BIOTECHNOLOGY IS CHALLENGING SUBJECT TO TEACH AND UNDERSTAND ALSO .....THEIR INTERESTING PART IS TO LEARN ABOUT IMMUNITY AND THE IMPORTANT PART MAJOR COMPATIBILITY COMPLEX
BP-605T, Pharmaceutical biotechnology, Structure of immunoglobulins, classification of immunoglobulins, explanation of structure of immunoglobulin, digestion with proteolytic enzymes, Fab region, Fc region, role of different immunoglobulin classes, structure of IGM, IGA, IGG, IGE, IGD, Light chain, heavy chain, kappa, lambda, papain enzyme, pepsin enzyme
Immunity is the balanced state of multicellular organisms having adequate biological defenses to fight infection, disease, or other unwanted biological invasion, while having adequate tolerance to avoid allergy, and autoimmune diseases.
BP-605T, Pharmaceutical biotechnology, Structure of immunoglobulins, classification of immunoglobulins, explanation of structure of immunoglobulin, digestion with proteolytic enzymes, Fab region, Fc region, role of different immunoglobulin classes, structure of IGM, IGA, IGG, IGE, IGD, Light chain, heavy chain, kappa, lambda, papain enzyme, pepsin enzyme
Immunity is the balanced state of multicellular organisms having adequate biological defenses to fight infection, disease, or other unwanted biological invasion, while having adequate tolerance to avoid allergy, and autoimmune diseases.
PREPARATION OF BACTERIAL VACCINES:
Steps involved in killed bacterial vaccine preparation:
1. Selection of an antigen:
The exact strain or strains to be incorporated for preparation of bacterial vaccine.
Eg. Cholera vaccine: smooth strains of the two serological types Inaba and Ogawa
TABC vaccine: O and H antigens in S. typhi and S. paratyphi microorganisms and these organisms also contains Vi antigen.
Each strain is carefully checked for freedom from variation and absence of contaminating organisms.
Immunity is the ability of the body to protect against all types of foreign bodies like bacteria, virus, toxic substances etc. As it protects us from diseases it is also called disease resistance. Immunity is done by immune system which is a complex network of lymphoid organs such as bone marrow, thymus, spleen etc. It is mainly of two types- Natural and Acquired immunity.
General method of the preparation of Bacterial Infections, Toxoids, Viral vaccine, Antitoxins, Serum-immune blood derivatives and other products relative to immunity.
Antibiotics are common agents used in modern healthcare. This was not always the case. From ancient times, people sought ways to treat those who were afflicted with infections. Dyes, molds, and even heavy metals were thought to hold promise for healing.[1] Various microorganisms hold medical significance, including bacteria, viruses, fungi, and parasites. Antibiotics are compounds that target bacteria and thus, are intended to treat and prevent bacterial infections.
PREPARATION OF BACTERIAL VACCINES:
Steps involved in killed bacterial vaccine preparation:
1. Selection of an antigen:
The exact strain or strains to be incorporated for preparation of bacterial vaccine.
Eg. Cholera vaccine: smooth strains of the two serological types Inaba and Ogawa
TABC vaccine: O and H antigens in S. typhi and S. paratyphi microorganisms and these organisms also contains Vi antigen.
Each strain is carefully checked for freedom from variation and absence of contaminating organisms.
Immunity is the ability of the body to protect against all types of foreign bodies like bacteria, virus, toxic substances etc. As it protects us from diseases it is also called disease resistance. Immunity is done by immune system which is a complex network of lymphoid organs such as bone marrow, thymus, spleen etc. It is mainly of two types- Natural and Acquired immunity.
General method of the preparation of Bacterial Infections, Toxoids, Viral vaccine, Antitoxins, Serum-immune blood derivatives and other products relative to immunity.
