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Salmonella Essential learning for Medical Students
Salmonella is a bacteria that causes typhoid fever and food poisoning in humans. Typhoid fever, caused by Salmonella typhi, results in a sustained high fever as the bacteria spreads from the intestines to other organs. It may cause complications like intestinal perforation or hemorrhage. The early symptoms of typhoid fever include poor appetite, headaches, fever up to 104 degrees Fahrenheit, and lethargy. Left untreated, 5-10% of typhoid cases may experience relapse of symptoms.
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Salmonella Essential learning for Medical Students
- 1. Salmonella Essential learning forMedical Students Dr.T.V.Rao MD Dr.T.V.Rao MD 1
- 2. Salmonella • Causes Infectionsin Humans and vertebrates, • Enteric Fever ( Typhoid fever ) • Gastroenteritis • Septicemias, • Carrier state a concern Dr.T.V.Rao MD 2
- 3. Salmonella • A Verycomplex group • Contains more > 2,500 spp • Typed on the basis of Serotyping, and species typing • Divided into two groups 1 Enteric fever group 2 Food poisoning group – 3 Septicemias Dr.T.V.Rao MD 3
- 4. Key points • Thereare more than 2500 different antigenic types of Salmonella; those pathogenic to man are serotypes of S. enterica. • Most serotypes of S. enterica cause food- borne gastroenteritis and have animal reservoirs. • S. enterica serotypes Typhi and Paratyphi cause typhoid fever. Dr.T.V.Rao MD 4
- 5. Enteric Fever Typhoid Fever •Caused by Salmonella typhi, and other Groups called as Paratyphoid A, B, C • Salmonella typhi - Causes Typhoid • Salmonella Paratyphi A,B,C Causes Paratyphoid fevers. • Food Poison group • Spread from Animals – Humans • Causes Gastroenteritis – Septicemias, Localized Infection Dr.T.V.Rao MD 5
- 6. Salmonella typhi • Thebacterium Salmonella typhi is present only in human beings and is transmitted through contaminated food or water. People with this infection carry the bacterium in their intestines and bloodstream, and those who have recovered from the disease could still have the bacterium in their system; they are known as ‘carriers’ of the disease. Both ill people and carriers shed Salmonella typhi in their stool Dr.T.V.Rao MD 6
- 7. Food handlers spreadthe infection • Both ill people and carriers shed Salmonella typhi in their stool • Infection is usually spread when food or water is handled by a person who is shedding the bacterium or if sewage water leaks into drinking water or food that is then consumed.. Dr.T.V.Rao MD 7
- 8. Typhoid fevers areprevalent in many regions in the World
- 9. Typhoid Mary MostDangerous Woman in America Dr.T.V.Rao MD 9
- 10. Typhoid Mary • Afamous example is “Typhoid” Mary Mallon, who was a food handler responsible for infecting at least 78 people, killing 5. These highly infectious carriers pose a great risk to
- 11. Typhoid Mary • "TyphoidMary," real name Mary Mallon, worked as a cook in New York City in the early 1900s. Public health pioneer Sara Josephine Baker, MD, PhD tracked her down after discovering that she was the common link among many people who had become ill from typhoid fever She was traced to typhoid outbreaks a second time so she was put in prison again where she lived until she died.
- 12. French physician PierreCharles Alexandre Louis first proposed the name “typhoid fever” William Wood Gerhard who was the first to differentiate clearly between typhus fever and typhoid in 1837.
- 13. Carl Joseph Eberthwho discovered the typhoid bacillus in 1880. Georges Widal who described the ‘Widal agglutination reaction’ of the blood in 1896.
- 14. Morphology of Salmonella •Gram negative bacilli • 1-3 / 0.5 microns, • Motile by peritrichous flagella Dr.T.V.Rao MD 14
- 15.
