In this slides contains principle and instrumentation of Differential Scanning Calorimeter (DSC).
Presented by: N Poojitha. (Department of pharmaceutics),
RIPER, anantapur.
An Infrared spectrum represents a fingerprint of a sample with absorption peaks which correspond to the frequencies of vibrations between the bonds of the atoms making up the material-Because each different material is a unique combination of atoms, no two compounds produce the exact same spectrum, therefore IR can result in a unique identification of every different kind of material!
In this slide contains Interference In Atomic Absorption Spectroscopy and applications.
Presented by: Shaik Gouse ul azam. ( department of pharmaceutical analysis.)
RIPER, anantpur.
IR SPECTROSCOPY, INTRODUCTION, PRINCIPLE, THEORY, FATE OF ABSORBED RADIATION, FERMI RESONANCE, FINGERPRINT REGION, VIBRATIONS, FACTORS AFFECTING ABSORPTION OF IR RADIATION, SAMPLING TECHNIQUES, APPLICATIONS OF IR SPECTROSCOPY.
Slide covers three methods of thermal analysis i.e., thermogravimetry, differential thermal analysis, and differential scanning calorimetry. Thermal analysis methods are well-established techniques in research laboratories of pharmaceutical industry. Thermal analysis includes all methods measuring some parameter during the heating of a sample .Thermal analysis is widely used to study the thermal stability, char content, and decomposition temperature of polymer composites reinforced with natural/synthetic fibers/or nanosized fillers etc.
In DSC the heat flow is measured and plotted against temperature of furnace or time to get a thermo gram. This is the basis of Differential Scanning Calorimetry (DSC).
The deviation observed above the base (zero) line is called exothermic transition and below is called endothermic transition.
In this slides contains Differential Thermal analysis (DTA) and Differential Scanning calorimetry (DSC).
Presented by : J.Vinay Krishna (Department of industrial pharmacy).
RIPER, anantapur.
In this slide contains principle, description of Differential Thermal analysis Application in Polymers.
Presented by: RAMY SALIHEEN (Department of pharmaceutics).
RIPER, anantapur
An Infrared spectrum represents a fingerprint of a sample with absorption peaks which correspond to the frequencies of vibrations between the bonds of the atoms making up the material-Because each different material is a unique combination of atoms, no two compounds produce the exact same spectrum, therefore IR can result in a unique identification of every different kind of material!
In this slide contains Interference In Atomic Absorption Spectroscopy and applications.
Presented by: Shaik Gouse ul azam. ( department of pharmaceutical analysis.)
RIPER, anantpur.
IR SPECTROSCOPY, INTRODUCTION, PRINCIPLE, THEORY, FATE OF ABSORBED RADIATION, FERMI RESONANCE, FINGERPRINT REGION, VIBRATIONS, FACTORS AFFECTING ABSORPTION OF IR RADIATION, SAMPLING TECHNIQUES, APPLICATIONS OF IR SPECTROSCOPY.
Slide covers three methods of thermal analysis i.e., thermogravimetry, differential thermal analysis, and differential scanning calorimetry. Thermal analysis methods are well-established techniques in research laboratories of pharmaceutical industry. Thermal analysis includes all methods measuring some parameter during the heating of a sample .Thermal analysis is widely used to study the thermal stability, char content, and decomposition temperature of polymer composites reinforced with natural/synthetic fibers/or nanosized fillers etc.
In DSC the heat flow is measured and plotted against temperature of furnace or time to get a thermo gram. This is the basis of Differential Scanning Calorimetry (DSC).
The deviation observed above the base (zero) line is called exothermic transition and below is called endothermic transition.
In this slides contains Differential Thermal analysis (DTA) and Differential Scanning calorimetry (DSC).
Presented by : J.Vinay Krishna (Department of industrial pharmacy).
RIPER, anantapur.
In this slide contains principle, description of Differential Thermal analysis Application in Polymers.
Presented by: RAMY SALIHEEN (Department of pharmaceutics).
RIPER, anantapur
In this slide contains principle, types, materials used, factors affecting gel electrophoresis.
Presented by: I. Sai Reddemma (Department of pharmacology).
RIPER, anantapur.
In this slide contains definition, validation method of HVAC
Presented by: V NABI RASOOL (Department of pharmaceutical analysis and quality assurance).RIPER, anantapur.
In this slide contains types, working principle, factors affecting, advantage and disadvantage of paper electrophoresis.
Presented by: G.Sai Swetha. (Department of pharmacology),
RIPER, anantapur.
In this slide contains the deep explanation of Methods of Determination for Drug-Excipient Compatibility Studies.
Presented by: G.Aravind Kumar (Department of industrial pharmacy),
RIPER, anantapur.
Introduction to PCR and RT-PCR, RT-PCR
PCR, Contents of PCR, Steps in PCR, PCR VS RT-PCR, Applications
Presented by
N. Ramya
Department of Pharmacology
In this slide contains Factors Affecting Resolution In HPLC and its criteria's.
Presented by: M.Sudheeshna. (Department of pharmaceutical analysis).
RIPER,anantpur.
In this slide contains introduction, principle and applications of differential scanning colorimetry.
Presented by: G.Kavya (Department of pharmaceutics)
RIPER,anantapur.