Antibiotics are common agents used in modern healthcare. This was not always the case. From ancient times, people sought ways to treat those who were afflicted with infections. Dyes, molds, and even heavy metals were thought to hold promise for healing.[1] Various microorganisms hold medical significance, including bacteria, viruses, fungi, and parasites. Antibiotics are compounds that target bacteria and thus, are intended to treat and prevent bacterial infections.
B pharma
D pharma
Pharmaceutical Biotechnology
Pharmaceutics I
Immunity and Immunological Products
types of immunity
Immunology
Toxins antibody exotoxins endotoxins
Vaccine
toxoids
sera
B.C.G. vaccine.
cholera. pertussis, plague and typhoid vaccine.
typhus vaccine.
measles, small-pox. poliomyelitis and yellow fever.
diphtheria, tetanus and staphylococcus.
Diagnostic preparations containing bacterial toxins used for Schick test and tuberculin test.
Preparations containing antibodies (antiserum, and antitoxins)used to produce passive immunity
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This is the process by which individual’s immune system becomes fortified against an infectious agents (immunogen) to a subsequent encounter naturally or , also achieved by vaccination.
An exaggerated immune response to innocuous antigens (harmless foreign substance) in an individual upon reexposure. Also known as Hypersensitivity or Allergic reactions.
This ppt contains all the information about the Immunity - Host defences. It is useful for students of the medical field learning Preventive and social medicine, Swasthavritta (Ayurved), and everyone who is interested in knowing about it
BOTECHNOLOGY IS CHALLENGING SUBJECT TO TEACH AND UNDERSTAND ALSO .....THEIR INTERESTING PART IS TO LEARN ABOUT PRODUCTION OF CITRIC ACID , PENICILLIN, GLUTAMIC ACID , GRISIOFULVIN , VITAMIN B 12
BOTECHNOLOGY IS CHALLENGING SUBJECT TO TEACH AND UNDERSTAND ALSO .....THEIR INTERESTING PART IS TO LEARN ABOUT MICROBIAL BIO TRANSFORMATION WITH BIOCHEMICAL REACTIONS
BIOTECHNOLOGY IS CHALLENGING SUBJECT TO TEACH AND UNDERSTAND ALSO .....THEIR INTERESTING PART IS TO LEARN ABOUT IMMUNITY AND THE IMPORTANT PART MAJOR COMPATIBILITY COMPLEX
OTECHNOLOGY IS CHALLENGING SUBJECT TO TEACH AND UNDERSTAND ALSO .....THEIR INTERESTING PART IS TO LEARN ABOUT MICROBIAL GENETICS AND THEIR METHODS OF GENE TRANSFER
BIOTECHNOLOGY IS
CHALLENGING SUBJECT TO TEACH AND UNDERSTAND ......
ITS A VERY INTERESTING TO LEARN ABOUT HYBRIDOMA TECHNOLOGY .. THEIR PRODUCTION AND
APPLICATION ALSO ....
Biotechnology is challenging subject to teach and understand also..its a very interesting subject in pharmacy..all the power point is made as per your syllabus with point to point discussion.
Biotechnology is challenging subject to teach and understand also..its a very interesting subject in pharmacy..all the power point is made as per your syllabus with point to point discussion.
thank you
Navigating Challenges: Mental Health, Legislation, and the Prison System in B...Guillermo Rivera
This conference will delve into the intricate intersections between mental health, legal frameworks, and the prison system in Bolivia. It aims to provide a comprehensive overview of the current challenges faced by mental health professionals working within the legislative and correctional landscapes. Topics of discussion will include the prevalence and impact of mental health issues among the incarcerated population, the effectiveness of existing mental health policies and legislation, and potential reforms to enhance the mental health support system within prisons.
Leading the Way in Nephrology: Dr. David Greene's Work with Stem Cells for Ki...Dr. David Greene Arizona
As we watch Dr. Greene's continued efforts and research in Arizona, it's clear that stem cell therapy holds a promising key to unlocking new doors in the treatment of kidney disease. With each study and trial, we step closer to a world where kidney disease is no longer a life sentence but a treatable condition, thanks to pioneers like Dr. David Greene.
