This document provides an overview of infrared (IR) spectroscopy. It discusses the basic principles of IR spectroscopy, including molecular vibrations and instrumentation. The major components of IR spectrometers are described, such as IR radiation sources, wavelength selectors, sample handling techniques, detectors, and recorders. Factors that can affect vibrational frequencies are outlined. Finally, applications of IR spectroscopy in fields like pharmaceutical analysis are mentioned.
This presentation is about the monochromators and specifically their use in spectroscopy. It includes definition, principle, origin of term, principle, types, prism monochromator, diffraction grating monochromator, difference in both of them, their uses and working as well, optical filters and their uses and application.
A Monochromator is an optical device that transmits a mechanically selectable narrow band of wavelengths of light chosen from a wider range of wavelengths available at the input. And the unwanted radiations are blocked by the slit allowing only the desired ray to pass (monochromatic).
A dispersive element disperse the polychromatic light into several bands of single wavelength and then a slit is used which stops the unwanted bands of light, allowing only the desired monochromatic light to pass through its exit point.
By fixing the slit and rotating the dispersive element, the direction of the dispersed light is turned so that the colour of the resulting monochromatic light changes.
When electromagnetic radiation passes through a prism, it is refracted because the index of refraction of the prism material is different from that of air.
Shorter wavelengths are refracted more than longer wavelengths.
By rotation of the prism, different wavelengths of the spectrum can be made to pass through an exit slit and through the sample.
A prism works satisfactorily in the ultraviolet and visible regions and can also be used in the infrared region.
Because of its nonlinear dispersion, it works more effectively for the shorter wavelengths.
Glass prisms and lenses can be used in the visible region.
Quartz or fused silica must be used in the ultraviolet region.
The entire monochromatic compartment must be kept dry.
In this slides contains principle and instrumentation of Differential Scanning Calorimeter (DSC).
Presented by: N Poojitha. (Department of pharmaceutics),
RIPER, anantapur.
Its a Perfect Powerpoint Presentation For Bachelors and Masters Of Chemistry Students. It Covers All the Basic Portion and Syllabus Which you Want in a Presentation. So,Go For It Friends!!
An Infrared spectrum represents a fingerprint of a sample with absorption peaks which correspond to the frequencies of vibrations between the bonds of the atoms making up the material-Because each different material is a unique combination of atoms, no two compounds produce the exact same spectrum, therefore IR can result in a unique identification of every different kind of material!
Infrared spectroscopy is technique to identify the functional group of the molecule.
In Infrared spectroscopy there are two main region finger print region and functional group region. Most of the molecules identifies In the finger print region due to that it is complex region.
Now we will see the
principle of IR spectroscopy:
IR spectroscopy is vibrational energy level changes when IR radiation passes through the material.
In this slide contains instrumentation of Fourier-Transform Nuclear Magnetic Resonance (FT-NMR).
Presented by: P. Venkatesh. (Department of pharmaceutical analysis).
RIPER, anantpur.
This presentation is about the monochromators and specifically their use in spectroscopy. It includes definition, principle, origin of term, principle, types, prism monochromator, diffraction grating monochromator, difference in both of them, their uses and working as well, optical filters and their uses and application.
A Monochromator is an optical device that transmits a mechanically selectable narrow band of wavelengths of light chosen from a wider range of wavelengths available at the input. And the unwanted radiations are blocked by the slit allowing only the desired ray to pass (monochromatic).
A dispersive element disperse the polychromatic light into several bands of single wavelength and then a slit is used which stops the unwanted bands of light, allowing only the desired monochromatic light to pass through its exit point.
By fixing the slit and rotating the dispersive element, the direction of the dispersed light is turned so that the colour of the resulting monochromatic light changes.
When electromagnetic radiation passes through a prism, it is refracted because the index of refraction of the prism material is different from that of air.
Shorter wavelengths are refracted more than longer wavelengths.
By rotation of the prism, different wavelengths of the spectrum can be made to pass through an exit slit and through the sample.
