PCR (polymerase chain reaction) is a technique used to amplify a specific DNA sequence. It was invented in 1983 by Kary Mullis, who received the Nobel Prize for it. PCR uses DNA polymerase to exponentially replicate a target DNA sequence by cycling between high and low temperatures. It requires a DNA template, primers that define the target sequence, DNA building blocks (dNTPs), a thermostable DNA polymerase, magnesium ions, and a buffer solution. During cycling, the DNA is denatured, the primers anneal, and the polymerase extends the DNA. This process doubles the DNA after each cycle, allowing millions of copies to be produced rapidly for analysis using gel electrophoresis, sequencing, or other methods. PCR