- 16. Bacteriology –Typhoid fever •The Genus Salmonella belong to Enterobacteriaceae • Facultative anaerobe • Gram negative bacilli • Distinguished from other bacteria by Biochemical and antigen structure Dr.T.V.Rao MD 16
- 17. Different types ofSalmonella I - enterica II - salamae IIIa -arizonae IIIb -diarizonae IV - houtenae V - bongori VI - indica
- 18. Cultural Characters • Aerobic/ Facultatively anaerobic • Grows on simple media – Nutrient agar, • Temp 15 – 41ºc / 37º c • Colonies appear as large 2 -3 mm, circular, low convex, • On MacConkey medium appear Colorless ( NLF ) Selective Medium - Wilson Blair Bismuth sulphide medium. Produce Jet black colonies H2 S produced by Salmonella typhi Dr.T.V.Rao MD 18
- 19. Enrichment Medium Liquid Medium •Selenite F medium • Tetrathionate broth • Above medium are used for isolation of Salmonella from contaminated specimens • Particularly stool specimens.. Dr.T.V.Rao MD 19
- 20. Identifying Enteric Organisms •Isolates which are Non lactose fermenting • Motile, Indole negative • Urease negative • Ferment Glucose,Mannitol,Maltose • Do not ferment Lactose, Sucrose • Typhoid bacilli are anaerogenic • Some of the Paratyphoid form acid and gas • Further identification done by slide agglutination tests Dr.T.V.Rao MD 20
- 21. Biochemical Characters • Glucose,Mannitol ,Maltose produce A/G • Salmonella typhi do not produce gas • Lactose/Salicin/sucrose not fermented. • Indole – • Methyl Red + • V P - • Citrate + • Urea – • H2S – produced by Salmonella typhi • Paratyphi A do not produce H2S Dr.T.V.Rao MD 21
- 22. Resistance of Salmonella •55º c – 1 hour • 60º c – 15 MT • Boiling ,Chlorination, Pasteurization Destroy the Bacilli. Dr.T.V.Rao MD 22
- 23. Salmonella Antigenic Structure • H– Flagellar antigens • O – Somatic antigen, • Vi – Surface antigen in some species only • H antigens also called flagellar antigens, heat labile protein, • Boiling destroys antigenicity • When mixed with Antiserum produces agglutination and fluffy clumps are produced • H antigens are strongly immunogenic Induces antibodies rapidly, Dr.T.V.Rao MD 23
- 24. Antigenic structure ofSalmonella • Two sets of antigens • Detection by serotyping • 1 Somatic or 0 Antigens contain long chain polysaccharides ( LPS ) comprises of heat stable polysaccharide commonly. • Dr.T.V.Rao MD 24
- 25. Flagellar Antigens • Flagellaror H Antigens are strongly immunogenic and induces antibody formation rapidly and in high titers following infection or immunization. The flagellar antigen is of a dual nature, occurring in one of the two phases. • Dr.T.V.Rao MD 25
- 26. Antigens – Salmonella( cont ) Dr.T.V.Rao MD 26 • O Antigens • Forms integral part of Cell wall, • Like Endotoxin • 0 Antigens unaffected by boiling. • When mixed with antiserum produce chalky clumps are formed, take more time reaction, at high temp 50º – 55º c • O antigens are less immunogenic. than H antigens
- 27. Antigen (Vi) –Salmonella ( contd ) • Vi antigens • Many strains in S.typhi covers the O antigens- prevents agglutination. • Resembles like K antigens • Destroyed after boiling at 60º c / 1 hour. • Vi a polysaccharide • Acts as virulence factor, protects the bacilli against Phagocytosis and activity of Complement • Poorly immunogenic • Low titer of antibodies are produced, Not diagnostic Dr.T.V.Rao MD 27
- 28. Classification of Salmonella •Classified on the basis of Kauffmann-White Scheme • Structure of 0 and H antigens are taken into consideration, • More than 2000 species characterized. Dr.T.V.Rao MD 28
- 29. Kauffmann – Whitescheme • Serotype 0 antigens H antigens Phase 1 2 1.Typhi 9,12,(Vi) d 1,2 2 Paratyphi A 1,2.12 a - 3 Paratyphi B 1,4,5,12 b 1,2 4 Typhimuruim 1,4,5,12 I 1,7 5 Enteritidis 1,9,12 g m 1,2 Dr.T.V.Rao MD 29
- 30. Antigenic Variation in Salmonella •May be phenotypic / Genotypic • H to O = loss of Flagella May be phase variation from I to II V to W variation S to R variation Dr.T.V.Rao MD 30
