Electrophoresis is a technique used to separate charged biomolecules like proteins and nucleic acids. It works by applying an electrical current to move these molecules through a buffer or gel based on their size and charge. Hemoglobin electrophoresis specifically separates different types of hemoglobin in the blood based on their charge. This test can detect abnormalities associated with conditions like sickle cell disease or thalassemias by identifying abnormal levels or types of hemoglobin present. The document provides details on how hemoglobin electrophoresis works, the normal hemoglobin types found, common abnormal types, and how the test is performed and interpreted.
challenges in interpreting abnormal hemoglobin study- the key is to correlate with patient age, ethnicity,RBC indices & morphology findings. Two tier approach for correct characterization of abnormal hemoglobins of HPLC &/or capillary electrophoresis.
I have listed out the LE cells structure and Microscopical examinaton of LE CELLS, Difference between tart cells and le cells, clinical symptoms and diagnostic procedure.
challenges in interpreting abnormal hemoglobin study- the key is to correlate with patient age, ethnicity,RBC indices & morphology findings. Two tier approach for correct characterization of abnormal hemoglobins of HPLC &/or capillary electrophoresis.
I have listed out the LE cells structure and Microscopical examinaton of LE CELLS, Difference between tart cells and le cells, clinical symptoms and diagnostic procedure.
Fetal hemoglobin and rh incompatibilityrohini sane
A comprehensive presentation on fetal hemoglobin & Rh incompatibility for undergraduate medical, dental, biotechnology & pharmacology students for self-learning .Presentation has physical & chemical properties of fetal hemoglobin along with its function. Binding affinity for O₂ of HbF and oxygen dissociation curve for HbF elucidated with suitable diagrams. Molecular constitution of Embryonic Hb ( Grover I &Grover II )with electrophoretic patterns are presented here . Importance of Kleihauer staining for detection of fetal cells is described briefly.
Diagrammatic representation of Rh- incompatibility is done for complete understanding of the concept. Signs & symptoms Kernicterus are presented diagrammatically.
Direct and indirect Coomb’s Test for Rh- incompatibility for diagnosis of Erythroblastosis Fetalis is illustrated. Biochemical aspects of Hemolytic Disease of Newborn (HDN) and Physiological /Neonatal Jaundice are presented. Comparison of Causes & biochemical findings for Hemolytic Jaundice along hepatic and obstructive jaundice is done in this presentation.
Molecular mechanism involved in biosynthesis of Hb Bart and Hb H along with their electrophoretic patterns for their detection are illustrated.
Hereditary persistent fetal Hb( HPFH ) & Point mutations causing HPFH are described in lucid manner. Google images are used for intense impact of the subject.
Fetal hemoglobin and rh incompatibilityrohini sane
A comprehensive presentation on fetal hemoglobin & Rh incompatibility for undergraduate medical, dental, biotechnology & pharmacology students for self-learning .Presentation has physical & chemical properties of fetal hemoglobin along with its function. Binding affinity for O₂ of HbF and oxygen dissociation curve for HbF elucidated with suitable diagrams. Molecular constitution of Embryonic Hb ( Grover I &Grover II )with electrophoretic patterns are presented here . Importance of Kleihauer staining for detection of fetal cells is described briefly.
Diagrammatic representation of Rh- incompatibility is done for complete understanding of the concept. Signs & symptoms Kernicterus are presented diagrammatically.
Direct and indirect Coomb’s Test for Rh- incompatibility for diagnosis of Erythroblastosis Fetalis is illustrated. Biochemical aspects of Hemolytic Disease of Newborn (HDN) and Physiological /Neonatal Jaundice are presented. Comparison of Causes & biochemical findings for Hemolytic Jaundice along hepatic and obstructive jaundice is done in this presentation.
Molecular mechanism involved in biosynthesis of Hb Bart and Hb H along with their electrophoretic patterns for their detection are illustrated.
Hereditary persistent fetal Hb( HPFH ) & Point mutations causing HPFH are described in lucid manner. Google images are used for intense impact of the subject.