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Defecation
Normal defecation begins with movement in the left colon, moving stool toward the anus. When stool reaches the rectum, the distention causes relaxation of the internal sphincter and an awareness of the need to defecate. At the time of defecation, the external sphincter relaxes, and abdominal muscles contract, increasing intrarectal pressure and forcing the stool out
The Valsalva maneuver exerts pressure to expel faeces through a voluntary contraction of the abdominal muscles while maintaining forced expiration against a closed airway. Patients with cardiovascular disease, glaucoma, increased intracranial pressure, or a new surgical wound are at greater risk for cardiac dysrhythmias and elevated blood pressure with the Valsalva maneuver and need to avoid straining to pass the stool.
Normal defecation is painless, resulting in passage of soft, formed stool
CONSTIPATION
Constipation is a symptom, not a disease. Improper diet, reduced fluid intake, lack of exercise, and certain medications can cause constipation. For example, patients receiving opiates for pain after surgery often require a stool softener or laxative to prevent constipation. The signs of constipation include infrequent bowel movements (less than every 3 days), difficulty passing stools, excessive straining, inability to defecate at will, and hard feaces
IMPACTION
Fecal impaction results from unrelieved constipation. It is a collection of hardened feces wedged in the rectum that a person cannot expel. In cases of severe impaction the mass extends up into the sigmoid colon.
DIARRHEA
Diarrhea is an increase in the number of stools and the passage of liquid, unformed feces. It is associated with disorders affecting digestion, absorption, and secretion in the GI tract. Intestinal contents pass through the small and large intestine too quickly to allow for the usual absorption of fluid and nutrients. Irritation within the colon results in increased mucus secretion. As a result, feces become watery, and the patient is unable to control the urge to defecate. Normally an anal bag is safe and effective in long-term treatment of patients with fecal incontinence at home, in hospice, or in the hospital. Fecal incontinence is expensive and a potentially dangerous condition in terms of contamination and risk of skin ulceration
HEMORRHOIDS
Hemorrhoids are dilated, engorged veins in the lining of the rectum. They are either external or internal.
FLATULENCE
As gas accumulates in the lumen of the intestines, the bowel wall stretches and distends (flatulence). It is a common cause of abdominal fullness, pain, and cramping. Normally intestinal gas escapes through the mouth (belching) or the anus (passing of flatus)
FECAL INCONTINENCE
Fecal incontinence is the inability to control passage of feces and gas from the anus. Incontinence harms a patient’s body image
PREPARATION AND GIVING OF LAXATIVESACCORDING TO POTTER AND PERRY,
An enema is the instillation of a solution into the rectum and sig
2. IMMUNITY: DEFINITION
Immunity is the ability of the body to protect against all
types of foreign bodies like bacteria, virus, toxic
substances which enter the body.
Immunity is also called disease resistance.
The lack of immunity is known as susceptibility.
Immunity is derived from Latin word “immune” which
means free from burden.
In this case burden refers to disease caused by
microorganisms or their toxic products.
3. ANTIGEN AND ANTIBODY
Antigens –
Any substance which invokes an immunological
response is an antigen or immunogen .
Certain component of cell membrane act as a antigens.
Most are proteins or large polysaccharides from a foreign
organism.
Antibodies –
Proteins that recognize and bind to particular antigen
with very high specificity.
4. TYPES OF IMMUNITY
Any immune response or immunity involves initial
recognition of pathogen/foreign material and then a
reaction to eliminate it.
