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Peripheral smear
Preparations

• Specimen-direct capillary blood or
  anticoagulated blood
• Apparatus-clean glass slides,lancet,
  spreade...
Composition of leishman’s stain

•   Polychromed methylene blue
•   Eosin azure leishman powder
•   Acetone free absolute ...
Procedure
MAKING A SMEAR
• One drop of blood on one end of slide
• Spreader slide is placed at 45°on the drop
  and moved ...
Staining a smear

• Place the smear on the staining rack
• Pour leishman stain to cover the smear
  completely allow to fi...
Examination of the smear

• The stained slide is placed on the
  microscope and is focused under low
  power
• Place a dro...
Precautions
• Objective must be clean
• Objective must be cleaned after every use
• Good smear is tongue shaped occupying
...
Diagnostic value
RBC’s SERIES
• Size-microcytic/normocytic/macrocytic/
  anisocytosis
• Shape-poikilocytosis,sickle cells,...
WBC’s SERIES

DIFFERENTIAL COUNT
• Neutrophills-40--70%
• Eosinophills-2--4 %
• Basophills-0--1 %
• Monocytes-2--4 %
• Lym...
Immature cells



• Myeloid series-
  myeloblasts,promyelocyte,myelocyte,met
  amyelocyte,band forms
• Lymphoid series-lym...
Thank you
PLATELET SERIES

• General score-6--10platelets/oil
  immersion field

               PARASITES
• Intracellular-malarial p...
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Peripheral Smear Using Leishman Stain

PBS using Leishman Stain

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Peripheral Smear Using Leishman Stain

  1. 1. Peripheral smear
  2. 2. Preparations • Specimen-direct capillary blood or anticoagulated blood • Apparatus-clean glass slides,lancet, spreader slide,microscope&microscope oil • Stain-leishman stain,wright or gimsa • Diluent-distilled water or buffer solution with Ph 6.8
  3. 3. Composition of leishman’s stain • Polychromed methylene blue • Eosin azure leishman powder • Acetone free absolute alcohol • 1.5gms leishman powder in 1 liter acetone free absolute alcohol
  4. 4. Procedure MAKING A SMEAR • One drop of blood on one end of slide • Spreader slide is placed at 45°on the drop and moved along the slide • It is moved smoothly and once such that the blood film is thin • Care should be taken to prevent the formation of air bubbles • Air dry the smear • Make identification mark on one edge
  5. 5. Staining a smear • Place the smear on the staining rack • Pour leishman stain to cover the smear completely allow to fix for 2-3 mins • Add water twice the amount of leishman’s stain allow to fix for 7-10 mins • Appearance of golden scum or sheen on the surface of stain • Wash the stain off the slide with running water • Wipe the back of slide and air dry the slide
  6. 6. Examination of the smear • The stained slide is placed on the microscope and is focused under low power • Place a drop of oil on the slide and observe under oil immersion objective • Observe RBC,platelet series • WBC series is observed and a differential count is done • Record the findings
  7. 7. Precautions • Objective must be clean • Objective must be cleaned after every use • Good smear is tongue shaped occupying 2/3rd of the slide • Divided into head,body and tail • Must be focused at the junction of body and tail
  8. 8. Diagnostic value RBC’s SERIES • Size-microcytic/normocytic/macrocytic/ anisocytosis • Shape-poikilocytosis,sickle cells,target cells,tear drop cells,burr cells spherocytosis,ovalocytosis • Color-hypochromic/normochromic • Immature cells- normoblasts,polychromatophillic cells
  9. 9. WBC’s SERIES DIFFERENTIAL COUNT • Neutrophills-40--70% • Eosinophills-2--4 % • Basophills-0--1 % • Monocytes-2--4 % • Lymphocytes-25—40 %
  10. 10. Immature cells • Myeloid series- myeloblasts,promyelocyte,myelocyte,met amyelocyte,band forms • Lymphoid series-lymhoblasts
  11. 11. Thank you
  12. 12. PLATELET SERIES • General score-6--10platelets/oil immersion field PARASITES • Intracellular-malarial parasite • Extracellular-microfilarial

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