Discovery of nucleus
Evolution of nucleus
Structure of nucleus
Function of nucleus
Diseases related with nucleus
The nucleus was the first organelle to be discovered.
The probably oldest preserved drawing dates back to the early microscopist Antonie van Leeuwenhoek (1632 – 1723). He observed a "Lumen", the nucleus, in the red blood cells of salmon.
The nucleus was also described in 1831 by Scottish botanist Robert Brown.
Brown was studying orchids under microscope when he observed an opaque area, which he called the areola or nucleus, in the cells of the flower's outer layer. He did not suggest a potential function.
The cell nucleus is a membrane bound structure that:-
Contains the cell's hereditary information
Controls the cell's growth and reproduction
Direct the other components of cell through protein regulation
Make Proteins through central dogma of cell
DNA mRNA Protein
Emery-Dreifuss muscular dystrophy
Mutations in nuclear lamins associated with Emery-Dreifuss muscular dystrophy.
Emery-Dreifuss muscular dystrophy (EDMD) is a neuromuscular degenerative condition with an associated dilated cardiomyopathy and cardiac conduction defect.
It can be inherited in either an X-linked or autosomal manner by mutations in the nuclear proteins emerin and lamin A/C, respectively.
Traditionally muscular dystrophies were associated with defects in sarcolemma-associated proteins and, therefore, a nuclear connection suggested the existence of novel signalling pathways associated with this group of diseases.
Subsequently, other mutations in the lamin A/C gene were attributed to a range of tissue-specific degenerative conditions, collectively known as the 'laminopathies’.
Presentation include Nucleus and its components like nuclear envelope, nucleolus, chromatin fibers, ultra structure of nucleus and its general functions.
Presentation include Nucleus and its components like nuclear envelope, nucleolus, chromatin fibers, ultra structure of nucleus and its general functions.
Conformational study of polynucleotideKAUSHAL SAHU
Introduction
History
The conformation of nucleic acid
Types of polynucleotide
DNA- types and conformation of DNA
B- DNA
A-DNA Z-DNA
RNA – types and conformation of RNA
Coding DNA
Non coding DNA
structure of RNA
Primary structure of RNA
Secondary structure of RNA
Tertiary structure of RNA
Analyzing techniques
Conclusion
References
Nucleus: Structure and function
nuclear membrane
nuclear lamins
Nuclear pore complexe
nuclear matrix, composition and its role
cajal bodies
SFCs
nuclear speckles
PML bodies
Nucleolus
Conformational study of polynucleotideKAUSHAL SAHU
Introduction
History
The conformation of nucleic acid
Types of polynucleotide
DNA- types and conformation of DNA
B- DNA
A-DNA Z-DNA
RNA – types and conformation of RNA
Coding DNA
Non coding DNA
structure of RNA
Primary structure of RNA
Secondary structure of RNA
Tertiary structure of RNA
Analyzing techniques
Conclusion
References
Nucleus: Structure and function
nuclear membrane
nuclear lamins
Nuclear pore complexe
nuclear matrix, composition and its role
cajal bodies
SFCs
nuclear speckles
PML bodies
Nucleolus
STRUCTURE AND FUNCTIONS OF NUCLEUS OF A CELL.pptxRASHMI M G
The nucleus is the part of a cell that contains DNA organized into chromosomes and is located in the middle of the cell. It is surrounded by the nuclear envelope, which is a double membrane that separates the nucleus from the cytoplasm. The nuclear envelope contains nuclear pores, which are gateways that allow molecules to move into and out of the nucleus.
The Cell: The Histology Guide
Nucleus - The Cell: The Histology Guide - University of Leeds
The nucleus is found in the middle of the cells, and it contains DNA arranged in chromosomes. It is surrounded by the nuclear envelope, a double nuclear membrane (outer and inner), which separates the nucleus from the cytoplasm. The outer membrane is continuous with the rough endoplasmic reticulum.
open.baypath.edu
Nucleus – BIO109 Biology I Introduction to Biology
The boundary of the nucleus, called the nuclear envelope, is a double membrane that contains small openings called nuclear pores. These pores are gateways that allow molecules to move into and out of the nucleus, enabling it to communicate with the rest of the cell.
The nucleus has three main parts:
Nuclear membrane: A protective barrier of the nucleus
Nucleoplasm: The cytoplasm of the nucleus, which is a semifluid matrix that contains chromatin, the less condensed form of DNA that organizes into chromosomes during mitosis or cell division
Nucleolus: A spherical structure that produces and assembles the cell's ribosomes
The nucleus controls and regulates the activities of the cell, such as growth and metabolism.
What are the 4 types of nucleus?
