This document summarizes research on phenotyping common bean populations and breeding lines for drought and bean stem maggot (BSM) tolerance in Ethiopia. Two populations consisting of 82 and 100 recombinant inbred lines were evaluated at three locations under different stress conditions. Data was collected on various traits related to drought and BSM tolerance. The populations showed variability in their performance. Additionally, 36 Andean genotypes were evaluated for drought tolerance traits under irrigated and stressed conditions. A marker-assisted recurrent selection scheme was used to develop populations for further evaluating drought tolerance. The research was supported by various collaborators and organizations.

1. Phenotyping common bean populations and breeding
lines for drought and BSM tolerance in Ethiopia
Asrat AA, Fistum A, Daniel A, Yayis R
SARI- Ethiopia

2. Act. 3: Deploy insect and disease resistance genes
Milestone 5: QTL for bean stem
maggot resistance
• Two populations evaluated for BSM
and drought combined stress in Ethiopia
at three locations as part of M.Sc.
Objectives:
Thesis
• To estimate the phenotypic and genotypic
• 14 released varieties evaluated for variability for Morpho-physiological
BSM and drought combined stress traits and asses the genetic variability for
tolerance in Ethiopia at three locations drought and BSM resistance among
common bean recombinant lines
• To evaluate the performance of the
recombinant lines of two populations
from crosses G2333 x G19839 and
BAT881 x G21212 for their drought and
BSM combined resistance.
• To evaluate the contribution of
adventitious roots for BSM resistance

3. Act. 3: Deploy insect and disease resistance genes
Methodology
• The experiments were conducted at three locations
• Areka
• Humbo
• Wondogenet
• Where BSM infestation is very common
• Four managed environments were created
– 1. Non stress
• Chemical treated for BSM and fully irrigated
– 2.Intermittent Drought Stressed (Humbo and
Wondogenet)
• Stressed before flower initiation and at pod initiation
– 3. Terminal drought stress at flower initiation (Humbo and
Wondogenet)
– 4. Terminal drought stress at pod initiation (Areka, Humbo
and Wondogenet)

4. Act. 3: Deploy insect and disease resistance genes
Methodology
 The materials evaluated in three groups
14 regionally and nationally released common bean varieties
82 RILs from cross G2333 x G19839 ( CIAT)
100 RILS from cross BAT881 x G21212 ( CIAT)
using
14*14 simple lattice design
Two rows plot leaving one row between plots
60 cm spacing between rows and 10 cm between
plants spacing

5. Act. 3: Deploy insect and disease resistance genes
Data collected
• All phenology data were collected
• BSM count per plant
• % of lodged plants due to BSM
• BSM damage score per plot
• BSM damage score per Individual plant
• SPAD reading
• Canopy temp
• Root pulling resistance
• Stem Diameter
• PHI
• Yield
• Pod wall thickness

6. Areka non-stress
Humbo non-stress
Humbo stress
Areka stress

7. G2333 x G19839 RIL
with adventitious root
BAT881 x G21212 RIL
Field performance of two RIL populations
under drought and BSM stress

8. BAT881
BAT881 x G21212 RIL
G19839
G21212
Grain yield kgha-1 Stress
G2333
Regional varieties
G19839 x G2333 RIL
Grain yield kgha-1 NS
Performance of RILs, parents and regional varieties for combined effect of DS & BSM
at Areka, Ethiopia 2011

9. Grain yield kgha-1 Stress
Grain yield kgha-1 NS
Performance of RILs, parents and regional varieties for combined effect of DS & BSM
at Humbo, Ethiopia 2011

10. Act. 4: Deploy drought tolerance traits in
Andean gene pool
Milestone 6: 36 Andean genotypes characterized
for drought traits
• 36 Andean genotypes were evaluate at Awassa
Ethiopia under irrigated and stressed conditions
and data on canopy temperature, root pulling
resistance, PHI and grain yield

11. Act. 4: Deploy drought tolerance traits in
Andean gene pool
Milestone 7: Three visual scales for drought
• 64 lines developed from non-wilting parents
were evaluated at Awassa Ethiopia for wilting
resistance, root pulling resistance and PHI

12. Act. 4: Deploy drought tolerance traits in
Andean gene pool
Milestone 11: 1 population (drought-tolerant Durango x Andean) of 190
lines phenotyped for MARS (years 2 and 3), in Colombia and
Ethiopia
– 126 DAB lines evaluated in Ethiopia at two sites under drought
stress and non-stress environments using 9 x 14 alpha lattice
design
– 72 SAB lines evaluated in Ethiopia at two sites under drought
stress and non-stress environments using 9 x 8 alpha lattice
• Population development and schematic diagram of MARS
• Population developed from single and multiple crosses
• Cross 16357 (CAL 143 x SAB 620) x SAB 626
• Cross 16358 (SAB 628 x CAL 143) x SAB 659
• Cross 16359 (SAB 628 x CAL 143) x SAB 686
• Advanced to F3-6 generation

13. Marker -assisted recurrent selection scheme on beans
Single and multiple cross
Cross 16357 (CAL 143 x SAB 620) x SAB 626
Cross 16358 (SAB 628 x CAL 143) x SAB 659
Cycle one Cross 16359 (SAB 628 x CAL 143) x SAB 686
DAB lines
F1
F2
Genotyping of F3-6 Pheotyping of the whole
parents (CIAT) population(Colombia
and Ethiopia)
Genotyping of the whole
population
(CIAT)
QTL

14. Cycle two Selection of the best lines
Genotyping of the
based on QTL results
selected genotypes
Intercross the best lines (at least
10 genotypes/family )
F2
Pheotyping of the
Genotyping of the
population population
(Colombia and Ethiopia)
(CIAT)
QTL
Development of the best lines

15. Description of phenoytping Locations
• Arbaminch
– is located in the Gamo Gofa Zone of the Southern Nations,
Nationalities, and Peoples Region about 500 kilometres south of Addis
Ababa, at an elevation of 1285 meters above sea level.
– Geographical coordinates: 6° 2' 0" North, 37° 33' 0" East.
– The soil is characterized in clay loam texture.
• Wondo genete
– is located in the Sidama Zone of the Southern Nations, Nationalities,
and Peoples Region about 300 kilometres South of Addis Ababa,
– at an elevation of 1760 meters above sea level.
– The Geographical coordinates: ??
– The soil is characterized in loam texture.

16. MARS population early growth

18. MARS population evaluated by
Professors from University of Free
State and Dr Beebe

19. Support from GCP
• Irrigation system

20. Support from GCP
• Technician training on farm machinery

21. Acknowledgments
• GCP
• CIAT
• Dr Steve Beebe
• Dr Hannibal M from AgriconInternational
• Jake Bentum
• Dr Bodo
• Gillian Summers
• SARI