As long as knowledge on inherence, diagnosis and preventive measures are limited to very few people, it is difficult to control the spread of the genetic anomaly in our population. Apart from lack of comprehensive knowledge, The findings in this study showed a high level of general awareness about the existence of SCD but comprehensive knowledge about the cause and prevention was low and associated with vast misconceptions. A large percentage did not see its importance in influencing their marital decisions. Perhaps simple interventions that worked in the western countries can also work in India.
Electrophoresis, the types of electrophoresis and samples usedMohit Adhikary
The different types of electrophoresis, and the different types of electrophoresis are explained here, along with the different samples that can be electrophoresed.
MOLECULAR PATHOGENESIS OF PREVALENT HEMOGLOBINOPATHIESChelsea Osayande
Hemoglobinopathies are congenital disorders resulting from hemoglobin abnormalities. Major forms are often severe, their management is difficult and associated with a great psychosocial impact on patients and their families.
These hemoglobinopathies may be due to alterations in certain globin chains that include:
Absence of production
Diminished production
Abnormal structure.
The clinically significant hemoglobinopathies include α- and β-thalassemia, sickle cell disease, HbE disease, and HbC disease.
1. The concept of the internal environment.
2. Homeostasis. The concept of the norm, hard and plastic constants.Homeokinez.
3. General characteristics of blood. Hematokrit and its clinical evaluation.
4. Functions of blood.
5. The chemical composition of the plasma.
6. Plasma electrolytes and their significance.
Vaccines What is Vaccine? What are the different types of vaccines?Maneesha M Joseph
What is Vaccine Malayalam What are the different types of vaccines?#Malayalam Lecture class #vaccine
VACCINES
A vaccine is a medical preparation given to provide immunity from a disease.
Vaccines use a variety of different substances ranging from dead microorganisms to genetically engineered antigens to defend the body against potentially harmful microorganisms.
Effective vaccines change the immune system by promoting the development of antibodies that can quickly and effectively attack disease-causing microorganisms when it enters the body, preventing disease development.
A vaccine may contain live-attenuated or killed microorganisms or parts or products from them capable of stimulating a specific immune response comprised of protective antibodies and T cell immunity.
A vaccine should stimulate a sufficient number of memory T and B lymphocytes to yield effector T cells and antibody-producing B cells from memory cells.
The viral vaccines should also be able to stimulate high titers of neutralizing antibodies.
Injection of a vaccine into a nonimmune subject induces active immunity against the modified pathogens.
Vaccination is immunization against infectious disease through the administration of vaccines for the production of active (protective) immunity in humans or other animals
There are 4 main types of vaccines:
Live Attenuated vaccines (LAV)
Inactivated vaccines (Killed Antigen)
Subunit and Conjugate Vaccines (Purified Antigen)
Toxoid vaccines (Inactivated Toxins)
1. Live attenuated (LAV)
Tuberculosis (BCG), Oral polio vaccine (OPV), Measles, Rotavirus, Yellow fever
2. Inactivated (killed antigen)
Whole-cell pertussis (wP), Inactivated polio virus (IPV)
3. Subunit (purified antigen)
Acellular pertussis (aP),
Haemophilus inuenzae type b (Hib), Pneumococcal (PCV-7, PCV-10, PCV-13), Hepatitis B (HepB)
4. Toxoid (inactivated toxins)
Tetanus toxoid (TT), Diphtheria toxoid
Vaccines teach the immune system to fight off disease by helping it learn what a pathogen looks like. What kinds of vaccines are out there, and how do they differ from one another?
Vaccines and the Immune Response: How Vaccines Work
This animation provides an overview of vaccines and the immune response, and how influenza vaccines work. Influenza vaccines are able to trigger an immune response by mimicking viral infection. They are usually manufactured using inactivated or killed virus particles taken from various circulating influenza strains.
Qualification
Maneesha M Joseph
MSc MLT (Microbiology)
Assistant Professor
Baby memorial college of allied Health science
Kozhikode
Bacterial Growth Curve microbiology | Log phase lag phase stationary phase in microbial growth
bacterial growth curve microbiology
In this video, I will be discussing what the bacterial growth curve reflects, different phases & their significance.
Bacterial growth curve and microbial growth kinetics - This microbiology lecture is going to teach you about the bacterial growth curve and the microbial growth kinetics. The microbial growth curve consists of 4 phases.