What are the 3 parts of a nucleus?
How many nuclei are in a cell?
Ask a follow up
Nucleus-the heart of the cell-cellular organellesbiOlOgyBINGE
In cell biology, the nucleus is a membrane-bound organelle found in eukaryotic cells.
The nucleus is found in all the eukaryotic cells of the plants and animals.
here u will find every detail of nucleus.
for more details ,visit @biOlOgy BINGE-insight learning (youtube channel)
DNA
its Discovery
Who Discovered DNA?
Credit for who first identified DNA is often mistakenly given to James Watson and Francis Crick, who just furthered Miescher’s discovery with their own groundbreaking research nearly 100 years later. Watson and Crick contributed largely to our understanding of DNA in terms of genetic inheritance, but much like Miescher, long before their work, others also made great advancements in and contributions to the field.
In 1866, before many significant discoveries and findings, Gregor Mendel was the first to suggest that characteristics are passed down from generation to generation. Mendel coined the terms as recessive and dominant.
In 1869, Friedrich Miescher identified the “nuclein” by isolating a molecule from a cell nucleus that would later become known as DNA.
In 1881, Nobel Prize winner and German biochemist Albrecht Kossel, who is credited with naming DNA, identified nuclein as a nucleic acid. He also isolated those five nitrogen bases that are now considered to be the basic building blocks of DNA and RNA: adenine (A), cytosine (C), guanine (G), thymine (T) and uracil (U) in case of RNA).
In 1882, Walther Fleming devoted research and time to cytology, which is the study of chromosomes. He discovered mitosis in 1882 when he was the first biologist to execute a wholly systematic study of the division of chromosomes. His observations that chromosomes double is significant to the later discovered theory of inheritance.
In Early 1900s, Theodor Boveri and Walter Sutton were independently working on what’s now known as the Boveri-Sutton chromosome theory, or the chromosomal theory of inheritance. Their findings are fundamental in our understanding of how chromosomes carry genetic material and pass it down from one generation to the next.
In 1902, Mendel’s theories were finally associated with a human disease by Sir Archibald Edward Garrod, who published the first findings from a study on recessive inheritance in human beings in 1902. Garrod opened the door for our understanding of genetic disorders resulting from errors in chemical pathways in the body.
In 1944, Oswald Avery first outlined DNA as the transforming principle, which essentially means that DNA transform cell properties.
Nucleus” is a Latin word meaning Kernel
It is the “CONTROL CENTER” of the cell
Average diameter of nucleus is 6um, which occupies around 10% of cell volume
Nuclear Envelope
Nuclear Pores and complex
Nuclear lamina
Chromosomes & Chromatin
Nucleolus
Nucleoplasm
it gives information about the nucleus which is the major cell component and its various parts like nuclear pores,nucleolus etc. it helps in the replication of DNA which contain genetic material.
Optimality theory
Optimal expression level of a protein under constant conditions
Cost of the LacZ Protein
Mathematical description of cost function
The Benefit of the LacZ Protein
Fitness Function and the Optimal Expression Level
Cells Reach Optimal LacZ Levels in a Few Hundred Generations in Laboratory Evolution Experiments
Environmental selection of the feedforward loop network motif
References
Assisted Reproductive Technologies (ART)
First Successful IVF: Birth of Louise Brown in 1978
Rapid developments in the field of ART
Moral panic
Ethics
Ethical issues
Ethical concerns
Moral issues
Social issues
Religion
Case study
The manipulation and analysis of chemical structural information is made possible through the use of molecular descriptors.
These are numerical values that characterise properties of molecules.
For example, they may represent the physicochemical properties of a molecule or they may be values that are derived by applying algorithmic techniques to the molecular structures.
Many different molecular descriptors have been described and used for a wide variety of purposes.
They vary in the complexity of the information they encode and in the time required to calculate them.
In general, the computational requirements increase with the level of discrimination that is achieved. For For example, the molecular weight does not convey much about a molecule’s properties but it is very rapid to compute.
By contrast, descriptors that are based on quantum mechanics may provide accurate representations of properties, but they are much more time consuming to compute.
Some descriptors have an experimental counterpart (e.g.the octanol–water partition coefficient), whereas others are purely algorithmic constructs (e.g. 2D fingerprints).
Apriori is the most famous frequent pattern mining method. It scans dataset repeatedly and generate item sets by bottom-top approach.