A prism works satisfactorily in the ultraviolet and visible regions and can also be used in the infrared region.
Because of its nonlinear dispersion, it works more effectively for the shorter wavelengths.
Glass prisms and lenses can be used in the visible region.
Quartz or fused silica must be used in the ultraviolet region.
The entire monochromatic compartment must be kept dry.
In this slides contains principle and instrumentation of Differential Scanning Calorimeter (DSC).
Presented by: N Poojitha. (Department of pharmaceutics),
RIPER, anantapur.
Its a Perfect Powerpoint Presentation For Bachelors and Masters Of Chemistry Students. It Covers All the Basic Portion and Syllabus Which you Want in a Presentation. So,Go For It Friends!!
An Infrared spectrum represents a fingerprint of a sample with absorption peaks which correspond to the frequencies of vibrations between the bonds of the atoms making up the material-Because each different material is a unique combination of atoms, no two compounds produce the exact same spectrum, therefore IR can result in a unique identification of every different kind of material!
Infrared spectroscopy is technique to identify the functional group of the molecule.
In Infrared spectroscopy there are two main region finger print region and functional group region. Most of the molecules identifies In the finger print region due to that it is complex region.
Now we will see the
principle of IR spectroscopy:
IR spectroscopy is vibrational energy level changes when IR radiation passes through the material.
In this slide contains instrumentation of Fourier-Transform Nuclear Magnetic Resonance (FT-NMR).
Presented by: P. Venkatesh. (Department of pharmaceutical analysis).
RIPER, anantpur.
IR SPECTROSCOPY-INTRODUCTION, PRINCIPLE, TYPE OF VIBRATIONS, INSTRUMENTATION, APPLICATION{ FOR the m.pharm 1st year 2019
Presented by DIPSANKAR BERA(M.PHARM STUDENT)
Seminar on Uv Visible spectroscopy by Amogh G VAmoghGV
PPT of seminar on UV Visible spectroscopy, electronic transitions, Instrumentation of Double beam spectrophotometers, Advantages of Double beam over single beam, Beer Lamberts law derivation
Niosomes-Method of preparation,evaluation,applications.pptxDipeshGamare
Niosomes the novel carriers for targeted drug delivery for various disease condition. This powerpoint presentation covers various methods of preparation, evaluation and application of niosomes.
Theories of dispersion, pharmaceutical dispersion (Emulsion and suspension).pptxDipeshGamare
In this presentation Theories of dispersion, pharmaceutical dispersion (Emulsion and suspension) with their mechanism, methods of preparation and stability studies are mentioned.
SELF-EMULSIFYING DRUG DELIVERY SYSTEM (SEDDS).pptxDipeshGamare
SELF-EMULSIFYING DRUG DELIVERY SYSTEM (SEDDS) is a type of novel drug delivery system, in this presentation all aspect regarding SEDDS are covered with some novel points.
A radiopharmaceutical is a radioactive compound (radioisotopes and molecules labelled with radioisotopes) used for the diagnosis and therapeutic treatment of human diseases.
Therapeutic Drug Monitoring (TDM) involves the analysis, assessment, and evaluation of circulating concentrations of drugs in serum, plasma, or whole blood.
Transfer from R & D to production.pptxDipeshGamare
Transfer from R & D to production :
(Process, packaging and cleaning)
Granularity of TT Process :
(API, excipients, finished products, packaging materials)
Introduction and official standards for sieves, standard for sieves and dimensions and notations, materials used for sieve, punch plates, modes of motion in size separation.
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
Acute scrotum is a general term referring to an emergency condition affecting the contents or the wall of the scrotum.
There are a number of conditions that present acutely, predominantly with pain and/or swelling
A careful and detailed history and examination, and in some cases, investigations allow differentiation between these diagnoses. A prompt diagnosis is essential as the patient may require urgent surgical intervention
Testicular torsion refers to twisting of the spermatic cord, causing ischaemia of the testicle.