• Broadly, immunity falls into two categories:
1. Innate (inborn) immunity
a. Species immunity
b. b. Racial immunity
2. Adaptive (acquired) immunity
a. Active immunity
b. b. Passive immunity
5. INNATE ACQUIRED
ACTIVE (OWN
ANTIBODIES )
PASSIVE
(READYMADE
ANTIBODIES )
ARTIFICIAL
(IMMUNIZATIO
N )
NATURAL
(EXPOSURE TO
INFECTIOUS
AGENT)
NATURAL
(MATERNAL
ANTIBODIES )
ARTIFICIAL
(ANTIBODIES
FROM OTHER
SOURCESES )
IMMUNITY
6. 1. INNATE (NON SPECIFIC OR NATURAL)
IMMUNITY
It refers to the inborn-ability of the body to resist, and is
genetically transmitted from one generation to the next.
This immunity offers resistance to any microorganism or
foreign material encountered by the host.
Innate immunity lacks immunological memory, i.e., it
occurs to the same extent each time a microorganism or
foreign material is encountered.
Innate immunity can be divided into species, racial, and
individual immunity
7. A. SPECIES IMMUNITY
Species immunity (species resistance) is that in
which a disease affecting one species does not
affect the other species.
For convenience, humans do not contract cattle
plague, chicken cholera, hog cholera, infectious
horse anemia, etc.,
while animals are not affected by many human
diseases such as enteric fever, scarlet fever,
syphilis, gonorrhea, measles, etc.
Diseases of skin, to which humans are quite
susceptible, are often resisted by animals because
they have more hair and thicker hides.
8. B. RACIAL IMMUNITY
Racial immunity (racial resistance) is that in which various races
(breeds) (Breed is group of animals of the same species)show
marked differences in their resistance to certain infectious diseases.
A well known example is that Brahman cattle are resistant to the
protozoan parasite responsible for tick fever in other breeds of
cattle.
Similarly, Black Africans affected by sickle cell anaemia, a genetic
disease, are resistant to malaria while malaria affects other human
races.
Racial immunity (racial resistance) is that in which various races
(breeds) show marked differences in their resistance to certain
infectious diseases.
9. A well known example is that Brahman cattle are
resistant to the protozoan parasite responsible for tick
fever in other breeds of cattle.
Similarly, Black Africans affected by sickle cell anemia,
a genetic disease, are resistant to malaria while malaria
affects other human races.
10. C. INDIVIDUAL IMMUNITY
Having the same racial background and opportunity
for exposure, some individuals of the race
experience fewer or less severe infections than
other individuals of the same race.
For convenience, children are more susceptible to
diseases such as measles and chicken pox, while
aged individuals are susceptible to other diseases
like pneumonia.
11. 2. ACQUIRED (SPECIFIC OR ADAPTIVE)
IMMUNITY
It refers to an immunity that is developed by the
host in its body after exposure to a suitable antigen
or after transfer of antibodies or lymphocytes from
an immune donor.
Acquired immunity is highly adaptive and is capable
of specifically recognizing and selectively
eliminating foreign microorganisms and
macromolecules, i.e., antigens.
It exhibits the following four characteristic features
that distinguish it from nonspecific (innate)
immunity:
12. TYPES OF ACQUIRED IMMUNITY
Acquired immunity can be obtained by the host actively or
passively and, on this basis, can be categorized as of two
types:
a. Active immunity
The active immunity is the direct response of your body
against the pathogens.
It is induced by the exposure to a foreign antigen such as
the antigen of microbes.
It is an adaptive response of the individual after contact
with specific pathogen or antigen.
In active immunity, there is active involvement of host’s own
immune system leading to the synthesis of antibodies
and/or the production of immuno-competent cells (ICCs).
13. B. PASSIVE IMMUNITY
• The passive immunity is the immunity conferred to
an individual by the transfer of serum or
lymphocytes from a specifically immunized
individual.
• Passive immunity is a useful method for conferring
resistance without waiting for the development of
the active immune response.
• In passive immunity, on the contrary, the antibodies
and /or the immuno-competent cells (ICCs) are
transferred from one host to another
14. SR
NO
Active Immunity Passive Immunity
1 Produced actively by the immune
system of the host.
