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LIGNOLYTIC FUNGI
LIGNIN DECOMPOSER IN SOIL
20282 – PDN
A. A. J. K. Eranga
What Is Ligninolytic Fungi?
• Ligninolytic fungi are taxonomically heterogeneous higher fungi
characterized by a unique ability to depolymerize & mineralize lignin.
• The extracellular, nonspecific, & oxidative
enzymatic system of these fungi catalyzes
lignin degradation
2
Why Lignolytics Are Important?
Lignolytic enzymes degrade many persistent aromatic compounds(PAH,PCB) with
structures similar to those of the metabolites formed in the biosynthesis or
degradation of lignin.
• process undergo - “enzymatic combustion”
The catalytic action increase the polarity & water-soluble products, which are more
accessible for both fungal metabolism and further degradation of natural soil micro
flora.
The unique properties of Ligninolytic fungi make them promising for use in
bioremediation, particularly if pollutants are difficult to decompose by bacteria.
3
Who Are Lignolytic Species?
• White-rot fungi (Basidiomycetes)
EX - Coriolus versicolor
Phanerochaete chrysosporium
Trametes versicolor
• Brown-rot fungi
EX - Coniophora puteana
• Soft rotter/litter decomposers
EX - Melanocarpus albomyces
Chaetomium thermophile
Agnaporthe grisea
Myrothecium verrucaria 4
Fig a
Fig b - Erwin, E. (2016)
- Darshan, M., et al. 2019
5
• Laccase is isoenzymes with monomeric or dimeric and glycosylation levels (10–45%).
This contains four copper ions of three different types.
4-benzenediol + O2 4-benzosemiquinone + 2H2O
• Lignin Peroxidase is a glycoprotein with high
molecular mass & contain one mole of iron
protoporphyrin IX per one mole of protein,
catalyzes the H2O2 dependent oxidative
depolymerization of lignin.
• Manganese Peroxidase is a glycosylated heme
protein, with high molecular mass &
depolymerizes the lignin molecule in the
presence of manganese ion.
• Versatile Peroxidase has broad range substrate
sharing traits while disclose typical features of
the manganese & lignin peroxidase families 6
 Laccase EC – 1.10.3.2
 Lignin peroxidase (LiP) - EC 1.11.1.14
 Manganese peroxidase (MnP) - EC 1.11.1.13
 Versatile peroxidase (VP) - EC1.11.1.16
Maciel et al., (2010)
7
Economical & environmental Importance
• Bioremediation
• Food industry
• Paper & pulp industry
• Textile industry
• Pharmaceutical & cosmetic
• Organic, medical &
• Nanotechnology applications 8
The most important role of Ligninolytic fungi in nature is to take contribution in the
global carbon cycle.
REFERENCES
1. Darshan M. R., Akshaya G., Assessment of white rot fungus mediated hardwood degradation by FTIR
spectroscopy & multivariate analysis, Journal of Microbiological Methods, Volume 157, 2019,Pages 123-
130.
2. Dashtban, M., Schraft, H., Syed, T. A., & Qin, W. (2010). Fungal biodegradation and enzymatic
modification of lignin. International journal of biochemistry and molecular biology, 1(1), 36–50.
3. Erwin, E. (2016). Microscopic decay pattern of yellow meranti (Shorea gibbosa) wood caused by white-rot
fungus Phlebia brevispora. Biodiversitas, 17, 417-421.
4. Maciel, M. J. M., Silva A. C., Ribeiro H. C. T., (2010). Industrial & biotechnological applications of
Ligninolytic enzymes of the Basidiomycota: A review. Electronic Journal of Biotechnology, vol13 issue 6
full text 2.
5. Novotný, C. & Svobodová, K. & Erbanová, P. & Cajthaml, T. & Kasinath, A. & Lang, E. & Šašek, V.. (2004).
Ligninolytic Fungi in Bioremediation: Extracellular Enzyme Production & Degradation Rate. Soil Biology
and Biochemistry. 36.
6. Plácido, J., Capareda, S. (2015). Ligninolytic enzymes: a biotechnological alternative for bioethanol
production. Bioresour. Bioprocess. 2, 23.
9
Thank you..!