Testicular torsion results from inadequate fixation of the testis to the tunica vaginalis producing ischemia from reduced arterial inflow and venous outflow obstruction.
The prevalence of testicular torsion in adult patients hospitalized with acute scrotal pain is approximately 25 to 50 percent
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
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TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
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Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
Knee anatomy and clinical tests 2024.pdfvimalpl1234
This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
2. CONTENT
Introduction to IR spectroscopy
Modes of molecular vibration
Sample handling
Instrumentation of Dispersive-IR and FTIR
Factors affecting vibrational frequencies
Applications of IR spectroscopy
References
2
4. IR SPECTROCOPY:
Infrared (IR) spectroscopy is based on IR absorption by molecules as undergo vibrational and rotational
transitions.
The IR spectrum is mainly used to determine the functional groups present in a compound and elucidate the
structure of compound.
The energy of a molecule in IR = Electronic energy + Vibrational energy + Rotational energy
4
5. 1.Near IR : carbohydrates and
proteins
2.Middle IR : organic molecules -
functional groups
3.Far IR : inorganic co-ordination
bonds& quaternary ammonium
compounds
REGION WAVE
LENGTH
λ (μm)
WAVE NUMBER
υ (cm-1)
FREQUENCY
Hz
RANGE
NEAR 0.78 - 2.5 12800 - 4000 3.8x1014 -1.2 x1014
MIDDLE 2.5 - 50 4000 - 200 1.2x1014 – 6 x1012
FAR 50 - 1000 200 -10 6x1012 – 3 x1011
MOST USED 2.5 - 15 4000- 670 1.2x1014 - 2 x1013
Different compounds when absorb infrared radiation give rise to vibrational transitions characteristic to the
molecular structure and those transitions are recorded as IR Spectra.
Mid IR region is widely used in pharmaceutical studies.
A compound can be analyzed by IR radiation only if:
1. There is a change in dipole movement.
2. There should be a resonance in applied and natural IR frequency.
When we apply an external infrared frequency, and it matches the natural frequency of vibration within a
molecule, the applied energy is then absorbed by the molecule and an infrared peak is observed. These peaks
are characteristic to different functional group and parts of the molecule, and they are unique for every
molecule. Hence the IR spectroscopy is considered as the fingerprint analysis of a molecule.
PRINCIPLE OF IR SPECTROSCOPY
5
6. Fingerprint region (400-1500cm−1): This region of IR spectrum provides characteristic peaks for different part of a
molecule and helps in qualitative identification of compounds.
Group frequency region (4000—1500cm−1): Peaks with respect to different functional groups are observed in this
region.
Characteristic IR frequencies of some known
functional groups
Typical IR spectrum depicting regions of
absorption
6
7. Example : The spectrum is for a substance with an empirical formula of C3H5N. What is the compound?
Nitrile or
alkyne
group
No
aromatics
Aliphatic
hydrogens
One or
more alkane
groups
Propane nitrile
7
9. Fundamental vibrations
1. Bond Stretching vibration: In stretching vibration, the length of a bond is changed i.e. either increased or decreased.
It is of two types:
A] Symmetrical stretching: Here the two bonds increase or decreases in length in a symmetric pattern.
B] Asymmetrical stretching: Here when one bond length increases the other one decreases.
2. Bending vibration:
Bending vibrations are of two sub-types.
1) In plane
A] Rocking : The atoms swing back and forth concerning the central atom of a molecule Two hydrogen atoms of a methylene
molecule move outward simultaneously and inward simultaneously
B] Scissoring : The two atoms approach each other and go away from each other.
2) Out of the plane
A] Wagging : Such type of vibration occurs when both the atoms of a molecule move one side of the plane
B] Twisting : In twisting, one molecule is above the plane and the other molecule is below the plane.