- 31. Pathogenicity • Salmonella aredefinite parasites to humans. • Eg S.typhi. • S.paratyphi A, B ,C • Other groups Salmonella • The important clinical syndromes 1. Enteric fever, Septicemias, gastroenteritis. Dr.T.V.Rao MD 31
- 32. Enteric Fever: S.typhi • Ileocecal penetration • intraluminal multiplication • mononuclear response (macrophages) • Salmonella remains alive • 2nd week - lymphoid hyperplasia (mesenteric lymph nodes) • back to bowel
- 33. Enteric Fever Typhoid • Typhoid– caused by S.typhi • Paratyphoid Caused by Paratyphi A,B,C • Typhoid --- Like Typhus • Infective dose ID50 / 107, Dr.T.V.Rao MD 33
- 34. Fever • All theevents coincides with Fever and other signs of clinical illness • From Gall bladder further invasion occurs in intestines • Involvement of peyr’s patches, gut lymphoid tissue • Lead to inflammatory reaction, and infiltration with monocular cells • Leads to Necrosis, Sloughing and formation of chacterstic typhoid ulcers
- 35.
- 36. Rashes in Typhoid •May present with rash, rose spots 2 -4 mm in diameter raised discrete irregular blanching pink maculae's found in front of chest • Appear in crops of upto a dozen at a time • Fade after 3 – 4 days
- 37.
- 38. Events in aTypical typhoid Fever Dr.T.V.Rao MD 38
- 39. Pathology and Pathogenesis •Bacilli enter through ingestion, • Bacilli attach to Microvilli,ileal mucosa, penetrate to Lamina propria and sub mucosa • Phagocytosis by Polymorphs and Macrophages • Enters the mesenteric lymph nodes • Enter the thoracic duct – Blood stream Dr.T.V.Rao MD 39
- 40. Infective Dose • Forhuman infections, the number of bacteria that must be swallowed in order to cause infection is uncertain and varies with the serotype. In most of these the median infective dose for most serotypes, including Typhi, has varied from 106 to 109 viable organisms. Dr.T.V.Rao MD 40
- 41. Pathology and Pathogenesis •Bacteremia Spread to Liver, Gall bladder, Spleen, Bone marrow, Lymph nodes, Lungs, Multiply in kidneys Once again spill into Blood stream Causes clinical illness. Dr.T.V.Rao MD 41
- 42. Ingestion of contaminatedfood or water Salmonella bacteria Invade small intestine and enter the bloodstream Carried by white blood cells in the liver, spleen, and bone marrow Multiply and reenter the bloodstream
- 43. Pathology and Pathogenesis •Multiply abundantly in Gall bladder, • Bile rich source of Bacteria • Spill into Intestine, infects payers patches, Lymph follicles • Inflammation – Undergo necrosis, Slough off • Typhoid ulcers • Typhoid ulcers can cause perforation and hemorrhage • Duration of Illness 3 – 4 weeks • Incubation 7 -14, ( 3-56 days ) Dr.T.V.Rao MD 43
- 44. What is EntericFever Typhoid Fever • Enteric fever is caused by strains of S. Typhi or S. Paratyphi A, B or C; although S. Paratyphi B, which gene sequence analysis suggests is a variant of S. Java, is more likely to cause non-typhoidal diarrhoea. Dr.T.V.Rao MD 44
- 45. S.typhi more serious •The clinical features tend to be more severe with S. Typhi (typhoid fever). After penetration of the ileal mucosa the organisms pass via the lymphatic's to the mesenteric lymph nodes, whence after a period of multiplication they invade the bloodstream via the thoracic duct. Dr.T.V.Rao MD 45
- 46. Manifest with Rose spots Highfever Diarrhea Typhoid Meningitis Aches and pains Chest congestion
- 47. Symptoms • No symptoms- if only a mild exposure; some people become "carriers" of typhoid. • Poor appetite, • Headaches, • Generalized aches and pains, • Fever, Lethargy, Lethargy, • Lethargy, • Diarrhea, • Have a sustained fever as high as 103 to 104 degrees Fahrenheit (39 to 40 degrees Celsius), • Chest congestion develops in many patients, and abdominal pain and discomfort are common, • Constipation, mild vomiting, slow heartbeat.