10

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Lignolytic fungi, magical fungi, kingdom fungi/ soil enzyme generating fungi

  • 1. LIGNOLYTIC FUNGI LIGNIN DECOMPOSER IN SOIL 20282 – PDN A. A. J. K. Eranga
  • 2. What Is Ligninolytic Fungi? • Ligninolytic fungi are taxonomically heterogeneous higher fungi characterized by a unique ability to depolymerize & mineralize lignin. • The extracellular, nonspecific, & oxidative enzymatic system of these fungi catalyzes lignin degradation 2
  • 3. Why Lignolytics Are Important? Lignolytic enzymes degrade many persistent aromatic compounds(PAH,PCB) with structures similar to those of the metabolites formed in the biosynthesis or degradation of lignin. • process undergo - “enzymatic combustion” The catalytic action increase the polarity & water-soluble products, which are more accessible for both fungal metabolism and further degradation of natural soil micro flora. The unique properties of Ligninolytic fungi make them promising for use in bioremediation, particularly if pollutants are difficult to decompose by bacteria. 3
  • 4. Who Are Lignolytic Species? • White-rot fungi (Basidiomycetes) EX - Coriolus versicolor Phanerochaete chrysosporium Trametes versicolor • Brown-rot fungi EX - Coniophora puteana • Soft rotter/litter decomposers EX - Melanocarpus albomyces Chaetomium thermophile Agnaporthe grisea Myrothecium verrucaria 4
  • 5. Fig a Fig b - Erwin, E. (2016) - Darshan, M., et al. 2019 5
  • 6. • Laccase is isoenzymes with monomeric or dimeric and glycosylation levels (10–45%). This contains four copper ions of three different types. 4-benzenediol + O2 4-benzosemiquinone + 2H2O • Lignin Peroxidase is a glycoprotein with high molecular mass & contain one mole of iron protoporphyrin IX per one mole of protein, catalyzes the H2O2 dependent oxidative depolymerization of lignin. • Manganese Peroxidase is a glycosylated heme protein, with high molecular mass & depolymerizes the lignin molecule in the presence of manganese ion. • Versatile Peroxidase has broad range substrate sharing traits while disclose typical features of the manganese & lignin peroxidase families 6  Laccase EC – 1.10.3.2  Lignin peroxidase (LiP) - EC 1.11.1.14  Manganese peroxidase (MnP) - EC 1.11.1.13  Versatile peroxidase (VP) - EC1.11.1.16
  • 7. Maciel et al., (2010) 7
  • 8. Economical & environmental Importance • Bioremediation • Food industry • Paper & pulp industry • Textile industry • Pharmaceutical & cosmetic • Organic, medical & • Nanotechnology applications 8 The most important role of Ligninolytic fungi in nature is to take contribution in the global carbon cycle.
  • 9. REFERENCES 1. Darshan M. R., Akshaya G., Assessment of white rot fungus mediated hardwood degradation by FTIR spectroscopy & multivariate analysis, Journal of Microbiological Methods, Volume 157, 2019,Pages 123- 130. 2. Dashtban, M., Schraft, H., Syed, T. A., & Qin, W. (2010). Fungal biodegradation and enzymatic modification of lignin. International journal of biochemistry and molecular biology, 1(1), 36–50. 3. Erwin, E. (2016). Microscopic decay pattern of yellow meranti (Shorea gibbosa) wood caused by white-rot fungus Phlebia brevispora. Biodiversitas, 17, 417-421. 4. Maciel, M. J. M., Silva A. C., Ribeiro H. C. T., (2010). Industrial & biotechnological applications of Ligninolytic enzymes of the Basidiomycota: A review. Electronic Journal of Biotechnology, vol13 issue 6 full text 2. 5. Novotný, C. & Svobodová, K. & Erbanová, P. & Cajthaml, T. & Kasinath, A. & Lang, E. & Šašek, V.. (2004). Ligninolytic Fungi in Bioremediation: Extracellular Enzyme Production & Degradation Rate. Soil Biology and Biochemistry. 36. 6. Plácido, J., Capareda, S. (2015). Ligninolytic enzymes: a biotechnological alternative for bioethanol production. Bioresour. Bioprocess. 2, 23. 9