This document discusses various analytical instrumentation and methods. It begins by explaining the objectives of the lecture, which are to explain the principles of methods, discuss factors that may affect the methods, and know the importance of the methods. It then discusses several analytical techniques including colorimetry, spectrophotometry, electrophoresis, chromatography, electrochemistry, and atomic absorption spectroscopy. It provides examples and diagrams to illustrate the principles and components involved in these important analytical methods.

1. INSTRUMENTATION

2. QUOTE FOR THE DAY

3. OBJECTIVES
• AT THE END OF THE LECTURE, YOU
SHOULD BE ABLE TO:
• 1. EXPLAIN THE PRINCIPLES OF THE
METHODS.
• 2. DISCUSS THE FACTORS THAT MAY
AFFECT THE METHODS.
• 3. KNOW THE IMPORTANCE OF THESE
METHODS TO THE PROFESSION.

4. COLORIMETRY
• VISUAL
• PHOTOELECTRIC

5. SPECTRONIC 20 D

6. CELL
WELL
ON/OF
CONTROL
0 ABSORBANCE
CONTROL
ABSORBANCE /
TRANSMITTANCE
CONTROL
DISPLAY WINDOW
LED DISPLAY
MODE
BUTTONS
INCREASE
DECREASE
CONCENTRATION
BUTTON
WAVELENGTH
CONTROL

7. BEER LAMBERT’S LAW

9. SPECTROPHOTOMETRY
• BEER LAMBERT’S
LAW O.D
Cu
% T
O.D.
Cu
% T

10. Linear Graphing Paper

11. WHICH ONE IS MORE CONCENTRATED, BASED ON COLOR?
INTENSITY OF COLOR

12. v-frequency

13. wl

14. WAVELENGTHS
Infra red
visible
ultraviolet

15. Schematic of a single beam
spectrophotometer

16. ELECTROPHORESIS

18. Gel Electrophoresis

20. PAPER CHROMATOGRAPHY

21. Completed Paper Chromatography of a
Plant Pigment

22. ELECTROCHEMISTRY

23. TURBIDIMETRY

24. EMISSION FLAME PHOTOMETRY

25. EFP
ASPIRATOR
ATOMIZER
FLAME

26. VOLUMETRIC & TITRIMETRIC

27. GRAVIMETRIC

28. NEPHELOMETRY

29. FLUOROMETRY

31. ATOMIC ABSORPTION
SPECTROSCOPY

32. AAS

33. AAS

34. AAS

35. MASS
SPECTROPHOTOMETRY

36. OSMOMETRY

38. CHEMILUMINESCENCE

39. BRING OUT ¼ SHEET OF YELLOW PAPER

40. POST QUIZ
• 1. Method that measures blocked light
• 2. Method that applies the separation of substances
using mobile and stationary phase.
• 3. Give the relationship of size to electrophoretic
mobility.
• 4. In chromatography, what is the stationary
phase?
• 5. Give one method that applies the separation of
ions in an electric field.
• 6. Range of IR light.
• 7. Range of visible light.
• 8.-10 Name factors that affect EFP.
• 11-15.Trascribe IEC,PC,GC,RBS, FBS

41. 1. Method that measures Absorbed light
2. Method that applies the separation of
charged particles in an electric field.
3. Give the relationship of density to
electrophoretic mobility.
4. In chromatography, what is the mobile
phase?
5. Give one method that separates
ions/atoms from one another.
6. Range of visible light.
7. Range of uv light.
8.-10 Name factors that affect
electrophoresis method