Lag phase of the growth curve, Log phase of the bacterial growth curve which is also known as the exponential growth phase and the stationary phase of bacterial growth curve where the bacterial growth is about to stop. The last phase of microbial growth curve is the death phase where the cell death is complete and the bacterial population starts declining.
Stay tuned to know more about the bacterial growth curve microbiology.
Thank you for watching the video lecture on microbial growth curve microbiology.
As the channel name suggests, our channel will be a perfect lounge for the malayali medicos..we wil be covering videos which will be like lecture classes related to the subjects biochemistry and microbiology in which we are specialised.. It will be a better learning experience for the students especially for those who are not able to understand and follow the normal classes in college..we assure the students that you will get a basic idea regarding the topic and extra reading can be done from the reference textbooks...
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Qualification
Maneesha M Joseph
MSc MLT (Microbiology)
Assistant Professor
Baby memorial college of allied Health science
Kozhikode
Our Partner Channel
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#Mallu
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Bacterial Growth Curve
ACTINOMYCES - Causes, Symptoms, Diagnosis, Pathogenesis & Treatment #Actinomycosis
Actinomyces are a Gram-positive anaerobic type of bacteria. At one time, due to their fungus-like branched network of hyphae, they were assumed to be a type of fungus. They play an important role in soil ecology by producing enzymes that help degrade organic plant materials.
The bacteria are known for causing disease in humans and animals and are one of most common causes of infections following dental procedures as the bacteria can be found in the nose and throat of many people. The infectious bacterial disease caused by Actinomyces species is known as actinomycosis.
Actinomycosis is a rare type of infectious bacterial disease. Unlike other infections, actinomycosis is able to move gradually and infiltrate the body's tissue, causing swelling and inflammation. Eventually there is tissue damage and scaring, pus-filled abscesses appear in the mouth, lungs, or gastrointestinal tract. Small holes leaking pus form in the affected tissue.
Actinomycosis is caused by a species of bacteria known as actinomyces, which live harmlessly in the lining of the mouth, throat, digestive system and women´s vagina. If the tissue-lining becomes damaged by injury or disease, the bacteria can penetrate deeper into the body - the actinomyces bacteria then thrive deep inside human tissue.
This bacterial disease is very rare in humans, but more common in farm animals. In cattle it is called lumpy jaw
As the channel name suggests, our channel will be a perfect lounge for the malayali medicos..we wil be covering videos which will be like lecture classes related to the subjects biochemistry and microbiology in which we are specialised.. It will be a better learning experience for the students especially for those who are not able to understand and follow the normal classes in college..we assure the students that you will get a basic idea regarding the topic and extra reading can be done from the reference textbooks...
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Qualification
Maneesha M Joseph
MSc MLT (Microbiology)
Assistant Professor
Baby memorial college of allied Health science
Kozhikode
Our Partner Channel
Health & Voyage channel link - https://youtu.be/nzKqRVjlwc0
#ACTINOMYCES - Causes, Symptoms, Diagnosis, Treatment, Pathology
# Actinomycosis
#Mallu
#Microbiology
#Biochemistry
#MalluMedicosLounge
#HealthAndVoyage
Parvovirus B19 - Causes, Symptoms, Pathology, Diagnosis, Treatment #Parvovirus B19
As the channel name suggests, our channel will be a perfect lounge for the malayali medicos..we wil be covering videos which will be like lecture classes related to the subjects biochemistry and microbiology in which we are specialised.. It will be a better learning experience for the students especially for those who are not able to understand and follow the normal classes in college..we assure the students that you will get a basic idea regarding the topic and extra reading can be done from the reference textbooks...
If you like my video
like
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Qualification
Maneesha M Joseph
MSc MLT (Microbiology)
Assistant Professor
Baby memorial college of allied Health science
Kozhikode
Our Partner Channel
Health & Voyage channel link - https://youtu.be/nzKqRVjlwc0
#Mallu
#Microbiology
#Biochemistry
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#parvovirus treatment
WIDAL Test Microbiology - Principle, Procedure, Limitations, Results, QC #WidalTest
As the channel name suggests, our channel will be a perfect lounge for the malayali medicos..we wil be covering videos which will be like lecture classes related to the subjects biochemistry and microbiology in which we are specialised.. It will be a better learning experience for the students especially for those who are not able to understand and follow the normal classes in college..we assure the students that you will get a basic idea regarding the topic and extra reading can be done from the reference textbooks...