- 48. Progress in EntericFever • The liver, gall bladder, spleen, kidney and bone marrow become infected during this primary bacteraemic phase in the first 7-10 days of the incubation period. After multiplication in these organs, bacilli pass into the blood, causing a second and heavier bacteraemia, the onset of which approximately coincides with that of fever and other signs of clinical illness. Dr.T.V.Rao MD 48
- 49. Progress in EntericFever • From the gall bladder, a further invasion of the intestine results. Peyer's patches and other gut lymphoid tissues become involved in an inflammatory reaction, and infiltration with mononuclear cells, followed by necrosis, sloughing and the formation of characteristic typhoid ulcers occurs. Dr.T.V.Rao MD 49
- 50. Immunity in Typhoid •Typhoid bacilli are Intracellular pathogens • Cell mediated immunity is crucial Dr.T.V.Rao MD 50
- 51. Early symptoms ofTyphoid Fever • The incubation period is usually 1-2 weeks, and the duration of the illness is about 3-4 weeks. Symptoms include: • Poor appetite • Headaches • Generalized aches and pains • Fever as high as 104 degrees Farenheit • Lethargy • Diarrhea Dr.T.V.Rao MD 51
- 52. Diarrhea Nausea Vomiting Stomach pain Headache Fever Onset 12-72 hours after infection
- 53. Clinical manifestation • Headache, malise,anorexia ,coated tongue • Abdominal discomfort, • Constipation / Diarrhea • Step ladder type fever, • Relative bradycardia, • A soft palpable spleen • Hepatomegaly • Rose spots appear Dr.T.V.Rao MD 53
- 54. Events in aTypical typhoid Fever Dr.T.V.Rao MD 54
- 55. Complications of Entericfever • Intestinal perforation, • Hemorrhage, • Circulatory collapse. • Bronchitis Bronchopneumonia, • Meningitis, • Cholecystitis, • Arthritis,Periostitis / Nephritis, • Osteomyletis, Dr.T.V.Rao MD 55
- 56. Relapses in TyphoidFever • Apparent recovery can be followed by relapse in 5-10% of untreated cases. Relapse is usually shorter and of milder character than the initial illness, but can be severe and may be fatal. Severe intestinal haemorrhage and intestinal perforation are serious complications that can occur at any stage of the illness. Dr.T.V.Rao MD 56
- 57. Other complications • Causesrelapses in particular to patients treated with chloramphenicol. • S.paratyphi produce septicemias. Dr.T.V.Rao MD 57
- 58. Typhoid carriers • Salmonellaenterica causes approximately 16 million cases of typhoid fever worldwide, killing around 500,000 per year. One in thirty of the survivors, however, become carriers. In carriers the bacteria remain hidden inside cells and the gall bladder, causing new infections as they are shed from an apparently healthy host.
- 59. Carriers may be Carriersmay be temporary or chronic. Temporary (convalescent or incubatory) carriers usually excrete bacilli up to 6-8 weeks. By the end of one year, 3-4 per cent of cases continue to excrete typhoid bacilli. Persons who excrete the bacilli for more than a year after a clinical attack are called chronic carriers.