Non-fundamental vibrations:
1. Overtone vibrations occur at twice or thrice the value of fundamental vibrations.
2. Combination bands are the bands that result because of coupling of fundamental frequencies.
3. Fermi resonance bands are produced because of coupling between fundamental vibration and an overtone or because of a
combination band.
9
10. SAMPLE HANDLING
The sample cells are usually made up of transparent ionic substances such as sodium chloride (NaCl) or potassium bromide (KBr).
However, KBr is preferred because of its compatibility with the instrumental measurement process. The infrared samples can be
considered of three forms such as:
1) Liquids sampling :
A drop of liquid is placed within the KBr/ NaCl plates with
the thickness less than 0.01 mm.
Around 1-10mg of sample is required to prepare the
solution.
Aqueous solutions are not preferred as they tend to dissolve
the sample cells so, the solvent should be anhydrous and
mostly organic solvents such as chloroform is preferred.
2) Solid sampling : Pelletization technique
The solid samples can be prepared using pressed pellet
technique where the sample is placed in sample holder and
KBr pellets are prepared in form of thin transparent layer
using a hydraulic press.
However, as KBr is hygroscopic in nature care should be
taken to minimize its atmospheric exposure.
10
11. 3) Solution sampling technique
To prepare a solid solution 1 to 10 mg volumes of 0.1 to 1.0 ml of 0.05 to 10% solution are required for placement within a
cell of 0.1 to 1mm thickness.
Solvents used are CHCl₃, CS2 and CCl₄ is used for this purpose which absorbs strongly at 785 cm-1.
Care should be taken to avoid solution combinations that react instantaneously.
4) Semisolid sample preparation
Another technique called as Nujol-Mull technique (popularly called as Mull Technique) may be used where the mixing up of
the sample with a mineral oil (Nujol) ( liquid paraffin hydrocarbon) is done and afterwards a thin film of the liquid can be
applied on the liquid sample cell for measurements.
5)Gas sampling
Gas samples are enclosed in a gas cell with windows, which seal the gas cell and allow the infrared beam to pass into the cell.
These cells are made up of KBr, NaCl and so on. These cells are about 10cm long and can increased its path length upto 40cm
by multiple reflections inside it and gas sample can be analyzed easily.
11
12. INSTRUMENTATION
The instrumentation of IR spectrophotometer is as follows
1) IR radiation source
2) Monochromators / wavelength selector
3) Sample holder
4) Detector
5) Recorder
Diagrammatic representation of Dispersive-IR spectrophotometer Diagrammatic representation of Fourier Transorm-IR spectrophotometer
12
14. 1) IR radiation source :
a) Nernst Glower
Rare earth metal oxides (Zr, Ce, Th) heated electrically.
Apply current to cylinder, has resistance to current flow generates heat (1200o – 2200o C).
Causes light production similar to blackbody radiation.
Range of use ~ 670 – 10,000cm-1.
Need good current control or overheats and damaged.
b) Globar
Similar to Nernst Glower but uses silicon carbide rod instead of rare earth oxides
Similar usable range
V
Zr, Ce, Th
14
15. c) Incandescent Wire Source
• Tightly wound nichrome or rhodium wire that is electrically heated.
• Same principal as Nernst Glower.
• Lower intensity then Nernst Glower or Globar, but longer lifetime.
d) CO2 Laser
• CO2 laser gas mixture consists of 70% He, 15% CO2, and 15% N2 .
• A voltage is placed across the gas, exciting N2 to lowest vibrational levels.
• The excited N2 populate the asymmetric vibrational states in the CO2 through collisions.
• Infrared output of the laser is the result of transitions between rotational states of the CO2 molecule .
• Gives off band of ~ 100 cm-1 in range of 900-1100 cm-1 .
• Small range but can choose which band used & many compounds have IR absorbance in this region.
• Much more intense than Blackbody sources.
e) Others
1) Mercury arc (far IR) – when continues radiation source is required.
2) Tungsten lamp (4000 -12,800cm-1) (near IR)
15
17. 2) WAVELENGTH SELECTORS / MONOCHROMATORS
They help in selecting a continuous IR radiation in the desired wavelength region.