If you like my video
Like
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don't forget to subscribe my channel
Qualification
Maneesha M Joseph
MSc MLT (Microbiology)
Assistant Professor
Baby memorial college of allied Health science
Kozhikode
Our Partner Channel
Health & Voyage channel link - https://youtu.be/nzKqRVjlwc0
#WidalTest
#Microbiology
#Biochemistry
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#HealthAndVoyage
#Test Microbiology
Oxidase Test Microbiology - Principle, Procedure, Limitations, Results, QC - in lab #Oxidase Test
As the channel name suggests, our channel will be a perfect lounge for the malayali medicos..we wil be covering videos which will be like lecture classes related to the subjects biochemistry and microbiology in which we are specialised.. It will be a better learning experience for the students especially for those who are not able to understand and follow the normal classes in college..we assure the students that you will get a basic idea regarding the topic and extra reading can be done from the reference textbooks...
If you like my video
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Qualification
Maneesha M Joseph
MSc MLT (Microbiology)
Assistant Professor
Baby memorial college of allied Health science
Kozhikode
Our Partner Channel
Health & Voyage channel link - https://youtu.be/nzKqRVjlwc0
#Oxidase Test
#Medical
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# malayalam lecturer
#Mallu Medicos Lounge
#MalluMedicosLounge
#MLT
Superficial Mycoses Mycology - Tinea Versicolor / Tinea Nigra/Piedra
For Downloading PDF note
As the channel name suggests, our channel will be a perfect lounge for the malayali medicos..we wil be covering videos which will be like lecture classes related to the subjects biochemistry and microbiology in which we are specialised.. It will be a better learning experience for the students especially for those who are not able to understand and follow the normal classes in college..we assure the students that you will get a basic idea regarding the topic and extra reading can be done from the reference textbooks..
Qualification
AHLAD T O
Maneesha M Joseph
MSc MLT (Microbiology)
Assistant Professor
Baby memorial college of allied Health science
Kozhikode
Our Partner Channel
Health & Voyage channel link - https://youtu.be/nzKqRVjlwc0
#Superficial Mycoses Mycology microbiology
#Medical
#Microbiology
#Superficial Mycoses Mycology malayalam lecturer
#Mallu Medicos Lounge
##MalluMedicosLounge
#MLT
#Tinea Versicolor
#Tinea Nigra
#Piedra
Methyl Red (MR) and Voges-Proskauer (VP) Test principle, Method, Interpretation & QC #MR & VP
Mallu Medicos Lounge
As the channel name suggests, our channel will be a perfect lounge for the malayali medicos..we wil be covering videos which will be like lecture classes related to the subjects biochemistry and microbiology in which we are specialised.. It will be a better learning experience for the students especially for those who are not able to understand and follow the normal classes in college..we assure the students that you will get a basic idea regarding the topic and extra reading can be done from the reference textbooks..
Our Partner Channel
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Qualification
Maneesha M Joseph
MSc MLT (Microbiology)
Assistant Professor
Baby memorial college of allied Health science
Kozhikode
#Methyl Red Test
#Voges-Proskauer Test
#MRVP Procedure
#MRVP Tests
#MR-VP Tests
#VP (Voges Proskaeur) Test
#MR/VP Test
#Methyl red (MR) and Voges-Proskauer (VP)
#Methyl red test in Microbiology
#Medical
#Microbiology
#MR/VP Test malayalam lecturer
#Mallu Medicos Lounge
#MalluMedicosLounge
#MLT
#Channel introduction
#HealthAndVoyage
IMMUNE RESPONSE - Humoral Immune Response
As the channel name suggests, our channel will be a perfect lounge for the malayali medicos..we wil be covering videos which will be like lecture classes related to the subjects biochemistry and microbiology in which we are specialised.. It will be a better learning experience for the students especially for those who are not able to understand and follow the normal classes in college..we assure the students that you will get a basic idea regarding the topic and extra reading can be done from the reference textbooks..
