Post fumigation
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This document discusses post-fumigation procedures for checking the effectiveness of laboratory fumigation. It outlines the steps to perform an experiment using agar plates to check for any remaining microbial contamination. The plates are incubated in the fumigated lab area for 24-36 hours. Colonies are then counted using colony forming units (CFU) to quantify the microbial load. If growth is limited, the results are positive and the lab is ready for aseptic work. However, more than limited growth would be negative, requiring repeated fumigation to eliminate remaining contaminants. Precautions like wearing protective equipment and sterilizing glassware are also described.
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The cannabis industry is rapidly growing in Canada, the world industry leader, and there is a lack of skilled workers in the cannabis space. According to the research performed by International Medical Cannabis Association in 2019, many growing cannabis Licensed Producers and related companies are struggling to find skilled workers in the space. Many individuals have a growing experience, but most do not have the appropriate education, credentials, and regulatory updates to be genuinely successful in the cannabis industry. Therefore, educational training is an essential and critical element in the cannabis space.
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留信网认证的作用:
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一.毕业证【q微741003700】成绩单、使馆认证、教育部认证、雅思托福成绩单、学生卡等！
二.真实使馆公证(即留学回国人员证明,不成功不收费)
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◇面对父母的压力，希望尽快拿到；
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4:这个认证书并且可以归档倒地方
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This is a short talk that I gave at the Banff International Research Station workshop on Modeling and Theory in Population Biology. The idea is to try to understand how the burden of natural selection relates to the amount of information that selection puts into the genome.
It's based on the first part of this research paper:
The cost of information acquisition by natural selection
Ryan Seamus McGee, Olivia Kosterlitz, Artem Kaznatcheev, Benjamin Kerr, Carl T. Bergstrom
bioRxiv 2022.07.02.498577; doi: https://doi.org/10.1101/2022.07.02.498577
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Post fumigation
- 1. PRESENTED BY SHIVANGI SHRIVASTAVA M.TECH, IV SEMESTER, SOBT, RGPV, BHOPAL Post-fumigation
- 2. Post fumigation is the process perform after fumigation for checking the contaminants are present or not inside the lab. Last and very important step of fumigation for potential aseptic environment of laboratory.
- 3. 1ST STEP AFTER FUMIGATION Wear PPE kit before going to the fumigated area. Check the plates in which chemicals are used for fumigation, in a way to avoid spillage. Discard the plates carefully in a Biosafety manner
- 4. Steps of post fumigation For checking the fumigation impact we should perform an experiment which shows the variation in microbial load For that preparing the agar plates and then incubated the petriplates in fumigated area After 24-36 hrs, colonies will appear on the plates For checking microbial load, colony should be count by CFU (Colony forming Unit)
- 5. POST FUMIGATION CONSEQUENCES Hinders the expected results of experiments Contaminants present can cause harmful effect on human health Increases hard work while performing experiments Waste of hazardous chemicals, euipments, media and money
- 6. EXPERIMENT TO CHECK THE CONTAMINATION Autoclave all the glasswares with media containing 3.8g Agar and 5 g LB Broth in 100ml distilled water at 121℃, 15 lbs for 15 min Pour the media into petri- dish in aseptic condition i.e. in laminar hood Placed the petriplates in the lab where fumigation was performed for 24-36 hrs.
- 7. COLONY COUNT CFU refer to the number of individual colonies of any microorganism that grow on a plate of media. This value in turn represents the number of bacteria capable of replicating as they have formed colonies on the plate. There is a CFU formula which involves sampling.
- 8. FOR FUMIGATION For bacterial interpretation Less than 10 bacterial colonies is acceptable and there is no need for repeated fumigation. If more fumigation is required. For fungal interpretation More than 2 colonies appear which is not acceptable and fumigation is required again.
- 9. After 24-36hrs check the results If growth of any colony appears with limited amount, It would show positive results ( means now lab is ready for aseptic conditions experiments) If growth of microbial colony appears more than limited amount, it would show negative results ( means contaminants still present)
- 10. For eliminating the contaminants, repeat the fumigation
- 11. PRECAUTIONS • PPE kit should be wear before entering the fumigated area. • Glass-wares used for post fumigation should be sterilised. • Pouring the media into the petri-plates should be done in laminar hood. • UV light should be switched off before using the laminar hood. • Maintain the other chemicals which should not placed in fumigation otherwise chances of explosion may occur.
- 12. THANKYOU