Apriori algorithm is given by R. Agrawal and R. Srikant in 1994 for finding frequent itemsets in a dataset for boolean association rule. Name of the algorithm is Apriori because it uses prior knowledge of frequent itemset properties
Epidemiology, Genetic Recombination, and Pathogenesis of CoronavirusesGaurav Aggarwal
Since the starting of the Coronavirus Pandemic around the world, Everyone wants to know about coronavirus nCoV-19. This ppt will cover different strains of Coronaviruses that have been discovered since 1966 - Their Epidemiology and Pathophysiology.
Discussion about the pandemics and epidemics that happened before the novel coronavirus pandemic. While the main focus of the class will be on :
(1) The causative strains of coronaviruses that were involved to cause those pandemics/ epidemics.
(2) Genetics recombination among them to evolve more pathogenic strains.
The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
In this talk, I will discuss our work in (1) building an integrative knowledge infrastructure to prepare FAIR and "AI-ready" data and services along with (2) neurosymbolic AI methods to improve the quality of predictions and to generate plausible explanations. Attention is given to standards, platforms, and methods to wrangle knowledge into simple, but effective semantic and latent representations, and to make these available into standards-compliant and discoverable interfaces that can be used in model building, validation, and explanation. Our work, and those of others in the field, creates a baseline for building trustworthy and easy to deploy AI models in biomedicine.
Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
2. Contents
Discovery of nucleus
Evolution of nucleus
Structure of nucleus
Function of nucleus
Diseases related with nucleus
3. Discovery of
Nucleus
The nucleus was the first organelle to be discovered.
The probably oldest preserved drawing dates back to the
early microscopist Antonie van Leeuwenhoek (1632 –
1723). He observed a "Lumen", the nucleus, in the red blood
cells of salmon.
The nucleus was also described in 1831 by Scottish
botanist Robert Brown.
Brown was studying orchids under microscope when he
observed an opaque area, which he called the areola or
nucleus, in the cells of the flower's outer layer. He did not
suggest a potential function.
4. What is
nucleus of a
cell?
The cell nucleus is a membrane bound structure that:-
Contains the cell's hereditary information
Controls the cell's growth and reproduction
Direct the other components of cell through
protein regulation
Make Proteins through central dogma of cell
DNA mRNA Protein
5. Evolution of
Nucleus
Four major hypotheses have been proposed to explain the existence of
the nucleus :-
Syntrophic model : proposes that a symbiotic relationship
between the archaea and bacteria created the nucleus-containing
eukaryotic cell.
Second model : proposes that proto-eukaryotic cells evolved from
bacteria without an endosymbiotic stage.
Viral eukaryogenesis model : proposes that the membrane-bound
nucleus, along with other eukaryotic features, originated from the
infection of a prokaryote by a virus. The suggestion is based on
similarities between eukaryotes and viruses such as linear DNA
strands, mRNA capping, and tight binding to proteins
(analogizing histones to viral envelopes).
Exomembrane hypothesis : suggests that the nucleus instead
originated from a single ancestral cell that evolved a second exterior
cell membrane; the interior membrane enclosing the original cell
then became the nuclear membrane and evolved increasingly
elaborate pore structures for passage of internally synthesized
cellular components such as ribosomal subunits.
6. Structure of
nucleus
The nucleus is the largest organelle in animal cells.
In mammalian cells, the average diameter of the nucleus is
approximately 6 micrometres (µm), which occupies about
10% of the total cell volume.
The contents of the nucleus are held in
the nucleoplasm similar to the cytoplasm in the rest of the
cell. The fluid component of this is termed as the nucleosol,
similar to the cytosol in the cytoplasm.
8. Contd…
An electron micrograph of a section through an animal cell nucleus (from an insect
cell) showing heterochromatin (H), euchromatin (E) and the nucleolus with its fibrous
centre (FC) and peripheral fibrous region (PF).
9. Components
of nucleus
1.Nuclear envelope
Structural feature:
The nuclear envelope, otherwise known as nuclear membrane,
consists of two cellular membranes, an inner and an outer
membrane, arranged parallel to one another.
The space between the membranes is called the perinuclear space
and is continuous with the RER lumen
Functional aspect:
The nuclear envelope completely encloses the nucleus and
separates the cell's genetic material from the surrounding
cytoplasm, serving as a barrier to prevent macromolecules from
diffusing freely between the nucleoplasm and the cytoplasm.
10. Contd…
2. Nuclear pores
Structural feature:
Nuclear pores provide aqueous channels through the envelope.
The pores are 100 nm in total diameter.
The gap through which molecules freely diffuse is only about 9 nm
wide.
A number of 3000 to 4000 pores are present in the envelope,
each of which contains an eightfold-symmetric ring-shaped
structure at a position where the inner and outer membranes fuse.
Functional aspect:
passage of small water-soluble molecules while preventing larger
molecules, such as nucleic acids and larger proteins, from
inappropriately entering or exiting the nucleus.
