Surface area is an important physical property that influences the reactivity, dissolution, catalysis, and separation of materials. The surface area often must be carefully engineered and measured to optimize specific functions. In this Webinar, our applications lab will explain with real-world examples:
- Physical adsorption technique - BET theory
- Sample preparation – the start of a good measurement
- Calculating specific surface area from gas adsorption on solid surfaces
- Troubleshooting – what happens when things go wrong?
View recorded webinars:
http://bit.ly/particlewebinars
This presentation includes basis of lithography i.e. (photo-lithography e-beam lithography) in nano-lithography includes (AFM, Soft, NIL and DPN lithography)
Discussion about hydrothermal & gel growth method of crystalMostakimRahman1
1.Definition, procedure, advantage, and disadvantage of hydrothermal growth method of crystal.
2.Definition, procedure, advantage, and disadvantage of gel growth method of crystal.
Hot wall reactor is a high temperature chamber in which the substrate is placed for coating. In this reactor including the substrate, all other parts (inlet and outlet tubes) inside the chamber get coated.
INCLUDES THE INTRODUCTION TO CRYSTALLIZATION, FOLLOWED BY MECHANISM LIKE SUPER SATURATION, NUCLEUS FORMATION, CRYSTAL GROWTH, IN DETAIL ACCOUNT HOMOGENOUS AND HETEROGENOUS NUCLEATION AS PRIMARY AND SECONDARY NUCLEATION.
Surface area is an important physical property that influences the reactivity, dissolution, catalysis, and separation of materials. The surface area often must be carefully engineered and measured to optimize specific functions. In this Webinar, our applications lab will explain with real-world examples:
- Physical adsorption technique - BET theory
- Sample preparation – the start of a good measurement
- Calculating specific surface area from gas adsorption on solid surfaces
- Troubleshooting – what happens when things go wrong?
View recorded webinars:
http://bit.ly/particlewebinars
This presentation includes basis of lithography i.e. (photo-lithography e-beam lithography) in nano-lithography includes (AFM, Soft, NIL and DPN lithography)
Discussion about hydrothermal & gel growth method of crystalMostakimRahman1
1.Definition, procedure, advantage, and disadvantage of hydrothermal growth method of crystal.
2.Definition, procedure, advantage, and disadvantage of gel growth method of crystal.
Hot wall reactor is a high temperature chamber in which the substrate is placed for coating. In this reactor including the substrate, all other parts (inlet and outlet tubes) inside the chamber get coated.
INCLUDES THE INTRODUCTION TO CRYSTALLIZATION, FOLLOWED BY MECHANISM LIKE SUPER SATURATION, NUCLEUS FORMATION, CRYSTAL GROWTH, IN DETAIL ACCOUNT HOMOGENOUS AND HETEROGENOUS NUCLEATION AS PRIMARY AND SECONDARY NUCLEATION.
Crystallization is a separation process very commonly used in the industry of many different materials, from commercially very common chemicals to very specific ones. It also plays an important role in the pharmaceutical industry, as more than 90% of active pharmaceutical ingredients (API) are synthesized as a crystalline product. Crystallization may have a significant direct and indirect influence on the quality of a product; therefore, it is one of the most important purification and separation methods in the production of APIs.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
In this talk, I will discuss our work in (1) building an integrative knowledge infrastructure to prepare FAIR and "AI-ready" data and services along with (2) neurosymbolic AI methods to improve the quality of predictions and to generate plausible explanations. Attention is given to standards, platforms, and methods to wrangle knowledge into simple, but effective semantic and latent representations, and to make these available into standards-compliant and discoverable interfaces that can be used in model building, validation, and explanation. Our work, and those of others in the field, creates a baseline for building trustworthy and easy to deploy AI models in biomedicine.
Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
1. NUCLEATION
1
Presented to : Dr. Renu Chadha
Professor ,UIPS, Panjab University
SEMINAR ON
Email: shubham00sharma70@gmail.com
Presented by: Shubham Sharma
Pharmaceutical Chemistry
Roll no. : 20029
3. 3
•
Crystallization is a chemical solid–liquid separation technique, in which mass
transfer of a solute from the liquid solution to a pure solid.
Crystallization is a separation technique that is used to separate solid that has dissolved
in a liquid. The solution is warmed in an open container, allowing the solvent to
evaporate, leaving asaturatedsolution.
As the saturated solution is allowed to cool, the solution will separate out of the
solution andcrystalswill start to grow. Thecrystalscanbecollected and allowed to dry.
CRYSTALLIZATION
4. 4
Crystalcanbe defined asa
solid particle, which is
formed by the
solidification process
under suitable
environment in which
structural units are
arranged by a fixed
geometric pattern or
lattice.
CRYSTAL STRUCTURE OFNaCl
CRYSTALS :
6. 6
Step where solute molecules dispersed in the solvent start to gather into clusters on the nanometer scale.
Someclusters may become sobig that they may arrange themselves in lattice arrangement. Thesebodies of
aggregatesarecalled embryo. However,embryos areunstableandthey maybreak into clustersagain.
Thesestablestructurestogether form a nuclei.
Nucleationisgreatlyinfluencedbytemperature, supersaturation and impurities.
It is at the stage of nucleation that atoms arrange in periodic manner to form crystal structure. If all sources of
partition are subsumed under the term nucleation, a number of kinds of nucleation may occur.
