International conference, CUI Vehari

1. Kinza Irshad, Malik Owais Ullah Awan,
Dr. Akhtar Iqbal, Dr. Bilal Ahmad Zafar Amin, Dr. Farhan Hafeez, Rashid Nazir
Department of Environmental Sciences, CUI, Abbottabad
International Conference on Environmental Toxicology and Health
Evaluation of novel lignocellulytic bacterial-fungal
consortia originated from Lower Himalaya,
for the excretion of degrading enzymes
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2. Introduction
First
Generation
Second
Generation
Soil and Ecosystem Ecology Lab, COMSATS University Islamabad, Abbottabad campus 2

3. Lignocellulosic Biomass
The biomass containing Lignin, cellulose and hemicellulose as the main constituent
Soil and Ecosystem Ecology Lab, COMSATS University Islamabad, Abbottabad campus 3

4. Lignocellulosic degradation
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Physical
Chemical
Fermentation
Biological
Bacteria and Fungi
Lignocellulytic
enzymes
Soil and Ecosystem Ecology Lab, COMSATS University Islamabad, Abbottabad campus 4
(Masran et al., 2016)

5. Lignocellulytic enzymes
– Various lytic-enzymes are produced by certain bacteria and fungi
– Considered as environmental friendly and sustainable for lignocellulosic
biomass degradation
– Can be used further for enzyme-cocktail production
Lignocellulosic degradation potential
Soil and Ecosystem Ecology Lab, COMSATS University Islamabad, Abbottabad campus 5

6. Lignocellulosic
Biomass
Himalayan Region,
Pakistan
Galiyat Forest
Study Area
Microbes
Soil and Ecosystem Ecology Lab, COMSATS University Islamabad, Abbottabad campus 6

7. Methodology
Decaying wood
Adjacent soil
Pine forest, Galyat
regon Lower Himalaya
Biochemical
characterization
Molecular
characterization
Soil and Ecosystem Ecology Lab, COMSATS University Islamabad, Abbottabad campus 7

9. Enzymatic assay of isolated Himalayan bacterial strains
Strain code
XylanaseUILaccaseUICellulaseUI
Soil and Ecosystem Ecology Lab, COMSATS University Islamabad, Abbottabad campus 9

10. Acknowledgments
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11. Thanks for your Attention
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