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PHYSICAL
MAPPING
Dr. Priya Trivedi
M.Sc.(BOTANY), M.Ed., Ph.D. (BOTANY)
A physical map gives the actual distances in number
of bases between genes on a chromosome.
As such, it describes the structural characteristics of
the chromosome itself.
The physical map is one in which chromosomes are
cut into large fragments, then copied (or cloned) many
millions of times. The copies are then analyzed with a
goal of finding segments that overlap.
Rearranging these overlapping segments reconstitutes
the chromosome.
Distance is expressed in Megabase pairs ( need not be
proportionate to recombination frequencies).
Restriction
Mapping
Fluorescence in situ
Hybridization (FISH)
Sequence tag sites or STSs
Chromosome
walking
Principle
Identification of fragments with overlapping
sequences may be a key to the reconstruction
or characterization of large chromosome
regions.
Using the conventional cloning techniques to
isolate fragments, one may be able to isolate
>100kb chromosome region per month.
In addition, pulsed field gel electrophoesis
may be used to isolated >100 kb DNA
fragments, which may be cloned.
THANK U

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Physical mapping

  • 2.
  • 3.
  • 4.
  • 5. A physical map gives the actual distances in number of bases between genes on a chromosome. As such, it describes the structural characteristics of the chromosome itself. The physical map is one in which chromosomes are cut into large fragments, then copied (or cloned) many millions of times. The copies are then analyzed with a goal of finding segments that overlap. Rearranging these overlapping segments reconstitutes the chromosome. Distance is expressed in Megabase pairs ( need not be proportionate to recombination frequencies).
  • 6.
  • 7.
  • 8.
  • 9.
  • 11.
  • 12.
  • 13.
  • 14.
  • 16.
  • 17.
  • 18.
  • 19.
  • 20.
  • 21.
  • 23.
  • 24.
  • 25.
  • 26.
  • 27.
  • 29. Principle Identification of fragments with overlapping sequences may be a key to the reconstruction or characterization of large chromosome regions. Using the conventional cloning techniques to isolate fragments, one may be able to isolate >100kb chromosome region per month. In addition, pulsed field gel electrophoesis may be used to isolated >100 kb DNA fragments, which may be cloned.
  • 30.
  • 31.
  • 32.
  • 33.
  • 34.
  • 35.
  • 36.