Qualification
AHLAD T O
MSc MLT (Biochemistry)
Assistant Professor
Baby memorial college of allied Health science
Kozhikode
Maneesha M Joseph
MSc MLT (Microbiology)
Assistant Professor
Baby memorial college of allied Health science
Kozhikode
Our Partner Channel
Health & Voyage channel link - https://youtu.be/nzKqRVjlwc0
#IMMUNE RESPONSE microbiology
#Medical
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#Biochemistry
#Mallu Medicos Lounge
##MalluMedicosLounge
#MLT
#Channel introduction
#HealthAndVoyage
#New Youtube Channel introduction
#Gram-negative
#Enterobactericea
#Weil Felix Test
#Humoral Immune Response
#Humoral Immune Response Tutorial
What is Klebsiella? Klebsiella is a Gram-negative rod-shaped bacteria, which belongs to a family of bacteria called the Enterobacteriaceae.
As the channel name suggests, our channel will be a perfect lounge for the malayali medicos..we wil be covering videos which will be like lecture classes related to the subjects biochemistry and microbiology in which we are specialised.. It will be a better learning experience for the students especially for those who are not able to understand and follow the normal classes in college..we assure the students that you will get a basic idea regarding the topic and extra reading can be done from the reference textbooks..
Maneesha M Joseph
MSc MLT (Microbiology)
Assistant Professor
Baby memorial college of allied Health science
Kozhikode
Our Partner Channel
Health & Voyage channel link - https://youtu.be/nzKqRVjlwc0
#Klebsiella
#Medical
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#Channel introduction
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#New Youtube Channel introduction
#Klebsiella pneumoniae
As the channel name suggests, our channel will be a perfect lounge for the malayali medicos..we wil be covering videos which will be like lecture classes related to the subjects biochemistry and microbiology in which we are specialised.. It will be a better learning experience for the students especially for those who are not able to understand and follow the normal classes in college..we assure the students that you will get a basic idea regarding the topic and extra reading can be done from the reference textbooks..
Qalification
AHLAD T O
MSc MLT (Biochemistry)
Assistant Professor
Baby memorial college of allied Health science
Kozhikode
Maneesha M Joseph
MSc MLT (Microbiology)
Assistant Professor
Baby memorial college of allied Health science
Kozhikode
Our Partner Channel
Health & Voyage channel link - https://youtu.be/nzKqRVjlwc0
#Proteus microbiology
#Medical
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#Channel introduction
#HealthAndVoyage
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#Gram-negative
#Enterobactericea
#Weil Felix Test
#PROTEUS - causes, symptoms, diagnosis, treatment, pathology
HOT NEW PRODUCT! BIG SALES FAST SHIPPING NOW FROM CHINA!! EU KU DB BK substit...GL Anaacs
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The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
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Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
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2. ELECTROPHORESIS
It is the process of moving charged biomolecules in an electrolyte solution by applying an electrical field
across the mixture.
Biomolecules will move with a speed, dependent on their charge, shape, and size. separation occures on
the basis of their molecular size.
Electrophoresis is used: for analysis and purification of very large molecules (proteins, nucleic acids)
for analysis of simpler charged molecules (sugars, amino acids, peptides, nucleotides, and simpler ions).
3. When charged molecules are placed in an electric field, they migrate toward either the positive (anode) or
negative (cathode) pole according to their charge.
Factors influenced electrophoresis mobility:
1.net charge of the molecule
2.size and shape
3.concentration of the molecule in solution
SUPPORTING MEDIA
Solid matrix with pores which are used:
• paper
• starch
• cellulose acetate
• polyacrylamide
• agar/agarose
Molecules in the sample move through porous matrix at
different velocity.
4. BUFFERS
Function of buffer
1. carries the applied current
2. established the pH
3. determine the electric charge on the solute
High ionic strength of buffer
produce sharper band
produce more heat
Commonly used buffer
Barbitone buffer & Tris-EDTA for protein
Tris-acetate-EDTA & Tris-borate-EDTA (50mmol/L; pH 7.5-7.8)
5. HEMOGLOBIN ELECTROPHORESIS
It is a blood test that can detect different types of hemoglobin. It uses the principles
of cellulose acetate & gel electrophoresis to separate out the various types of hemoglobin.