- 60. Carrier Stage inTyphoid Fever • Most people infected with salmonella continue to excrete the organism in their stools for days or weeks after complete clinical recovery, but eventual clearance of the bacteria from the body is usual. A few patients continue to excrete the salmonellae for prolonged periods. The term chronic carrier is reserved for those who excrete salmonellae for a year or more. Dr.T.V.Rao MD 60
- 61. Carrier Stage inTyphoid Fever • Chronic carriage can follow symptomatic illness or may be the only manifestation of infection. It can occur with any serotype, but is a particularly important feature of enteric fever: up to 5% of convalescents from typhoid and a smaller number of those who have recovered from paratyphoid fever become chronic carriers, many for a lifetime. Dr.T.V.Rao MD 61
- 62. Carrier Stage inTyphoid Fever • The bacilli are most commonly present in the gall bladder, less often in the urinary tract, and are shed in faeces and sometimes in urine. The long duration of the carrier state enables the enteric fever bacilli to survive in the community in non-epidemic times and to persist in small and relatively isolated communities. Dr.T.V.Rao MD 62
- 63. Epidemiology • Developed countries- Controlled. • Water supply/ Sanitation /Economically poor. • S.typhi and S.paratyphi are prevalent in India • Previously Typhi are more common Paratyphoid A on raise. • Age 5 – 20 years, Sanitation Dr.T.V.Rao MD 63
- 64. Epidemiology • Sanitation hasgreat role • Source an active patient or a Carrier shed the Bacilli. • Who are carriers. Convalescent carrier 3 weeks to 3 months Temporary carrier 3 months to 1 year Chronic carrier > 1 year, Women attain more carrier stage Dr.T.V.Rao MD 64
- 65. Epidemiology (Contd) • Bacillipersist in the Gall bladder and kidney • Food handlers spread the infection • Cooks great role • S.typhi and S.paratyphi in humans • S.para B in Animals, • Typhoid spread through Water, Milk, Food HIV patients potentially susceptible for Typhoid disease. Dr.T.V.Rao MD 65
- 66.
- 67. Bacteriological Diagnosis ofTyphoid Fever • Selective media, such as Deoxycholate- citrate agar or xylose-lysine Deoxycholate agar, are used for the isolation of salmonella bacteria from faeces. Fluid enrichment media, such as Tetrathionate or selenite broth, are also useful to detect small numbers of salmonellae in faeces, foods or environmental samples. Dr.T.V.Rao MD 67
- 68. Bacteriological Diagnosis of TyphoidFever • Suspicious colonies from the culture plates are tested directly for the presence of Salmonella somatic (O) antigens by slide agglutination and subcultured to peptone water for the determination of flagellar (H) antigen structure and further biochemical analysis. Dr.T.V.Rao MD 68
- 69. Bacteriological Diagnosis of TyphoidFever • A presumptive diagnosis of salmonellosis can often be made within 24 h of the receipt of a specimen, although confirmation may take another day, and formal identification of the serotype takes several more days. A negative report must await the result of enrichment cultures - at least 48 h. Dr.T.V.Rao MD 69
- 70. How we DiagnoseTyphoid Fever • Diagnosis is made by any blood, bone marrow or stool cultures and with the Widal test (demonstration of salmonella antibodies against antigens O-somatic and H-flagellar ). In epidemics and less wealthy countries, after excluding malaria, dysentery or pneumonia, a therapeutic trial time with chloramphenicol is generally undertaken while awaiting the results of Widal test and cultures of the blood and stool. Dr.T.V.Rao MD 70
- 71. Laboratory Diagnosis of TyphoidFever • 1 Isolation of Bacilli. A Gold standard • 2 Diagnosis for presence of Antibodies, • Positive Blood culture – A gold standard • Isolation from Feces and Urine ? • Detection of Antibodies Inconclusive. • Newer methods Detection of antigen in Blood and Urine Dr.T.V.Rao MD 71
