat at home”
=DIY bio methodology=
Konjac cell scaffold
Cells from fertilized eggs
Egg white as antimycotic
Egg yolk as FBS
⇒DIY cell culture in kitchen
High school girl on DIY clean
meat experiment, TV news
DIY clean meat
recipe ‘Zine
(2017 Comiket92)
87. Development of DIY bio equipments
DIY incubator
Temperature can be set to ~40℃ by
reducing the AC input voltage to 30V
from normal ~100V.
The blueprint of DIY incubator is on
GitHub. Both are at ~$100.
by @okgw_
Household fan centrifuge (~100G at ~1000rpm)
Egg white antimycotic and DIY clean bench
http://interestor2012.hatenablog.com/
entry/2015/01/05/202524
(@earthlyworld
88. Development of DIY cell culture protocols
Materials and methods are uploaded on blogs, video sharing
sites, GitHub etc. for other participants to confirm reproducibility.
Creative Cells Kyoto reproduces sports drink
culture medium experiment
https://cleanmeat-kyoto.hatenablog.com/
http://zacmayu.hatenablog.com/
http://www.nhk.or.jp/gendai/articles/4149/
Ms. Sugisaki - consultant in the day,
biohacker at home (aired on TV)
90. Development of home and school cell culture kit
Pre-survey to high school teachers
School cell
culture kit
should be
at <$300
DIY cell culture hardwares and
protocols deployed in a class
-At what budget?
-Cell culture in school classes?
(9 respondents)
◆<$300
◆<$500
◆<$1000
◆<$3000
◆<$5000
◆Yes
◆No
◆
◆
◆
Yes, as an extra-
curricular activity
Yes, if there is time
Depends on budget
@thesow41
(towel warmer)
(DIY protocols)
91. DIY cell culture and future
Other cell types?
Coculture system,
better DIY serum?
Cell scaffold, DIY
tissue engineering
….etc.
What’s
next?
DIY cell culture
experiments
Materials and methods
on blogs and online
videos
Cell culture kit
Results are shared,
other participants also
reproduce results
Cell culture protocols
Cell passage protocols,
cell library
92. Why stop at meat? DIY tissue engineering?
Tastier
than meat
DIY
kidney!
DIY differentiation
& morphogenesis
to make heart
Is this even
“meat”!?
“Green meat” algae-
meat composite food
https://www.ncbi.nlm.nih.gov/pmc/art
icles/PMC5282507/
・Bioreactors improve over time,
enabling cell, tissue & organ culture
・DIY tissue engineering is ubiquitous,
innovative prototypes everywhere
93. Local farmers design their own meat brands
・Local farmers and town butchers
can develop their own cell-based
meat recipe.
・Brand ownership opportunity
opens up for local farmers.
・Hobbyists come up with unique
meat recipe.
94. Beef branded on individual cows
Cell source
cow still alive
Scan the QR code
printed on package
See how the
cow is doing
102. Creative support for artists
“I deal with Ideal Meal” by Yamada Theta
https://twitter.com/yamada_theta
https://www.pixiv.net/member_illust.php?mode=medium&illust_id=66586574
https://note.mu/yamada_theta/n/n243f40556409
《Schroedinger’s Tiger》 by Michi Okada
http://tobira-project.info/blog/2017_okada.html
http://michiokada.tumblr.com/
106. Visual novel source files
Source files
https://drive.google.com/drive/u/0/folders/0B0ShPzNziL05THlwU2IwTTNueHM
(Feel free to produce alternative scenarios, derivative works, add
characters, etc. under CC-BY-NC license.)
“Miyo-san” and “Aco-chan” whole body and face PNG images and
sci-fi themed background images available for manga and VN’s. MiyoAco
109. Zine's (DIY cell culture manuals)
2017.08
Intro book
2017.08
Recipe book
2017.12
Cultured foie gras
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Distributed at Comic Market, COMITIA, TechBookFest, etc. Also available from ComicZIN
2016.08
First edition
2016.12
“Made & ate”
Sold out
110. Members participate in their respective expertise (experiments,
gatherings, art projects etc.) - no defined “membership”
#Food Security
#Food Miles
#Regulations
#Cooking
#Culture & thoughts
#History
#Food safety
#Life ethics
#Animal welfare
#Regenerative medicine
#Tissue engineering
#Bioreactor
#Culture medium
#Commercialization
#LCA
#R&D
#Soc.&Econ.
#Global collab.
#Space
#Art
Shojinmeat Project “Distributed Clusters”
All welcome:
Please join from Shojinmeat Project English Slack Channel
https://medium.com/@jaysonvdw/announcement-english-slack-channel-for-the-shojinmeat-project-a8
00e3886799
111. Some past questions (part 1)
Q: I would like to participate
A: There is no formal definition of membership.
Please Tweet to us or join slack channel.
Q: I’m not a biologist but would like to join
A: All disciplines welcome - multiple journalism,
art & other non-sci/tech projects are ongoing
Q: Where are carbon and nitrogen sourced?
A: Culture medium contains sugar and amino
acids. Amino acid is sourced from yeast which
feed on sugar. In future, artificial photosynthesis
and nitrogen fixation may take this role.
Q: Is fungal farming cellular agriculture?
A: Depends on if cell culture procedure is
involved in the process. Cultivation of entire
fungal body would be conventional farming.
Q: Is cell-based meat GMO?
A: Gene editigin is not required for cell culture. In
future, “designer meat” such as “allergen-free
meat” may require gene editing.
Q: Is it tasty?
A: It’s pasty (for now). In future, advanced tissue
engineering may enable complete reconstruction
of meat taste and texture and even go beyond.
Q: How quickly would “meat” grow?
A: Cells multiply every 1½~2 days. 1E5 cells
grow to visible size in 20~30 days. Flow process
in factories may enable continuous production.
Q: What will happen to farm animals?
A: They don’t disappear as starter cells would
still be required, but their number may decline.
112. Some past questions (part 2)
Q: Could meat be cocultured with probiotics?
A: Unknown. It depends on their growth rates and
mutual effect of cell metabolites.
Q: Why would people eat cell-based meat?
A: Initially, vegans and environmentally-conscious
would consume, but at the end, taste, price and
convenience would decide.
Q: My kids should try DIY cell culture
A: Hardware and methods are open for public.
Please contact us for details.
Q: Desktop clean bench may help?
A: Egg white can suppress mould growth, but a
clean bench always help.
Q: What will happen to farmers?
A: Farmers may gain an opportunity to build a
brand around his/her cow cell and meat culture
recipe, but it depends on what business model
cellular agriculture companies take in the future.
Q: Is cell-based meat Halal/Kosher?
A: It may potentially become Halal/Kosher under
certain conditions, according to a publication:
https://www.ncbi.nlm.nih.gov/pubmed/28456853
Q: Is it vegan?
A: Depends on technological maturity. Our DIY
procedure uses egg white as antimycotic and
therefore not vegan. Even in future, it would
depend on the exact procedure of cell acquisition.
113. Contacts for businesses
“Shojinmeat Project” is a citizen science community.
For formal consultation, IP license, joint R&D and other business
affairs, please contact Integriculture Inc. , a spin-off company
from Shojinmeat Project.
http://integriculture.jp