The electrophoresis process takes advantage of the fact that hemoglobin types have different
electrical charges. During electrophoresis, an electrical current is passed through the
hemoglobin in a blood sample, which causes the hemoglobin types to separate at different rates
and form bands
By comparing the pattern formed with that of a normal blood sample, doctors can see the types
and quantities of hemoglobin present in the blood sample
6. The test can detect abnormal levels of HbS, the form associated with sickle-cell disease, as well as
other abnormal hemoglobin-related blood disorders, such as hemoglobin C.
It can also be used to determine whether there is a deficiency of any normal form of hemoglobin, as in
the group of diseases known as thalassemias.
The hemoglobin electrophoresis is also known to be thalessemia screening, this also can be helpful
for the patient who is frequently need of fresh blood transfusion. The patient needs blood transfusion
because the body is unable to produce enough hemoglobin to satisfy the body's requirement
Hemoglobin, the oxygen-carrying protein inside red blood cells, comes in many molecular forms,
some normal and some abnormal. Normal hemoglobin carries and releases oxygen efficiently, while
abnormal hemoglobin doesn't.
7. THE TYPES OF HAEMOGLOBIN
Hemoglobin F: This type is found in growing fetuses and newborns. Soon after birth, it is
replaced with hemoglobin A.
Hemoglobin A: This is the most common type of hemoglobin found in healthy children and
adults.
Hemoglobin C, D, E, M, and S: These (and many other, rarer variations) are types of abnormal
hemoglobin.
If a person inherits the genes that cause production of too much of an abnormal type of
hemoglobin, or not enough normal hemoglobin, it can lead to blood disorders due to inherited
haemoglobin abnormalities.
8. NORMAL VALUES
IN ADULTS:
Hgb A1: 95% to 98%
Hgb A2: 2% to 3%
Hgb F: 0.8% to 2%
Hgb S: 0%
Hgb C: 0%
IN INFANTS AND CHILDREN:
Hgb F (newborn): 50% to 80%
Hgb F (6 months): 8%
Hgb F (over 6 months): 1% to 2%
9. THERE ARE 3 MAIN CATEGORIES OF INHERITED HAEMOGLOBIN
ABNORMALITIES:
1) Structural or qualitative:The amino acid sequence is altered because of
incorrect DNA code(Hemoglobinopathy)
2) Quantitative: Produdction of one or more globin chain is reduced or
absent(Thalassemia)
3) Hereditary persistence of Fetal haemoglobin(HPFH).complete or partial failure
of γ globin to switch to β globin
PATHOLOGICAL VARIANTS
• Hemoglobin H(β4)-A variant form of Hb formed by a tetramer of β chains
which may be present in variants of α thalassemia.
• Hemoglobin Barts (γ4)-A variant form of Hb formed by a tetramer of γ chains
which may be present in variants of α thalassemia.
10. • Hemoglobin S (α2βS2)-A variant form of Hb found in people with sickle cell disease .In
the 6th position of globin chain glutamic acid is substituted by valine.
Under low oxygen conditions, absence of a polar amino acid in the β chain
promotes polymerisation of Hb,which distorts RBC to sickle shape and decrease their
elasticity.Sickle cells block the flow of blood in narrow capillaries.Interruption in oxygen
supply leads to localised anoxia in the tissues causing cell death.
• Hemoglobin C (α2βC2) – Another variant due to variation in the β chain gene. In the 6th
position of globin chain glutamic acid is substituted by lysine.This variant causes mild
chronic haemolytic anemia. HbC is slower moving than HbA on electrophoresis at alkaline
PH.
11. • Hemoglobin E (α2βE2) - Another variant due to variation in the β chain gene.This
variant causes mild chronic haemolytic anemia.
• Hemoglobin AS – A heterozygous form causing sickle cell trait with one adult
gene and one sickle cell disease gene.
• Hemoglobin SC disease – A compound heterozygous form with one sickle gene
and another encoding Hemoglobin C
• Hemoglobin D – Also called as HbD Punjab. Results from replacement of beta 121
glutamic acid by glutamine. HbD migrates similar to HbS on electrophoresis at
alkaline PH
12. LABORATORY INVESTIGATIONS TO DETECT ABNORMAL HEMOGLOBIN
I ) Red cell morphology
HbS – sicke cells
HBC – Target cells ,crystals after splenectomy
Thalassemias – Microcytosis,Target cells,Basophilic stipplings
13. II ) Hemoglobin Electrophoresis
Cellulose Acetate At Alkaline pH
Citrate Agar Electrophoresis at Acid pH
III) Iso electric focusing
A pH gradient is established by carrier ampholytes submitted to an electric current and the Hb
molecules migrate across this gradient until they reach the position where their net charge is zero
(isoelectric point). The molecules will then concentrate in a sharp band
IV) High Performance Liquid Chromatography
Weak cation exchange column is used.The ionic strength of eluting solution is gradually increased
and causes the various Hb variants to have a particular retension time.
