This document provides an overview of microscopy, including:
1. It defines microscopy as using an instrument called a microscope to view objects too small to see with the naked eye.
2. It describes some key parts and types of microscopes like compound, phase contrast, dark ground, and electron microscopes.
3. It explains concepts like magnification, resolution, and aberration that are important for microscopy.
Introduction to microscopy
Different parts of a microscope & their function
Different types of microscopy
Different types of optical microscopy
Different types of electron microscopy
Different terms used in microscopy
Staining- Simple, Differential, Special
Gram Staining
Introduction
History
Compound microscope
Variants of microscopes
Dark field microscope
Phase contrast microscope
Fluorescent microscope
Polarising microscope
Electron microscope
A Microscope is an instrument for viewing objects that are too small to be seen by the naked/ unaided eyes.
In Greek micron= small
skopien=to look at
The science of investigating small object using such an instrument is called microscopy
The term microscopic means minute or very small, not visible with the eye unless aided by a microscope
From ancient times, man wanted to see things for smaller than could be perceived with the naked eye.
This led to the construction in the 16th century, of a magnifier composed of a single convex lens, and this in turn led to the eventual development of the microscope.
The most famous early pioneers in the history of microscope are Digges of England and Hans & Zcharias Janssen of Holland
It was Antony Van Leeuwenhoek who became the man to make and use a real microscope.
Leeuwenhoek microscope was called as single lens microscope because it had convex lens attached to metal holder and was focused using screws
Introduction to microscopy
Different parts of a microscope & their function
Different types of microscopy
Different types of optical microscopy
Different types of electron microscopy
Different terms used in microscopy
Staining- Simple, Differential, Special
Gram Staining
Introduction
History
Compound microscope
Variants of microscopes
Dark field microscope
Phase contrast microscope
Fluorescent microscope
Polarising microscope
Electron microscope
A Microscope is an instrument for viewing objects that are too small to be seen by the naked/ unaided eyes.
In Greek micron= small
skopien=to look at
The science of investigating small object using such an instrument is called microscopy
The term microscopic means minute or very small, not visible with the eye unless aided by a microscope
From ancient times, man wanted to see things for smaller than could be perceived with the naked eye.
This led to the construction in the 16th century, of a magnifier composed of a single convex lens, and this in turn led to the eventual development of the microscope.
The most famous early pioneers in the history of microscope are Digges of England and Hans & Zcharias Janssen of Holland
It was Antony Van Leeuwenhoek who became the man to make and use a real microscope.
Leeuwenhoek microscope was called as single lens microscope because it had convex lens attached to metal holder and was focused using screws
In the late 16th century several Dutch lens makers designed devices that magnified objects, but in 1609 Galileo Galilei perfected the first device known as a microscope. Dutch spectacle makers Zaccharias Janssen and Hans Lipperhey are noted as the first men to develop the concept of the compound microscope.
These slide provide the information about history of microscopy and scientists whose contribute in the growth of microscopy
microscopy Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye).
A microscope is an instrument used to see objects that are too small to be seen by the naked eye.
In the late 16th century several Dutch lens makers designed devices that magnified objects, but in 1609 Galileo Galilei perfected the first device known as a microscope. Dutch spectacle makers Zaccharias Janssen and Hans Lipperhey are noted as the first men to develop the concept of the compound microscope.
These slide provide the information about history of microscopy and scientists whose contribute in the growth of microscopy
microscopy Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye).
A microscope is an instrument used to see objects that are too small to be seen by the naked eye.
Lab Report: Isolation of Pure Culture, Gram-staining, and Microscopic Observa...Annisa Hayatunnufus
A Lab Report under the subject of Microbiology. Done as a lab session in Josai University, Japan during a twinning program on 2014.
Created by: Annisa Hayatunnufus
Bachelor of Pharmacy
Management & Science University
as a partial requirement for one of my subject for this semester
I would like you to view my presentation and comment as well
I will be very glad if you find my presentation interesting, or comment on how I can improve my craft, THANK YOU :)
in this ppt we discuss about article topic which is aimportant topic of english grammmer and manish kumar created this presentation,who is owner of http://exclusivestoke.com
The microscope has evolved a lot from the time of Leeuwenhoek. This presentation gives a brief overview about the types of microscope their principle of function and application.
