The document describes the light microscope. It defines a microscope as an optical instrument used to see small objects. It discusses the history of the microscope, including that the first compound microscope was created in 1590 and that Hooke and van Leeuwenhoek made early microscopic discoveries. It also outlines the basic parts of the light microscope, how they work together to magnify specimens, and proper use techniques.
Compound microscopes are what most people visualize when they think about microscopes. They are available in monocular, binocular and trinocular formats. They have a number of objectives (the lens closest to the object being viewed) of varying magnifications mounted in a rotating nosepiece.
This presentation talks about one of the most important technique in biology which is microscopy. The inclusions are the history of microscope, different types/kinds/classifications of microscope nowadays and the parts of a simple compound microscope
In the late 16th century several Dutch lens makers designed devices that magnified objects, but in 1609 Galileo Galilei perfected the first device known as a microscope. Dutch spectacle makers Zaccharias Janssen and Hans Lipperhey are noted as the first men to develop the concept of the compound microscope.
Compound microscopes are what most people visualize when they think about microscopes. They are available in monocular, binocular and trinocular formats. They have a number of objectives (the lens closest to the object being viewed) of varying magnifications mounted in a rotating nosepiece.
This presentation talks about one of the most important technique in biology which is microscopy. The inclusions are the history of microscope, different types/kinds/classifications of microscope nowadays and the parts of a simple compound microscope
In the late 16th century several Dutch lens makers designed devices that magnified objects, but in 1609 Galileo Galilei perfected the first device known as a microscope. Dutch spectacle makers Zaccharias Janssen and Hans Lipperhey are noted as the first men to develop the concept of the compound microscope.
Introduction
History
Compound microscope
Variants of microscopes
Dark field microscope
Phase contrast microscope
Fluorescent microscope
Polarising microscope
Electron microscope
A Microscope is an instrument for viewing objects that are too small to be seen by the naked/ unaided eyes.
In Greek micron= small
skopien=to look at
The science of investigating small object using such an instrument is called microscopy
The term microscopic means minute or very small, not visible with the eye unless aided by a microscope
From ancient times, man wanted to see things for smaller than could be perceived with the naked eye.
This led to the construction in the 16th century, of a magnifier composed of a single convex lens, and this in turn led to the eventual development of the microscope.
The most famous early pioneers in the history of microscope are Digges of England and Hans & Zcharias Janssen of Holland
It was Antony Van Leeuwenhoek who became the man to make and use a real microscope.
Leeuwenhoek microscope was called as single lens microscope because it had convex lens attached to metal holder and was focused using screws
Intro to the Microscope PPT FOR MBBS STUDENTSssuser77fe3b
A microscope is an instrument that is used to magnify small objects. Some microscopes can even be used to observe an object at the cellular level, allowing scientists to see the shape of a cell, its nucleus, mitochondria, and other organelles. While the modern microscope has many parts, the most important pieces are its lenses. It is through the microscope’s lenses that the image of an object can be magnified and observed in detail. A simple light microscope manipulates how light enters the eye using a convex lens, where both sides of the lens are curved outwards. When light reflects off of an object being viewed under the microscope and passes through the lens, it bends towards the eye. This makes the object look bigger than it actually is.
Over the course of the microscope’s history, hile some older microscopes had only one lens, modern microscopes make use of multiple lenses to enlarge an image. There are two sets of lenses in both the compound microscope and the dissecting microscope (also called the stereo microscope). Both of these microscopes have an objective lens, which is closer to the object, and an eyepiece, which is the lens you look through. The eyepiece lens typically magnifies an object to appear ten times its actual size, while the magnification of the objective lens can vary. Compound microscopes can have up to four objective lenses of different magnifications, and the microscope can be adjusted to choose the magnification that best suits the viewer’s needs. The total magnification that a certain combination of lenses provides is determined by multiplying the magnifications of the eyepiece and the objective lens being used. For example, if both the eyepiece and the objective lens magnify an object ten times, the object would appear one hundred tThe dissecting microscope provides a lower magnification than the compound microscope, but produces a three-dimensional image. This makes the dissecting microscope good for viewing objects that are larger than a few cells but too small to see in detail with the human eye. The compound microscope is typically used for observing objects at the cellular level.Over the course of the microscope’s history, technological innovations have made the microscope easier to use and have improved the quality of the images produced. The compound microscope, which consists of at least two lenses, was invented in 1590 by Dutch spectacle-makers Zacharias and Hans Jansen. Some of the earliest microscopes were also made by a Dutchman named Antoine Van Leeuwenhoek. Leeuwenhoek’s microscopes consisted of a small glass ball set inside a metal frame. He became known for using his microscopes to observe freshwater, single-celled microorganisms that he called “animalcules.”
While some older microscopes had only one loutwards. When light reflects off of an object being viewed under the microscope and passes through the lens, it bends towards the eye. This makes the object look bigger than it actually is.. Some of
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8. Types of Microscopes
• Light Microscopy
• Use of any kind of microscope that uses visible light to observe specimens
• Types of light microscopy
• Compound light microscopy
• Darkfield microscopy
• Phase-contrast microscopy
• Fluorescence microscopy
• Confocal microscopy
• Electronic Microscopes
9. The Compound Light Microscope
• In a compound microscope, the image from the objective lens is magnified again
by the ocular lens
• Total magnification is product of the magnification of its ocular and its objective
lenses
• Total magnification = objective lens ocular lens
• Resolution is the ability of the lenses to distinguish two points
10.
11. • Ocular lens ( eye piece):
The lens at the top that you look through .
They are usually 10x or 15x power
14. • Objective lenses :
Usually you find 3 or 4 objectives lenses on a microscope .They almost
always consist of 4x, 10x, 40, 100x powers .
When coupled with 10x ( most common ) eye lens , we get total
magnifications of 40 x, 100x, 400x and 1000x .
15. • The shortest objective lens is lowest power , the longest one is the lens with
greatest power .
• Lenses are color coded :
Red : 4x
Yellow : 10x ( low power objective)
Blue : 40x ( high power objective)
White : 100x
20. • Base :
The bottom of the microscope ,used for support
21. Steps to Use:
1. Rotate the low power objective into place and make sure the
stage is all the way down.
2. Place slide on stage making sure object to be viewed is
centered over the hole in the stage. Use the stage clips to
hold the slide in place.
3. Turn light on.
4. Focus first with the coarse adjustment knob. Once in focus on
low power, turn the nosepiece until the next higher lens is in
place.
5. Use FINE adjustment knob ONLY and focus the object.
22. • Always carry with 2 hands
• Never touch the lenses with your fingers.
• Only use lens paper for cleaning
• Keep objects clear of desk and cords
• When you are finished with your "scope", rotate
the nosepiece so that it's on the low power
objective, roll the stage down to lowest level,
rubber band the cord, then replace the dust
cover.
