The Cell-Ag World 2020.02

Cell-based meat (cultured meat)
Muscle cells Bioreactor
Culture
medium
Processing

Myoblast grown
at 200t scale
Looks like a
beer factory?
Future: Meat brewery

Steak brewery
Single use
culture bags⇒
Steak grows inside⇒
Ready to ship⇒

Seafood by cellular agriculture
Cell culture at
room temperature
Small ponds as cell source

Culture media
produced by
microalgae
Short food miles
Farm high-rise
Cell-ag
towers
-95% water use,
-98% land use?
Tuomisto 2011, Oxford

Farmscape, A.D.2203
Vertical farms
Vast land
reverted to
nature
Artificial photosynthesis
Cellular agriculture manu-
facturing industry complex

Martian food production facility

The technical problem
$325,000
Cell-based burger, 200g

Technological milestones
Scaling & automation
Inexpensive media
Openness & public trust
Appealing products

Cell-Ag movement and spin-offs
Citizen Science
For-profit Startup
Non-Profit
2014 2015 2017 2019

“Open Source Cultured Meat”
Growing meat at home
Schoolkids culture
cells in kitchens
Manuals in Comiket
Airtight box
CO2 source humidity source
dish

“Food grade” demo - Sports drink culture media
90% DAKARA
80%
70%
60%
0%
(DMEM only)
50%
DaysCelldivisions
Proliferation of mouse L6 in DMEM/GreenDakara 10% FBS
#pH and osmolality of Dakara adjusted by NaHCO3(s) and 2M NaOH
Fluid name osmolality pH
DMEM(-, hi glu) 345 7.4
Pocari 338 3.4
Aquarius 291 3.37
Amino-Value 4000 289 3.63
AminoVital Gold 186 3.33
Vitamin Water 302 3.3
Green DAKARA 322 3.28
Amiiru Water 249 3.4
Mamoru Chikara 546 3.58

“CulNet System” proof of concept
▲PoC #1 and #2 ▼Automated PoC#3 2018～
2016 2017
Cultured avian
liver paste
Cultured
muscle tissue

R&D roadmap
Functional
compound
s, i.e.
cosmetics
Pasty foods,
i.e. foie gras
Processed
meat
salami
Food
Medium
Serum BloodHormone
Vascular
ization
2020 2021 2023
Tissues,
i.e. steak
2025
R&D
JP Pat. filed
2018-210910
“Space Salt”
end of 2019
2019

The road ahead in protein transformation
Meat by locals,
democratization
Hype cycle
Price parity reached
...by categories & regions
Meat from complexes
2020～ 2030～Animal meat
Cell-based
meat
Price
Time
Foie gras Beef Chicken
Importer Half-producer Producer

Less cows
Bioreactors in
the backyard
Many cows
Cows are the product Cells or meat is the product
Traditional farmscape Cell-ag farmscape
The Cell-Ag Farmscape
Less feeding, cleaning and waste treatment
More facility management as in beer brewery

“Star Cow Talent Agency”
・Brands by
individuals, not
breed lines
・Animal talent
agencies
Talent agency
Cell-based meat
Brand
development
Food
companies

Cost of DNA synthesis Cost of cell culture
“Write DNA & grow cells”
DIY insulin for those
who can’t afford
http://openinsulin.org/
Concept demonstrated
DIY cell culture

Pharma
company DIY gene edit
Cheap DNA
DIY cell culture,
manufacture
drugs at home
”Drug DNA leaked to 4chan”

Capybara Electric eel Yellow fish
Design an
animal like
LEGO
Designed animal &
its meat from
home culture vat
A fictional electric rat for pet

Prototyping of alien biospheres
Avatar (2009),
Forest of Pandora

Perhaps, someday in the future on Mars….