Produced passively by the immune
system of the host
2 Antibody production is induced by the
infection or by immunogens
Antibodies are not produced, but
directly transferred
3 Active immunity involves both cell
mediated and humoral immunity.
Passive immunity is due to the
presence of ready-made antibodies.
4 Natural active immunity is by clinical
infection
Natural passive immunity is by the
transfer of antibodies through placenta
5 Artificial active immunity is induced
by vaccination
Artificial passive immunity is induced
by injection of antibodies
6 Active immunity is durable Passive immunity is only transient
7 Active immunity offers effective
protection against microbes.
Passive immunity is less efficient in
offering complete protection
8 Immunological memory is present Immunological memory
9 In active immunity, the subsequent
doses with the antigen causes
booster effect
In passive immunity, the subsequent
doses is less effective due to the
immune elimination
10 Negative phase may occurs Negative phase is absent
15. Similarities between Active Immunity and Passive
Immunity
1. Both active and passive immunity are acquired
immunities
2. Both can be natural and artificial
3. Both types of immunity involve lymphocytes
4. The antimicrobial components in both the systems are
antibodies
5. Both are induced by the antigens
6. Both systems are specific
16. SR
NO
INNATE IMMUNITY ADAPTIVE IMMUNITY
1. Known as natural immunity Known as acquired immunity
2 Generate a non-specific immune
response
Generate a specific immune response
3 Always present in the body Generated in response to exposure to
an external factor
4 Generates a rapid response Delayed 5-6 days
5 Plasma proteins, phagocytes,
physical and chemical barriers are
the components
Humoral and cell mediated immunity
are the components
6 Temperature, pH, skin and mucous
membrane are the barriers
Lymph nodes, spleen and lymphoid
tissues are the barriers
7 Does not develop memory cells Develops memory cells
8 Possesses a less diversity, Less
potent
Possesses a higher diversity, Exhibits
a higher potency
9 Example: Redness and swelling
caused by the white blood cells
around a wound
Ex: Vaccination against a virus
17. CELLULAR AND HUMORAL IMMUNITY
The lymphocytes is the basic cell responsible for
the both cellular and humoral immunity.
Lymphocytes are found in high concentration in
lymph nodes , spleen , at the site they are
manufactured and processed(bone marrow and
thymus) .
They are classified as
Primary or Central lymphoid organs –
Bone marrow and thymus .
Secondary or peripheral lymphoid organs –
Lump node, spleen, adenoids, tonsils, appendix
etc.
18. Many lymphocytes pass through the thymus and
processed by hormonal microenvironment.
The lymphocytes are now called thymus derived
lymphocytes or T-Lymphocytes.
The bone marrow derived lymphocytes do not
enter and processed by the thymus are called
B- cells.
B- lymphocytes respond to antigen while T-
lymphocytes are responsible for cell mediated
immunity.
19. DIFFERENCE BETWEEN T-CELLS AND B- CELLS
Sr no Characteristics T-cells B- cells
1 Site of production Thymus Bursal
equivalent
tissue
2 Types of immunity Cell mediated Humoral
(antibody
mediated )
3 Secretory product Lymphokines Antibodies
4 Sub population Helper , killer cells Plasma cell ,
memory cells
5 Effect Against intracellular
viruses, fungi,
protozoa and cancer
cells
Against bacteria
, viruses present
in body fluids
6 Type of response Cytotoxic Precipitation ,
neutralization
20. The amino-terminal
portions, corresponding to
the V regions,
bind to antigen;
effector functions are
mediated by the other
domains)
VL = Variable region on
light
chain,
VH = variable region on
heavy chain,
CH = constant region on
heavy chain,
CL = Constant
region on light chain.
Structure of immunoglobulin's
21. STRUCTURE OF IMMUNOGLOBULIN'S
Immunoglobulin's are heterodimers containing four peptide
chains as their basic unit.