In this slide contains instrumentation of Fourier-Transform Nuclear Magnetic Resonance (FT-NMR).
Presented by: P. Venkatesh. (Department of pharmaceutical analysis).
RIPER, anantpur.
Introduction to Analytical Techniques in Phaese III,
Spectrophotometry, Reflectance photometry, Nephelometry & Turbidimetry, Osmometry, Potentiometry, Flowcytometry, Densitometry, Electrophoresis, LC-MS, ICP-MS
Presented by
B. Kranthi Kumar
Department of Pharmacology
In this slide contains analytical techniques in phase-3 clinical trials.
Presented by: KRANTHI KUMAR BONALA (Department of pharmacology).
RIPER, anantapur
in this slides contains principle and types of detectors used in Gas Chromatography.
Presented by: J.Vinay Krishna. (Department of industrial pharmacy),
RIPER, anantapur.
In this slide contains contents, steps, different and application of PCR and RT-PCR
Presented by: RAMYA NAGARAJU GARI (Department of pharmacology).
RIPER, anantapur
In this slide contains principle, advantage, dis advantage and application of UPLC.
Presented by: P. Sudheer Kumar. (Department of pharmaceutical analysis)
RIPER, anantapur.
Similar to Differential Scanning Calorimeter Instrumentation.(DSC) (20)
JOURNAL CLUB PRESENTATION (20L81S0402-PA & QA)
Presented by: K VENKATSAI PRASAD (Department of pharmaceutical analysis and quality assurance).RIPER, anantapur
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
1. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 1
Differential Scanning Calorimeter
Instrumentation
A Seminar as a part of curricular requirement
for I year M. Pharm I semester
Presented by
N. Poojitha
(Reg. No. 20L81S0308)
Under the guidance
Dr. Nawaz Mahammad
M. Pharm, Ph.D
Associate Professor
Dept. of Pharmaceutics
2. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 2
• Introduction of DSC
• Principle of DSC
• Instrumentation of DSC
• References
Contents
3. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 3
Introduction of DSC
• The technique was developed by E.S. Watson and M.J. O Neill in
1960, and introduced commercially at the Pittsburgh Conference on
Analytical Chemistry and Applied Spectroscopy in 1963.
• This technique is used to study what happens to polymers/samples
upon heating.
• It is used to study thermal transitions of a polymer/sample (the
changes that take place on heating)
• The differential scanning calorimeter does all of the above functions
and heats the sample with the linear temperature.
• Both the sample and reference are maintained at nearly the same
temperature through out the experiment in DSC
4. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 4
Principle of DSC
• It is a technique in which the energy necessary to establish a zero
temperature difference between the sample & reference material is
measured as a function of temperature.
• Here, sample & reference material are heated by separate heaters in
such a way that their temp are kept equal while these temp. are
increased or decreased linearly.
• During heating two types of reactions can be take place one is the
endothermic and the other is the exothermic.
5. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 5
Endothermic reaction:
• If sample absorbs some amount of heat during phase transition then
reaction is said to be endothermic.
• In endothermic reaction more energy needed to maintain zero temp
difference between sample & reference.
• E.g. Melting, boiling, sublimation, vaporization
6. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 6
Exothermic reaction:
• If sample released some amount of heat during phase transition,
then reaction is said to be exothermic.
• In exothermic reaction, less energy needed to maintain zero temp
difference between sample & reference.
• E.g crystallization, degradation, polymerization
7. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 7
DSC Graph
8. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 8
DSC Instrumentation
9. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 9
• Two basic types of DSC instruments: Heat flux DSC, Power
compensated DSC
Types of DSC Instuments
10. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 10
1.Heat-flux DSC
• Sample Holder :
PLATINUM, ALUMINIUM, STAINLESS STEEL
• SENSORS:
Temperature Sensors
Usually thermocouples, which are same for the sample are reference
• Furnace:
One block for both sample and reference cells.
• Temperature:
The temperature difference between the sample and reference is
converted to differential thermal power, which is supplied to the heaters
to maintain the temperature of the sample and reference at program
value.
11. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 11
2.Power Compensated DSC
• Sample Holder :
PLATINUM, ALUMINIUM, STAINLESS STEEL PANS
• SENSORS:
Pt resistance thermocouple
Separate sensors and heaters for the sample and reference sample.
• Furnace:
Separate blocks for sample and reference cells.
• Temperature:
Differential thermal power is supplied to the heaters to maintain the
temperature of the sample and reference at the program level.
12. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 12
DSC attached to Computer
13. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 13
• P. J. Elving & I. M. Kolthoff, Chemical analysis, Vol. 19, P134, 1964.
• Wang L, Wang B, Lin Q. Demonstration of MEMS-based differential
scanning calorimetry for determining thermodynamic properties of
biomolecules. Sensor Actuat B-Chem 2008;134:953– 958
• Masri MS, Reuter FW, Fiedman M. Interaction of wool with metal
cations. J Appl Polym Sci 1974;18:675–681
• Hohne G, Hemminger W, Flammersheim H-J. Differential Scanning
Calimetry: An Introduction for Practitioners. Berlin, Germany:
Springer-Verlag, 1996 .
• Instrumental methods of analysis- Willards,7th edition, CBS
Publications ;237-332. 11/12/2019
References
14. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 14