Most proteins, ribosomal subunits, and some DNAs are
transported through the pore complexes.
11. Contd…
3.Nuclear lamina
Structural feature :
The nuclear lamina forms an organized meshwork on the internal
face of the envelope, while less organized support is provided on
the cytosolic face of the envelope. nuclear lamina is composed
mostly of lamin proteins.
Like all proteins, lamins are synthesized in the cytoplasm and later
transported to the nucleus interior, where they are assembled
before being incorporated into the existing network of nuclear
lamina
Functional aspect : provide structural support for the nuclear
envelope and anchoring sites for chromosomes and nuclear pores
12. Contd…
4.Nucleolus
Structural Feature:
The nucleolus is the largest of the discrete densely stained,
membraneless structures known as nuclear bodies found in the
nucleus.
It forms around tandem repeats of rDNA, DNA coding for ribosomal
RNA (rRNA). These regions are called nucleolar organizer
regions(NOR)
When observed under the electron microscope, the nucleolus can be
seen to consist of three distinguishable regions: the innermost fibrillar
centers (FCs), surrounded by the dense fibrillar component (DFC) (that
contains fibrillarin and nucleolin), which in turn is bordered by
the granular component (GC) (that contains the
protein nucleophosmin).
Functional aspect:
Main roles of the nucleolus are to synthesize rRNA and assemble
ribosomes.
13. Contd…
5.Chromosomes
Structural feature:
The cell nucleus contains the majority of the cell's genetic material in the form
of multiple linear DNA molecules organized into structures
called chromosomes.
Each human cell contains roughly two meters of DNA.
During most of the cell cycle these are organized in a DNA-protein complex
known as chromatin, and during cell division the chromatin can be seen to
form the well defined chromosomes familiar from a karyotype.
A small fraction of the cell's genes are located instead in the mitochondria.
There are two types of chromatin, Euchromatin is the less compact DNA
form, and contains genes that are frequently expressed by the cell.The other
type, heterochromatin, is the more compact form, and contains DNA that is
infrequently transcribed.
During interphase the chromatin organizes itself into discrete individual
patches,[called chromosome territories.Active genes, which are generally
found in the euchromatic region of the chromosome, tend to be located
towards the chromosome's territory boundary.
14. Chromosomes
c0ntd…
Functional aspect:
Chromosome is the most condensed form of DNA . So its
function lies in the fact that which are performed by DNA .
The chromosome holds not only the genetic code, but many
of the proteins responsible for helping express it. Its complex
form and structure dictate how often genes can be translated
into proteins, and which genes are going to be translate.
15. Diseases
related to
Nucleus
Emery-Dreifuss muscular dystrophy
Mutations in nuclear lamins associated with Emery-Dreifuss
muscular dystrophy.
Emery-Dreifuss muscular dystrophy (EDMD) is a neuromuscular
degenerative condition with an associated dilated
cardiomyopathy and cardiac conduction defect.
It can be inherited in either an X-linked or autosomal manner by
mutations in the nuclear proteins emerin and laminA/C,
respectively.
Traditionally muscular dystrophies were associated with defects
in sarcolemma-associated proteins and, therefore, a nuclear
connection suggested the existence of novel signalling pathways
associated with this group of diseases.
Subsequently, other mutations in the laminA/C gene were
attributed to a range of tissue-specific degenerative conditions,
collectively known as the 'laminopathies’.
16. Down
syndrome
Down syndrome is a genetic disorder caused when abnormal cell
division results in an extra full or partial copy of chromosome 21.
This extra genetic material causes the developmental changes
and physical features of Down syndrome.
Down syndrome varies in severity among individuals, causing
lifelong intellectual disability and developmental delays.
It's the most common genetic chromosomal disorder and cause of
learning disabilities in children.
It also commonly causes other medical abnormalities, including
heart and gastrointestinal disorders.
17. Phenylketonuria
Disease (PKU)
Phenylketonuria (PKU) is an inborn error of metabolism that
results in decreased metabolism of the amino acid phenylalanine.
Untreated, PKU can lead to intellectual disability, seizures,
behavioral problems, and mental disorders.
It may also result in a musty smell and lighter skin.
A baby born to a mother who has poorly treated PKU may have
heart problems, a small head, and low birth weight.
Phenylketonuria is a genetic disorder inherited from a person's
parents.
It is due to mutations in the PAH gene, which results in low levels
of the enzyme phenylalanine hydroxylase.
This results in the buildup of dietary phenylalanine to potentially
toxic levels.
It is autosomal recessive, meaning that both copies of the gene
must be mutated for the condition to develop