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Presentation
“DNA Replication”
*
History of DNA replication
Make copies of DNA
DNA Replication means to
DNA Replication happens when
new cells need to be made
i. James Watson & Francis
Crick suggest the first correct
double-helix model of DNA
structure.
ii. Meselson-Stahl experiment
confirms replication mechanism as
implied by the double-helical
structure.
*
Introduction of DNA replication
A.Helicase
B.DNA Polymerase
C.Primase
D.Ligase
A.Initiation
B.Elongation
C.Termination
Description of enzyme
Replication fork:
Primer:
Primase:
Helicase:
• Where the DNA strands split off
• Moves with the path of the replication
bubble
Short sequence of RNA that binds to the
lagging parental strand in order for DNA
polymerase to copy the lagging strnad
A protein that binds to the parental lagging
strand to make a primer
A protein that unwinds DNA
Single strand binding protein:
DNA ligase:
DNA polymerase:
A protein that binds each DNA strand to
stabilize it and hold it apart
A protein that adds free nucleotides from
5’ to 3’ to make a new strand of DNA.
Also fixes mistakes in DNA
A protein that binds the phosphates of
nucleic acids to the sugar group of
adjacent nucleic acids
How does DNA replication
How does DNA replicate?
Application of DNA replication
*
 DNA is unique to each individual, and therefore can
be used for identification purposes.
 The use of biotechnology has completely
revolutionized medicine. Now it is much easier to
generate vaccines than it was previously, and more
effective vaccines can be made through
biotechnology.
 DNA technology and sequencing allows us to
perform DNA tests to determine paternity, to
calculate the probability of developing a genetic
disorder
 To identify a missing person or
murderer
 Cloning
 CRISPR
*
Dna replication

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