- 72. Blood Culture 1 stweek Positive in 90 % 2 nd week Positive in 75 % 3 rd week Positive in 60 % > 3 weeks positive in 25 % Draw 5 – 10 cc of Blood by venipuncture. ADD to 50 -100 ml of Bile broth. Incubate at 37 c /Subculture in MacConkey At regular intervals Dr.T.V.Rao MD 72
- 73. Blood Cultures inTyphoid Fevers • Bacteremia occurs early in the disease • Blood Cultures are positive in 1st week in 90% 2nd week in 75% 3rd week in 60% 4th week and later in 25% Dr.T.V.Rao MD 73
- 74. Castaneda’s method of BloodCulture • Double medium used Solid/Liquid medium in the same Bottle. • Bottle contains Bile broth/agar slant, • For subculture the bottle is merely tilted. • A subculture into MacConkey at regular intervals, • Reduces the chances of contamination • Increases the chances of isolation. Dr.T.V.Rao MD 74
- 75. Salmonella on MacConkey's agar Dr.T.V.Rao MD 75
- 76. Salmonella on XLDagar Dr.T.V.Rao MD 76
- 77. Clot culture • Clotcultures are more productive in yielding better results in isolation. • A blood after clotting, the clot is lysed with Streptokinase ,but expensive to perform in developing countries. Dr.T.V.Rao MD 77
- 78. Bactec and Radiometricbased methods are in recent use • Bactek methods in isolation of Salmonella is a rapid and sensitive method in early diagnosis of Enteric fever. • Many Microbiology Diagnostic Laboratories are upgrading to Bactek methods Dr.T.V.Rao MD 78
- 79. Biochemical Characters • NonLactose fermenter, • Motile • Indole – MR + VP - Citrate + • Ferment Glu/Mal/Man • Do not ferment Lactose/Sucrose Dr.T.V.Rao MD 79
- 80. Slide agglutination tests •In slide agglutination tests a known serum and unknown culture isolate is mixed, clumping occurs within few minutes • Commercial sera are available for detection of A, B,C1,C2,D, and E. Dr.T.V.Rao MD 80
- 81. Culturing other Specimens •Feces Enrichment in Tetrathionate broth and Selenite broth • Culturing in MacConkey/DCA/Wilson Blair medium – Large black colonies. • Urine Culture – positive in 25 % • Other samples Bone Marrow,Bile,CSF/Sputum Dr.T.V.Rao MD 81
- 82. Serology • WIDAL Test– Tube agglutination test. • Detects O and H antibodies • Diagnosis of Typhoid and Paratyphoid • Testing for H agglutinins in Dryers tubes, a narrow tube floccules at the bottom • Testing for O agglutinins in Felix tubes, Chalky • Incubated at 37º c overnight Dr.T.V.Rao MD 82
- 83. Widal Test • In1896 Widal A professor of pathology and internal medicine at the University of Paris (1911–29), he developed a procedure for diagnosing typhoid fever based on the fact that antibodies in the blood of an infected individual cause the bacteria to bind together into clumps (the Widal reaction). Dr.T.V.Rao MD 83
- 84. Widal test • S.typhiO and H tubes • Paratyphi A/B H agglutinins only • Common antigens O in all Factor sharing 12 • Significance • I st week negative. • Titers raise in 2nd week Raise of titers diagnostic Dr.T.V.Rao MD 84
- 85. Diagnosis of EntericFever Widal test • Serum agglutinins raise abruptly during the 2nd or 3rd week • The Widal test detects antibodies against O and H antigens • Two serum specimens obtained at intervals of 7 – 10 days to read the raise of antibodies. • Serial dilutions on unknown sera are tested against the antigens for respective Salmonella • False positives and False negative limits the utility of the test • The interpretative criteria when single serum specimens are tested vary • Cross reactions limits the specificity
- 86. Widal Test • Singletest not diagnostic. • Paired samples tests • Diagnostic. O > 1 in 80 H > 1in 160 H agglutinins appear first False positives in Unapparent infection, Immunization Previously infected Dr.T.V.Rao MD 86
- 87. Widal test • Anamnesticresponse previous infection and responding to unrelated infection • Other Diagnostic tests CIE and ELISA Detection of Circulating antigens Co agglutination test. Dr.T.V.Rao MD 87