They are composed of two identical light chains (23kD) and
two identical heavy chains (50-70kD).
Each light chain is bound to a heavy chain by a disulfide
bond, and by noncovalent interactions as salt linkages,
hydrogen bonds, and hydrophobic bonds, to form a
heterodimer (H-L).
Similar noncovalent interactions and disulfide bridges link the
two identical heavy and light (H-L) chain combinations to each
other to form the basic four-chain (H-L)2 antibody structure, a
dimer of dimers.
When the amino acid sequences of many different heavy
chains and light chains were compared, it became clear that
both the heavy and light chain could be divided into two
regions based on variability in the amino acid sequences.
22. STRUCTURE OF IMMUNOGLOBULIN'S
All Immunoglobulin molecules basically consist of
Two identical heavy chains and
Two identical light chains
Held together by disulfide linkage (Inter chain and Intra
chain) and non- covalent interactions.
It is Y shaped tetramer (H2L2).
Each heavy chains contains 450 amino acids light
chains has 212 amino acids.
23. IMMUNOGLOBULIN'S
Specialized group of protein mostly associated with Y
globulin fraction.
Immunoglobulin is a functional term while Y globulin is a
physical term.
Abbreviated as Ig.
Ig are produced by plasma cells in response to an antigen
and which function as antibodies.
24. VARIABLE AND CONSTANT REGION –
Each chain of Ig has two region constant and
variable region.
Light chain –
Amino terminal is variable region (VL)
Carboxy terminal is constant region (CL)
Heavy chain –
One quarter of amino terminal region is variable
region (VH).
Remaining three quarters are constant region
(CH1) , (CH2), (CH3).
25. Amino acid sequence of variable regions light and
heavy chains is responsible for specific binding of
immunoglobulin (antibody ) with antigen .
There are certain hypervariale region mixed
between relatively invariable region.
Light chain has 3 hypervariable regions
Heavy chain has 4.
The hypervariable region more specifically
determine the antigen- antibody binding .
26. IMMUNOGLOBULIN CLASSES
Ig G gamma heavy chain
Ig M mu heavy chain
Ig A Alpha heavy chain
Ig D Delta heavy chain
Ig E Epsilon heavy chain
Two types of light chain kappa (k )and lambda.
Ig contains two kappa and 2 lambda light chains
Kappa chain is 60 % is more common in human.
27. Ig G –
Major serum Ig is 70 – 80 %.
Single Y shaped unit (manomer ).
It is antibody seen in secondary immuno response
It can transverse or cross blood vessels readily .
Ig G is only Ig that can cross the placenta and transfer the
mothers immunity to developing fetus.
IgG helps in bacterial immobilization.
IgG neutralize toxin and viruses.
Ig M –
It is largest Ig composed of 5 Y shaped units helds together
by J polypeptide chain.
Due to large size Ig M can not cross blood vessels hence
restricted to the blood stream.
Ig M is first antibody to be produced in response to antigen
and most effective against microorganism.
28. IG A –
Single (manomer) or double unit (diamer ) held together
by J chain .
Mostly found in body secretions such as saliva , tears
sweat , milk, and wall of intestine .
Ig A binds with bacterial antigen present on body surface
and remove them .
So Ig A prevents the foreign substances from entering the
body cell.
Ig E –
Single Y shaped manomer
Normally present in minute concentration in blood 0.3gm /
ml .
Ig E levels are elevated in individuals with allergies like
hay fever , asthma.
29. IG D
Ig D is single Y shaped manomer .
Present in low concentration in circulation .
Present on surface on B cells.
Their function is not known.
30.
31. MAJOR HISTOCOMPATIBILITY COMPLEX
(MHC)
Group of genes that code for proteins found on the surfaces
of cells that help the immune system recognize foreign
substances.
MHC proteins are found in all higher vertebrates.
In human beings the complex is also called the human leukocyte
antigen (HLA) system.
