Molecular breeding in legumes for resource-poor farmers: Chickpea for Ethiopia and India presentation by "Douglas Cook, University of California Davis, Davis,
United States of America"
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Molecular breeding in legumes for resource-poor farmers: Chickpea for Ethiopia and India
1. http://chickpealab.ucdavis.edu
United States, Ethiopia, India, Pakistan, Turkey, Australia, Canada,
Morocco
Domestication Modern breeding
Regionaldiversification
WildRelatives
Abiotic stress, Biotic stress, Nitrogen fixation, Nutrition, Agronomic traits
Molecular breeding in legumes for resource-poor
farmers: Chickpea for Ethiopia and India
2. Chickpea is the world’s 2nd most important
grain legume and critical to food security in
much of the developing world
•Stagnant yields
•Susceptible to pathogens,
pests and abiotic stress
• Drought
• Heat
• Pests and Disease
• Nitrogen fixation
• Nutrition
• Soil adaptation
• Domestication
11
5. Our target countries
• Regularly impacted by climatic
extremes
Ethiopia
• 40% of Africa’s chickpea
production
• Historical focus of
domestication
India
• Major consumer and producer
of chickpea.
• Among the lowest yielding
countries.
7. Plant a er the rains.
Produce crop on residual soil moisture.
Exposes crop to drought.
8. Ascochyta blight is a climate-driven disease, and o en
the limi ng factor in chickpea produc on
Fusarium wilt
Pod borer
9. Plant a er the rains
Climate-driven shi s in the microbiome drive cul va on prac ces
Early plan ng increases risk of:
1. Foliar Ascochyta blight
2. Soil seedling diseases
Both diseases are driven by
high moisture.
Cul va on too late in season =
increased risk of:
1. Drought and Heat
2. Fusarium infec on
3. Pod borer
Abiotic and biotic stress drive cultivation practices
11. Wild systems: Starting in south-eastern Turkey
Turkey
Syria Iraq
Iran
C.arietinum
C.reticulatum
C.echinospermum
Using all 19 variables for Bioclim on DIVA-GIS
13. Time-series analyses: how effects of G changed
over ~100 years of climate / environment change
0
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3000 1911
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#ofAccessions
# Date of acquisition
Percentofcollection
Ethiopia*(87)
India
(261)
Turkey (75)
39
14. We explored chickpea’s center of origin over 56 days
in 2013 and 100 days in 2014/15 at ~50 sites
Egil
1 2
3
4
5
6
C.arietinum
C.reticulatum
C.echinospermum
In the wild: 1002 Km
15. How do you survey variation for climate
resilience?
Altitude: 600m – 2,000m
Rainfall gradients
Seasonality
Temperature
Humidity
Soil types
Microbial communities
Co-occurring species
17. DNA from thousands
GBS and WGS
population genomics
in situ association genetics
C. reticulatum
C. echinospermum
Egil
1 2
3
4
5
6
Predominant focus of
historical germplasm
1,100 accessions 2013
~1,000 accessions 2014/2015
Cicer reticulatumCicer echinospermum
Karabace
S2DR
Gunas
Cermic
Destek
Guven
Derici
Kesantas
Oyali
Sarikaya
Kayatape
Kalkan
Egil
Besevler
Savur
Bari2
Bari3
Bari1
Cudi1
Cudi2
Sirnak
Ortanca
RKesen
RCNTRL
REgill
RKalka
RBesev
RSavur
RKayat
Rnpgs
RDerei
RSarik
RBari3
RBari2
RBari1
ROyali
RCudiA
RCudiB
RSirna
EDeste
EGunas
ECermi
Enpgs
ERdsde
ES2Drd
EKarab
EOrtan
EGuven
18. How will we make the wild alleles
useful for breeding?
19. Evaluation Strategies
In situ association genetics for
candidate gene nomination
Association genetics with
phenology-normalized NAMs
Advanced backcross
introgression lines
Breeding
Ecology and population
genomics
Phenotyping
Trait discovery
Genomics-driven
Introgression
20. Domestication:
10-12,000 years ago
Secondary diversification:
~6,000 years ago
Secondary diversification:
~3-4,000 years ago
6.7M variants
0.172 M variants
•3 High quality reference genomes:
BioNano OM, PacBio, Illumina,
dense SNP maps
•26 deep representative wilds (30X)
•250 moderate wilds (10X)
•750 shallow wilds with imputation
•29 cultivated from international
project
Developing genomic platforms
Wild species Modern elite varieties
~95% loss of variation
25. Abiotic stress: Drought, heat, cold
Biotic stress: Fusarium, Pod borer, (Ascochyta)
Nitrogen: Symbiosis and nitrogen “metabolism”.
Nutrition: Inorganic and organic composition.
Agronomic traits: Architecture, flowering time.
Phenotyping and trait targets
26. UC Davis
Doug Cook
Varma Penmetsa
Noelia Carrasquilla
Alex Greenspan
Betsy Alford
Susan Moenga
Lisa Vance
Peter Chang
Bullo Mamo
Brendan Riely
Gul Abbas
Dagnachew Bekele
Zahra Samiezade-Yazd
Lei Feng
Ping Song
Shraddha Suman
Florida International Univ
Eric von Wettberg
ICRISAT
Vincent Vadez
Hari Sharma
Assam Agric Univ
Bidyut Sarmah
Punjab Agric Univ
Sarvjeet Singh
Harran University
Adbullah Kahraman
Dicle University
Bekir Bukun
Fatma Basdemin
AARI
Ali Peksuslu
Lerzan Aykas
Abdullah Inan
Turkish Ministry of Ag
Abdulkadir Aydogan
Husseyin Ozcelik
Mahmut Gayberi
CSIRO WA
Jens Berger
John Thompson
Wendy Vance
Judith Lichtenzveig
Graham O’Hara
Banaras Hindu University
Birinchi Sarma
UAS-Dharwad
Bhuvaneshwara Patil
EIAR
Asnake Fikre
Lijalem Balcha
Kassaye Dinegde
Zehara Damtew
Dagnachew Bekele
Tsegaye Getahun
Gashaw Bedada
Sultan Yimer
Addis Aababa Univ
Kassahun Tesfaye
Fassil Assefa
Masresha Fetene
USC
Sergey Nuzhdin
Peter Chang
Vasantika Singh
Matilde Cordeiro
Min-Gyoung Shin
ICARDA
Michel Ghanem
Sripada Udupa
Ege University
Bhattin Tanyolac
U Saskatchewan
Bunyamin Taran
Research Partners and Institutions