- 88. Limitation of WidalTest • The Widal test is time consuming and often times when diagnosis is reached it is too late to start an antibiotic regimen. • In spite of several limitation many Physicians depend on Widal Test Dr.T.V.Rao MD 88
- 89. False Positive andNegative Reactions with WIDAL Test • The Widal test should be interpreted in the light of baseline titers in a healthy local population. This is especially important when there is a high local prevalence of non-typhoid salmonellosis. The Widal test may be falsely positive in patients who have had previous vaccination or infection with S typhi. Dr.T.V.Rao MD 89
- 90. False Positive andNegative Reactions with WIDAL Test • Widal titers have also been reported in association with the dysgammaglobulinaemia of chronic active hepatitis and other autoimmune diseases.64 '8 '9 False negative results may be associated with early treatment, with "hidden organisms" in bone and joints, and with relapses of typhoid fever. Occasionally the infecting strains are poorly immunogenic. Dr.T.V.Rao MD 90
- 91. Diagnosis of Carriersand Environments • Fecal carriers by isolation from specimens. or Bile aspirated. • Sewer swabs • Bacteriophage typing Dr.T.V.Rao MD 91
- 92. Prophylaxis • TAB vaccine S.typhi1,000 millions S Paratyphi A,B 750 millions. Injected subcutaneously 0.5 ml at 4 – 6 weeks. Live Oral Vaccine Typhoral Mutant S.typhi strain Ty 2 1a Lacking enzyme UDP galctose 4 epimerase 10 to9 Viable bacilli Given orally 1 – 3 – 5 days Dr.T.V.Rao MD 92
- 93. Key points • Antibioticshave no place in the management of salmonella gastroenteritis unless invasive complications are suspected. • Clean water, sanitation and hygienic handling of foodstuffs are the keys to prevention. Dr.T.V.Rao MD 93
- 94. Prevention • Vi Polysaccharidevaccine – Administered subcutaneously or intramuscular – Confers protection seven days after injection – Approximately 50% efficacy after three years • Ty 21 vaccine – Live attenuated strain of S. typhi – Administered orally in capsule form – Also available in liquid form which can be taken by children as young as two years of age
- 95. Vaccines • An Injectable vaccine Typhium Vi • Contains purified Vi polysaccharide antigen from S.typhi strain Ty2 • A single dose, subcutaneous route • Given to children > 5 years • Immunity lasts for 2- 3 years. • Follow a booster Dr.T.V.Rao MD 95
- 96. Treatment • Chloramphenicol 1948/1970 resistance. • Other Important drugs Ampicillin Amoxicillin, Furazolidine Cotromoxazole Chloramphenical resistance /Mexico Kerala Dr.T.V.Rao MD 96
- 97. Antimicrobial Therapy inTyphoid • With prompt antibiotic therapy, more than 99% of the people with typhoid fever are cured, although convalescence may last several months. The antibiotic chloramphenicol Some Trade Names CHLOROMYCETIN is used worldwide, but increasing resistance to it has prompted the use of other antibiotics BACTRIM SEPTRAN or ciprofloxacin
- 98. Other Drugs • Fluroquinolones Ciprofloxacillin, Pefloxacillin Ofloxacillin Ceftazidime Ceftriaxone/ Cefotoxaime Dr.T.V.Rao MD 98
- 99. Coalition against Typhoid •Since May 2011, the Coalition against Typhoid (CaT) has featured monthly articles in the WHO’s Global Immunization Newsletters (GIN). The articles, written by CaT members from around the world, highlight important work being done to accelerate adoption of typhoid vaccines. Dr.T.V.Rao MD 99
- 100. Salmonella • WHO: 1,400,000instances of salmonellosis in USA • Salmonella costs per year US $3,000,000,000 • 2,300 serotypes (S. enterica Typhimurium, etc) – wide host range (humans, cattle, horses, rodents, cats, dogs, birds, reptiles) • Multi-drug resistant S.e.Typhimurium DT104
- 101. Salmonella • Most commondiseases caused by Salmonella: • gastroenteritis (self-limiting, 2-5 days) • enteric/typhoid fever (incubation 1-10/7-14 days, lasts 2-3 wks) • septicemia (incubation12-36 hrs, may lead to chronic infection) • symptoms and disease manifestation differ in hosts 101