Major histocompatibility complex (MHC) is the cluster of gene
arranged within a long continuous stretch of DNA on
chromosome number 6 in Human which encodes MHC
molecules.
MHC molecule is a cell surface glycoprotein receptor present in
APCs and acts as antigen presenting structure It plays vital role
in immune recognition, including interaction between T cells and
other cell types.
In Human MHC is known as Human Leukocyte antigen (HLA)
complex and the genes of MHC are recognized in three classes,
consequently there are three types of MHC molecules.
32. TYPES OF MHC:
Class I MHC
Class II MHC
Class III MHC
MHC class-I:
Class-I MHC gene encodes glycoprotein molecule
which expressed on the surface of all nucleated
cells and platelets.
MHC-I molecule contains a 45KDa α-chain
associated non-covalentely with a 12KDa β2
microglobulin molecule.
Association of α-chain and β2 microglobulin is
required for expression of class-I MHC molecule
33.
34. Α-CHAIN OF MHC-I:
The α-chain is a transmembrane glycoprotein encoded by
polymorphic gene within A, B and C region of Human HLA
complex
The α-chain is anchored in the plasma membrane by its
hydrophobic trans-membrane segment and hydrophilic
cytoplasmic tail.
α-chain is made up of 3 domains (α1,α2 and α3). Each
domain containing approximately 90 amino acids, a
transmsmbrane domain of about 25 hydrophobic amino
acids followed by short stretch of charged (hydrophilic)
amino acids of cytoplasmic tails of 3o amino acids.
It can binds antigen of 8-10 animo acids long.
α3 and β2 are organized into β-pleated sheets, each formed
by antiparallel β-strand of amino acids, this structure is
known as immunoglobulin fold.
Because of this structure α-chain and β2 microglobulin are
classified as member of immunoglobulin super-family
receptor.
35. Β2 MICROGLOBULIN OF MHC-I
β2 microglobulin is a protein encoded by a highly
conserved gene located on different chromosome
β2 microglobulin is similar in size and organization to α3
domain.
Β2 microglobulin does not contain transmembrane
region and is non-covalently linked with α-chain.
Functions of MHC class I:
Major function of MHC-I is to bind peptide antigens and
present to CD8+ T cells (T helper cells)
CD8 T cells are specific for MHC-I antigen
MHC-I binds endogenous antigen and present to T
helper cells.
MHC-I molecules are found on surface of all nucleated
cells.
36. MHC CLASS-II:
Class-II MHC is the glycoprotein molecule expressed
primarily on antigen presenting cells such as macrophages,
dendritic cells and B-cells.
MHC-II molecules contains two different polypeptide chains,
1 33 KDa α-chain and 28KDa β-chain which are associated
by non-covalent interactions.
α-chain and β-chain of MHC-II:
α-chain and β-chain of MHC-II is a membrane bound
glycoprotein that contains external domains,
atransmembrane segment and acytoplasmic tail.
α-chain and β-chain are made up of two domains (α1 and
α2) and (β1 and β2) respectively.
The peptide biding cleft is a open ended groove formed
between α-chain and β-chain at proximal end. The cleft can
bind antigenic peptide of 13-18 aminoacids long.
37. Functions of MHC class II:
Major function of MHC-II is to bind peptide antigen
and present to CD4 T cells.
MHC-II are found on surface of Antigen presenting
cells (APCs).
CD4+T-cells are specific for MHC-II
Activates B cells for antibody production
MHC-II plays a significant role in graft versus host
response and in mixed lymphocyte reaction (MLR)
because the immune response gene is identical to
MHC-II in human.
38. MHC CLASS-III:
MHC-III are diverse group of molecules that serves
a wide variety of functions in immune system.
MHC-III are not a marker on cell surface.
Functions of MHC class-III:
Involved in complement activation
Involved in inflammation caused by cytokines,
tumor necrosis factors etc