They generally contain a chopper and a complex system of monochromators.
The choppers are generally used to enhance the signal to noise ratio of the instrument, and they also moderate
the intensity of radiation that reaches to the detector.
The monochromator is used to select it desired frequency of radiation which will be then allowed to incident on
the detector.
These monochromators are of two types:
1. Prismatic monochromator: They are made up of
glass/quartz and coated with alkyl halides.
A] Mono Pass (Radiation passes only once through the prism)
B] Double Pass (Radiation passes twice through the prism)
2. Grating monochromator: They are grooves made up of
aluminium and provide better dispersion of radiation than prisms.
A] Reflection gratings
B] Transmittance grating
17
18. 3) SAMPLE HOLDER/SAMPLE CELL
• There is rugged window material for cuvette or sample holder which is transparent and also inert over this region.
• AgCl cell are used for aqueous and moist samples, but it is soft and easily gets deformed and darkens on exposure to visible
light.
• The alkali halides are widely used, particularly NaCl, KBr and ThBr which is transparent at wavelength as long as 625 cm-1.
KBr pellet (For solid sample)
NaCl windows ( For liquid sample)
4) DETECTOR
1. Thermocouple
2. Bolometer
3. Photoconducting cell
4. Pyroelectric detector
5. Golay cell
6. Thermistor
18
19. 1.Thermocouple
Two pieces of dissimilar metals fused together at the ends.
When heated, metals heat at different rates.
Potential difference is created between two metals that varies with their difference in temperature
Usually made with blackened surface (to improve heat absorption)
Placed in evacuated tube with window transparent to IR (not glass or quartz)
IR “hits” and heats one of the two wires.
Can use several thermocouples to increase sensitivity.
2. Bolometer
Strips of metal (Pt, Ni) or semiconductor that has a large change in resistance to current with temperature.
As light is absorbed by blackened surface, resistance increases and current decreases.
Very sensitive.
3. Photoconducting cell
Thin film of semiconductor (ex. PbS) on a nonconducting glass surface and sealed in a vacuum.
Absorption of light by semiconductor moves from non-conducting to conducting state.
Decrease in resistance increase in current.
Range: 10,000 -333 cm-1 at room temperature.
V
- +
hn metal1 metal2
IR transparent
material (NaCl)
A
hn
glass
semiconductor
hn
vacuum
Transparent
to IR
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20. 4. Pyroelectric Detectors
Pyroelectric (ceramic, lithium tantalate) material get polarized (separation of (+) and (-) charges) in
presence of electric field.
Temperature dependent polarization
Measure degree of polarization related to temperature of crystal
Fast response, good for FTIR
5. Golay cell
It contains a small metal cylinder which is closed by black metal plate at end and a flexible metal die
programmed at the other end.
The cylinder is filled with xenon and sealed.
When IR radiation is allowed to fall on the black metal plate it heats the gas which expands it.
The signal detected by the photo tube is then modulated according to the power of radiant beam.
6. Thermistor
Thermistors are usually made up of fused mixture of metallic oxides.
As the temperature of the mixture increases its electrical resistance decreases and accordingly the
measurements are performed in the higher spectrophotometer.
5) RECORDER OR READ OUT UNIT
The signals collected from detector are amplify and recorded by device in terms of IR spectra
indicating various peaks of functional group present.
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21. FACTORS AFFECTING VIBRATIONAL FREQUENCIES
A] Coupled vibration
An isolated C-H bond has only one stretching vibration frequency whereas methylene group shows two stretching vibrations
both symmetrical and asymmetrical.
Because of mechanical coupling or interaction between CH stretching vibrations in the CH2 group.
Asymmetric vibrations develop at higher frequency or wave numbers than stretching vibrations.
Such vibrations are called coupled vibrations because these vibrations occur at different frequency then the required for an
isolated CH stretching
B] Fermi resonance
Coupling of two fundamental vibration modes produces 2 new modes of vibration.