They may be classified into two groups :
1. Primary nucleation
2. Secondary nucleation
NUCLEATION
7. PRIMARY NUCLEATION
The phenomenon of the nucleation is the same for crystallization from the
solution , crystallization from a melt , condensation of fog drops in a super cooled
vapor and generation of bubble In a supersaturated liquid.
There are two types :
a) Homogeneous nucleation
b) Heterogeneous nucleation
8. HOMOGENEOUS NUCLEATION
In crystallization from solution , homogenous nucleation almost never happens, except
perhaps in some precipitation reaction.
Initially several molecule or ions or atoms associated to from clusters.
These are loose aggregates, which usually disappear quickly.
When enough particles associate to form a embryo , then is beginning of the lattice
arrangement and the formation of new solid phase .
The initially formed crystals are of molecular size, which are termed as nuclei.
9. If effect holds that if the nucleus wets the surface of the catalyst , then
nucleation formation is reduced by a factor that is a function of the angle of
wetting between the nucleus and the catalyst .
Experiment data on the heterogeneous nucleation of potassium chloride solution
shows that the nucleation of the substance is consists with an apparent value of
the interfacial tension in the range 2 to 3 ergs/Cm2 for both catalyzed nucleation
and nucleation without an added catalyst.
HETREROGENOUS NUCLEATION
10. SECONDARY NUCLEATION
The formation of nuclei attributable to the influence of the existing macroscopic
crystal the magma is called secondary nucleation.
Fluid share nucleation: when supersaturated solution moves past the surface of a
growing crystals at substantial velocity, the shear stresses in the boundary layer
may sweep away embryos or nuclei that would otherwise be incorporated into the
growing crystals and so appear as new crystals.
This has been reported in work on sucrose crystallization.
12. PRESENCE OF ANOTHER SUBSTANCE IN MOTHER
LIQUOR:
12
Sodium chloride crystallized from aqueous solutions
produces cubic crystals.
If sodium chloride is crystallized from a solution containing
a small amount of urea, the crystals obtained will have
octahedral faces. Both types of crystals belong to the cubic
crystal form but differ in habit.
13. 13
Moderate solubility is best (avoidsuper saturation).Likedissolves like.
Hydrogen bonding canhelp or hinder crystallization.
Presenceof benzenecanhelp crystal growth. Avoid highly volatile solvents.
Avoid long chain alkyl solvents canbesignificantly disordered in crystals.
Choosesolvents with “rigid geometries”.
SOLVENT CONSIDERATIONS
15. CRYSTALGROWTH
15
Crystals grow by the ordered deposition of the solute molecules onto the
surface of apre-existing crystal.
Crystal growth is facilitated by the environment changing slowly over time.
Keep crystal growth vessel away from sources of mechanical agitation (e.g.
vibrations).
Set-up away from vacuum pumps, rotovaps, hoods, doors, drawers, and so on
Leave samplesalone for 1 week, don't “check in” with it.
16. TIME
Thelonger the time, the better the crystals.
Faster crystallization is not as good as slow crystallization. Faster crystallization higher
chanceof lower quality crystals
16
Quality crystals grow best over time in near equilibrium conditions.
17. 17
Purification of drugs.
Improve bioavailability of the drug andchoosethe most stable form.
Acrystalline powder is easily handled ,stable , possessesgood flow properties and an attractive appearance
.
Crystallization from solution is important industrially because of the variety of materials that are
marketed in the crystallineform.
Crystallization affords a practical method of obtaining pure chemical substances in a satisfactory
condition for packaging and storing. A crystal formed from an impure solution is itself pure (unless
mixed crystals occur).
IMPORTANCE OFCRYSTALLIZATION
18. 18
Adrug mayremain in different crystalline forms, someare stable,and rests are metastable.
The metastable forms have greater solubility in water, thus have better bioavailability. By
controlling the conditions during crystallization, the quantity of metastable to stable forms
maybecontrolled.
After crystallization water or solvent molecules may be entrapped within the crystal
structure and thus form hydrates or solvates which have different physical properties that
maybe utilized in various pharmaceutical purpose.
Particles with various micromeritic properties, compressibility and wettability can be
prepared by controlling the crystallization process.
19. References
Martin D. Johnson, Christopher L. Burcham, Scott A. May, Joel R. Calvin, Jennifer McClary Groh, Steven S.
Myers, Luke P. Webster, Jeffrey C. Roberts, Venkata Ramana Reddy, Carla V. Luciani, Aoife P. Corrigan, Richard
D. Spencer, Robert Moylan, Raymond Boyse, John D. Murphy, James R. Stout. API Continuous Cooling and
Antisolvent Crystallization for Kinetic Impurity Rejection in cGMP Manufacturing. Organic Process Research &
Development 2021, Article ASAP.
Cedric Devos, Tom Van Gerven, Simon Kuhn. A Review of Experimental Methods for Nucleation Rate
Determination in Large-Volume Batch and Microfluidic Crystallization. Crystal Growth & Design 2021, 21 (4) ,
2541-2565. https://doi.org/10.1021/acs.cgd.0c01606
Jaka Orehek, Dušan Teslić, Blaž Likozar. Continuous Crystallization Processes in Pharmaceutical Manufacturing: A
Review. Organic Process Research & Development 2021, 25 (1) , 16-42. https://doi.org/10.1021/acs.oprd.0c00398
https://www.slideshare.net/JaveriaMalik16/crystallization-crystals
https://www.slideshare.net/ShikhaPopali1/crystallization-easily-described