14. PRINCIPLE OF CELLULOSE ACETATE ELECTROPHORESIS
In an alkaline pH (8.2-8.6) Hb is a negatively charged molecule and will migrate
towards the anode (+). The various Hbs moves at different rates depending on their net
negative charge, which in turn is controlled by the composition (amino acids) of the Hb
molecule (globin chain).
The red cell hemolysate (red blood cell membranes are destroyed to free the Hb
molecules for testing) is placed in a cellulose acetate membrane, which is positioned in
an electrophoresis tray with the inoculated hemolysate near the cathode (-).
One end of the cellulose acetate strip is immersed in the buffer (pH 8.2-8.6) on the
cathode side and the other end is placed in the buffer on the anode (+) side. An electric
current of specific voltage is allowed to run for a timed period.
During electrophoresis, the Hb molecules migrate toward the anode because of their
negative charge. The difference in the net charge of the Hb molecule determines its
mobility and manifests its self by the speed with which it migrates to the positive pole.
15. The cellulose acetate membrane is then stained in order to color the proteins (Hbs). By
noting the distance each Hb has migrated and comparing this distance with the migration
distance of known controls, the types of hemoglobins may be identified.
Example of the fast Hbs are Hb Bart’s and the tow fastest variants Hb H and I, while Hb C
is the slowest common Hb.
16. REQUIREMENTS
Haemolysate (a haemolysate is the contents of the red cells)
Hemolysate are prepared by addition of water and toluene to saline washed erythrocytes
Tris-EDTA Boric Acid (TEB) buffer, pH 8.4.
Whatman No. 3 chromatography paper.
Cellulose acetate membranes
HbA2 control, as supplied by the National Institute for Biological Standards and Control NIBSC.
The control has been produced by freeze drying a solution of haemoglobin prepared from human cells and
made stable by the addition of sucrose (200 mM), potassium cyanide (6mM) and chloramphenicol (1 mg/dl).
This control was established by the World Health Organization in 1994.
Protein stain solution (0.5% Ponceau S in 5% TCA)
Destain solution (5% acetic acid)
17. PROCEDURE
1.Place TEB electrophoresis buffer (about 500 ml total) into all compartments of the electrophoresis tank.
Ensure that the level is the same in all compartments by carefully lifting the tank so that the buffer laps over
the end of the separating walls. Wipe any excess liquid from the walls with a tissue.
2.Thoroughly wet 2 pieces of Whatman No. 3 filter paper (20 x 7.5 cm) with buffer solution and place them
over the edges of the shoulder pieces with one edge of the paper running parallel with edge of the shoulder
and the other immersed in the buffer of the outer compartment. These pads act as buffer wicks between the
buffer solution and the cellulose acetate strips which are placed between them.
3.Take one cellulose acetate strip and, with a pencil label at one end. Also mark a faint starting line (origin)
lightly across the centre of the strip.
18. 4.Moisten the strip as follows. Add some TEB buffer to a shallow dish and carefully float the
strip on top so that it is impregnated with buffer from below by capillary action. When the
strip is thoroughly wetted (3-4 min), it can then be submerged in the buffer.
5.Remove excess buffer from the strip by blotting lightly on filter paper — do not overdry by
pressing too hard.
6.Apply the sample applicator to the surface of hemolysate then transfer the sample to origin
line on the strip by gently touching the applicator on to the strip .Similarly transfer the control
also to the strip
7.Place the strip between the two shoulder pads of the electrophoresis tank, with the origin in
the centre and the end with label towards the anode and carefully pull taut. Press the ends of
the strip firmly against the pad to ensure proper contact
19. 8.Place the Perspex lid on the tank and connect the red and black terminals of the tank to the
+ve and -ve terminals of the power supply.