- 102. Food Poisoning • Thelaboratory diagnosis of bacterial food poisoning depends on isolation of the causal organism from samples of faeces or suspected foodstuffs. The more common food-poisoning serotypes, such as Enteritidis or Typhimuruim, may be characterized more fully by phage typing and antibiotic resistance typing (see above). Dr.T.V.Rao MD 102
- 103. Salmonellosis • > 2000known serotypes • 200 serotypes are detected each year in the United States • Two most common (in most countries) – Salmonella Typhimurium – Salmonella Enteritidis
- 104. Identification of pathogens •Strains can be differentiated further by plasmid and pulsed- field gel electrophoresis typing so that the isolates from patients may be matched with those from the infected food and from a suspected animal source. Dr.T.V.Rao MD 104
- 105. Salmonella Gastroenteritis • Zoonoticdisease • S.enteritidis • S.typhimurium • S.halder • S. agana • S.indiana • Contaminated poultry, Meat Milk, Milk products. • Enters the shells of the Intact eggs – Chicken feed, and Fecal droppings. Dr.T.V.Rao MD 105
- 106. Nontyphoidal Salmonella • GeneralIncubation: 6 hrs-10 days; Duration: 2-7 days • Infective Dose = usually millions to billions of cells • Transmission occurs via contaminated food and water • Reservoir: a) multiple animal reservoirs b) mainly from poultry and eggs (80% cases from eggs) c) fresh produce and exotic pets are also a source of contamination (> 90% of reptile stool contain salmonella bacterium); small turtles ban. • General Symptoms: diarrhea with fever, abdominal cramps, nausea and sometimes vomiting Dr.T.V.Rao MD 106
- 107. Mechanism of Pathogenicity Gastroenteritis •ingestion • absorbed to brush border of epithelial cells of small intestine and colon • migrate to lamina propria, Ileocecal • multiply in lymphoid follicles • Reticuloendothelial hyperplasia and hypertrophy
- 108. Nontyphoidal Salmonella: Gastroenteritis • Incubation:8-48 hrs ; Duration: 3-7 days for diarrhea & 72 hrs. for fever • Inoculum: large • Limited to GI tract • Symptoms include: diarrhea, nausea, abdominal cramps and fevers of 100.5-102.2ºF. Also accompanied by loose, bloody stool; Pseudo appendicitis (rare) • Stool culture will remain positive for 4-5 weeks • < 1% will become carriers Dr.T.V.Rao MD 108
- 109. Nontyphoidal Salmonella: Bacteremia andEndovascular Infections • 5% develop septicemia; 5-10% of septicemia patients develop localized infections • Endocarditis: Salmonella often infect vascular sites; preexisting heart valve disease risk factor • Arteritis: Elderly patients with a history of back/chest + prolonged fever or abdominal pain proceeding gastroenteritis are particularly at risk. - Both are rare, but can cause complications that may lead to death Dr.T.V.Rao MD 109
- 110. Salmonella Gastroenteritis • Canoccur as cross infection • 24 hours • Manifest with Diarrhea, omitting • Abdominal pain mucous and blood in stools • Last for 2 – 4 days • Some times may lead to septicemias Dr.T.V.Rao MD 110
- 111. Diagnosis and Treatment •Isolation by culturing • Rarely need antibiotics. • More frequent in Developed nations. Dr.T.V.Rao MD 111
- 112. Salmonella septicemias • S.cholerasuis • Deep abscess, Endocarditis • Isolation from Blood and Pus. • Chloramphenicol highly effective Dr.T.V.Rao MD 112
- 113. Don’t eatraw or undercooked food Cross-contamination of foods should be avoided Do not prepare food or pour water if you are infected with the bacteria
- 114. Wash hands,kitchen surfaces, and utensils with soap and water after they have come in contact with raw meat or poultry Wash hands after contact with animal feces Avoid direct/indirect contact between reptiles and infants
- 115. Simple hand hygieneand washing can reduce several cases of Typhoid
- 116. • Program createdby Dr.T.V.Rao MD for benefit of Medical and Paramedical Students in the Developing World • Email • doctortvrao@gmail.com Dr.T.V.Rao MD 116