With frequencies higher and lower than the observed in absence of interaction.
This interaction takes place between fundamental vibrations and overtones, or combination tone vibration and they are
collectively called us Fermi resonance.
In Fermi resonance molecule transfer energy from fundamental vibrations into overtone or combination bands.
The resonance pushes the two levels apart and mixes their character and each level has some amount of fundamental and some
amount of overtone or combination tone characters.
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22. FACTORS AFFECTING VIBRATIONAL FREQUENCIES
C] Electronic effects
Changing the absorption frequencies for a particular group takes place when the substituent in the neighborhood of the group
is changed.
They include
Inductive effect: Inclusion of alkyl group gives +ve Inductive effect producing decreased absorption frequency. Adding an
electronegative atom or group produces –ve inductive effect leading to increased absorption frequency.
Mesomeric effect: It produces lengthening or weakening of a bond leading to lowering of absorption frequency.
Field effect: In ortho substituted compounds, the lone pair of electrons on two atoms influence each other through space
interactions and change the vibrational frequencies of both the groups. Example: Ortho halo acetophenone. This is called field
effect.
D] Hydrogen bonding
It occurs in any system having a proton donor group and a proton acceptor. If the S orbital of the proton effectively overlaps
the P or 𝜋 orbital of the acceptor group.
The stronger the hydrogen bond, the longer the OH bond, the lower the vibration frequency, broader and more intense will be
the absorption.
Intermolecular bonds produce broad bands whereas intramolecular hydrogen bonds produce sharp and well-defined bands.
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23. IR spectroscopy is applied for qualitative as well as quantitative analysis of drugs in pharmaceutical industry.
It is used for identification of drug substances
It identifies the impurities present in a drug sample
It helps in study of hydrogen bonding both intermolecular and intramolecular type.
It is widely used in study of polymers.
It elucidates reaction mechanisms.
It identifies functional groups present in any sample.
It estimates stability of formulation.
It distinguishes between Cis and Trans isomers.
It can predict the keto-enol tautomerism.
APPLICATIONS OF IR SPECTROSCOPY
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24. 1. Chatwal, G.R. and Anand, S.K.J. (2018) Instrumental Methods of Chemical Analysis. Himalaya Publishing House, Mumbai,
2.3-2.82.
2. Marcelli, A., Cricenti, A., Kwiatek, W. M., & Petibois, C. (2012). Biological applications of synchrotron radiation infrared
spectromicroscopy. Biotechnology Advances, 30(6), 1390–1404. https://doi.org/10.1016/j.biotechadv.2012.02.012.
3. Basa’ar, O., Fatema, S., Alrabie, A., Mohsin, M., & Farooqui, M. (2017). Supercritical carbon dioxide extraction of Triognella
foenum graecum Linn seeds: Determination of bioactive compounds and pharmacological analysis. Asian Pacific Journal of
Tropical Biomedicine, 7(12), 1085–1091. https://doi.org/10.1016/j.apjtb.2017.10.010
4. IRTracer-100. (2022). https://www.shimadzu.com/an/products/molecular-spectroscopy/ftir/ftir-spectroscopy/irtracer-
100/index.html
5. Jasco. (2020, October 6). Principles of infrared spectroscopy (1) Molecular vibrations and infrared absorption | JASCO
Global. JASCO Inc. https://www.jasco-global.com/principle/principles-of-infrared-spectroscopy-1-molecular-vibrations-and-
infrared-absorption/
6. Libretexts. (2020, August 5) Information Obtained from IR Spectra. Chemistry LibreTexts.
https://chem.libretexts.org/Ancillary_Materials/Laboratory_Experiments/Wet_Lab_Experiments/Organic_Chemistry_Labs/La
b_I/10%3A_Infrared_Spectroscopy/10.06%3A_Information_Obtained_from_IR_Spectra
REFERENCES
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