9.Electrophoresis will be carried out at a constant voltage of 150 V (approx. 0.5 mA/cm
strip width) for 60 mins.
10.Remove the strip with forceps and carefully float it on the surface of the Ponceau S
staining solution, allowing the stain to impregnate the strip from below. When totally
wetted, immerse the strip completely in the stain and leave for 5 mins. Agitate occasionally.
11.To destain, drain off excess stain and rinse in a tray of 5% acetic acid. Change the acetic
acid once, agitate for a moment, then finally rinse the strip in tap water.
20.
21.
22.
23. INTERPRETATION
Control in Alkaline electrophoresis: H A F S A2
Sickle Trait
This is a heterozygous state showing HbA and HbS and a normal amount of HbA2 on cellulose acetate. Results on
citrate agar show hemoglobins in the HbA and HbS migratory positions (zones).
Sickle Cell Anemia
This is a homozygous state showing almost exclusively HbS, although a small amount of HbF may also be present
Sickle-C Disease
This is a heterozygous state demonstrating HbS and HbC.
Thalassaemia Major
This condition shows HbF, HbA and HbA2.
24. Sickle Cell –Thalassaemia Disease:
This condition shows HbA, HbF, HbS and HbA2.
Hemoglobin-C Disease:
Disease This is a homozygous state showing almost exclusively HbC
Thalassemia-C Disease:
This condition shows HbA, HbF, and HbC
25. CITRATE AGAR ELECTROPHORESIS ( ACID PH)
Citrate agar separates Hb fractions that migrate together on cellulose acetate agar.
All Hb specimens that show an abnormal electrophoretic pattern in alkaline media (cellulose
acetate agar) should undergo electrophoresis on an acid citrate agar.
Citrate agar electrophoresis at acid pH (6.0 to 6.2) permits separation of the major
hemoglobins based on the relative mobilities of variant hemoglobins: Hb C from Hb E and Hb O,
as well as Hb S from Hb D and Hb G.
However, this electrophoresis will not distinguish Hb E from Hb O, nor Hb D from Hb G.
Hemoglobin bands are visualized by staining with amido black and acid violet .
The procedure should not be used as a screening procedure because many
abnormal Hbs migrate with Hb A.
26. Electrophoresis of Hbs on agar at acidic pH is not primarily sensitive to the charge of the
mutated residue but to structural modifications of positively charged regions of the Hb molecule
interacting with the agaro pectin contained in the gel. This property is of special interest to
distinguish Hb S from other variants displaying mobility close to that of Hb S on CAE or IEF.
This procedure is the method of choice when examining newborns (cord blood specimens) and
infants under 3 months of age for some abnormal Hbs such as S and C because the test is able to
detect quantities of Hb not easily seen by other techniques
27. Citrate agar electrophoresis is carried out on plates of the Helena type. Agar 1gm is suspended
in 100 ml of citrate buffer and heated to boiling.
Plates were poured by pipetting 10 ml of the hot agar into plastic trays
(3 x 3.75 inches) and allowing the agar to gel.
Plates are stored at 4 C prior to use.
Hemolysate (patient sample & control) is applied on to the gel.The hemolysate reagent
contains 0.005 M EDTA in deionized water with 0.07% potassium cyanide added as a
preservative.
Citrate agar runs are done at room temperature using approximately 40 ma/plate.
Plates are run for 45 minutes followed by staining, using o-dianisidine and hydrogen peroxide.
Stained plates are washed with 5% acetic acid and dried on cards for storage and photography
PROCEDURE
29. C S A F
D
G
A2
E
O
CITRATE AGAR ELECTROPHORESIS PATTERN
30.
31.
32. WHEN IT IS DONE?
A doctor may order hemoglobin electrophoresis to help diagnose diseases
(called hemoglobinopathies) involving abnormal hemoglobin production, such
as sickle cell disease and thalassemia.
Doctors also may order the test when a child has family history of a
hemoglobinopathy or is found to have anemia that isn't due to a more common
cause, such as iron deficiency.
In many states, a hemoglobin electrophoresis is performed as part of a series of
newborn screening blood tests, primarily so that kids with sickle cell anemia
can be diagnosed, monitored, and treated early to prevent potentially life-
threatening complications.