INTRODUCTION
HISTORY
TYPES OF MICROSCOPE
Compound Microscope
Dissection Microscope
Scanning Electron Microscope (SEM)
Transmission Electron Microscope (TEM
CARE
PARTS AND FUNCTION
FOCUSING
CONCLUSION
REFERANCE
This presentation talks about one of the most important technique in biology which is microscopy. The inclusions are the history of microscope, different types/kinds/classifications of microscope nowadays and the parts of a simple compound microscope
This ppt contain all about Bright field simple microscope.
Here You can find uses, parts and principle and limitation of Bright field simple microscope.
you can also understand the difference between bright field simple microscope and bright field compound microscope.
11. LR = 0.61 x W W = Wavelength NA = Num aperture NA Types of microscope Resolving power Compound Microscope 200 nanometers Scanning Electron Microscope 10 nanometers Transmission Electron Microscope 0.2 nanometers
22. What’s the power of this lens? To calculate the power of magnification, multiply the power of the ocular lens by the power of the objective , e..g.: 10x40=400 times What are the powers of magnification for each of the objectives we have on our microscopes? Fill in the table on your worksheet.
23.
24. Comparing Powers of Magnification We can see better details with higher the powers of magnification, but we cannot see as much of the image. 10x 40x Which of these images would be viewed at a higher power of magnification?
25. Let’s give it a try ... 1 – Turn on the microscope and then rotate the nosepiece to click the red-banded objective into place. 2 – Place a slide on the stage and secure it using the stage clips. Use the coarse adjustment knob (large knob) to get it the image into view and then use the fine adjustment knob (small knob) to make it clearer. 4 – When you are done, turn off the microscope and put up the slides you used. 3 – Once you have the image in view, rotate the nosepiece to view it under different powers. Draw what you see on your worksheet! Be careful with the largest objective! Sometimes there is not enough room and you will not be able to use it!
26. How to make a wet-mount slide … 1 – Get a clean slide and coverslip from your teacher. 2 – Place ONE drop of water in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess! 3 – Place the edge of the cover slip on one side of the water drop. You do not need to use the stage clips when viewing wet-mount slides! 5 – Place the slide on the stage and view it first with the red-banded objective. Once you see the image, you can rotate the nosepiece to view the slide with the different objectives. 4 - Slowly lower the cover slip on top of the drop. Cover Slip Lower slowly
28. Multiple step operation employed to attain optimal illumination: 1. Remove any diffusing filter. 2. Put a slide on the stage and focus. 3. Completely close the field diaphragm. 4. Move the condenser until the border or the iris hexagon is neat and clear. 5. Center if necessary. 6. Open the field diaphragm until the tip of the hexagon touches the field limit KOHLER’S ILLUMINATION
30. The Parts of a Microscope Body Tube Objective Lenses Stage Clips Diaphragm Light Source Ocular Lens Arm Stage Coarse Adj Fine Adjustment Base Nose Piece
78. - Scan a gold-plated specimen to give a 3-D view of the surface of an object which is black and white. - Used to study surface features of cells and viruses. - Scanning Electron microscope has resolution 1000 times better than Light microscope . Scanning Electron Microscope
Hans Janssen and his son Zacharias Janssen, Dutch spectacle-makers developed first microscope.
Slide should be scanned in an orderly fashion so that vital information is not missed.
A number of errors may be encountered while performing microscopy. Some of the common cause of errors in focusing is enlisted below.
The condenser annulus or annular diaphragm is opaque flat-black (light absorbing) plate with a transparent annular ring. which is positioned in the front focal plane (aperture) of the condenser so the specimen can be illuminated by defocused, parallel light wave fronts emanating from the ring .
Phase contrast enables internal cellular components, such as the membrane, nuclei, mitochondria, spindles, mitotic apparatus, chromosomes, Golgi . widely employed in diagnosis of tumor cells .and the growth, dynamics, and behavior of a wide variety of living cells in culture tissue culture investigations. apparatus, and cytoplasmic granules from both plant and animal cells and tissues to be readily visualized